Kvalitet vode za piće naselja Žiča (Kraljevo)

The objective of this work is to present some characteristics of water supply and drinking water quality of Žiča village. The results of analysis of of water qualty provided by the Kraljevo water supply system to most Žiča residents as well as the quality of mountain water used by fewer residents. The physicochemical and microbiological analysis of raw and desinfected water from the town’s water supply system showed its health safety. The physicochemical and microbiological analysis of mountain water indicated its poorer quality. The microbiological testing of mountain water confirm presence of Escherichia coli and mesophilic bacteria as indicators of faecal contamination. It si recommended that the whole Žiča village be connected to the water supply network of the Kraljevo town to help reduce the risk of waterborne epidemics.U radu su prikazane neke karakteristike vodosnabdevanja i kvalitet vode za piće naselja Žiča tokom 2014. i 2015. godine. Analiziran je kvalitet vode iz gradskog vodovoda Kraljevo kojom se snabdeva većina stanovnika i kvalitet planinske vode koju koristi manji broj stanovnika Žiče. Analize fizičko-hemijskih i mikrobioloških pokazatelja kvaliteta sirove i dezinfikovane vode gradskog vodovoda Kraljevo potvrđuju da je voda za piće zdravstveno bezbedna. Rezultati fizičko-hemijskih i mikrobioloških analiza planinske vode ukazuju na njen lošiji kvalitet. Uočena je bakterijska kontaminacija planinske vode bakterijom Escherichia coli i mezofilnim bakterijama koje su indikatori fekalnog zagađenja. Preporuka rada je da se kompletno naselje Žiča priključi na gradsku vodovodnu mrežu Kraljeva čime bi se smanjila mogućnost nastanka hidričnih epidemija

Osobine kvaliteta grožđa i vina sorte merlot (Vitis Vinifera L.) i klonova selekcionisanih u Srbiji

The third phase of individual clone selection of Merlot Cultivar was perfomed at Grocka vineyard, Republic of Serbia. The agrobiological traits and technological traits of three selected clones (022, 025 and 029) and variety (standard) were studied in the period 2009-2013. For all studied traits, except for grape yield, there was statistically significant difference among standard and clones. There was no significant difference between clones for any of the studied traits. The selected clones were characterized by good quality grapes and wines. Clone 025 has been found to have a good attributes for the production of high-quality red wines.Izvršena je selekcija trećeg vegetativnog potomstva klonova sorte Merlot u Gročanskom vinogorju, Republika Srbija. Ispitane su agrobiološke i tehnološke karakteristike grožđa i vina tri odabrana klona (022, 025 i 029) i sorte (standarda) u periodu od 2009 do 2013. godine. Standard i klonovi se statistički razlikuju po svim ispitivanim osobinama, osim po prinosu grožđa. Vina klonova se značajno ne razlikuju. Ispitivani klonovi se karakterišu dobrim kvalitetom grožđa i vina. Klon 025 je pokazao dobre odlike za proizvodnju vrhunskih crvenih vina

Ocena ekološkog statusa reke Zapadne Morave

The paper presents the results of basic physico-chemical and microbiological analyzes of the water quality of the middle course of the West Morava River during the year 2016. The quality of river water of most of the profiles corresponded to moderate ecological status, or III class of surface waters. The worst quality is registered in the Stancici profile, located downstream from the influx of wastewater from the city collector Cacak and the mouth of the Cemernica river. Extremely large microbiological pollution in some periods of the year is characterized by water of this profile as unusable for watering and other purposes. The results obtained confirm the justification for the construction of the Wastewater Treatment System from the city collector Cacak.U radu su prikazani rezultati analiza osnovnih fizičko-hemijskih i mikrobioloških pokazatelja kvaliteta vode srednjeg toka reke Zapadne Morave obavljenih tokom 2016. godine. Kvalitet vode većine profila je odgovarao umerenom ekološkom statusu, odnosno III klasi površinskih voda. Najlošiji kvalitet je registrovan u profilu Stančići, lociranom nizvodno od upliva otpadnih voda gradskog kolektora Čačka i ušća reke Čemernice. Velika mikrobiološka zagađenja u nekim periodima godine karakterišu vodu ovog profila kao neupotrebljivu za zalivanje i druge namene

Fizičko-hemijska karakterizacija uzoraka vode sa teritorije Rasinskog okruga

The aim of the study was a preliminary examination on water quality (U01-U08) in Rasina area, through the standard physico-chemical parameters: temperature, pH, conductivity, turbidity and total organic matter. All samples were odorless and tasteless. The results for temperature, pH and conductivity were within the values defined by Regulation on the quality of drinking water, except for the pH value of the sample U05. Increased turbidity was observed in the sample U03 (13.5 NTU). On the basis of the results for total organic matter (1.2 to 5.5 mg O2/L) that were within the permissible range, it can be concluded that all the studied samples belong to the first (I) class water quality.Cilj rada bio je preliminarno ispitivanje kvaliteta voda sa područja Rasinskog okruga (U01-U08) određivanjem standardnih fizičko-hemijskih parametara: temperature, pH-vrednosti, provodljivost, mutnoće i ukupne organske materije. Svi uzorci su bili bez mirisa i ukusa. Dobijeni rezultati za temperaturu, pH i provodljivost bili su u okviru vrednosti predviđenih Pravilnikom o kvalitetu vode za piće, osim pH vrednost uzorka U05. Povišena mutnoća zabeležena je u uzorku U03 (13,5 NTU). Na osnovu vrednosti za ukupne organske materije (1,2-5,5 mg O2/L) koje su bile u granicama dozvoljenih može se zaključiti da svi ispitivani uzorci pripadaju vodama prve (I) klase kvaliteta

Antiproliferativna aktivnost glutarimidnih derivata iz baze podataka Nacionalnog instituta za rak, SAD - 3D odnos strukture i aktivnosti nezavisan od poravnavanja molekula

Alignment-free, three dimensional structure-activity relationships (3D QSAR) of the antiproliferative potency of twenty-two glutarimide-containing compounds, taken from National Cancer Institute Developmental therapeutics Program database, toward eight representative human tumour cell lines are reported. The descriptors used in the QSAR study were derived from GRID molecular interaction fields. The obtained models readily detect structural motifs positively or negatively correlated with the potency of the studied compounds toward each cell line. In this way, the pharmacophoric pattern required for high potency of compounds is reported. This pattern can serve as guidance for the design and syntheses of novel congeners, planned to be tested toward human tumour cell lines.U tekstu je opisan odnos strukture i antiproliferativne aktivnosti 22 glutarimidna derivata prema osam reprezentativnih linija humanih tumora. Podaci o strukturi jedinjenja i njihovoj aktivnosti su preuzeti iz baze podataka Nacionalnog Instituta za rak, SAD. Deskriptori, nezavisni od poravnavanja molekula (GRIND-2), korišćeni u proučavanju odnosa strukture i aktivnosti su dobijeni upotrebom programa GRID. Modeli jasno prikazuju strukturne elemente jedinjenja koji se pozitivno ili negativno korelišu sa biološkom aktivnošću. Farmakoforna slika dobijena iz modela će biti korišćena za planiranje novih analoga koji sadrže glutarimidni prsten i za koje se očekuje da će pokazati značajnu antiproliferativnu aktivnost

Ispitivanje odabranih fizičko–hemijskih osobina i sadržaja makro- i mikroelemenata u vodi jezera Gruža

Water is one of the most precious natural resources. The downtrend in the quality of lake water and pollution with heavy metals cause serious threats to the environment in last decades. Particularly vulnerable are the lakes that are located near towns and villages. The aim of this study was to investigate the physicochemical parameters and levels of macro– and microelements in order to determine the water quality of Lake Gruža in the summer period. The results showed that the studied parameters were within the permissible levels, except of concentrations of iron, cobalt and vanadium.Voda predstavlja jedan od najdragocenijih prirodnih resursa. Opadanje kvaliteta jezerskih voda i zagađenje teškim metalima, predstavljaju ozbiljne pretnje za životnu sredinu u poslednjih nekoliko decenija. Posebno su ugrožena jezera koja se nalaze u blizini gradova i naselja. Cilj rada bio je ispitivanje osnovnih fizičko–hemijskih parametara i sadržaja makro– i mikroelemenata kako bi se utvrdio kvalitet vode jezera Gruža u letnjem periodu. Rezultati su pokazali da su ispitivani parametri u vodi bili u okviru dozvoljenih vrednosti, osim sadržaja gvožđa, kobalta i vanadijuma

Osvrt na hemijski sastav vina odabranih klonova sorte merlo

Wine is a complex mixture of natural organic compounds belonging to different structural classes. The aim of this study was to examine the chemical composition of Merlot wines of 11 selected clones obtained during third phase of individual clonal selection performed in the Republic of Serbia. The relative density (0.9956 to 0.9975), alcohol content (10.18 to 11.61 vol. %) and total extract (24.23 to 27.80 gl-1) of the wines of screened clones have indicated their good technological characteristics. In order to select the best clones for the final phase of the clonal selection, the evaluation of their viticultural properties and chemical ingredients will be continued.Vino predstavlja kompleksnu smešu prirodnih organskih jedinjenja različitih struktura. Cilj ovog rada bilo je ispitivanje hemijskog sastava vina 11 klonova sorte Merlo u okviru treće faze individualne klonske selekcije u Republici Srbiji. Dobijeni rezultati za relativinu gustinu (0,9956-0,9975), sadržaj alkohola (10,18-11,61 vol%) i ukupan ekstrakt (24,23-27,80 gl-1) ukazuju na dobre tehnološke karakteristike vina klonova. Radi selekcije najboljih klonova za poslednju fazu klonske selekcije, neophodno je sprovesti dalja ispitivanja kako vinogradarskih osobina, tako i hemijskih komponenti relevantnih grožđa i vina

Sadržaj polifenola u grožđu različitih klonova sorte Cabernet franc selekcionisanih u Srbiji

Polyphenols are a large group of structurally diverse compounds widely represented in plants. To a large extent contribute to the nutritional and organoleptic characteristics of fruits and vegetables. The aim of the study was to determine the contents of a selected flavan-3-ols in grape of Cabernet Franc clones (No. 02, 010 and 012) obtained in the last phase perennial clonal selection of the variety in the Republic of Serbia. The enriched content of catechin, epicatechin, epigallocatechin, gallocatechin gallate and catechin gallate, compared to the standard and the other two clones specifically allocated No. 010.Polifenoli su velika grupa strukturno različitih jednjenja široko zastupljenih u biljkama. U velikoj meri doprinose hranjivosti i organoleptičkim osobinama voća i povrća. Cilj rada bio je određivanje sadržaja odabranih flavan-3-ola u grožđu Cabernet Franc klonova (No.02, 010 i 012) dobijenih u poslednjoj fazi višegodišnje klonske selekcije sorte u Republici Srbiji. Po obogaćenom sadržaju katehina, epigalokatehina, epikatehina, epikatehin galata, i katehin galata u grožđu u odnosu na standard i druga dva klona posebno se izdvaja klon No. 010

Different levels of humoral immunoreactivity to different wheat cultivars gliadin are present in patients with celiac disease and in patients with multiple myeloma

<p>Abstract</p> <p>Background</p> <p>Immunity to food antigens (gliadin, cow's milk proteins) is in the centre of the attention of modern medicine focused on the prevention of diseases, prevention which is based on the use of appropriate restriction diet. Detection of the enhanced levels of the immune reactions to antigen(s) present in food is from this point of view of great importance because there are reports that some of health disturbances, like celiac disease (CD) and some premalignant conditions, like monoclonal gammopathy of undetermined significance (MGUS), were vanished after the appropriate restriction diets.</p> <p>It is well known that gliadin is toxic to small bowel mucosa of relatively small population of genetically predisposed individuals, who under this toxic action develop celiac disease (CD). As the quantity of immunogenic gliadin could vary between different wheat species, the first aim of this work was to determine the percentage of immunogenic gliadin in ten bread wheat cultivars and in three commercially grown durum wheat cultivars. The second part of the study was initiated by results of previous publication, reporting that sera of some of multiple myeloma (MM) patients showed the presence of elevated levels of anti-gliadin IgA, without the enhanced levels of anti-gliadin IgG antibodies, determined with commercial ELISA test. It was designed to assess is it possible to reveal is there any hidden, especially anti-gliadin IgG immunoreactivity, in serum of mentioned group of patients. For this purpose we tested MM patients sera, as well as celiac disease (CD) patients sera for the immunoreaction with the native gliadin isolated from wheat species used for bread and pasta making in corresponding geographic region.</p> <p>Results</p> <p>Gliadin was isolated from wheat flour by two step 60% ehanolic extraction. Its content was determined by commercial R5 Mendez Elisa using PWG gliadin as the standard. Results obtained showed that immunogenic gliadin content varies between 50.4 and 65.4 mg/g in bread wheat cultivars and between 20 and 25.6 mg/g in durum wheat cultivars.</p> <p>Anti-gliadin IgA and IgG immunoreactivity of patients' sera in (IU/ml) was firstly determined by commercial diagnostic Binding Site ELISA test, and then additionally by non-commercial ELISA tests, using standardized ethanol wheat extracts -gliadin as the antigen.</p> <p>In both patients groups IgA immunoreactivity to gliadin from different cultivars was almost homogenous and in correlation with results from commercial test (except for one patient with IgA(λ) myeloma, they were more then five times higher). But, results for IgG immunoreactivity were more frequently inhomogeneous, and especially for few MM patients, they were more then five times higher and did not correlate with results obtained using Binding Site test.</p> <p>Conclusion</p> <p>Results obtained showed different content of immunogenic gliadin epitopes in various species of wheat.</p> <p>They also point for new effort to elucidate is there a need to develop new standard antigen, the representative mixture of gliadin isolated from local wheat species used for bread production in corresponding geographic region for ELISA diagnostic tests.</p

Canagliflozin and renal outcomes in type 2 diabetes and nephropathy

BACKGROUND Type 2 diabetes mellitus is the leading cause of kidney failure worldwide, but few effective long-term treatments are available. In cardiovascular trials of inhibitors of sodium–glucose cotransporter 2 (SGLT2), exploratory results have suggested that such drugs may improve renal outcomes in patients with type 2 diabetes. METHODS In this double-blind, randomized trial, we assigned patients with type 2 diabetes and albuminuric chronic kidney disease to receive canagliflozin, an oral SGLT2 inhibitor, at a dose of 100 mg daily or placebo. All the patients had an estimated glomerular filtration rate (GFR) of 30 to &lt;90 ml per minute per 1.73 m2 of body-surface area and albuminuria (ratio of albumin [mg] to creatinine [g], &gt;300 to 5000) and were treated with renin–angiotensin system blockade. The primary outcome was a composite of end-stage kidney disease (dialysis, transplantation, or a sustained estimated GFR of &lt;15 ml per minute per 1.73 m2), a doubling of the serum creatinine level, or death from renal or cardiovascular causes. Prespecified secondary outcomes were tested hierarchically. RESULTS The trial was stopped early after a planned interim analysis on the recommendation of the data and safety monitoring committee. At that time, 4401 patients had undergone randomization, with a median follow-up of 2.62 years. The relative risk of the primary outcome was 30% lower in the canagliflozin group than in the placebo group, with event rates of 43.2 and 61.2 per 1000 patient-years, respectively (hazard ratio, 0.70; 95% confidence interval [CI], 0.59 to 0.82; P=0.00001). The relative risk of the renal-specific composite of end-stage kidney disease, a doubling of the creatinine level, or death from renal causes was lower by 34% (hazard ratio, 0.66; 95% CI, 0.53 to 0.81; P&lt;0.001), and the relative risk of end-stage kidney disease was lower by 32% (hazard ratio, 0.68; 95% CI, 0.54 to 0.86; P=0.002). The canagliflozin group also had a lower risk of cardiovascular death, myocardial infarction, or stroke (hazard ratio, 0.80; 95% CI, 0.67 to 0.95; P=0.01) and hospitalization for heart failure (hazard ratio, 0.61; 95% CI, 0.47 to 0.80; P&lt;0.001). There were no significant differences in rates of amputation or fracture. CONCLUSIONS In patients with type 2 diabetes and kidney disease, the risk of kidney failure and cardiovascular events was lower in the canagliflozin group than in the placebo group at a median follow-up of 2.62 years