Diagnostic du psoriasis par la stéréo photométrie

International audienc

Non invasive control and scoring of psoriasis severity

International audienc

Gravity-wave effects on tracer gases and stratospheric aerosol concentrations during the 2013 ChArMEx campaign

International audienceCoupled balloon-borne observations of Light Optical Aerosol Counter (LOAC), M10 meteorological global positioning system (GPS) sondes, ozonesondes, and GPS radio occultation data, are examined to identify gravity-wave (GW)-induced fluctuations on tracer gases and on the vertical distribution of stratospheric aerosol concentrations during the 2013 ChArMEx (Chemistry-Aerosol Mediterranean Experiment) campaign. Observations reveal signatures of GWs with short vertical wavelengths less than 4 km in dynamical parameters and tracer constituents, which are also correlated with the presence of thin layers of strong local enhancements of aerosol concentrations in the upper troposphere and the lower stratosphere. In particular, this is evident from a case study above Ile du Levant (43.02° N, 6.46° E) on 26–29 July 2013. Observations show a strong activity of dominant mesoscale inertia GWs with horizontal and vertical wavelengths of 370–510 km and 2–3 km respectively, and periods of 10–13 h propagating southward at altitudes of 13–20 km during 27–28 July. The European Centre for Medium-Range Weather Forecasts (ECMWF) analyses also show evidence of mesoscale inertia GWs with similar horizontal characteristics above the eastern part of France. Ray-tracing experiments indicate the jet-front system as the main source of observed GWs. Using a simplified linear GW theory, synthetic vertical profiles of dynamical parameters with large stratospheric vertical wind maximum oscillations of ±40 mms−1 are produced for the dominant mesoscale GW observed at heights of 13–20 km. Parcel advection method reveals signatures of GWs in the ozone mixing ratio and the tropospheric-specific humidity. Simulated vertical wind perturbations of the dominant GWs and small-scale perturbations of aerosol concentration (aerosol size of 0.2–0.7 µm) are revealed to be in phase in the lower stratosphere. Present results support the importance of vertical wind perturbations in the GW–aerosol relationship. Observed mesoscale GWs induce a strong modulation of the amplitude of tracer gases and the stratospheric aerosol background

Synergistic activity of proinflammatory cytokines on inhibition of KDM expression by NHEK.

<p>NHEK were cultured in the presence or absence of 10/ml of IL-1α, IL-17A, IL-22, OSM and TNFα alone or in combination for 24 h. Quantitative RT-PCR analysis was carried out on total RNA from 4 independent NHEK cultures. mRNA expression levels for cytokeratin 10 (CK10), cytokeratin 1 (CK1), desmoglein 1 (DSG1), desmocollin 1 (DSC1), fatty acid binding protein 5 (FABP5), calmodulin-like skin protein (CLSP), loricrin (LOR) and filaggrin (FLG) were normalized using GAPDH housekeeping gene and expressed as the fold decrease under unstimulated cultures. (A) Comparison of the activity of IL-1α, IL-17A, IL-22, OSM and TNFα alone or in combination (M5) on expression of keratinocyte differentiation markers. (B) Comparison of the activity of mix of 4 cytokines versus mix of 5 cytokines (M5) on expression of keratinocyte differentiation markers. All data are represented as mean and SEM of 4 independent experiments. One-way ANOVA with a Dunnett post-test were used for statistical evaluation and p values were as follows: *p<0.05, **p<0.01, ***p<0.001.</p

Synergistic activity of proinflammatory cytokines on inhibition of KDM expression by Reconstituted Human Epidermis.

<p>RHE have been cultured for 10-water interface using an appropriate differentiation medium and then with or without recombinant IL-1α, IL-17A, IL-22, OSM or TNFα alone or in combination during 24 h for mRNA quantification. Quantitative RT-PCR analysis was carried out and expression levels for KDM were normalized using GAPDH housekeeping gene and expressed as the fold to unstimulated control cultures. Data are mean and SEM of one experiment representative of two. One-way ANOVA with a Dunnett post-test were used for statistical evaluation and p values were as follows: *p<0.05, **p<0.01, ***p<0.001.</p

Activities of proinflammatory cytokines on the differentiation of Reconstituted Human Epidermis.

<p>(A) RHE have been cultured for 10 days at the air-water interface using an appropriate differentiation medium and then with or without recombinant IL-1α, IL-17A, IL-22, OSM or TNFα alone or in combination during 72 h for immunohistological analysis. RHE were fixed, embedded in paraffin and 4 µm vertical sections were stained with Hematoxylin and Eosin (HE) or with anti-CK10, anti-LOR, anti-FLG, anti-IVL or anti-S100A7 mAbs. Results are from one experiment representative of two. (B) RHE have been cultured for 10 days at the air-water interface using an appropriate differentiation medium and then with or without recombinant IL-1α, IL-17A, IL-22, OSM and TNFα (3 ng/ml), with or without JAKs inhibitor (10 µM) during 72 h. RHE were fixed, embedded in paraffin and 4 µm vertical sections were stained with Hematoxylin and Eosin. Results are from one experiment representative of three.</p