7 research outputs found
Enantioselective Michael Addition of Pyrroles with Nitroalkenes in Aqueous Media Catalyzed by a Water-Soluble Catalyst
A new
water-soluble catalytic system was developed and used in
an enantioselective Michael addition of pyrroles with nitroalkenes
in water to afford nitroethylpyrrole derivatives with both excellent
yields and ee values
Aryliodonium Salt-Induced Regioselective Access to <i>meta-</i>Substituted Anilines by Arylation of Azoles
A highly
efficient aryliodonium salt-induced regioselective access
to meta-substituted anilines by arylation of azoles
has been developed under catalyst-free conditions. This efficient
transformation provides a facile and scalable approach to a wide range
of biologically active N-arylazoles with moderate
to high yields. According to the control experiments, two plausible
pathways, including a Michael pathway and a free radical coupling
pathway, for the reaction were proposed
Coimmobilization of β‑Agarase and α‑Neoagarobiose Hydrolase for Enhancing the Production of 3,6-Anhydro‑l‑galactose
Here we report a simple and efficient
method to produce 3,6-anhydro-l-galactose (l-AHG)
and agarotriose (AO3) in one step
by a multienzyme system with the coimmobilized β-agarase AgWH50B
and α-neoagarobiose hydrolase K134D. K134D was obtained by AgaWH117
mutagenesis and showed improved thermal stability when immobilized
via covalent bonds on functionalized magnetic nanoparticles. The obtained
multienzyme biocatalyst was characterized by Fourier transform infrared
spectroscopy (FTIR). Compared with free agarases, the coimmobilized
agarases exhibited a relatively higher agarose-to-l-AHG conversion
efficiency. The yield of l-AHG obtained with the coimmobilized
agarases was 40.6%, which was 6.5% higher than that obtained with
free agarases. After eight cycles, the multienzyme biocatalyst still
preserved 46.4% of the initial activity. To the best of our knowledge,
this is the first report where two different agarases were coimmobilized.
These results demonstrated the feasibility of the new method to fabricate
a new multienzyme system onto magnetic nanoparticles via covalent
bonds to produce l-AHG
Clara Pitman and Alice Priest, Westbrook Seminary, Fall Term 1899
Clara Elizabeth Pitman (North Conway, N.H.) and Alice Priest (Canton, N.Y.) are listed in the Catalogue of the Officers and Students of Westbrook Seminary for the Academic Year Ending June 22, 1900, as Westbrook Seminary Class of 1901 and Westbrook Seminary Class of 1902, respectively.
In this 1899 sepia toned photograph mounted on a black backing, the two girls stand, with linked arms, outside Hersey Hall. Clara holds a plaid stadium blanket and Alice has a punching ball hanging from a cord around her neck. She leans her head on Clara\u27s shoulder. Both girls wear dark skirts and white blouses. Clara\u27s hair is piled Gibson Girl style on the top of her head.https://dune.une.edu/wchc_photos_students1900s/1002/thumbnail.jp
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Binding Kinetics Toolkit for Analyzing Transient Molecular Conformations and Computing Free Energy Landscapes
Understanding ligand binding kinetics
and thermodynamics,
which
involves investigating the free, transient, and final complex conformations,
is important in fundamental studies and applications for chemical
and biomedical systems. Examining the important but transient ligand–protein-bound
conformations, in addition to experimentally determined structures,
also provides a more accurate estimation for drug efficacy and selectivity.
Moreover, obtaining the entire picture of the free energy landscape
during ligand binding/unbinding processes is critical in understanding
binding mechanisms. Here, we present a Binding Kinetics Toolkit (BKiT)
that includes several utilities to analyze trajectories and compute
a free energy and kinetics profile. BKiT uses principal component
space to generate approximated unbinding or conformational transition
coordinates for accurately describing and easily visualizing the molecular
motions. We implemented a new partitioning approach to assign indexes
along the approximated coordinates that can be used as milestones
or microstates. The program can generate input files to run many short
classical molecular dynamics simulations and uses milestoning theory
to construct the free energy profile and estimate binding residence
time. We first validated the method with a host–guest system,
aspirin unbinding from β-cyclodextrin, and then applied the
protocol to pyrazolourea compounds and cyclin-dependent kinase 8 and
cyclin C complexes, a kinase system of pharmacological interest. Overall,
our approaches yielded good agreement with published results and suggest
ligand design strategies. The computed unbinding free energy landscape
also provides a more complete picture of ligand–receptor binding
barriers and stable local minima for deepening our understanding of
molecular recognition. BKiT is easy to use and has extensible features
for future expansion of utilities for postanalysis and calculations
Copper-Catalyzed Enantioselective Henry Reaction of β,γ‑Unsaturated α‑Ketoesters with Nitromethane in Water
A highly
enantioselective Henry reaction of β,γ-unsaturated
α-ketoesters with nitromethane in water by virtue of chiral
copper complexes has been developed. A series of unsaturated β-nitro-α-hydroxy
esters bearing tetrasubstituted carbon stereocenters were obtained
exclusively with high yields and excellent enantioselectivities. This
method could avoid tedious anaerobic anhydrous manipulation and reduce
the environmental pollution caused by organic solvents
Copper-Catalyzed Enantioselective Hetero-Diels–Alder Reaction of Danishefsky’s Diene with Glyoxals
The
highly enantioselective hetero-Diels–Alder reaction of Danishefsky’s
diene with glyoxals was developed by virtue of a readily accessible
chiral copper catalyst. This efficient transformation provided a facile
and scalable access to a wide range of biologically active dihydropyrones
with a high level of enantioselectivities. Moreover, the substrate
scope of this reaction could be extended to isatins with this catalytic
system. More importantly, the mechanism involved in this reaction
was proposed on the basis of the unambiguous structures of intermediates