Use of Traditional Chinese Medicine and Its Impact on Medical Cost among Urban Ischemic Stroke Inpatients in China: A National Cross-Sectional Study

Background. Traditional Chinese medicine (TCM) has long been widely adopted by the Chinese people and has been covered by China’s basic medical insurance schemes to treat ischemic stroke. Previous research has mainly highlighted the therapy effect of TCM on ischemic stroke patients. Some studies have demonstrated that employing TCM can reduce the medical burden on other diseases. But no research has explored whether using TCM could reduce inpatient medical cost for ischemic stroke in mainland China. The purpose of this study is to investigate the impact of the use of TCM on the total inpatient cost of ischemic stroke and to explore whether TCM has played the role of being complementary to, or an alternative for, conventional medicine to treat ischemic stroke. Methods. We conducted a national cross-sectional analysis based on a 5% random sample from claims data of China Urban Employee Basic Medical Insurance (UEBMI) and Urban Resident Basic Medical Insurance (URBMI) schemes in 2015. Mann–Whitney test was used to compare unadjusted total inpatient cost, conventional medication cost, and nonpharmacy cost estimates. Ordinary least square regression analysis was performed to compare demographics-adjusted total inpatient cost and to examine the association between TCM cost and conventional medication cost. Results. A total of 47321 urban inpatients diagnosed with ischemic stroke were identified in our study, with 92.6% (43843) of the patients using TCM in their inpatient treatment. Total inpatient cost for TCM users was significantly higher than TCM nonusers (USD 1217 versus USD 1036, P<0.001). Conventional medication cost was significantly lower for TCM users (USD 335 versus USD 436, P<0.001). The average cost of TCM per patient among TCM users was USD 289. Among TCM users, conventional medication costs were found to be positively associated with TCM cost after adjusting for confounding factors (Coef. = 0.144, P<0.001). Conclusion. Although the use of TCM reduced the cost of conventional medicine compared with TCM nonusers, TCM imposed an extra financial component on the total inpatient cost on TCM users. Our study suggests that TCM mainly played a complementary role to conventional medicine in ischemic stroke treatment in mainland China

Sedimentary ancient DNA reveals past ecosystem and biodiversity changes on the Tibetan Plateau: Overview and prospects

Alpine ecosystems on the Tibetan Plateau are being threatened by ongoing climate warming and intensified human activities. Ecological time-series obtained from sedimentary ancient DNA (sedaDNA) are essential for understanding past ecosystem and biodiversity dynamics on the Tibetan Plateau and their responses to climate change at a high taxonomic resolution. Hitherto only few but promising studies have been published on this topic. The potential and limitations of using sedaDNA on the Tibetan Plateau are not fully understood. Here, we (i) provide updated knowledge of and a brief introduction to the suitable archives, region-specific taphonomy, state-of-the-art methodologies, and research questions of sedaDNA on the Tibetan Plateau; (ii) review published and ongoing sedaDNA studies from the Tibetan Plateau; and (iii) give some recommendations for future sedaDNA study designs. Based on the current knowledge of taphonomy, we infer that deep glacial lakes with freshwater and high clay sediment input, such as those from the southern and southeastern Tibetan Plateau, may have a high potential for sedaDNA studies. Metabarcoding (for microorganisms and plants), metagenomics (for ecosystems), and hybridization capture (for prehistoric humans) are three primary sedaDNA approaches which have been successfully applied on the Tibetan Plateau, but their power is still limited by several technical issues, such as PCR bias and incompleteness of taxonomic reference databases. Setting up high-quality and open-access regional taxonomic reference databases for the Tibetan Plateau should be given priority in the future. To conclude, the archival, taphonomic, and methodological conditions of the Tibetan Plateau are favorable for performing sedaDNA studies. More research should be encouraged to address questions about long-term ecological dynamics at ecosystem scale and to bring the paleoecology of the Tibetan Plateau into a new era

Imagined Common Ground: Rethinking on Language, Translation and Technology

When waking up from the dream of one-world-ness, how do we talk to each other? Technological development and the hegemonic definition of modernity that emerged from it have been under interrogation for decades. In a diminishingly globalized world, we are prompted to reflect on what our connectedness brought to us. There is a world free to roam for some, but not for all. The transnational corporations export their imagined world of synchrony, although it was also those trades and exchanges in the history that cultivated new imaginations (and sometimes violence). Could it be our sense of entitlement to the “feel-at-home”, the immediacy promised by the newest technologies, and our inability to hear and talk without aided translations, that contribute to a singular world under the name of “international”? The end of one world marks the emergence of many worlds, seen or unseen. This thesis is an attempt to respond to those questions of the global imagination and concern through its projection on a contemporary “nation,” China. It starts with the formation of the Han Chinese identity, itself a construct that cannot be reduced to a singular image. The case I write about is the distinct culture of Sichuan, formed by the past millennial influx of immigration. The thesis continues to unravel the complexity of the Chinese language, namely the separation of the oral and the written, the hierarchy of the vernacular oral (dialects) and the official oral (Mandarin), and the transience of the vernacular written. Translation happens on multiple levels, yet through the untranslatability of the unwritten to the written, a culture shaped by locality survives and mutates. The thesis also investigates how technology shapes the Chinese language in the digital age, and how standardization could possibly curb the liveliness of the unwritten or alter its living trajectory. The text ends with a discussion of personal anecdotes, weaving the writing and artistic practices together.S.M

Statistically Consistent Inverse Optimal Control for Linear-Quadratic Tracking with Random Time Horizon

The goal of Inverse Optimal Control (IOC) is to identify the underlying objective function based on observed optimal trajectories. It provides a powerful framework to model expert\u27s behavior, and a data-driven way to design an objective function so that the induced optimal control is adapted to a contextual environment. In this paper, we design an IOC algorithm for linear-quadratic tracking problems with random time horizon, and prove the statistical consistency of the algorithm. More specifically, the proposed estimator is the solution to a convex optimization problem, which means that the estimator does not suffer from local minima. This enables the proven statistical consistency to actually be achieved in practice. The algorithm is also verified on simulated data as well as data from a real world experiment, both in the setting of identifying the objective function of human tracking locomotion. The statistical consistency is illustrated on the synthetic data set, and the experimental results on the real data shows that we can get a good prediction on human tracking locomotion based on estimating the objective function. It shows that the theory and the model have a good performance in real practice. Moreover, the identified model can be used as a control target in personalized rehabilitation robot controller design, since the identified objective function describes personal habit and preferences

Construction of pVAX-WIF-1 Eukaryotic Expression Vector and Its Anti-tumor Effect on Lung Cancer

Background and objective WIF-1 is an important tumor-suppressing gene in lung cancer, and its encoding protein WIF-1 can reduce proliferation and promote apoptosis by inhibiting Wnt/β-catenin signaling in lung cancer. This study constructs a eukaryotic expression plasmid carrying WIF-1 using FDA-approved clinical plasmid pVAX and explores the anti-tumor effect of pVAX-WIF-1 on A549 lung cancer cells in vitro and vivo. Methods The DNA fragment of human WIF-1 coding sequence was amplified by PCR and was cloned into the multiple cloning sites of eukaryotic expression vector pVAX to construct pVAX-WIF-1. A recombinant plasmid was transfected into lung cancer A549 cells, and the expression of WIF-1 genes was verified by Western blot after transfection. Subsequently, the effect of pVAX-WIF-1 on cell apoptosis and proliferation was identified by MTT assay, staining A549 cells with Hoechst 3235, and flow cytometry. Finally, the A549 subcutaneous xenograft was established to detect the effect of pVAX-WIF-1 on lung tumor growth in vivo. Results The results of restriction enzyme digestion, PCR, and sequencing indicated that eukaryotic expression plasmid pVAX-WIF-1 was successfully constructed. The protein expression level of WIF-1 was increased in the transfected A549 cells. Further results showed that transfection with pVAX-WIF-1 significantly inhibited proliferation and promoted apoptosis in A549 cells. Moreover, pVAX-WIF-1 significantly inhibited the tumor growth of the A549 subcutaneous xenograft in vivo. Conclusion The recombinant eukaryotic expression vector pVAX-WIF-1 was successfully constructed. Transfection with pVAX-WIF-1 could significantly inhibit proliferation and promote apoptosis of lung cancer A549 cells and also effectively inhibit the tumor growth of the A549 subcutaneous xenograft in vivo. Our research can contribute to clinical applications of WIF-1 in lung cancer gene therapy

Identification and validation of core genes in tumor-educated platelets for human gastrointestinal tumor diagnosis using network-based transcriptomic analysis

Gastrointestinal (GI) tumors have increasing incidence worldwide with their underlying mechanisms still not being fully understood. The use of tumor-educated platelets (TEPs) in liquid biopsy is a newly-emerged blood-based cancer diagnostic method. Herein, we aimed to investigate the genomic changes of TEPs in GI tumor development and their potential functions using network-based meta-analysis combined with bioinformatic methods. We used a total of three eligible RNA-seq datasets, which were integrated using multiple meta-analysis methods on the NetworkAnalyst website, and identified 775 DEGs (differentially expressed genes; 51 up-regulated and 724 down-regulated genes) in GI tumor relative to healthy control (HC) samples. These TEP DEGs were mostly enriched in bone marrow-derived cell types and associated with gene ontology (GO) of “carcinoma” and could affect pathways of “Integrated Cancer Pathway” and “Generic transcription pathway” respectively for highly and lowly expressed DEGs. Combined network-based meta-analysis and protein–protein interaction (PPI) analysis identified cyclin dependent kinase 1 (CDK1) and heat shock protein family A (Hsp70) member 5 (HSPA5) to be the hub genes with the highest degree centrality (DC), being up-regulated and down-regulated in TEPs, respectively. GO and Kyoto Encyclopedia of Genes and Genomes (KEGG) results showed that the hub genes were primarily related to cell cycle and division, nucleobase-containing compound and carbohydrate transport, and endoplasmic reticulum unfolded protein response. Additionally, the nomogram model suggested that the two-gene signature owns extraordinary predictive power for GI tumor diagnosis. Further, the two-gene signature was demonstrated to have potential value for metastatic GI tumor diagnosis. The expression levels of CDK1 and HSPA5 in clinical platelet samples were verified to be consistent with the results from bioinformatic analysis. This study identified a two-gene signature encompassing CDK1 and HSPA5 that can be used as a biomarker for GI tumor diagnosis and maybe even cancer-associated thrombosis (CAT)-related prognosis

Carbon-Coated Na₃V₂(PO₄)₃ Anchored on Freestanding Graphite Foam for High-Performance Sodium-Ion Cathodes

Na₃V₂(PO₄)₃ (NVP) has been considered as a most promising cathode material for sodium-ion batteries (SIBs), but NVP usually exhibits poor cycling stability and rate performance due to the low intrinsic electrical conductivity. Herein, we prepared carbon-coated Na₃V₂(PO₄)₃ anchored on freestanding graphite foam (denoted as NVP@C-GF) as a cathode for SIBs. The NVP@C-GF exhibits superior sodium-ion storage performance, including rate capability (56 mAh g^–1 at 200 C) and long cycle life (54 mAh g^–1 at 100 C after 20 000 cycles). The resulting NVP@C-GF inherits the advantages of 3D free-standing graphite that possesses high electrical conductivity and porous structure for the electrolyte to soak in. Furthermore, carbon-coated NVP particles anchored on the surface of GF not only accommodate the volume change of NVP during charge/discharge but also reduce the diffusion distance of the Na⁺ ion