AFLPs: genetic markers for paternity studies in newts (Triturus vulgaris)

DNA-based genetic markers can reveal paternity whenever the direct assignment of fathers to offspring is precluded by multiple matings and internal fertilisation. Microsatellites are the current marker of choice in many behavioural studies, and have revealed important insights into genetic mating systems of European amphibians. However, the number of amphibian species for which the time-consuming designing of locus-specific microsatellite primers was successful is still limited, and the cross-utilisation of existing markers to closely related taxa seems to have a particularly low success rate. Allozymes can infer parentage without a species-specific protocol, but, due to their low degree of polymorphism, in mate choice experiments require the a priori screening of individuals. Dominant markers such as RAPDs successfully identified closely-related amphibian species and their hybrids, but might be less suited to distinguish between closely related individuals with a putatively high frequency of shared bands

The annual number of breeding adults and the effective population size of syntopic newts (Triturus cristatus, T-marmoratus)

Pond-breeding amphibians are deme-structured organisms with a population genetic structure particularly susceptible to demographic threats. We estimated the effective number of breeding adults (N-b) and the effective population size (N-e) of the European urodele amphibians Triturus cristatus (the crested newt) and T. marmoratus (the marbled newt), using temporal shifts in microsatellite allele frequencies. Eight microsatellite loci isolated from a T. cristatus library were used, five of which proved polymorphic in T. marmoratus, albeit with high frequencies of null alleles at two loci. Three ponds in western France were sampled, situated 4-10 kilometres apart and inhabited by both species. Parent-offspring cohort comparisons were used to measure N-b; samples collected at time intervals of nine or 12 years, respectively, were used to measure N-e. The adult population census size (N) was determined by mark-recapture techniques. With one exception, genetic distances (F-ST) between temporal samples were lower than among populations. N-b ranged between 10.6 and 101.8 individuals, N-e ranged between 9.6 and 13.4 individuals. For the pond where both parameters were available, N-b/N (overall range: 0.10-0.19) was marginally larger than N-e/N (overall range: 0.09-0.16), which is reflected in the temporal stability of N. In line with the observed differences in reproductive life-histories between the species, N-b/N ratios for newts were about one order of magnitude higher than for the anuran amphibian Bufo bufo. Despite of the colonization of the study area by T. cristatus only some decades ago, no significant genetic bottleneck could be detected. Our findings give rise to concerns about the long-term demographic viability of amphibian populations in situations typical for European landscapes

Masses and Interactions of q-Fermionic Knots

The q-electroweak theory suggests a description of elementary particles as solitons labelled by the irreducible representations of SU_q(2). Since knots may also be labelled by the irreducible representations of SU_q(2), we study a model of elementary particles based on a one-to-one correspondence between the four families of Fermions (leptons, neutrinos, (-1/3) quarks, (2/3) quarks) and the four simplest knots (trefoils). In this model the three particles of each family are identified with the ground and first two excited states of their common trefoil. Guided by the standard electroweak theory we calculate conditions restricting the masses of the fermions and the interactions between them. In its present form the model predicts a fourth generation of fermions as well as a neutrino spectrum. The same model with q almost equal to 1 is compatible with the Kobayashi-Maskawa matrix. Depending on the test of these predictions, the model may be refined.Comment: 40 pages, 2 figures, latex forma

Linking effective population size dynamics to phenotypic traits in the common toad (Bufo bufo)

The effective size of a population (Ne) determines the retention of neutral genetic variation in isolated populations, and is therefore a key parameter in conservation genetics. However, while our knowledge on the genetic properties of endangered populations has vastly improved in recent decades, rather little is known about the drivers of variation in Ne/Nc (the ratio between Ne and the population census size Nc) within given species. In the present study, we used eight microsatellite markers to genotype 898 adult common toads (Bufo bufo) obtained over five reproductive seasons from a single population (2004–2006 and 2008–2009), and related annual measures of Ne to cohort-specific population attributes. Consistent with the hypothesis that shifts in fitness-related traits can alter reproductive shares, we observed an increase in annual Ne and Ne/Nc ratios in parallel with a decline in body size in both sexes, and in parallel with an increase in body condition in males. The obtained Ne values also corresponded well with parentage inferences across the 6-year study period, which assigned 46.3% of individuals recorded in 2008 and 2009 to at least one putative parent from the 2004–2006 cohorts. Our study highlights a possible causative link between phenotypic traits such as body size and Ne/Nc, which has the potential to influence the amount of genetic erosion through drift. Keywords:Amphibians; Anurans; Genetic compensation; Genetic monitoring; Microsatellite

Skin swabs with FTA® cards as a dry storage source for amphibian DNA

Amphibians are the most endangered group of vertebrates, and conservation measures increasingly rely on information drawn from genetic markers. The present study explores skin swabs with Whatman FTA® cards as a method to retrieve PCR-amplifiable amphibian DNA. Swabs from ten adult great crested newts (Triturus cristatus) were used to compare FTA® card-based protocols with tissue sampling based on toe clips. PCR success rates were measured for seven microsatellite markers and one mtDNA marker (ND4) after 6 months of sample storage. We demonstrate that the merging of eight FTA® card punches from Qiagen-based DNA extraction always led to successful amplifications in at least one replicate, at an overall PCR success rate of 78%. The newly established protocol has the potential for wide application to future DNA-based amphibian studies

Age and growth in a European flagship amphibian : equal performance at agricultural ponds and favourably managed aquatic sites

In human-modified landscapes, little is known about the influence of aquatic habitat types on the demographic structure of residing amphibian populations. In the present paper, we focus on a European flagship urodele species (the great crested newt Triturus cristatus) at the north-western range of its distribution, applying the method of skeletochronology to compare the ages of individuals retrieved from agricultural ponds with individuals retrieved from aquatic sites favourably managed for T. cristatus presence. Median ages ranged between 4.5 and 10.0 years depending on sex and population, and did not differ between the two site categories. Females were on average older than males at both agricultural ponds as well as favourably managed sites. Median ages at sexual maturity (3 years for females and 2 years for males) were 4 years below the most commonly observed age cohort in both sexes, suggesting that young adults regularly forgo reproduction. Mean body size did not differ between agricultural ponds and favourably managed sites. However, the former were characterised by a higher variance in body size, which is possibly linked to more unstable ecological conditions in agricultural settings. Taken together, our findings confirm that under suitable conditions agricultural ponds can harbour sustainable populations, an important finding for the broad-scale conservation management of T. cristatus which does not usually take population demographies into account. Keywords: Demography Great crested newt Skeletochronology Triturus cristatus Urodele

Introduction to the special issue ‘Integrated scenario building in energy transition research

Adaptive governance approaches emphasize the crucial role of the private sector in enabling climate change adaptation. Yet, the participation of local firms is still lacking, and little is known about the conditions potentially influencing firms’ adaptation decisions and mechanisms that might encourage private sector engagement. We address this gap with an empirical analysis of the willingness of manufacturing small- and medium-sized enterprises (SMEs) to participate financially in collective flood adaptation in Ho Chi Minh City (HCMC), a hotspot of future climate change risk. Using scenario-based field experiments, we shed light on internal and external conditions that influence potential investments in collective initiatives and explain what role SMEs can play in flood adaptation. We find that direct impacts of floods, perceived self-responsibility, and strong local ties motivate firms to participate in collective adaptation, whereas government support, sufficient financial resources, and previously implemented flood protection strategies reduce the necessity to act collectively. Here, opportunity costs and the handling of other business risks play a decisive role in investment decisions. This study shows that although private sector engagement appears to be a promising approach, it is not a panacea. Collective initiatives on flood adaptation need formal guidance and should involve local business networks and partnerships to give voice to the needs and capacities of SMEs, but such initiatives should not overstretch firms’ responsibilities

Evolution of sperm morphology in anurans : Insights into the roles of mating system and spawning location

Background: The degree of postcopulatory sexual selection, comprising variable degrees of sperm competition and cryptic female choice, is an important evolutionary force to influence sperm form and function. Here we investigated the effects of mating system and spawning location on the evolution of sperm morphology in 67 species of Chinese anurans. We also examined how relative testes mass as an indicator of the level of sperm competition affected variation in sperm morphology across a subset of 29 species. Results: We found a significant association of mating system and spawning location with sperm morphology. However, when removing the effects of body mass or absolute testes mass for species for which such data were available, this effect became non-significant. Consistent with predictions from sperm competition theory, we found a positive correlation between sperm morphology and relative testes mass after taking phylogeny into account. Conclusions: Our findings suggest that sexual selection in Chinese anurans favors longer sperm when the level of sperm competition is high. Pre-copulatory male-male competition and spawning location, on the other hand, do not affect the evolution of sperm morphology after taking body mass and absolute testes mass into account

Genetic diversity of common toads (Bufo bufo) along the Norwegian coast : disjunct distribution of locally dominant haplotypes

Little is known about the phylogeographic history of amphibian populations along the western Fennoscandinavian coast. In the present study, we focus on the common toad (Bufo bufo) and document the spatial distribution of mitochondrial DNA (cytb) haplotypes at 20 localities along its coastal Norwegian range. Two common haplotypes (out of eight haplotypes in total) were represented by 142 out of the 154 (92%) investigated individuals. However, they were shared at only three localities and clustered at two separate geographic regions each. The most common haplotype (55% of individuals) has previously been found to be abundant across central and eastern Europe, whereas the second most common haplotype (37% of individuals) has so far only been recorded in Sweden. The disjunct distribution of genetic lineages is in line with an assumption that the Norwegian coastline was postglacially colonised both from the south as well as across mountain passes from the east. Our data support previous studies on the phylogeography of Fennoscandinavia that revealed that post-glacial recolonisation patterns led to a pronounced spatial structure of local populations

Habitat requirements and conservation needs of peripheral populations : the case of the great crested newt (Triturus cristatus) in the Scottish Highlands

Edge populations are of conservation importance because of their roles as reservoirs of evolutionary potential and in understanding a given species’ ecological needs. Mainly due to loss of aquatic breeding sites, the great crested newt Triturus cristatus is amongst the fastest declining amphibian species in Europe. Focusing on the north-westerly limit of the T. cristatus range, in the Scottish Highlands, we aimed to characterise habitat requirements and conservation needs of an isolated set of edge populations. We recorded 129 breeding-pond related environmental parameters, and used a variable-selection procedure followed by random forest analysis to build a predictive model for the species’ present occurrence, as well as for population persistence incorporating data on population losses. The most important variables predicting T. cristatus occurrence and persistence were associated with pond quality, pond shore and surrounding terrestrial habitat (especially mixed Pinus sylvestris - Betula woodland), and differed from those identified in the species’ core range. We propose that habitat management and pond creation should focus on the locally most favourable habitat characteristics to improve the conservation status and resilience of populations. This collaborative work, between conservation agencies and scientific researchers, is presented as an illustrative example of linking research, management and conservation