Optimization for the Production of Surfactin with a New Synergistic Antifungal Activity

-surfactin and the optimization of its production by the response surface method.O. A production of 134.2 mg/L, which were in agreement with the prediction, was observed in a verification experiment. In comparison to the production of original level (88.6 mg/L), a 1.52-fold increase had been obtained.-surfactin

Dominância fiscal : uma investigação empírica sobre o caso brasileiro no período de 2003 a 2014

A estabilização econômica dos anos de 1990 e a adoção do tripé econômico, a partir de 1999, marcam o fim de um capítulo delicado da história brasileira; a partir de então, era necessária a existência de certa sintonia de políticas monetária e fiscal para a manutenção do controle dos diversos indicadores econômicos. Contudo, com essa reciprocidade na política econômica, são incitadas discussões sobre a orientação do governo na hora de definir suas prioridades nesse campo: as variáveis fiscais são priorizadas e, por conseguinte, determinadas, forçando as monetárias a se ajustarem – ou o contrário? A resposta para esse questionamento leva à discussão sobre a dominância fiscal. Assim, esse trabalho visa verificar empiricamente, usando das modelagens econométricas VAR e estudo de eventos, se há dominância fiscal ou monetária na economia brasileira e se a eficácia da política monetária mudou na transição do governo Lula para o governo Dilma. O resultado foi inconclusivo para o governo Lula e indicou dominância fiscal no governo Dilma. Ainda verificou-se não haver modificação na eficácia da política monetária.Economic stabilization, in the 1990s, and utilization of an economic tripod, after 1999, represents the end of a delicate chapter in Brazilian history. Ever since, it was necessary the existence of a certain agreement between monetary and fiscal politic, in order to maintain under control a variety of economic indicators. However, this reciprocity (in economic politic) starts discussions about the real government orientations when it comes to define its priority on this subject: are the fiscal variables priorized, and then, determined, forcing monetary variables to adjust themselves, or the opposite? The answer to these questions emerge from the fiscal dominance discussion. This paper intends to empiric verify, using econometric modeling VAR and event study, if there is fiscal dominance or monetary in Brazilian economy and whether the effectiveness of monetary politic has changed in the transition from Lula's government to the Dilma government. The result was inconclusive for the Lula government and indicated fiscal dominance in the Dilma government. There was still no change in the efficiency of the monetary politic.CAPE

Production and characterization of iturinic lipopeptides as antifungal agents and biosurfactants produced by a marine Pinctada martensii-derived Bacillus mojavensis B0621A

Bacillus mojavensis B0621A was isolated from a pearl oyster Pinctada martensii collected from South China Sea. While screening for cyclic lipopeptides potentially useful as lead compounds for biological control against soil-bone fungal plant pathogens, three lipopeptides were isolated and purified from the fermentation broth of B. mojavensis B0621A via vacuum flash chromatography coupled with reversed-phase high performance liquid chromatography (RP-HPLC). The structural characterization and identification of these cyclic lipopeptides were performed by tandem mass spectrometry (MS/MS) combined with gas chromatography-mass spectrometry (GC-MS) analysis as well as chemical degradation. These lipopeptides were finally characterized as homologues of mojavensins, which contained identical amino acids back bones of asparagine1, tyrosine2, asparagine3, glutamine4, proline5, asparagine6, and asparagine7 and differed from each other by their saturated β-amino fatty acid chain residues, namely, iso-C14 mojavensin, iso-C16 mojavensin, and anteiso-C17 mojavensin, respectively. All lipopeptide isomers, especially iso-C16 mojavensin and anteiso-C17 mojavensin, displayed moderate antagonism and dose-dependent activity against several formae speciales of Fusarium oxysporum and presented surface tension activities. These properties demonstrated that the lipopeptides produced by B. mojavensis B0621A may be useful as biological control agent to fungal plant pathogens

Production and characterization of surfactin-type lipopeptides as bioemulsifiers produced by a Pinctada martensii-derived Bacillus mojavensis B0621A

Bacillus mojavensis B0621A was isolated from the mantle of a pearl oyster Pinctada martensii collected from South China Sea. Semi-purified surfactins (225 mg L-1) were obtained by acid precipitation and vacuum flash chromatography. The component of the semi-purified surfactins was preliminarily analyzed by liquid chromatography mass spectrometer system, and the results showed that all these surfactins could be a group of homologues. Eight surfactin homologues were isolated and afforded by reversed phase high-performance liquid chromatography. Furthermore, their structure was characterized by mass spectrometry analysis combined with nuclear magnetic resonance spectroscopy techniques. These surfactins shared seven amino acids as peptide backbone and a saturated beta-hydroxy fatty acid chain residue (from C-13 to C-15), differed each other from peptide sequence in the position of Leu(7) or Val(7). All these surfactins had significant activity and stability of emulsification under various pH (from 7.0 to 12.0), temperature range (from 20 to 115 A degrees C) and sodium chloride concentration (from 2.5 to 20.0 %, w/v). Taken all together, these results indicated that B. mojavensis B0621A have potential to be an alternative source as a biological-derived emulsifying agent

Isolation and characterization of a new iturinic lipopeptide, mojavensin A produced by a marine-derived bacterium Bacillus mojavensis B0621A

Three lipopeptides were isolated by bioactivity-guided fractionation from the fermentation broth of Bacillus mojavensis B0621A. A new iturinic lipopeptide, named mojavensin A, was tentatively characterized by 1D, 2D NMR and MS spectroscopy, Marfey's method containing a novel peptide backbone of L-Asn(1), D-Tyr(2), D-Asn(3), L-Gln(4), L-Pro(5), D-Asn(6), L-Asn(7) and an anteiso-type of the saturated beta-fatty acid side chain. Compound 2 and 3 were tentatively identified as iso-C16 fengycin B and anteiso-C17 fengycin B, respectively. These lipopeptides displayed dose-dependent antifungal activity against a broad spectra of phytopathogens and were weakly antagonistic to Staphylococcus aureus. Moreover, they all revealed cytotoxic activities against the human leukemia (HL-60) cell line. Mojavensin A, iso-C16 fengycin B, and anteiso-C17 fengycin B inhibited the growth of HL-60 with IC(5)(0) of 100, 100 and 1.6 muM, respectively

High-Quality Development Path of the "Enclave Economy" from the Perspective of Symbiosis: A Case Study of Guangqing Economic Cooperation Zone (Guangde Park)

The "enclave economy" is an important governance tool for promoting regional integration. Guangdong Province occupies the leading position in exploring the "enclave economy" model in China, which has effectively promoted the development of the east and northwest of Guangdong Province through the "point to area" approach. However, certain problems that are associated with the "enclave economy" concept, including a weak level of industrial relatedness, disconnection with the development of the local urban system, and inadequate interest driving mechanisms, need to be urgently addressed. The article aims to explore the high-quality development path of the "enclave economy" in the new era, taking advantage of the symbiosis theory. Based on field research and semi-structured interview methods, the present study attempts to not only probe into the cooperative relationship and mutually beneficial symbiosis mechanism among different actors in the "enclave economy" but also explore a more sustainable and mutually beneficial development "enclave economy" model. With respect to theoretical contribution, based on the symbiosis theory and the characteristics of the "enclave economy", this study facilitates a better understanding of the high-quality development logic of the "enclave economy" from the perspective of symbiosis in particular and establishes a theoretical framework comprising the "industrial symbiosis network-industrial symbiosis unit-interest symbiosis mechanism" components. Empirically, this study takes the Guangqing Economic Cooperation Zone (Guangde Park) as the case study; reveals the development process and problems of the "enclave economy", including the isolation of industry transplantation, fragmentation of industry-city units, and locking of interests and mismatch between rights and responsibilities; and proposes high-quality development strategies, such as establishing a cross-regional "enclave economic circle", a resource-linking platform between industry and city, and a community of interests in industrial parks and towns, to form a more sustainable symmetric and mutually beneficial symbiotic mechanism. The key contribution of this study lies in the theoretical framework for the high-quality development of the "enclave economy" through the lens of symbiosis, which enriches the ways of analysis and cognitive logic of the "enclave economy" and advances cross-regional cooperation and "enclave economy" research. It enhances the cross-territorial geographic thinking of regional cooperation and "enclave economy" research, holding the potential to provide decision-making references for the promotion of regional coordinated development in Guangdong Province in the new period. It also has practical application value for relieving the pressure of unbalanced and insufficient regional development in China by promoting cross-municipal and cross-provincial cooperation

Novel Antioxidants and α-Glycosidase and Protein Tyrosine Phosphatase 1B Inhibitors from an Endophytic Fungus Penicillium brefeldianum F4a

Oxidative stress plays a very important role in the progression of diabetes and its complications. A therapeutic agent that is both antidiabetic and antioxidant would be the preferred choice for the treatment of diabetes. The crude extract of the endophytic fungus Penicillium brefeldianum F4a has significant antioxidant and α-glycosidase and protein tyrosine phosphatase 1B (PTP1B) inhibition activities. Chemical investigation of P. brefeldianum F4a using an activity-guided isolation led to the discovery of three new compounds called peniorcinols A–C (1–3) along with six known compounds: penialidins A (4), penialidin F (5), myxotrichin C (6), riboflavin (7), indole-3-acetic acid (8), and 2-(4-hydroxy-2-methoxy-6-methylphenyl) acetic acid (9). Their chemical structures were established by their NMR and HRESIMS. The absolute configurations of 1 and 3 were determined by experimental and calculated electronic circular dichroism (ECD). Their antioxidant activities were evaluated by DPPH• and ABTS•+ scavenging assays. Compounds 1–6 and 8–9 showed moderate to strong free radical scavenging activities. Significantly, 4–6 exhibited more potent ABTS•+ scavenging activity than that of the positive control. Their α-glycosidase and PTP1B inhibition activities were tested. Among them, compound 3 showed α-glucosidase inhibition activity, and compounds 7 and 8 showed PTP1B inhibitory activity for the first time. It is worth noting that 3 and 8 displayed both antioxidant and α-glycosidase or PTP1B inhibition activities. These finding suggest that compounds 3 and 8 could be used as lead compounds to generate new potent drugs for the treatment of oxidative stress-related diabetes

Study on the Bending Effect and Rock Burst Mechanism of Middle Rock Pillars in Extremely Thick Subvertical Coal Seams

Rock bursts occur in nearly vertical coal seam mines at shallow to moderate burial depths, which endangers safe mining. To study the rock burst mechanisms of nearly vertical and extremely thick coal seams, the characteristics of rock bursts were studied via on-site investigation, and a field test of in situ stress was carried out. The mechanical behavior of rock pillars in the middle of the B1+2 and B3+6 coal seams was analyzed using theoretical and numerical simulation methods. The results show that the horizontal maximum principal stress orientation and the nearly vertical coal seam strike were both 82°. The bending of the rock pillars occurred due to the horizontal unbalanced force, and a large amount of bending energy was accumulated within 50 m above the mining level. Rock pillars were bent toward the B1+2 mining goaf and exerted a reverse bending and squeezing effect on the B3+6 coal seam below the mining levels. In addition to the inclination and compression of the B3+6 coal seam roof, stress concentration zones formed in the B3+6 coal seam, where a large amount of elastic energy had accumulated in the coal-rock mass. Consequently, both the rock pillars and the B3+6 coal body at the mining level are in an unstable state undue to mining disturbance. Rock burst energy theory and numerical calculation results showed that in the stress concentration zones of the B3+6 coal seam, the energy density of the coal mass reached or exceeded its critical value before rock burst occurred, and rock bursts were prone to occur under mining disturbances. The in situ microseismic results showed that high-energy microseismic events were mainly concentrated in middle rock pillars around the mining levels and the coal mass in high-stress concentration zones