Extensive analysis of D7S486 in primary gastric cancer supports TESTIN as a candidate tumor suppressor gene

<p>Abstract</p> <p>Background</p> <p>High frequency of loss of heterozygosity (LOH) was found at D7S486 in primary gastric cancer (GC). And we found a high frequency of LOH region on 7q31 in primary GC from China, and identified D7S486 to be the most frequent LOH locus. This study was aimed to determine what genes were affected by the LOH and served as tumor suppressor genes (TSGs) in this region. Here, a high-throughput single nucleotide polymorphisms (SNPs) microarray fabricated in-house was used to analyze the LOH status around D7S486 on 7q31 in 75 patients with primary GC. Western blot, immunohistochemistry, and RT-PCR were used to assess the protein and mRNA expression of TESTIN (TES) in 50 and 140 primary GC samples, respectively. MTS assay was used to investigate the effect of TES overexpression on the proliferation of GC cell lines. Mutation and methylation analysis were performed to explore possible mechanisms of TES inactivation in GC.</p> <p>Results</p> <p>LOH analysis discovered five candidate genes (<it>ST7</it>, <it>FOXP2</it>, <it>MDFIC</it>, <it>TES </it>and <it>CAV1</it>) whose frequencies of LOH were higher than 30%. However, only <it>TES </it>showed the potential to be a TSG associated with GC. Among 140 pairs of GC samples, decreased <it>TES </it>mRNA level was found in 96 (68.6%) tumor tissues when compared with matched non-tumor tissues (<it>p </it>< 0.001). Also, reduced TES protein level was detected in 36 (72.0%) of all 50 tumor tissues by Western blot (<it>p </it>= 0.001). In addition, immunohistochemical staining result was in agreement with that of RT-PCR and Western blot. Down regulation of TES was shown to be correlated with tumor differentiation (<it>p </it>= 0.035) and prognosis (<it>p </it>= 0.035, log-rank test). Its overexpression inhibited the growth of three GC cell lines. Hypermethylation of <it>TES </it>promoter was a frequent event in primary GC and GC cell lines. However, no specific gene mutation was observed in the coding region of the <it>TES </it>gene.</p> <p>Conclusions</p> <p>Collectively, all results support the role of <it>TES </it>as a TSG in gastric carcinogenesis and that <it>TES </it>is inactivated primarily by LOH and CpG island methylation.</p

Catalytic Hydrogen Evolution of NaBH4_4 Hydrolysis by Cobalt Nanoparticles Supported on Bagasse-Derived Porous Carbon

As a promising hydrogen storage material, sodium borohydride (NaBH4) exhibits superior stability in alkaline solutions and delivers 10.8 wt.% theoretical hydrogen storage capacity. Nevertheless, its hydrolysis reaction at room temperature must be activated and accelerated by adding an effective catalyst. In this study, we synthesize Co nanoparticles supported on bagasse-derived porous carbon (Co@xPC) for catalytic hydrolytic dehydrogenation of NaBH$_4$. According to the experimental results, Co nanoparticles with uniform particle size and high dispersion are successfully supported on porous carbon to achieve a Co@150PC catalyst. It exhibits particularly high activity of hydrogen generation with the optimal hydrogen production rate of 11086.4 mL$_{H2}$∙min$^{H2}$∙g$_{Co}$$^{H2}$ and low activation energy (E$_{a}$) of 31.25 kJ mol$^{H2}$. The calculation results based on density functional theory (DFT) indicate that the Co@xPC structure is conducive to the dissociation of [BH$_{4}$]$^{-}$, which effectively enhances the hydrolysis efficiency of NaBH$_4$. Moreover, Co@150PC presents an excellent durability, retaining 72.0% of the initial catalyst activity after 15 cycling tests. Moreover, we also explored the degradation mechanism of catalyst performance

Throughput Multiplexing Efficiency for High-Order Handset MIMO Antennas

In multipath environments, multiple-input multiple-output (MIMO) terminals typically suffer from non-zero correlations due to limited handset space and power imbalances due to different antenna efficiencies or partial hand blockage. These antenna–channel impairments can lead to significant throughput performance degradation of MIMO terminals. The multiplexing efficiency was proposed to quantitatively assess this performance degradation. Previous work only derived the analytical expression of the throughput-based multiplexing efficiency of two-port MIMO antennas for user equipment (UE). However, the fifth-generation (5G) UE dictates four or more antennas. In this paper, we extend the multiplexing efficiency metric to high-order MIMO UEs. Both correlation-based channel models and geometry-based stochastic channel models were used for validation. Besides dipole antennas, two representative terminal antennas were also employed for simulation verifications in this work

Throughput Multiplexing Efficiency for High-Order Handset MIMO Antennas

In multipath environments, multiple-input multiple-output (MIMO) terminals typically suffer from non-zero correlations due to limited handset space and power imbalances due to different antenna efficiencies or partial hand blockage. These antenna&ndash;channel impairments can lead to significant throughput performance degradation of MIMO terminals. The multiplexing efficiency was proposed to quantitatively assess this performance degradation. Previous work only derived the analytical expression of the throughput-based multiplexing efficiency of two-port MIMO antennas for user equipment (UE). However, the fifth-generation (5G) UE dictates four or more antennas. In this paper, we extend the multiplexing efficiency metric to high-order MIMO UEs. Both correlation-based channel models and geometry-based stochastic channel models were used for validation. Besides dipole antennas, two representative terminal antennas were also employed for simulation verifications in this work

Anion Exchange Membrane Based on Interpenetrating Polymer Network with Ultrahigh Ion Conductivity and Excellent Stability for Alkaline Fuel Cell

A high-performance anion exchange membrane (AEM) is critical for the development of alkaline fuel cell. In this work, AEMs with an interpenetrating polymer network (IPN) are synthesized. An electron microscope clearly reveals a highly efficient “ion channel” network, which is constructed with a small amount of cation exchange groups. This specially designed ion channel leads to extraordinary hydroxide conductivity (e.g., 257.8 mS cm-1 at 80 °C) of IPN AEMs at moderate ion exchange capacity (IEC=1.75 mmol g−1), as well as excellent long-term alkaline stability at harsh condition which showed that 81% of original conductivity can be retained after a long time for 1248 hours. Moreover, a remarkable peak power density of 1.20 W cm-2 (0.1 MPa backpressure) with nonprecious metal (FeNx-CNTs) as oxygen reduction reaction (ORR) catalyst in a fuel cell test was achieved. This work offers a general strategy to prepare high-performance AEMs based on IPN structure design

Primary research on a binder extraction method for Asphalt mix modified by using seamtm Asphalt mix modifier

Paper presented at the 26th Annual Southern African Transport Conference 9 - 12 July 2007 "The challenges of implementing policy?", CSIR International Convention Centre, Pretoria, South Africa. ABSTRACT: The existing bitumen extraction methods, including solvent extraction, the ignition method, the nuclear asphalt content device and biological resolving were analysed and examined for their applicability in determining the asphalt and SEAM contents of asphalt mixtures modified with sulphur-extended asphalt modifier. In SEAM-modified mixtures, sulphur separates out scatters in the mixture and dissolves in asphalt at a temperature lower than 115 °C. Sulphur can dissolve in sodium hydroxide and react chemically with new materials. In this study, the NaOH solvent extraction method was chosen to determine the SEAM content. The conventional solvent extraction method was used to determine the total amount of asphalt and SEAM. Detailed procedures and the testing precision of the NaOH solvent extraction method are given. The test method proposed in this study can be used in highway construction to control the SEAM content.This paper was transferred from the original CD ROM created for this conference. The material on the CD ROM was published using Adobe Acrobat technology. The original CD ROM was produced by Document Transformation Technologies Postal Address: PO Box 560 Irene 0062 South Africa. Tel.: +27 12 667 2074 Fax: +27 12 667 2766 E-mail: [email protected] URL: http://www.doctech.co.z

An epidemiological study of etiology and clinical characteristics in patients with nontraumatic osteonecrosis of the femoral head

Background: Osteonecrosis of the femoral head (ONFH) is a common disease with high disability rate. However, a few studies investigate the etiology and clinical characteristics of nontraumatic ONFH patients in China. Therefore, we conducted this cross-sectional study. Materials and Methods: Totally, information of 7268 nontraumatic ONFH patients treated between August 2005 and August 2015 was extracted from the medical records. The extracted information included the age, gender, diagnostic criteria, cause of nontraumatic ONFH, types of steroid use, and types of alcohol. Results: Among these included patients, there were 5126 (70.5%) male patients with average age of 44.5 years and 2142 (29.5%) female patients with average age of 47.6 years (P = 0.54). The number of steroid-, alcohol-, steroid/alcohol-, and idiopathic-induced nontraumatic ONFH men patients was 1684, 2310, 364, and 768, respectively, and nontraumatic ONFH women patients was 1058, 482, 140, and 462, respectively. Meanwhile, we found that both the levels of triglycerides (P = 0.03) and low-density lipoprotein (P = 0.02) were significantly changed in the idiopathic-induced nontraumatic ONFH patients. Conclusion: These results indicated the earlier onset of nontraumatic ONFH in male patients than in female patients, different main etiology for male (alcohol consumption) and female (steroid use) patients, and close relationship between the lipid metabolism and idiopathic-induced nontraumatic ONFH. Our findings could be helpful for researchers to investigate the pathogenesis of ONFH and aid the clinicians in the early prevention and diagnosis of nontraumatic ONFH

Competition or Cooperation? Exploring Unlabeled Data via Challenging Minimax Game for Semi-supervised Relation Extraction

Semi-Supervised Relation Extraction aims at learning well-performed RE models with limited labeled and large-scale unlabeled data. Existing methods mainly suffer from semantic drift and insufficient supervision, which severely limit the performance. To address these problems, recent work tends to design dual modules to work cooperatively for mutual enhancement. However, the consensus of two modules greatly restricts the model from exploring diverse relation expressions in unlabeled set, which hinders the performance as well as model generalization. To tackle this problem, in this paper, we propose a novel competition-based method AdvSRE. We set up a challenging minimax game on unlabeled data between two modules, Generator and Discriminator, and assign them with conflicting objectives. During the competition game, one module may find any possible chance to beat the other, which develops two modules' abilities until relation expressions cannot be further explored. To exploit label information, Discriminator is further asked to predict specific relation for each sentence. Experiment results on two benchmarks show new state-of-the-art performance over baselines, demonstrating the effectiveness of proposed AdvSRE

Preparation of Carbon-Covered Phosphorus-Modified Alumina with Large Pore Size and Adsorption of Rhodamine B

In this study, phosphorus-modified alumina with large pore size was synthesized through a coprecipitation method. The carbon-covered, phosphorus-modified alumina with large pores was prepared by impregnating with glucose and carbonizing to further improve the adsorption of organic dyes. The morphology and structure of these composites were characterized by various analysis methods, and Rhodamine B (RhB) adsorption was also examined in aqueous media. The results showed that the specific surface area and pore size of the phosphorus-modified alumina sample AP7 (prepared with a P/Al molar ratio of 0.07) reached 496.2 m2·g−1 and 21.9 nm, while the specific surface area and pore size of the carbon-covered phosphorus-modified alumina sample CAP7–27 (prepared by using AP7 as a carrier for glucose at a glucose/Al molar ratio of 0.27) reached 435.3 m2·g−1 and 21.2 nm. The adsorption experiment of RhB revealed that CAP7–27 had not only an equilibrium adsorption capacity of 198 mg·g−1, but also an adsorption rate of 162.5 mg·g−1 in 5 min. These superior adsorption effects can be attributed to the similar pore structures of CAP7–27 with those of alumina and the specific properties with those of carbon materials. Finally, the kinetic properties of these composites were also studied, which were found to be consistent with a pseudo-second-order kinetic model and Langmuir model for isothermal adsorption analysis. This study indicates that the prepared nanomaterials are expected to be promising candidates for efficient adsorption of toxic dyes

Enhanced Conductivity of Anion-Exchange Membrane by Incorporation of Quaternized Cellulose Nanocrystal

High ion conductivity of anion-exchange membrane is essential for the operation of alkaline anion-exchange membrane fuel cell. In this work, we demonstrated an effective strategy to enhance the conductivity of anion-exchange membrane (AEM), by incorporation of quaternized cellulose nanocrystal (QCNC) for the first time. Morphology observation demonstrated a uniform distribution of QCNC within QPPO matrix, as well as a clear QCNC network, which led to significant enhancement in hydroxide conductivities of composite membranes, for example, 2 wt % QCNC/QPPO membrane possessed a conductivity of 160% (60 mS cm^–1, @80 °C) of that of QPPO. Furthermore, H₂/O₂ cell performance of membrane electrode assembly based on 2 wt % QCNC/QPPO AEM showed an excellent peak power density of 392 mV cm^–2 at 60 °C without back pressure, whereas that of neat QPPO AEM was only 270 mV cm^–2