The Coherence Problem: Mapping the Theory and Delivery of Infrastructure Resilience Across Concept, Form, Function, and Experienced Value

In this contribution we explore the interface between the functional characteristics of infrastructures as artefacts and social need supplier. Specifically we are concerned with the ways in which infrastructure performance measures are articulated and assessed and whether there are incongruities between the technical and broader, social goals which infrastructure systems are intended to aspire to. Our analysis involves comparing and contrasting system design and performance metrics across the technical — social boundary, generating new insights for those tasked with the design and operation of networked infrastructures. The assessment delivered in the following sections is inherently interdisciplinary and cross-sectoral in nature, bringing thinking from the social and environmental sciences together with contributions from mathematics and engineering to offer a commentary which is relevant to all types of physical infrastructure

Public perceptions of recycled water: a survey of visitors to the London 2012 Olympic Park

The Old Ford Water Recycling Plant, operated by Thames Water, was used to supply non-potable recycled blackwater to some of the venues at the London 2012 Games. In an effort to learn from this experience, Thames Water commissioned a survey of visitors to the Olympic Park during the Games to explore public responses to the water recycling project. Results show a very high level of support for using non-potable recycled blackwater, both in public venues and in homes. Such findings may indicate a growing receptivity towards this technology, and show that Thames Water (and other private water companies) are well placed to encourage and even lead public discussion around the role of water reuse in the future of urban water supplies

Characterization of neurotensin\u27s bipolar effects on nociceptive modulation using SR 142948A and SR 48692, two non-peptide neurotensin receptor antagonists

Results from the current study confirmed that neurotensin has a dose-dependent bipolar effects on nociception within the RVM. Microinjection of low (picomolar) doses of neurotensin into the RVM resulted in a facilitation of nociception and resolved the anti-analgesic properties (tail flick test) of the peptide in models where basal analgesia is established by stressing rats or following PAG administration of opioids (morphine). Whereas microinjection of higher (nanomolar) doses of neurotensin into the RVM produced antinociception. Furthermore, these same pain facilitatory and inhibitory effects of neurotensin were observed following i.c.v. administration.;Direct evaluation and comparisions of the two non-peptide neurotensin receptor antagonists, SR 48692 and SR 142948A, revealed that they differ in their ability to modulate these actions of neurotensin. In contrast to SR 48692, SR 142948A resulted in a complete inhibition of neurotensin-mediated antinociception in a dose-dependent bell-shaped manner. Further discrimination between the two antagonists was demonstrated in their ability to inhibit neurotensin-mediated pain facilitation within the RVM. In contrast to SR 48692, SR 142948A administration into the RVM (1) did not produce a significant antinociceptive effect when administered alone; (2) failed to promote antinociception from microinjection of a low (30 pmol) dose of neurotensin microinjected into the RVM; (3) did not inhibit the anti-analgesic effect of a low (3 pmol) dose of neurotensin administered into the RVM of a stressed rat; (4) microinjection of SR 142948A dose selectively (only a 30 pmol dose) resulted in a potentiation of the antinociceptive response to morphine (6 nmol) administered into the PAG.;Moreover, these direct comparisons demonstrated that the ability of neurotensin to produce its bipolar effects relies on the ability of the peptide to interact with multiple receptors within the RVM. Furthermore, studies performed using levocabastine, a selective antagonist of the NTR2 receptor, demonstrated that the action of neurotensin within the RVM is mediated via receptors or receptor subtypes that do not share the characteristic properties of the NTR2 receptor. Therefore, these studies indicate the existence and involvement of multiple NTR1 receptor subtypes or splice variants in mediating these actions of neurotensin within the RVM

NGC 4314. III. Inflowing Molecular Gas Feeding a Nuclear Ring of Star Formation

NGC 4314 is an early-type barred galaxy containing a nuclear ring of recent star formation. We present CO(1-0) interferometer data of the bar and circumnuclear region with 2.3 x 2.2 arcsec spatial resolution and 13 km/s velocity resolution acquired at the Owens Valley Radio Observatory . These data reveal a clumpy circumnuclear ring of molecular gas. We also find a peak of CO inside the ring within 2 arcsec of the optical center that is not associated with massive star formation. We construct a rotation curve from these CO kinematic data and the mass model of Combes et al. (1992). Using this rotation curve, we have identified the location of orbital resonances in the galaxy. Assuming that the bar ends at corotation, the circumnuclear ring of star formation lies between two Inner Lindblad Resonances, while the nuclear stellar bar ends near the IILR. Deviations from circular motion are detected just beyond the CO and H-alpha ring, where the dust lanes along the leading edge of the bar intersect the nuclear ring. These non-circular motions along the minor axis correspond to radially inward streaming motions at speeds of 20 - 90 km/s and clearly show inflowing gas feeding an ILR ring. There are bright HII regions near the ends of this inflow region, perhaps indicating triggering of star formation by the inflow.Comment: 25 pages, uses aasms.sty. 7 Postscript figures, 12 JPEG figures. Figures may be retrieved from ftp://clyde.as.utexas.edu/pub/N4314COfigs.tar.g

Monomerization of Cytosolic Mature Smac Attenuates Interaction with IAPs and Potentiation of Caspase Activation

The four residues at the amino-terminus of mature Smac/DIABLO are an IAP binding motif (IBM). Upon exit from mitochondria, mature Smac interacts with inhibitor of apoptosis proteins (IAPs), abrogating caspase inhibition. We used the ubiquitin fusion model to express mature Smac in the cytosol. Transiently expressed mature Smac56-239 (called Smac56) and Smac60-239 (called Smac60), which lacks the IBM, interacted with X-linked inhibitor of apoptosis protein (XIAP). However, stable expression produced wild type Smac56 that failed to homodimerize, interact with XIAP, and potentiate caspase activation. Cytosolic Smac60 retained these functions. Cytosolic Smac56 apparently becomes posttranslationally modified at the dimer interface region, which obliterated the epitope for a monoclonal antibody. Cytosolic Smacδ, which has the IBM but lacks amino acids 62–105, homodimerized and weakly interacted with XIAP, but failed to potentiate apoptosis. These findings suggest that the IBM of Smac is a recognition point for a posttranslational modification(s) that blocks homodimerization and IAP interaction, and that amino acids 62–105 are required for the proapoptotic function of Smac

NEW AND UPDATED RECORDS FOR AMPHIBIANS AND REPTILES IN MINNESOTA, USA

Following the publication of the revised edition of “Amphibians and Reptiles in Minnesota” by Moriarty and Hall (2014), we accessioned several new or updated records at the Bell Museum of Natural History (JFBM). Records include digital photographs (accession number preceded by “P”) and audio recordings (accession number preceded by “AUD”). In addition, a subset of these observations were accessioned in www.HerpMapper.org. HerpMapper accession numbers are preceded by “HM” and can be viewed online. Benjamin Lowe verified species determinations. Latitude and longitude coordinates are based on datum WGS 84

Copper Inhibits NMDA Receptor-Independent LTP and Modulates the Paired-Pulse Ratio after LTP in Mouse Hippocampal Slices

Copper misregulation has been implicated in the pathological processes underlying deterioration of learning and memory in Alzheimer's disease and other neurodegenerative disorders. Supporting this, inhibition of long-term potentiation (LTP) by copper (II) has been well established, but the exact mechanism is poorly characterized. It is thought that an interaction between copper and postsynaptic NMDA receptors is a major part of the mechanism; however, in this study, we found that copper (II) inhibited NMDA receptor-independent LTP in the CA3 region of hippocampal slices. In addition, in the CA3 and CA1 regions, copper modulated the paired-pulse ratio (PPR) in an LTP-dependent manner. Combined, this suggests the involvement of a presynaptic mechanism in the modulation of synaptic plasticity by copper. Inhibition of the copper-dependent changes in the PPR with cyclothiazide suggested that this may involve an interaction with the presynaptic AMPA receptors that regulate neurotransmitter release