Natural antisense transcripts with coding capacity in Arabidopsis may have a regulatory role that is not linked to double-stranded RNA degradation

BACKGROUND: Overlapping transcripts in antisense orientation have the potential to form double-stranded RNA (dsRNA), a substrate for a number of different RNA-modification pathways. One prominent route for dsRNA is its breakdown by Dicer enzyme complexes into small RNAs, a pathway that is widely exploited by RNA interference technology to inactivate defined genes in transgenic lines. The significance of this pathway for endogenous gene regulation remains unclear. RESULTS: We have examined transcription data for overlapping gene pairs in Arabidopsis thaliana. On the basis of an analysis of transcripts with coding regions, we find the majority of overlapping gene pairs to be convergently overlapping pairs (COPs), with the potential for dsRNA formation. In all tissues, COP transcripts are present at a higher frequency compared to the overall gene pool. The probability that both the sense and antisense copy of a COP are co-transcribed matches the theoretical value for coexpression under the assumption that the expression of one partner does not affect the expression of the other. Among COPs, we observe an over-representation of spliced (intron-containing) genes (90%) and of genes with alternatively spliced transcripts. For loci where antisense transcripts overlap with sense transcript introns, we also find a significant bias in favor of alternative splicing and variation of polyadenylation. CONCLUSION: The results argue against a predominant RNA degradation effect induced by dsRNA formation. Instead, our data support alternative roles for dsRNAs. They suggest that at least for a subgroup of COPs, antisense expression may induce alternative splicing or polyadenylation

Vagus nerve stimulation paired with tactile training improved sensory function in a chronic stroke patient

Background: Recent studies indicate that vagus nerve stimulation (VNS) paired with rehabilitation can enhance neural plasticity in the primary sensory and motor cortices, improve forelimb function after stroke in animal models and improve motor function in patients with arm weakness after stroke. OBJECTIVE:To gain “first-in-man” experience of VNS paired with tactile training in a patient with severe sensory impairment after stroke. Methods: During the long-term follow-up phase of a clinical trial of VNS paired with motor rehabilitation, a 71-year-old man who had made good motor recovery had ongoing severe sensory loss in his left hand and arm. He received VNS paired with tactile therapy in an attempt to improve his sensory function. During twenty 2-hour sessions, each passive and active tactile event was paired with a 0.5 second burst of 0.8 mA VNS. Sensory function was measured before, halfway through, and after this therapy. Results: The patient did not report any side effects during or following VNS+Tactile therapy. Quantitative measures revealed lasting and clinically meaningful improvements in tactile threshold, proprioception, and stereognosis. After VNS+Tactile therapy, the patient was able to detect tactile stimulation to his affected hand that was eight times less intense, identify the joint position of his fingers in the affected hand three times more often, and identify everyday objects using his affected hand seven times more often, compared to baseline. Conclusions: Sensory function significantly improved in this man following VNS paired with tactile stimulation. This approach merits further study in controlled clinical trials

The angular momentum of a magnetically trapped atomic condensate

For an atomic condensate in an axially symmetric magnetic trap, the sum of the axial components of the orbital angular momentum and the hyperfine spin is conserved. Inside an Ioffe-Pritchard trap (IPT) whose magnetic field (B-field) is not axially symmetric, the difference of the two becomes surprisingly conserved. In this paper we investigate the relationship between the values of the sum/difference angular momentums for an atomic condensate inside a magnetic trap and the associated gauge potential induced by the adiabatic approximation. Our result provides significant new insight into the vorticity of magnetically trapped atomic quantum gases.Comment: 4 pages, 1 figure

What is in those Compact Shelves? Auditing and Quantifying a Government Documents Collection

Background & Purpose Methodology Planning & Resources Survey Instrument & Implementation Limitations Outcomes & Lessons Learne

Presidential Records & Materials

Research using presidential records can be quite complex. Presidential records are mix of public and private materials, and are located in a variety of archival, library, and museum settings. Understanding a little bit about the history of presidential records helps researchers understand where they can find information about the topics they seek to understand or explain. This display provides an overview of key institutions and a timeline of developments. An associated research guide provides direct access to key collections for each U.S. president

Creating a Government Information Network in Utah – Updates from the Regional Coordinator

Introduction & unpack terminology Details about the Regional Collection and access points Details education and outreach Online resources and an activity Government Information Network Let\u27s work together

Gov Docs are Special Too!: Incorporating Historic Government Documents into Instruction and Outreach Programs

Introduction & unpack terminology Details about the Regional FDLP Collection and access points Primary Sources in Government Documents Online Resources Outreach with Primary Sources Government Information Network Let\u27s work together

Rational Approximate Symmetries of KdV Equation

We construct one-parameter deformation of the Dorfman Hamiltonian operator for the Riemann hierarchy using the quasi-Miura transformation from topological field theory. In this way, one can get the approximately rational symmetries of KdV equation and then investigate its bi-Hamiltonian structure.Comment: 14 pages, no figure