Reusable, polyethylene glycol-structured microfluidic channel for particle immunoassays

A microfluidic channel made entirely out of polyethylene glycol (PEG), not PEG coating to silicon or polydimethylsiloxane (PDMS) surface, was fabricated and tested for its reusability in particle immunoassays and passive protein fouling, at relatively high target concentrations (1 mg ml-1). The PEG devices were reusable up to ten times while the oxygen-plasma-treated polydimethyl siloxane (PDMS) device could be reused up to four times and plain PDMS were not reusable. Liquid was delivered spontaneously via capillary action and complicated bonding procedure was not necessary. The contact angle analysis revealed that the water contact angle on microchannel surface should be lower than ~60°, which are comparable to those on dried protein films, to be reusable for particle immunoassays and passive protein fouling

The effect of acute intradialytic exercise on cardiovascular responses in hemodialysis patients

In patients with kidney failure requiring hemodialysis (HD) treatment, intradialytic exercise (IDEX, exercise during HD treatment) has been advocated for its feasibility and effectiveness for improving important health outcomes. However, IDEX as an adjunct therapeutic strategy is infrequently implemented, in part due to potential risks of IDEX. The purpose of this study was to evaluate the safety of IDEX by examining its effect on intradialytic cardiovascular (CV) hemodynamics. Intradialytic changes in brachial, aortic and cardiac hemodynamics and autonomic function were examined during a normal HD session without exercise, or when 30-minutes of cycling exercise was performed during the 1st- or 3rd-hour into HD in 12 HD patients. IDEX performed during either the 1st- or 3rd- hour does not appear to exacerbate hemodynamic instability during HD. While there were transient increases in stroke volume, cardiac output and heart rate during IDEX, the intradialytic changes in brachial and aortic blood pressure (BP) parameters, cardiac hemodynamics and autonomic function were similar on days with and without IDEX. This null effect of IDEX on hemodynamic parameters during HD was demonstrated regardless of the timing of exercise and patients’ underlying CV characteristics. Patient hydration status was correlated with the magnitude of BP drop and autonomic dysfunction, and increasing sympathetic activity was also correlated with drops in BP during HD. These results indicate that IDEX does not exacerbate hemodynamic instability during HD regardless of hydration status and the timing of the exercise. We also observed the potential roles of overhydration and autonomic dysfunction on hemodynamic regulation during HD. These results should help to improve our understanding regarding the CV safety of IDEX

Deficits of case marker processing in persons with mild cognitive impairment

The purpose of the current study was to investigate whether persons with mild cognitive impairment (MCI) showed deficits in processing case markers compared to normal elderly adults (NEA). Results revealed that individuals with MCI presented significantly lower accuracy than the NEA group on a case marker processing (CMP) task. Both groups showed greater difficulties in the passive sentences than sentences with the transitive verbs. The current results suggested that individuals with early stage of dementia started presenting deficits in case marker processing compared to the control group

Chemically Exfoliated Graphene Nanosheets for Flexible Electrode Applications

Graphene oxide (GO), produced by oxidation of graphite powder and exfoliation, is intensively utilized in electrodes, templates for hybrid materials, interfacial modifiers, three-dimensional structures, and so on, with its performance as an electrode material being determined by its chemical and structural states. This chapter describes the fabrication method of GO nanosheets from graphite oxide powder and their stable dispersion after reduction and applications in devices. Rheologically driven exfoliation and unusual acoustic cavitation methods were applied to produce large and less defective GO nanosheets. As a dispersion strategy of reduced GO (RGO) in solution, TiO2 precursor, cation-π interaction, silanol groups were introduced. Moreover, supramolecular chemistry, for example, quadruple hydrogen bonding moieties, was applied to solve the dispersion of highly concentrated RGO pastes. As potential applications of GO and RGO, we described GO as a p-type dopant and interfacial modifier as well as energy storage electrodes, IR sensors, and emitters. The judicious use of chemically exfoliated graphene can open new applications as a flexible electrode

Fermentation characteristics of Korean pear (Pyrus pyrifolia Nakai) puree by the Leuconostoc mesenteroides 51-3 strain isolated from Kimchi

A lactic acid bacterial strain showing fast growth and high acid production when cultured in Korean pear puree was isolated from Kimchi. This strain was analyzed by using the API 50 CHL kit and 16S rRNA sequencing and was thus identified as Leuconostoc mesenteroides 51-3. Korean pear puree was fermented with the L. mesenteroides 51-3 strain at 30°C for 12 h. The changes in pH, titratable acidity and viable cell count during fermentation were investigated. The pH and titratable acidity of the pear puree were 4.06 and 0.66%, respectively, after 12 h of fermentation. The viable cell count of L. mesenteroides 51-3 rapidly increased to 3.7 × 109 CFU/g after 12 h of cultivation. The content of lactic acid and acetic acid was determined to be 0.138 and 0.162%, respectively, after 12 h of fermentation. When the fermented pear puree was stored at 4°C, the pH, titratable acidity and viable cell count remained fairly constant for 14 days.Keywords: Fermentation, Korean pear puree, Leuconostoc mesenteroides.African Journal of Biotechnology Vol. 9(35), pp. 5735-5738, 30 August, 201

Malakoplakia of the Kidney Extending to the Descending Colon in a Patient with Secondary Adrenal Insufficiency: A Case Report

Malakoplakia is an uncommon but distinctive type of chronic granulomatous inflammation that occurs most commonly in the genitourinary tract, especially the urinary bladder. Most patients have associated conditions characterized by some degree of immunosuppression, as seen in solid-organ transplants, autoimmune diseases requiring steroid use, chemotherapy, chronic systemic diseases, alcohol abuse and poorly controlled diabetes. We report an unusual case of the renal malakoplakia that involved the perirenal space, extending to the descending colon in a 65-year-old Korean woman with secondary adrenal insufficiency and diabetes mellitus

新規免疫測定素子Quenchbodyに関する基礎的検討

学位の種別:課程博士University of Tokyo(東京大学