Improving Earth Science Data Literacy

The improvement of scientific literacy across the public can take on a myriad of forms and activities. The vast archives of NASAs Earth science data, as one example, reflect the agencys ongoing commitment to producing the highest quality data, services and tools intended for enabling the public to understand the complexity of Earth systems. The focus of this presentation is to explore and reveal the specific strategies the NASA Earth Science Data and Information System (ESDIS) Project uses to support our very large and diverse user communities access to and understanding of these science data and services. The diversity of our subject matter, spanning the whole Earth science domain, is mirrored by our user communities which are equally as broad in their needs and abilities. ESDIS and the supporting Communication Team leverages an array of internet-based tools and communication strategies to both understand actual user needs and develop optimum pathways for improving our users knowledge and understanding of the data. We will focus our limited time on a few of the high-impact activities including Webinars, or video-based, on-line, interactive discussions and presentations that since their introduction in 2013 have become a staple for user-instructor interaction via the internet. Here we discuss the types of content we produce and many lessons learned on how best to use this medium for improving user literacy on the subject at hand. In addition, we include discussion of our approach to using various social media platforms and tools to bolster the awareness of our users for data and services that are likely of interest. The ESDIS project remains active in various social media campaigns where our particular blend of science, data and tools engages new users curious of how to get their science or application started using NASA-available data. We anticipate that these examples may prove to be novel in their application to improving science literacy and skill development among the public

Pluralismo vivo: lived religious pluralism and interfaith dialogue in Rome

This ethnography of interreligious dialogue in Rome is concerned with how interfaith encounters and social transformation are dialectically constructed and enacted. The network of Roman interfaith organizations is placed in a Durkheimian framework as a moral community with distinct rituals and sacred objects, referred to as the "interfaith society." The interfaith society described here is distinctly shaped by its location in Rome: the neighboring Vatican, engrained cultural Catholicism, and-through global migratory patterns distinct to the late 20th century-the inundation of non-Catholic religions into Italy. This research analyzed the differences that exist between elite institutional events and informal grassroots (di base) gatherings, noting the way third sector nonprofits form a "hinge" between the two. In-depth examination of the publishing cooperative and program office Confronti shows the evolution of Catholic ecumenical efforts into today's interfaith society. It also shows the value of creative dialogue as a form of interfaith engagement. This exploration is based upon interviews with 52 participants across these settings, participant-observation of interfaith practices, and interviews with 17 Romans who do not practice dialogue. Interfaith encounters and interviews with 25 dialoguers in Israel and Palestine illustrate the difference geographical and sociopolitical context can make in the practice of dialogue, and demonstrate that dialogue is framed in both settings as a method to disrupt historical patterns of stereotyping and objectification. This study finds that interfaith dialogue can best be understood by examining its processes and asking what they mean for participants, rather than looking for "metrics." Encounters across religious difference are found to require intention, leadership, and repetition in order to establish a "safe haven." Participants speak of their goals in terms of "humanizing" the other and striving for "mutual recognition." Each of these discursive goals is explored through the narrative data gathered. They are found to be best understood not by measurement of their "success," but as shared sacred values that bind together the interfaith society. The repeated, communal invocation of these sacred values signifies to the members of the community that they belong to the collective, solidifying also awareness of who is not in their group

Something Old, Something New: How Chapbooks and Crowdfunding can Reduce Financial Risk for Small Publishers

Small publishing houses often follow lean and nimble business models, yet the financial risk inherent in publishing for niche readerships can be a barrier for such entrepreneurial practices. Exploring the dual conditions of a less expensive and more eco-friendly format of a chapbook instead of an initial hardcover illustrated book, while determining pre-order interest through a crowdfunding platform, this paper looks at a process to reduce financial risk for small publishers who can’t necessarily do accurate comparative analysis for their sales projections. Common terminology is defined, supplemented by a literature review and a detailed ethnographic approach, including a case study of the author’s crowdfunded chapbook project. The case study includes data on social media advertising and other marketing activities. This paper stands out from existing research in terms of the extent of other publishers interviewed, data collected and the analysis of the marketing information, as well as the children’s picture book and chapbook history that is explored. This case study also occurred during the 2020 pandemic, limiting all marketing activities to online

Purification of A-Raf and structural studies of mannitol dehydrogenase

The work herein describes the research of two separate projects: the purification of A-Raf and the crystallization and X-ray diffraction of Thermotoga maritima mannitol dehydrogenase (TmMtDH). A-Raf is a one of three Raf isoforms of serine/threonine kinases involved in the mitogen-activated protein kinase (MAPK) pathway, a cell proliferation pathway that has been associated with many cancers. In addition, only the A-Raf isoform can uniquely bind to the regulatory subunit of phosphatidylinositol-3-kinase (PI3K), which is part of the Akt/PI3K pathway and is another important signaling molecule deregulated in human cancers. Therefore, the main focus of this study was to purify and crystallize this protein in order to characterize what makes A-Raf structurally unique from the other two Raf isoforms. Several portions of A-Raf were purified throughout this study, but most research concentrated on the conserved region 2 and 3 (CR2 and CR3) domains of A-Raf and the full-length protein. The CR2/CR3 domains and full-length A-Raf were purified by affinity chromatography on a glutathione Sepharose column and column fractions were analyzed by SDS-PAGE. Two different bands measuring approximately 75 kDa and 66 kDa resolved on the SDS-PAGE gel of full-length A-Raf while three bands measuring approximately 75 kDa, 66 kDa and 45 kDa resolved on the SDS-PAGE gel of the CR2/CR3 domains of A-Raf. The CR2/CR3 domains and full-length A-Raf were also extensively studied by mass spectrometry but results were inconclusive. Western blot analysis was also performed on the CR2/CR3 domains and full-length A-Raf. Results indicated that multiple bands were present and that degradation of the protein had taken place. A-Raf was thus deemed unsuitable for crystallization trials and the project was terminated.Mannitol is an acyclic polyalcohol and is used commercially for several purposes including acting as an osmoregulatory compound in several pharmaceuticals and as an artificial sweetener in products targeted for diabetic patients. Commercially, mannitol is produced by the hydrogenation of 50% fructose/50% glucose syrup at high temperatures. However, the product of this process yields an excess of sorbitol and therefore the mannitol requires further purification. Mannitol dehydrogenase catalyzes the conversion of D-fructose to D-mannitol and has therefore been targeted for studies to produce a commercial mannitol bioreactor. The aims of this study included crystallization of the hyperthermophilic Thermotoga maritima (TmMtDH) mannitol dehydrogenase and subsequent X-ray diffraction and structure analysis. Dr. Claire Vieille at Michigan State University provided purified protein for crystallization trials. Two conditions produced diffraction quality crystals of TmMtDH. Condition 1 crystals grew in a solution containing 30% 2-methyl-2,4-pentanediol (MPD) plus 0.1 M HEPES-Na at pH 7.5. Condition 2 crystals grew in a solution containing 15-20% (w/v) polyethylene glycol (PEG) 4000 or 8000 plus 0.1 M sodium citrate at pH 4, 0.2 M sodium bromide and 10% glycerol. Crystals were flash cooled in liquid nitrogen and diffracted on the in-house diffractometer at the Saskatchewan Structural Sciences Center and at beamline 08ID-1 at the Canadian Light Source. Data were collected to 3.3 Å for the crystal that grew in condition 1 but the structure could not be solved before the completion of this project. The space group of the condition 1 crystal was P212121 with unit cell dimensions a = 83.43 Å, b = 120.61 Å, c = 145.76 Å

Primers, Commentaries, and Kanbun Literacy in Japanese Literary Culture, 950-1250CE

This project seeks a new perspective on issues of literacy, literary language, and cultural contact in the literature of premodern Japan by examining the primers used to study Chinese-style literature in the Heian and early medieval periods (c. 900-1250CE). Much of Heian literary production was centered on kanbun: "Chinese-style" writing that resembled classical Chinese and mobilized allusive connections to classical Chinese texts, but was usually read based on classical Japanese vocabulary and syntax. The knowledge gleaned from introductory kanbun education forms an important and little-researched common thread linking readers and writers from a wide range of backgrounds - from male and female courtiers to specialized university scholars to medieval monks and warriors eager to appropriate court culture. While tracing the roles of commonly-studied kanbun primers and commentaries in shaping Heian literary culture and its medieval reception, I consider key aspects of premodern Japan literacy - from the art of kundoku ("gloss-reading") to the systems of knowledge involved in textual commentary and the adaptation of kanbun material. Examining the educational foundations of premodern Japanese literary culture demonstrates that kanbun and other literary styles functioned as closely entangled modes of literacy rather than as native and foreign languages, and that certain elements of classical Chinese knowledge formed a valued set of raw material for literary creativity. Chapter 1 outlines the diversity of premodern Japanese literacy and the key primers and encyclopedic reference works involved in kanbun education. Chapter 2 focuses on a primer for learning written characters, the Thousand Character Classic (Qian zi wen), discussing its varied reception in the contexts of calligraphy practice, oral recitation, and commentarial authority and offering translations from the tongue-in-cheek literary showpiece Thousand Character Classic Continued (Zoku Senjimon, 1132). In Chapter 3, I examine the role of kanbun knowledge in Sei Shonagon's Pillow Book (Makura no soshi, early 11th century), which foregrounds the social and creative roles of introductory kanbun material as a vocabulary of conversational quotation among both men and women. Chapter 4 turns to Condensed Meaning of the New Ballads (Shin gafu ryakui, 1172), a ground-breaking anecdotal commentary on Bai Juyi's poetry, to discuss the way that kanbun texts were interpreted and reinvented through commentary. Chapter 5 discusses an innovative poetic adaptation of a kanbun primer, Waka Poems on the Child's Treasury (Mogyu waka, 1204), which makes use of poetic topics and historical anecdotes as effective ways of organizing kanbun knowledge and also suggest the potential for introductory education to spark literary creativity across genre boundaries. I conclude with a brief look at the relevance of premodern Japanese kanbun education for broader questions about literary language and for comparisons involving other transregional classical languages like Latin. By illustrating the processes by which elements of Chinese literary culture were adopted and adapted throughout East Asia, this project provides fertile ground for exploring issues of literacy and cultural interaction that underlie all forms of literature

Responding to Requests for Assisted Reproductive Technology Intervention Involving Women Who Cannot Give Consent

One of the plots of the Canadian science fiction thriller Orphan Black involves a scheme to create dozens of siblings by harvesting the eggs of one woman, fertilizing them with the sperm of a single man, and implanting them for gestation in dozens of apparently willing surrogates. The casualness of the procedure speaks to how comfortable we have all become with reproduction by technology. Yet there are still aspects of this process that remain outside the normative boundaries of most of our worldviews. This article considers recent advances in assisted reproductive technology (ART) that can result in a viable, fertilized embryo even when the mother is herself either permanently unconscious from a severe injury or has actually lost all brain function and therefore meets the legal criteria for brain death. It reviews these advances and applies them to four scenarios, or vignettes, that represent different concerns about the prospective mother’s intent to reproduce before losing her ability to give consent