Behavioral ontogeny in larvae and early juveniles of the giant trevally (Caranx ignobilis) (Pisces: Carangidae)

Behavior of young (8−18 mm SL) giant trevally (Caranx ignobilis), a large coral-reef−associated predator, was observed in the laboratory and the ocean. Size was a better predictor of swimming speed and endurance than was age. Critical speed increased with size from 12 to 40 cm/s at 2.7 cm/s for each mm increase in size. Mean scaled critical speed was 19 body lengths/s and was not size related. Swimming speed in the ocean was 4 to 20 cm/s (about half of critical speed) and varied among areas, but within each area, it increased at 2 cm/s for each mm increase in size. Swimming endurance in the laboratory increased from 5 to 40 km at 5 km for each mm increase in size. Vertical distribution changed ontogenetically: larvae swam shallower, but more variably, and then deeper with growth. Two-thirds of individuals swam directionally with no ontogenetic increase in orientation precision. Larvae swam offshore off open coasts, but not in a bay. In situ observations of C. ignobilis feeding, interacting with pelagic animals, and reacting to reefs are reported. Manus

Desarrollo de la morfología y la natación en las larvas de un pez de arrecifes de coral, grama real, Gramma loreto (Grammatidae: Teleostei)

We used reared larvae of Gramma loreto sized 5.5 to 10.5 mm to study ontogeny of morphology and swim- ming. The larvae are slender to moderate in depth, with laterally compressed bodies, about 28 myomeres, a coiled gut that is not compact, extending to 50% to 60% of body length, an inconspicuous gas bladder, head spination limited to tiny preopercular spines, no pectoral-girdle spination, more spines than soft rays in the dorsal fin, and fins without elongate or early-forming elements. The limited pigment is most prominent along the base of the dorsal and anal fins. The development of critical speed (Ucrit) in G. loreto larvae was similar to that of other warm-water species, but speed ranged from 0.2 to 13.5 cm s-1 (mean 7.3, SE 0.7, body lengths s-1), which is relatively slow. Increase in speed with size was linear at 2.7 cm s-1 per mm increase in size. The actual and relative Ucrit of G. loreto were low, and only the four largest, fastest larvae would have been swimming in an inertial hydrodynamic environment. Therefore, for most of their pelagic larval duration the ability of G. loreto larvae to influence their dispersal by horizontal swimming is likely to be low compared with that of many other warm-water fish species.Hemos utilizado larvas cultivadas de Gramma loreto, 5.5-10.5 mm, para estudiar el desarrollo ontogénico en morfología y natación. Las larvas son delgadas o moderamente gruesas, con cuerpos comprimidos lateralmente, con 28 miómeros, un tubo digestivo en espiral, no compacto y que se extiende hasta el 50-60% de la longitud del cuerpo, con una vejiga natatoria inconspicua, espinas en la cabeza limitadas a diminutas espinas preoperculares, sin espinas en la pectoral, mayor número de espinas que de radios blandos en la aleta dorsal, y aletas sin elementos alargados. La pigmentación es limitada, siendo más prominente a lo largo de la base de las aletas dorsal y anal. El desarrollo de la velocidad crítica (Ucrit) en las larvas de G. loreto fue similar al de otras especies de aguas cálidas, sin embargo la velocidad varió de 0.2 a 13.5 cm s-1 (promedio 7.3, ES 0.7, longitud del cuerpo s-1), lo que es relativamente lento. El incremento de velocidad con la talla fue lineal a 2.7 cm s-1 por mm de incremento en talla. La Ucrit real y la relativa de G. loreto fue baja, y solamente las cuatro larvas más grandes y más rápidas , hubieran nadado en un ambiente hidrodinámico inercial. Por tanto, durante la mayor parte de su periodo larvario pelágico la capacidad de las larvas de G. loreto para influir en su dispersión por la natación horizontal es probablemente baja en comparación con muchas otras especies de peces de aguas cálidas

Tropical larval and juvenile fish critical swimming speed (U-crit) and morphology data

Fish swimming capacity is a key life history trait critical to many aspects of their ecology. U-crit (critical) swimming speeds provide a robust, repeatable relative measure of swimming speed that can serve as a useful surrogate for other measures of swimming performance. Here we collate and make available one the most comprehensive datasets on U-crit swimming abilities of tropical marine fish larvae and pelagic juveniles, most of which are reef associated as adults. The dataset includes U-crit speed measurements for settlement stage fishes across a large range of species and families obtained mostly from field specimens collected in light traps and crest nets; and the development of swimming abilities throughout ontogeny for a range of species using reared larvae. In nearly all instances, the size of the individual was available, and in many cases, data include other morphological measurements (e.g. “propulsive area”) useful for predicting swimming capacity. We hope these data prove useful for further studies of larval swimming performance and other broader syntheses

In situ orientation of fish larvae can vary among regions

Larval coral-reef fishes have good orientation abilities. Through-water orientation of larvae in some species is location-dependent at meso-scales 90% of larvae swam directionally with similar precision and speeds, and with significant among-individual orientation. Yet through-water orientation was easterly at CGBR (72 +/- 30 degrees) and NCRL (87 +/- 20 degrees), and significantly different from NGBR. Over-bottom orientation (i.e. the result of current and larval swimming), measured by GPS at start and end of observing each larva, was weak east-southeasterly at NGBR (116 +/- 40 degrees, p = 0.045), not significantly directional at CGBR, and strongly westerly at NCRL (246 +/- 28 degrees, p = 0.0006), indicating that dispersal of C. atripectoralis is both current-and behaviour-dependent. This is the first report of location-dependent larval fish orientation at a regional scale. This might be an evolutionary response to regional hydrodynamic conditions to limit downstream dispersal

Does fish larval dispersal differ between high and low latitudes?

Author Posting. © The Author(s), 2013. This is the author's version of the work. It is posted here by permission of The Royal Society for personal use, not for redistribution. The definitive version was published in Proceedings of the Royal Society B Biological Sciences 280 (2013): 20130327, doi:10.1098/rspb.2013.0327.Several factors lead to expectations that the scale of larval dispersal and population connectivity of marine animals differs with latitude. We examine this expectation for demersal shorefishes, including relevant mechanisms, assumptions, and evidence. We explore latitudinal differences in: 1) biological (e.g., species composition, spawning mode, pelagic larval duration (PLD)), 2) physical (e.g., water movement, habitat fragmentation), and 3) biophysical factors (primarily temperature, which could strongly affect development, swimming ability, or feeding). Latitudinal differences exist in taxonomic composition, habitat fragmentation, temperature, and larval swimming, and each could influence larval dispersal. Nevertheless, clear evidence for latitudinal differences in larval dispersal at the level of broad faunas is lacking. For example, PLD is strongly influenced by taxon, habitat, and geographic region, but no independent latitudinal trend is present in published PLD values. Any trends in larval dispersal may be obscured by a lack of appropriate information, or use of ‘off the shelf’ information that is biased with regard to the species assemblages in areas of concern. Biases may also be introduced from latitudinal differences in taxa or spawning modes, as well as limited latitudinal sampling. We suggest research to make progress on the question of latitudinal trends in larval dispersal.TK was supported by the Norwegian Research Council through project MENUII #190286. JML was supported by ARC Discovery Grant DP110100695. JEC and RRW were supported by the Partnership for the Interdisciplinary Study of Coastal Oceans, funded by The David and Lucille Packard Foundation and the Gordon and Betty Moore Foundation.2014-03-2

Cerebrospinal fluid biomarker candidates associated with human WNV neuroinvasive disease

During the last decade, the epidemiology of WNV in humans has changed in the southern regions of Europe, with high incidence of West Nile fever (WNF) cases, but also of West Nile neuroinvasive disease (WNND). The lack of human vaccine or specific treatment against WNV infection imparts a pressing need to characterize indicators associated with neurological involvement. By its intimacy with central nervous system (CNS) structures, modifications in the cerebrospinal fluid (CSF) composition could accurately reflect CNS pathological process. Until now, few studies investigated the association between imbalance of CSF elements and severity of WNV infection. The aim of the present study was to apply the iTRAQ technology in order to identify the CSF proteins whose abundances are modified in patients with WNND. Forty-seven proteins were found modified in the CSF of WNND patients as compared to control groups, and most of them are reported for the first time in the context of WNND. On the basis of their known biological functions, several of these proteins were associated with inflammatory response. Among them, Defensin-1 alpha (DEFA1), a protein reported with anti-viral effects, presente

A Test of Highly Optimized Tolerance Reveals Fragile Cell-Cycle Mechanisms Are Molecular Targets in Clinical Cancer Trials

Robustness, a long-recognized property of living systems, allows function in the face of uncertainty while fragility, i.e., extreme sensitivity, can potentially lead to catastrophic failure following seemingly innocuous perturbations. Carlson and Doyle hypothesized that highly-evolved networks, e.g., those involved in cell-cycle regulation, can be resistant to some perturbations while highly sensitive to others. The “robust yet fragile” duality of networks has been termed Highly Optimized Tolerance (HOT) and has been the basis of new lines of inquiry in computational and experimental biology. In this study, we tested the working hypothesis that cell-cycle control architectures obey the HOT paradigm. Three cell-cycle models were analyzed using monte-carlo sensitivity analysis. Overall state sensitivity coefficients, which quantify the robustness or fragility of a given mechanism, were calculated using a monte-carlo strategy with three different numerical techniques along with multiple parameter perturbation strategies to control for possible numerical and sampling artifacts. Approximately 65% of the mechanisms in the G1/S restriction point were responsible for 95% of the sensitivity, conversely, the G2-DNA damage checkpoint showed a much stronger dependence on a few mechanisms; ∼32% or 13 of 40 mechanisms accounted for 95% of the sensitivity. Our analysis predicted that CDC25 and cyclin E mechanisms were strongly implicated in G1/S malfunctions, while fragility in the G2/M checkpoint was predicted to be associated with the regulation of the cyclin B-CDK1 complex. Analysis of a third model containing both G1/S and G2/M checkpoint logic, predicted in addition to mechanisms already mentioned, that translation and programmed proteolysis were also key fragile subsystems. Comparison of the predicted fragile mechanisms with literature and current preclinical and clinical trials suggested a strong correlation between efficacy and fragility. Thus, when taken together, these results support the working hypothesis that cell-cycle control architectures are HOT networks and establish the mathematical estimation and subsequent therapeutic exploitation of fragile mechanisms as a novel strategy for anti-cancer lead generation

Evaluation of individual and ensemble probabilistic forecasts of COVID-19 mortality in the United States

Short-term probabilistic forecasts of the trajectory of the COVID-19 pandemic in the United States have served as a visible and important communication channel between the scientific modeling community and both the general public and decision-makers. Forecasting models provide specific, quantitative, and evaluable predictions that inform short-term decisions such as healthcare staffing needs, school closures, and allocation of medical supplies. Starting in April 2020, the US COVID-19 Forecast Hub (https://covid19forecasthub.org/) collected, disseminated, and synthesized tens of millions of specific predictions from more than 90 different academic, industry, and independent research groups. A multimodel ensemble forecast that combined predictions from dozens of groups every week provided the most consistently accurate probabilistic forecasts of incident deaths due to COVID-19 at the state and national level from April 2020 through October 2021. The performance of 27 individual models that submitted complete forecasts of COVID-19 deaths consistently throughout this year showed high variability in forecast skill across time, geospatial units, and forecast horizons. Two-thirds of the models evaluated showed better accuracy than a naïve baseline model. Forecast accuracy degraded as models made predictions further into the future, with probabilistic error at a 20-wk horizon three to five times larger than when predicting at a 1-wk horizon. This project underscores the role that collaboration and active coordination between governmental public-health agencies, academic modeling teams, and industry partners can play in developing modern modeling capabilities to support local, state, and federal response to outbreaks