Chemical and sensory analysis of commercial Navel oranges in California.

Seven lots of commercially available Navel oranges grown in California were evaluated with flavoromic, metabolomic, sensory descriptive analysis, and consumer testing techniques to identify sensory and chemical drivers of liking. Eight identified chemical clusters related to numerous sensory attributes and consumer preferences. Differences in adult and child preferences led to the discovery of six consumer clusters (four adult and two child). Sweetness, overall flavor, sourness, fruity flavor, and juiciness were identified as the main sensory drivers of liking for the consumers. Fructose, glucose, and proline were among the compounds that best explained perceived sweetness while sourness was correlated with citrate and ascorbate. Perceived fruity flavor increased with higher concentrations of ethanol. We conclude that consumers differ in their preferences for Navel oranges and desire fruit that is higher in both sweetness and sourness

A Method for Calibration of the Local Magnitude Scale Based on Relative Spectral Amplitudes, and Application to the San Juan Bautista, California, Area

We develop and use a spectral empirical Green’s function approach to estimate the relative source amplitudes of earthquakes near San Juan Bautista, California. We isolate the source amplitudes from path effects by comparing the recorded spectra of pairs of events with similar location and focal mechanism, without computing the path effect. With this method, we estimate the relative moments of 1600 M 1.5–4 local earthquakes, and we use these moments to recalibrate the duration magnitude scale in this region. The estimated moments of these small earthquakes increase with catalog magnitude M_D roughly proportionally to 10^(1.1M_D), slightly more slowly than a moment‐magnitude scaling of 10^(1.5M_w). This more accurate magnitude scaling can be used in analyses of the local earthquakes, such as comparisons between the seismic moments and geodetic observations

Toward development of continuous bioprocesses: Comparison of fed-batch and perfusion upstream production processes in early development

Continuous Processing is an exciting development in the field of bioprocessing. The potential for quick response to market demands, decrease in infrastructure, increased flexibility and consistent product quality has resulted in a growing interest in Continuous Processing for production of all types of protein drugs (high or low volume, stable or unstable). Sanofi is developing a novel Integrated Continuous Manufacturing platform for biologics that utilizes an upstream perfusion process. While cell culture perfusion processes offer substantial benefits for commercial biologics production, implementation may present challenges in early development, where speed to first in man studies is critical. Here we present a comparison of candidate Phase I fed-batch and perfusion processes resulting from our upstream development work for a monoclonal antibody. The report focuses on process productivity, product quality attributes, and development timelines. Assessment of the advantages and challenges for both processes informs strategy for Continuous Process platform developmen

Characterization of the microbiome associated with in situ earthen materials

Background The current increase in public awareness of environmental risks is giving rise to a growth of interest in the microbiological safety of buildings. In particular, microbial proliferation on construction materials can be responsible for the degradation of indoor air quality that can increase health-risk to occupants. Raw earth materials are still widely used throughout the world and, in some cases, are linked to heritage habitats, as in the southwest of France. Moreover, these building materials are currently the subject of renewed interest for ecological and economic reasons. However, the microbial status of earthen materials raises major concerns: could the microbiome associated with such natural materials cause disease in building occupants? Very few analyses have been performed on the microbial communities present on these supports. Characterizing the raw earth material microbiome is also important for a better evaluation and understanding of the susceptibility of such materials to microbial development. This study presents the distribution of in situ bacterial and fungal communities on different raw earth materials used in construction. Various buildings were sampled in France and the microbial communities present were characterized by amplicon high-throughput sequencing (bacterial 16S rRNA gene and fungal ITS1 region). Bacterial culture isolates were identified at the species level by MALDI-TOF mass spectrometry. Results The major fungal and bacterial genera identified were mainly associated with conventional outdoor and indoor environmental communities, and no specific harmful bacterial species were detected on earthen materials. However, contrary to expectations, few human-associated genera were detected in dwellings. We found lower microbial alpha-diversity in earthen material than is usually found in soil, suggesting a loss of diversity during the use of these materials in buildings. Interestingly enough, the main features influencing microbial communities were building history and room use, rather than material composition. Conclusions These results constitute a first in-depth analysis of microbial communities present on earthen materials in situ and may be considered as a first referential to investigate microbial communities on such materials according to environmental conditions and their potential health impact. The bacterial and fungal flora detected were similar to those found in conventional habitats and are thought to be mainly impacted by specific events in the building’s life, such as water damage

Bi-Phasic Vesicles: instability induced by adsorption of proteins

The recent discovery of a lateral organization in cell membranes due to small structures called 'rafts' has motivated a lot of biological and physico-chemical studies. A new experiment on a model system has shown a spectacular budding process with the expulsion of one or two rafts when one introduces proteins on the membrane. In this paper, we give a physical interpretation of the budding of the raft phase. An approach based on the energy of the system including the presence of proteins is used to derive a shape equation and to study possible instabilities. This model shows two different situations which are strongly dependent on the nature of the proteins: a regime of easy budding when the proteins are strongly coupled to the membrane and a regime of difficult budding.Comment: 19 avr. 200

Pulsatile release of gonadotropin-releasing hormone from hypothalamic explants is restrained by blockade of N-Methyl-D,L-Aspartate receptors

We have shown previously that N-methyl-D,Laspartate (NMDA) and kainate, two neuroexcitatory amino acids acting through distinct receptors, may induce the release of GnRH from hypothalamic explants. However, that effect could have no physiological significance, since very high concentrations (50 mM) of NMDA and kainate were required. Here, using agents blocking the activation of receptors to neuroexcitatory amino acids, we evaluated their possible physiological involvement in the pulsatile release of GnRH from the hypothalamus of 50-day-old male rats in vitro. In control conditions (10 nM glycine and 1 mM Mg2+), the release of GnRH in 7.5-min fractions collected for 2–4 h showed an obvious pulsatile pattern. The mean (±1 SD) interval between pulses, identified by PULSAR program, was 34.3 ± 11.4 min. The stimulation of GnRH release by NMDA (50 mM) added to the medium for 7.5 min could be blocked reversibly in the presence of MK-801 (100 μM) using medium without glycine or enriched with Mg2+ (2 mM). The endogenous pulses of GnRH secretion were abolished in the presence of MK-801 or using increased Mg2+ concentrations as well as in the absence of glycine. In contrast, pulsatile release of GnRH was not affected in the presence of 6,7-dinitroquinoxaline-2,3-dione (0.1 mM), a selective inhibitor of kainate and quisqualate receptors which suppressed the increase in GnRH release induced by kainate (50 mM) without affecting the response to NMDA. These data indicate that the physiological mechanism of pulsatile GnRH secretion in the hypothalamus may involve endogenous neuroexcitatory factors acting through NMDA-sensitive receptors

A Method for Calibration of the Local Magnitude Scale Based on Relative Spectral Amplitudes, and Application to the San Juan Bautista, California, Area

We develop and use a spectral empirical Green’s function approach to estimate the relative source amplitudes of earthquakes near San Juan Bautista, California. We isolate the source amplitudes from path effects by comparing the recorded spectra of pairs of events with similar location and focal mechanism, without computing the path effect. With this method, we estimate the relative moments of 1600 M 1.5–4 local earthquakes, and we use these moments to recalibrate the duration magnitude scale in this region. The estimated moments of these small earthquakes increase with catalog magnitude M_D roughly proportionally to 10^(1.1M_D), slightly more slowly than a moment‐magnitude scaling of 10^(1.5M_w). This more accurate magnitude scaling can be used in analyses of the local earthquakes, such as comparisons between the seismic moments and geodetic observations

Experimental Effects of Pre-Drive Arousal on Teenage Simulated Driving Performance in the Presence of a Teenage Passenger

Teenage passengers increase teenage driving risk, but this may be conditional on events and emotions immediately preceding driving. An experimental simulation study evaluated the effect of pre-drive arousal on risky driving in the presence of a confederate teenage passenger. In a two-by-two between-subjects design, participants were randomized to high or low pre-drive arousal and passenger present or not present conditions. Prior to the drive participants played the Nintendo Wii video game, Rock BandTM. In the high-arousal condition participants stood while playing high-energy Beatles songs; in the low arousal condition participants sat while playing low-energy Beatles songs. The manipulation produced differences in arousal by group. Group differences in risky driving were in the expected direction, but were not statistically significant at p = .05 on any of the three outcome measures, which included Failed to Stop (failing to stop at signalized intersections in the dilemma zone), Percent Time in Red (in intersections), and Pass Slow Vehicle (electing to pass a slow vehicle)

Hackathons as a means of accelerating scientific discoveries and knowledge transfer

International audienceScientific research plays a key role in the advancement of human knowledge and pursuit of solutions to important societal challenges. Typically, research occurs within specific institutions where data are generated and subsequently analyzed. Although collaborative science bringing together multiple institutions is now common, in such collaborations the analytical processing of the data is often performed by individual researchers within the team, with only limited internal oversight and critical analysis of the workflow prior to publication. Here, we show how hackathons can be a means of enhancing collab-orative science by enabling peer review before results of analyses are published by cross-validating the design of studies or underlying data sets and by driving reproducibility of scientific analyses. Traditionally, in data analysis processes, data generators and bioinformaticians are divided and do not collaborate on analyzing the data. Hackathons are a good strategy to build bridges over the traditional divide and are potentially a great agile extension to the more structured collaborations between multiple investigators and institutions