Isolation and Identification of Acholeplasma sp. from the Mud Crab, Scylla serrata

For the first time, a mollicute-like organism (MLO) was cultured from moribund mud crabs (Scylla serrata) during an outbreak of clearwater disease in Zhejiang Province, China. The MLO displayed a fried-egg colony morphology in culture, did not possess a cell wall, and was not retained by 0.45 μm and 0.2 μm filters. It was able to ferment glucose, sucrose, lactose, and maltose, but it did not utilize arginine and urea. The MLO grew in the absence of bovine serum and was not susceptible to digitonin. Sequence analysis of the 16S rRNA gene revealed that this MLO had 99% identity with Acholeplasma laidlawii PG-8A, which indicates that the organism isolated from mud crabs is a member of the genus Acholeplasma

Inhibition of proliferation, migration and invasion of human non-small cell lung cancer cell line A549 by phlomisoside F from Phlomis younghusbandii Mukerjee

Purpose: To determine the effect of phlomisoside F (PMF) on the proliferation,  migration and invasion of human non-small cell lung cancer cell line A549 and explore the possible mechanisms.Methods: The anti-proliferative effect of PMF on A549 cells was determined by CCK-8. Subsequently, migration and invasion were evaluated by Transwell and Transwell with matrigel assays, respectively. Furthermore, cell cycle and apoptosis were assessed by flow cytometry, while the mechanisms of action were determined by Western blotting.Results: PMF exhibited significant anti-proliferative effect on A549 cells in  concentration-dependent and time-dependent manners, with half maximal inhibitory concentration (IC50) of 54.51 μM. Treatment with PMF (10, 20 and 40 μM) for 48 h resulted in significantly decreased migration and invasion in A549 cells. In addition, PMF at concentrations of 25, 50 and 75 μM induced cell cycle arrest in  G0/G1phase and enhanced cell apoptosis in A549 cells. Furthermore, caspase-3, caspase-9 and Bax protein expressions were up-regulated while Bacl-2 and COX-2 protein expressions were significantly downregulated at 10, 20 and 40 μM concentrations of PMF.Conclusion: PMF suppresses A549 cell growth, migration and invasion. The  mechanism may be related to the induction of mitochondria-mediated apoptosis pathway via regulation of caspase-3, caspase-9, Bcl-2 and Bax expressions, and inhibition of PGE2 synthesis by reducing COX-2 expression.Keywords: Phlomisoside F, Lung cancer, Cell mobility, Apoptosis, PGE2, COX-2 expression, Caspase, Cell cycle arres

Extraction and Recovery of Cerium from Rare Earth Ore by Solvent Extraction

Rare earth elements are widely found in many minerals, some of which, such as bastnaesite, monazite, and xenotime, are of great commercial value. Cerium (Ce) is the rare earth element with the highest content in light rare earth ore. Solvent extraction is the most effective and efficient method to recover and separate Ce from other light rare earth elements. After acid leaching of rare earth minerals, leaching solution was obtained, and cerium oxide of products of high purity was obtained by extraction and stripping. It is well known that Ce(IV) can be easily separated from the other RE(III) by adopting the traditional solvent extraction. Based on this principle, the clean process of oxidation roasting and Ce(IV) separation for Sichuan bastnaesite was developed. And then, a preliminary flow sheet of two-step oxidation and extraction of Ce(IV) for Bayan Obo mixed rare earth ores was further proposed

Exploring Cognitive Playfulness Through Zero Interactions

Many emerging technologies, products and services today try to use diverse methods of interaction to provide playful experiences. Increasingly more interactive features and techniques are being introduced to afford users new experiences and enrich our living environment. While many of these playful experiences can be achieved through various types of physical, sensory and social interactions, this paper attempts to focus on how 'no-interaction' can achieve playfulness in relation to our cognitive experience. If there is a way to give someone a playful experience without any physical, sensory and social interactions, where and how can we apply this approach or phenomenon? Here we share a provocation that tries to demonstrate a tangible means whereby such an idea could be used to explore potential user experiences within HCI

Pemanfaatan Susu Kambing Untuk Pembuatan Tahu Susu Dengan Penambahan Ekstrak Limbah Nenas (Enzim Bromelin) Dan Penambahan Ekstrak Daun Pepaya (Enzim Papain)

ABSTRAK Tahu susu adalah semacam keju yang dapat dibuat dari susu sapi, susu kambing atau susu hewan lainnya. Produk ini mempunyai nutrisi yang baik karena susu sebagai bahan pangan merupakan sumber protein hewani dengan susunan asam-asam amino yang lebih lengkap dibanding dengan protein dari tumbuh-tumbuhan. Penelitian ini bertujuan untuk meningkatkan konsumsi susu kambing dalam bentuk tahu susu untuk mencukupi kekurangan gizi masyarakat serta memanfaatkan limbah nenas dan daun pepaya dalam pembuatan tahu susu. Pembuatan tahu susu dengan penambahan ekstrak nenas dilakukan pada suhu 60°C dengan konsentrasi 12 ml, 14 ml, 16 ml dan 18 ml tiap 100 ml susu kambing, sedangkan pembuatan tahu susu dengan penambahan ekstrak pepaya dilakukan pada suhu 60° C dengan konsentrasi 11 ml, 13 ml, 15 ml tiap 100 ml susu kambing. Setelah diperoleh gumpalan tahu, dilakukan penyaringan dan pengepresan sehingga terbentuk tahu susu. Pembuatan tahu susu dengan penambahan 14 ml ekstrak limbah nenas (enzim bromelin) dan 15 ml ekstrak daun pepaya (enzim papain) memberikan hasil optimum berdasarkan tekstur dan rendemennya, selanjutnya digunakan untuk melakukan uji inderawi. Uji ini dilakukan terhadap warna, bau, kehalusan kelembutan saat dimakan, rasa dan kesukaan secara menyeluruh dengan metoda scoring test. Hasil uji inderawi terhadap warna, bau, kehalusan saat dimakan , rasa dan kesukaan secara menyeluruh menunjukkan bahwa tahu susu bromelin dan tahu susu papain tidak memberikan beda nyata dengan tahu susu kedelai dan tahu susu lain yang terdapat di pasaran. Hal ini menunjukkan bahwa tahu susu bromelin dan tahu susu papain dapat diterima dan disukai oleh konsumen

Superfluid and magnetic states of an ultracold Bose gas with synthetic three-dimensional spin-orbit coupling in an optical lattice

We study ultracold bosonic atoms with the synthetic three-dimensional spin-orbit (SO) coupling in a cubic optical lattice. In the superfluidity phase, the lowest energy band exhibits one, two or four pairs of degenerate single-particle ground states depending on the SO-coupling strengths, which can give rise to the condensate states with spin-stripes for the weak atomic interactions. In the deep Mott-insulator regime, the effective spin Hamiltonian of the system combines three-dimensional Heisenberg exchange interactions, anisotropy interactions and Dzyaloshinskii-Moriya interactions. Based on Monte Carlo simulations, we numerically demonstrate that the resulting Hamiltonian with an additional Zeeman field has a rich phase diagram with spiral, stripe, vortex crystal, and especially Skyrmion crystal spin-textures in each xy-plane layer. The obtained Skyrmion crystals can be tunable with square and hexagonal symmetries in a columnar manner along the z axis, and moreover are stable against the inter-layer spin-spin interactions in a large parameter region.Comment: 9 pages, 4 figures; title modified, references and discussions added; accepted by PR

Distributed Learning over Unreliable Networks

Most of today's distributed machine learning systems assume {\em reliable networks}: whenever two machines exchange information (e.g., gradients or models), the network should guarantee the delivery of the message. At the same time, recent work exhibits the impressive tolerance of machine learning algorithms to errors or noise arising from relaxed communication or synchronization. In this paper, we connect these two trends, and consider the following question: {\em Can we design machine learning systems that are tolerant to network unreliability during training?} With this motivation, we focus on a theoretical problem of independent interest---given a standard distributed parameter server architecture, if every communication between the worker and the server has a non-zero probability $p$ of being dropped, does there exist an algorithm that still converges, and at what speed? The technical contribution of this paper is a novel theoretical analysis proving that distributed learning over unreliable network can achieve comparable convergence rate to centralized or distributed learning over reliable networks. Further, we prove that the influence of the packet drop rate diminishes with the growth of the number of \textcolor{black}{parameter servers}. We map this theoretical result onto a real-world scenario, training deep neural networks over an unreliable network layer, and conduct network simulation to validate the system improvement by allowing the networks to be unreliable