Spectrophotometric estimation of lamotrigine in tablets

Simple and accurate spectrophotometric methods have been developed for the determination of lamotrigine in tablet formulation. The method A was based on the diazotization of lamotrigine followed by coupling with 4-(dimethylamino)benzaldehyde in acid medium to give greenish yellow product with a λmax of 410 nm. The method B also was based on the diazotization of drug and conversion of phenol into nitrosophenol with excess nitrous acid in acid medium to give brown color with a λmax of 448 nm. Thus, methods A and B were used to determine lamotrigine in the range of 1-10 μg/ml and 2.5-12 μg/ml, respectively in the final measured solution. There was no interference from the ingredients commonly found in lamotrigine tablets with these methods. The results compared favorably with those of reported methods and related analytical parameters were calculate

SCREENING OF PHYTOCHEMICAL CONTENT AND IN VITRO BIOLOGICAL INVESTIGATION OF CANTHIUM DICOCCUM (GAERTN.) AND AMISCHOPHACELUS AXILLARIS (L.)

Objective: The objective of the study was to study the pet ether, ethyl acetate, and ethanol leaf extracts of Canthium dicoccum and Amischophacelus axillaris for anthelmintic activity and antihypertensive activity. Methods: The antihypertensive activity was carried out by employing a colorimetric assay based on the hydrolysis of Histidyl-Hippuryl-Leucine and anthelmintic activity carried out against Indian earthworm Pheritimaposthuma. Results: The pet ether leaf extract both the plants exhibited the maximum antihypertensive activity with a percent inhibition of 64.82 for C. dicoccum (Gaertn.) and 84.12 for A. axillaris (L.) as compared with Captopril showing percent inhibition 85.37 and for anthelmintic activity, it is found that ethanol extract of C. dicoccum and ethyl acetate extract of A. axillaris exhibited significant activity against the standard drug albendazole. Conclusion: This study investigated the potential of C. dicoccum and A. axillaris as a new source against the antihypertensive activity. The outcome of anthelmintic activity revealed that the ethyl acetate and ethanol extracts exhibited a considerable amount of anthelmintic activity, which is mainly due to the active phytoconstituents present in the extracts

Synthesis and crystal structure of ebastinium hydrogen fumarate

The structure of ebastinium hydrogen fumarate {systematic name: 1-[4-(4-tert-butyl­phen­yl)-4-oxobut­yl]-4-(di­phenyl­meth­oxy)piperidin-1-ium (E)-3-carb­oxy-1-hy­droxy­prop-2-en-1-olate}, C32H40NO2+·C4H3O4−, a 1:1 salt formed in the reaction between ebastine and fumaric acid is presented. All examined crystals were found to be twinned by pseudo-merohedry. The structure is extensively disordered, with over half (20 out of 35) its non-hydrogen atoms modelled as lying over two sets of sites. In the crystal, cation–anion pairs are linked by a strong N—H⋯O hydrogen bond [N⋯O = 2.697 (11) Å]. These units inter­act via weaker C—H⋯O and C—H⋯π contacts to form layers lying parallel to the bc plane. The hydrogen fumarate anions are linked by a very short O—H⋯O hydrogen bond [O⋯O = 2.5402 (17) Å], augmented by weak C—H⋯O contacts into pairs of R22(6) ring motifs to form chains that extend parallel to the b-axis direction. Comparisons to similar crystal structures are presented

Synthesis and crystal structure of 2-chloro-1-(3-hy­dr­oxy­phen­yl)ethanone

The structure of 2-chloro-1-(3-hy­droxy­phen­yl)ethanone, C8H7ClO2, an α-halo­ketone is described. The mol­ecule is planar (r.m.s. deviation = 0.0164 Å) and in the crystal, inversion-symmetric dimers are formed as a result of pairs of strong O—H⋯O and weak C—H⋯O hydrogen bonds. A brief comparison is made with structurally related compounds deposited in the CSD. In addition, the synthesis and some spectroscopic details are presented

Landscape phage ligands for PC3 prostate carcinoma cells

Tumor-specific cytotoxicity of drugs can be enhanced by targeting them to tumor receptors using tumor-specific ligands. Phage display technology with its high throughput capacity for the analysis of targeting ligands possessing specific binding properties represents a very attractive tool in the quest for molecular ligands. Also, current phage nanobiotechnology concepts allow the use of intact phage particles and isolated phage coat proteins per se as components of nanomedicines. Herein, we describe the use of two landscape phage libraries to obtain phage ligands against PC3 prostate carcinoma cells. Following a very stringent selection scheme, we were able to identify three phage ligands, bearing the fusion peptides, DTDSHVNL, DTPYDLTG and DVVYALSDD that demonstrated specificity and selectivity to PC3 cells based on target-association assays, microscopy and flow cytometry. The phage ligands and their fusion coat proteins can be used as navigating modules in both therapeutic and diagnostic approaches to prostate carcinoma

Iso­propyl 4-amino­benzoate

The title compound, C10H13NO2, crystallizes with two mol­ecules (A and B) in the asymmetric unit. For A, the dihedral angle between the plane of the phenyl ring and the i-propyl substituent is 65.4 (3)° while for B this angle is 67.8 (3)°. In the crystal, the mol­ecules are linked by N—H⋯O and N—H⋯N hydrogen bonds to generate double chains propagating in the [100] direction

Synthesis and crystal structure of topiramate azido­sulfate at 90 K and 298 K

The low (90 K) and room (298 K) temperature crystal structures of topiramate azido­sulfate [systematic name 2,3:4,5-bis-O-(1-methyl­ethyl­idene)-β-D-fructo­pyran­ose azido­sulfate], C12H19N3O8S, an inter­mediate in the synthesis of the anti-convulsant drug topiramate, are described. Topiramate azido­sulfate (I) finds use as a reference impurity standard for topiramate. A modified synthesis and some spectroscopic details are also presented