Djelovanje regulatora rasta na preživljenje fragmenata hipokotila bundeve

Basic Heller’s medium is not suitable for the growth and development of fragments of pumpkin hypocotyls. On this medium the fragments hardly survive a culture period of one month. The addition of growth regulators (IAA, 2,4-D or kinetin) to Heller’s medium delays the senescence of the tissue and its decay. Moreover, it provokes cell divisions resulting in an increase of fresh and dry weight

Međudržavni skup o školskim jezicima i pravu na višejezično međukulturalno obrazovanje

U Strasbourgu je od 8. do 10. lipnja 2009. održan međudržavni skup o školskim jezicima u organizaciji Odjela jezične politike Europskoga vijeća. Kako je riječ o važnomu skupu, pojedina će se izlaganja prikazati s više pojedinosti kako bi čitatelji dobili što bolji uvid u njega. Ovaj je skup jedan od ishoda projekta posvećenoga jezicima u obrazovanju i za obrazovanje, jednako kao i mrežna Platforma izvora i referenca za višejezično međukulturno obrazovanje, kraticom PIRVIM (eng. PRREPI) o kojoj se na skupu često govorilo. Vjeratno će Platforma utjecati na školske jezike i jezike u obrazovanju općenito slično kao što je i ZEROJ (eng. CEFR, fr. CECR) utjecao na područje poučavanja stranih jezika, ali i na poučavanje (jezicima) općenito

Razvitak abnormalnih embrioida u embriogenom kalusu bundeve (Cucurbita pepo L.)

In addition to normal forms of embryoids in an embryogenic callus of pumpkin, various abnormal forms also appear. They can be characterized, according to their morphology, as monocotyleous, heterocotyleous, tricotyleous, quadricotyleous, syncotyleous, and embryoids with leaf-like cotyledons. Other forms observed include: twins, forms with undeveloped roots, undeveloped hypocotyls, callus formed on the surface of the plantlet, multiple growth and forms of undifferentiated masses. The anomalies described, with the exception of certain differences in the size of the embryoids are not dependent on the presence of a specific growth substances in the nutrient medium

The 17th European carabidologists meeting - continuity in exploring diversity of carabids in relation with current changes in the environment

The 17th European Carabidologists Meeting (17th ECM) was organised in 2015, and for the first time in Croatia. This was a great honour and privilege to join the 12 European countries that have already hosted these distinguished meetings. 48 years have passed since the first meeting was held in Wijster in the Netherlands (1), organised by the Dutch famous ecologist Piet den Boer. The 17th ECM was attended by 112 participants including organisation team members, coming from 21 European countries and form Canada

Influence of meristem culture and virus elimination on phenotypical modifications of grapevine (Vitis vinifera L., cv.Refošk)

Research Not

Regeneracija biljčica u kulturi vegetacijskog vrška vrste Pelargonium zonale hybrid

Shoot tips (0.5 mm high) of Pelargonium zonale hybrid cvs. ’Career’ and ’Fire flash’ were cultured on modified MS medium: macroelements (Murashige and Skoog 1962), microelements (Nitsch and Nitsch 1970) containing (mg 1^1) thiamine.HCl 0.4, m-inositol 200, caseine hydrolysate 1000, IAA 0.5, kinetin 10.0; (g 1-1) sucrose 30, bacto agar 6; pH 5.8. The ’Career’ did not grow on this medium, while the ’Fire flash’ did: 30% of shoots produced an organogenic callus, which was capable of being continuously subcultured. The shoots and plantlets obtained rooted well on a medium containing 0.1 mg 1-1 IAA only, and after a month they were transferred into pots. Callus cultures and in vitro formed plants, examined for Xanthomonas pelargonii and other bacteria, were found to be free of pathogens.Vegetacijske vrškove (veličine 0,5 mm) biljke Pelargonium zonale hybrid 2 sorte: ’Career’ i ’Fire flash’ kultivirali smo na modificiranom MS mediju (Makroelementi po Murashige i Skoogu 1862, mikro- elementi po Nitsch i Nitschu 1970) uz dodane slijedeće tvari: (mg 1-1) tiamina.HCl 0,4; m-inositola 200; hidrolizata kazeina 1000; 0,5 kinetina 10,0 te (g 1-1) saharoze 30 i Difco bakto-agara 6; pH 5,8. Dok sorta ’Career’ nije rasla na mediju ovog sastava, sorta ’Fire flash’ \u27e na 30% eksplantata proizvela dobro rastući organogeni kalus koji se mogao supkultivirati. Izdanci i biljčice regenerirani in vitro dobro su se zakorjenjivali na osnovnom mediju uz dodatak samo 0,1 mg 1-1 IAA i u velikom postotku preživljavali presađivanje u lonce. Testiranje kalusnih kultura i in vitro regeneriranih biljaka na prisutnost bakterija Xanthomonas pelargonii i ostalih bakterija dalo je negativne rezultate