Seamless monolithic three-dimensional integration of single-crystalline films by growth

The demand for the three-dimensional (3D) integration of electronic components is on a steady rise. The through-silicon-via (TSV) technique emerges as the only viable method for integrating single-crystalline device components in a 3D format, despite encountering significant processing challenges. While monolithic 3D (M3D) integration schemes show promise, the seamless connection of single-crystalline semiconductors without intervening wafers has yet to be demonstrated. This challenge arises from the inherent difficulty of growing single crystals on amorphous or polycrystalline surfaces post the back-end-of-the-line process at low temperatures to preserve the underlying circuitry. Consequently, a practical growth-based solution for M3D of single crystals remains elusive. Here, we present a method for growing single-crystalline channel materials, specifically composed of transition metal dichalcogenides, on amorphous and polycrystalline surfaces at temperatures lower than 400 {\deg}C. Building on this developed technique, we demonstrate the seamless monolithic integration of vertical single-crystalline logic transistor arrays. This accomplishment leads to the development of unprecedented vertical CMOS arrays, thereby constructing vertical inverters. Ultimately, this achievement sets the stage to pave the way for M3D integration of various electronic and optoelectronic hardware in the form of single crystals

Targeting metastatic breast cancer with peptide epitopes derived from autocatalytic loop of Prss14/ST14 membrane serine protease and with monoclonal antibodies

Background In order to develop a new immunotherapeutic agent targeting metastatic breast cancers, we chose to utilize autocatalytic feature of the membrane serine protease Prss14/ST14, a specific prognosis marker for ER negative breast cancer as a target molecule. Methods The study was conducted using three mouse breast cancer models, 4 T1 and E0771 mouse breast cancer cells into their syngeneic hosts, and an MMTV-PyMT transgenic mouse strain was used. Prss14/ST14 knockdown cells were used to test function in tumor growth and metastasis, peptides derived from the autocatalytic loop for activation were tested as preventive metastasis vaccine, and monoclonal and humanized antibodies to the same epitope were tested as new therapeutic candidates. ELISA, immunoprecipitation, Immunofluorescent staining, and flow cytometry were used to examine antigen binding. The functions of antibodies were tested in vitro for cell migration and in vivo for tumor growth and metastasis. Results Prss14/ST14 is critically involved in the metastasis of breast cancer and poor survival rather than primary tumor growth in two mouse models. The epitopes derived from the specific autocatalytic loop region of Prss14/ST14, based on structural modeling acted as efficient preventive metastasis vaccines in mice. A new specific monoclonal antibody mAb3F3 generated against the engineered loop structure could reduce cell migration, eliminate metastasis in PyMT mice, and can detect the Prss14/ST14 protein expressed in various human cancer cells. Humanized antibody huAb3F3 maintained the specificity and reduced the migration of human breast cancer cells in vitro. Conclusion Our study demonstrates that Prss14/ST14 is an important target for modulating metastasis. Our newly developed hybridoma mAbs and humanized antibody can be further developed as new promising candidates for the use in diagnosis and in immunotherapy of human metastatic breast cancer.This work is supported in part by the National Research Foundation (NRF) grant funded by the Korea government (MEST) (No. 2013R1A1A2009892 and No. 2017R1A2B4008109) and Inha Univeristy Research Grant awarded to MGK and (No. 2015R1A2A1A15054021) to SHK

High Prevalence of Ceftazidime-Resistant Klebsiella pneumoniae and Increase of Imipenem-Resistant Pseudomonas aeruginosa and Acinetobacter spp. in Korea: a KONSAR Program in 2004

A nationwide antimicrobial resistance surveillance has been conducted since 1997 in Korea. In this study, susceptibility test data generated in 2004 by KONSAR group hospitals were analyzed and compared to those at a commercial laboratory. In hospitals, the rank orders of organisms in 2004 were identical to those in 2003. The most prevalent species was Staphylococcus aureus (20.2%) in hospitals, but Escherichia coli (29.7%) in the commercial laboratory. The proportions of Enterococcus faecium to all isolates of Enterococcus faecalis plus E. faecium were 47.2% in hospitals and 24.9% in the commercial laboratory. The mean resistance rates of significant antimicrobial-organism combinations in hospitals were: oxacillin-resistant S. aureus (68%), oxacillin-resistant (penicillin-nonsusceptible) Streptococcus pneumoniae (68%), vancomycin-resistant E. faecium (25%), cefotaxime-resistant E. coli (14%), ceftazidime- and cefoxitin-resistant Klebsiella pneumoniae (34% and 32%, respectively), and imipenem-resistant Acinetobacter spp. and Pseudomonas aeruginosa (17% and 24%, respectively). In conclusion, oxacillin-resistant staphylococci, expanded-spectrum cephalosporin-resistant K. pneumoniae, and imipenem-resistant Acinetobacter spp. and P. aeruginosa were prevalent in 2004. Increasing trends were observed for vancomycin-resistant E. faecium, cefoxitin-resistant E. coli and K. pneumoniae, and imipenem-resistant Acinetobacter spp. and P. aeruginosa. Certain antimicrobial-organism combinations were also prevalent among the commercial laboratory-tested strains

Second-Look Arthroscopic Findings and Clinical Outcomes After Management of Intra-Articular Lesions in Acute Ankle Fractures with Arthroscopy

Category: Ankle, Arthroscopy, Trauma Introduction/Purpose: Anatomically successful surgical reduction of ankle fractures does not always result in a clinically favorable outcome. The purpose of the present study was to compare initial and second look arthroscopic finding of acute ankle fracture and to evaluate clinical outcomes. Methods: A total of 39 patients (40 ankles, 20 male, 20 female) who underwent surgery for ankle fracture between March 2009 and August 2016 were retrospectively reviewed. All patients gave consent to the exploratory arthroscopic surgery for the removal of internal fixation devices placed in the initial surgery. Intra-articular lesions (osteochondral lesion, loose body, injury of ligaments and fibrosis) were evaluated via ankle arthroscopy. Arthroscopic finding of osteochondral lesion were classified using the Ferkel and Cheng staging system, and cartilage repair was assessed using the international Cartilage Repar System (ICRS). Clinicial outcomes were evaluated using the American Orthopaedic Foot and Ankle Society (AOFAS) ankle-hindfoot scale and Visual Analogue Scale (VAS) Results: Chondral lesions were found in 26 ankles (65%) with initial arthroscopic finding of acute ankle fracture. Newly discovered chondral lesions in second-look arthroscopy was 15 cases. Accoding to the Ferkel and Cheng staging at second-look arthroscopy, 4 of 23 ankles with chondral lesions of talus was getting worse (more than stage D). In terms of ICRS overall repair grades, 4 ankle (15%) were abnormal (grade III). Diffuse synovitis and arthrofibrosis were found in 12 and 7 ankles respectively in second-look arthroscopy. Correlation were found between AOFAS scores, VAS and intra-articular lesions with second-look arthroscopy. Conclusion: Second-look arthroscopic examination combined with treatment of intra-articular lesion arising from ankle fracture surgery may consider to improve clinical outcomes

Suture-Tape Augmentation for Chronic Ankle Instability

Category: Ankle Introduction/Purpose: This retrospective study was performed to evaluate the clinical outcomes of internal brace technique using suture tape for chronic ankle instability. Methods: twenty-three patients were followed for more than 2 years after suture tape augmentation for lateral ankle instability. Twenty five patients were followed for more than 2 years after modified Brostrom procedure. The clinical results after using two techniques were evaluated and compared using Foot and Ankle Outcome Score (FAOS), American Orthopaedic Foot and Ankle Society (AOFAS) score, Olarud and Molander score, Hamilton score and Visual Analogue Scale (VAS). Anterior talar translation and talar tilt angle were obtained from stress radiographs to evaluate the mechanical ankle stability. Results: There was no significant difference in VAS at final follow-up (P < .325). There was no significant difference in FAOS at final follow-up (P < .242). There was no significant difference in AOFAS score at final follow-up (P < .418). There was no significant difference in Olarud and Molander score at final follow-up (P < .244). According to the Hamilton score, 14 cases (93%) in internal brace group and 23 cases (92%) achieved satisfactory functional results. Talar tilt angle and anterior talar translation had improved to an average of 2.2 degrees and 4.4 mm in internal brace group and 2.7 degrees and 5.4 mm in modified Brostrom group, respectively, at final follow-up, and there was no significant difference (P < .128, < .226, respectively). There were no complications such as skin irritation and wound infection. Conclusion: Suture tape augmentation has shown the potential for stabilizing the lateral ankle ligmament complex as an alternative to direct repair. Functional, objective, and subjective outcomes were excellent in all cases and no complications were seen postoperatively. To confirm the above further, it is appropriate to conduct a larger study

Conceptual Study for Tissue-Regenerative Biodegradable Magnesium Implant Integrated with Nitric Oxide-Releasing Nanofibers

The excessive initial corrosion rate of Mg is a critical limitation in the clinical application of biodegradable Mg implants because the device loses its fixation strength before the fractured bone heals. This study suggests a new approach to overcome this hurdle by accelerating tissue regeneration instead of delaying the implant biodegradation. As angiogenesis is an essential process in early bone regeneration, a Mg implant coated with electrospun nanofibers containing nitric oxide (NO), which physiologically promotes angiogenesis, is designed. The integrated device enables adjustable amounts of NO to be stored on the NO donor-conjugated nanofiber coating, stably delivered, and released to the fractured bone tissue near the implanted sites. An in vitro corrosion test reveals no adverse effect of the released NO on the corrosion behavior of the Mg implant. Simultaneously, the optimal concentration level of NO released from the implant significantly enhances tube network formation of human umbilical vein endothelial cells without any cytotoxicity problem. This indicates that angiogenesis can be accelerated by combining NO-releasing nanofibers with a Mg implant. With its proven feasibility, the proposed approach could be a novel solution for the initial stability problem of biodegradable Mg implants, leading to successful bone fixation

A metal-ion-chelating organogel electrolyte for Le Chatelier depression of Mn3+ disproportionation of lithium manganese oxide spinel

We present a metal-ion-chelating organogel electrolyte, thermally gelated within cells, to solve the problems triggered by metal dissolution from cathodes of lithium ion batteries. The organogel significantly improved the capacity retention of lithium manganese oxide spinel during cycling. The organogel mitigated metal deposition on anodes by capturing metal ions (anode protection). Interestingly, the organogel inhibited metal dissolution by keeping dissolved metal ions highly concentrated around the cathode surface (cathode protection by Le Chatelier&apos;s principle)