Split-beam echosounder observations of natural methane seep variability in the northern Gulf of Mexico

A method for positioning and characterizing plumes of bubbles from marine gas seeps using an 18 kHz scientific split-beam echo sounder (SBES) was developed and applied to acoustic observations of plumes of presumed methane gas bubbles originating at approximately 1400 m depth in the northern Gulf of Mexico. A total of 161 plume observations from 27 repeat surveys were grouped by proximity into 35 clusters of gas vent positions on the seafloor. Profiles of acoustic target strength per vertical meter of plume height were calculated with compensation for both the SBES beam pattern and the geometry of plume ensonification. These profiles were used as indicators of the relative fluxes and fates of gas bubbles acoustically observable at 18 kHz and showed significant variability between repeat observations at time intervals of 1 h–7.5 months. Active gas venting was observed during approximately one third of the survey passes at each cluster. While gas flux is not estimated directly in this study owing to lack of bubble size distribution data, repeat surveys at active seep sites showed variations in acoustic response that suggest relative changes in gas flux of up to 1 order of magnitude over time scales of hours. The minimum depths of acoustic plume observations at 18 kHz averaged 875 m and frequently coincided with increased amplitudes of acoustic returns in layers of biological scatterers, suggesting acoustic masking of the gas bubble plumes in these layers. Minimum plume depth estimates were limited by the SBES field of view in only five instances

Acoustic sensing of gas seeps in the deep ocean with split-beam echosounders

When in the form of free gas in the water column, methane seeps emanating from the seabed are strong acoustic targets that are often detectable from surface vessels using echo sounders.In addition to detecting that a seep is present at some location, it is also desirable to characterize the nature of the seep in terms of its morphology and flux rates. Here, we examine how much we can learn about seeps in the deep (\u3e 1000 m) northern Gulf of Mexico using narrow-band split-beam echo sounders operating at fixed frequencies (18 kHz and 38 kHz).Methane seeps in this region are deeper than the methane hydrate stability zone, implying that bubbles of free gas form hydrate rinds that allow them to rise further in the water column than they otherwise would. While this behavior may aid in the classification of gas types in the seep, it is possible that the presence of hydrate rinds may also change the acoustic response of the bubbles and thereby make flux rate estimates more challenging. These and other aspects of seep characterization will be discussed

To chain or not to chain trade-weighted exchange rate indexes

With the advent of chain calculations for the U.S. national income and product accounts, it seems reasonable to contemplate using the chain approach for other indexes, such as trade-weighted exchange rates (TWEXs). A fundamental criticism of measuring the growth of gross domestic product by a fixed-base-year method is that the estimates are highly sensitive, especially when the economy?s structure is changing dramatically, to the arbitrary choice of the base year. Such a criticism can be levied against TWEXs. In fact, even TWEXs constructed using a Paasche index rather than a Laspeyres index have problems related to base periods. We examine theoretically and empirically the use ofa chain TWEX in relation to two well-known TWEX indexes: the Federal Reserve Bank of Atlanta index, which uses a Laspeyres index, and the Federal Reserve Bank of Dallas index, which uses a Paasche index. The choice of base year alters the behavior ofthe dollar in these two indexes. We contrast this result with the behavior of the dollar in comparable chain TWEXs, where the base year sensitivity is absent. Our results indicate that developers of TWEXs, as well as those revising TWEXs, should consider a chain approach. Furthermore, users need to be aware of the sensitivity of TWEXs to changes in either the base period for trade weights or the reference base period for exchange ratesForeign exchange rates ; Dollar, American

Acoustic and optical observations of methane gas seeps in the Gulf of Mexico

In 2011 and 2012, measurements of acoustic backscatter from natural methane seeps were made in the northern Gulf of Mexico in water depths between 1000-2000 m. The measurementswere made using a calibrated 18 kHz echo sounder with an 11 degree beamwidth in order to estimate the depth-dependent target strength (TS). The TS data indicate a wide variation in the rate of gas seepage from the seafloor. Several of these seeps were revisited with a remotely operated vehicle in order to optically assess the bubble size distribution and to estimate the rate at which gas bubbles were exiting the seafloor. The optical data show bubble sizes between 1-10 mm radius, and similar rates of gas seepage ranging from a few bubbles per second to several tens of bubbles per second. Together, these data help to suggest the requirements for acoustically estimating gas flux from the seafloor over large regions

Acoustic estimates of methane gas flux from the seabed in a 6000 km2 region in the Northern Gulf of Mexico

Seeps of free methane gas escaping the seabed can be found throughout the ocean basins. To understand the role of methane gas seeps in the global carbon cycle—including both gas added to the atmosphere and that which is dissolved and potentially oxidized in the ocean volume—it is important to quantify the amount of methane escaping the seabed. Few large-scale mapping projects of natural methane seeps have been undertaken, however, and even among these, quantitative estimates of flux are rare. Here we use acoustic mapping techniques to survey 357 natural methane seeps in a large region (6000 km2) of the northern Gulf of Mexico and outline a general approach for methane seep mapping using a combination of multibeam and split-beam echo sounders. Using additional measurements collected with a remotely operated vehicle (ROV) together with the acoustic mapping results, we estimate the total gas flux within the 6000 km2 region to be between 0.0013 and 0.16 Tg/yr, or between 0.003 and 0.3% of the current estimates for global seabed methane seepage rates

Minimally Invasive Markers of Stress and Production Parameters in Dairy Cows before and after the Installation of a Voluntary Milking System

Automatic milking systems (AMS) are a low-labour alternative to conventional parlours, with previous studies demonstrating that cows vary in their ability to cope with the change to AMS. Cortisol expression can be combined with other measures to assess stress: saliva and hair have the advantage of requiring minimally invasive sampling. No work has investigated the long-term impact of introduction of AMS. The aims of the study were to assess short-term and chronic stress associated with a change in milking system by measuring salivary and hair cortisol levels and to assess the impact on health and production parameters. Cows from one farm changing their milking system were recruited to the study and sampled for saliva (n = 10) and hair (n = 12) before and after installation. Cortisol levels were measured using a salivary cortisol enzyme immunoassay kit. Body condition, lameness and milk parameters of the whole herd were regularly assessed. Salivary cortisol showed no diurnal pattern but was affected by lameness and gestation. Non-lame cows showed a reduction in salivary cortisol after AMS introduction (p < 0.001). Hair cortisol levels increased after AMS, but it was unclear if this change was seasonal. Milk yield increased by 13% and somatic cell count reduced by 28%. Body condition score was consistently good, but lameness remained high throughout the study. Production values alone do not represent high welfare. The high lameness and associated cortisol levels suggest that cow stress requires consideration when changing milking systems

Photon counting strategies with low light level CCDs

Low light level charge coupled devices (L3CCDs) have recently been developed, incorporating on-chip gain. They may be operated to give an effective readout noise much less than one electron by implementing an on-chip gain process allowing the detection of individual photons. However, the gain mechanism is stochastic and so introduces significant extra noise into the system. In this paper we examine how best to process the output signal from an L3CCD so as to minimize the contribution of stochastic noise, while still maintaining photometric accuracy. We achieve this by optimising a transfer function which translates the digitised output signal levels from the L3CCD into a value approximating the photon input as closely as possible by applying thresholding techniques. We identify several thresholding strategies and quantify their impact on photon counting accuracy and effective signal-to-noise. We find that it is possible to eliminate the noise introduced by the gain process at the lowest light levels. Reduced improvements are achieved as the light level increases up to about twenty photons per pixel and above this there is negligible improvement. Operating L3CCDs at very high speeds will keep the photon flux low, giving the best improvements in signal-to-noise ratio.Comment: 7 pages, accepted by MNRA

The development of the human aspects of information security questionnaire (HAIS-Q)

The Human Aspects of Information Security Questionnaire (HAIS-Q) is being developed using a hybrid inductive, exploratory approach, for the purpose of evaluating information security threats caused by employees within organisations. This study reports on the conceptual development and pre-testing of the HAIS-Q. Results from 500 Australian employees were then used to examine the reliability of the HAIS-Q, as well as the relationships between knowledge of policy and procedures, attitude towards policy and procedures and behaviour when using a work computer. Results indicate significant, positive relationships between all variables. However, both qualitative and quantitative results indicate the direct influence of knowledge of policy and procedure accounted for far less of the variance in self-reported behaviour than attitude towards policy and procedure. Implications for training and education campaigns and plans for future research to further develop this questionnaire are outlined

A noninvasive method for measuring mammary apoptosis and epithelial cell activation in dairy animals using microparticles extracted from milk

AbstractMilk production from dairy animals has been described in terms of 3 processes: the increase in secretory cell numbers in late pregnancy and early lactation, secretion rate of milk per cell, and the decline in cell numbers as lactation progresses. This latter process is thought to be determined by the level of programmed cell death (apoptosis) found in the animal. Until now, apoptosis has been measured by taking udder biopsies, using magnetic resonance imaging scans, or using animals postmortem. This paper describes an alternative, noninvasive method for estimating apoptosis by measuring microparticles in milk samples. Microparticles are the product of several processes in dairy animals, including apoptosis. Milk samples from 12 Holstein cows, at or past peak lactation, were collected at 5 monthly samplings. The samples (n=57) were used to measure the number of microparticles and calculate microparticle density for 4 metrics: annexin V positive and merocyanine 540 dye positive, for both and total particles, in both whole milk (WM) and spun milk. Various measures of milk production were also recorded for the 12 cows, including daily milk yield, fat and protein percentage in the milk, somatic cell count, and the days in milk when the samples were taken. A high correlation was found between the 4 WM microparticle densities and days in milk (0.46 to 0.64), and a moderate correlation between WM microparticle densities and daily milk yield (−0.33 to −0.44). No significant relationships were found involving spun milk samples, somatic cell count, or fat and protein percentage. General linear model analyses revealed differences between cows for both level of microparticle density and its rate of change in late lactation. Persistency of lactation was also found to be correlated with the WM microparticle traits (−0.65 to −0.32). As apoptosis is likely to be the major contributor to microparticle numbers in late lactation, this work found a noninvasive method for estimating apoptosis that gave promising results. Further investigation is required to find out the factors affecting microparticle production and how it changes throughout lactation