The use of immunoregulatory properties of mesenchymal stem cells/ and their therapeutic potential

Mesenchymal stem cells (MSCs) have the potential to differentiate into various cell types, possess potent immunomodulatory properties and can influence various functions of immune cells. Since the immunomodulatory properties of MSCs can be modified by cytokines, we compered the effect of unstimulated MSCs and MSCs pretreated with interleukin (IL)-1, interferon (IFN)- , transforming growth factor (TGF)- and IL-10 on the development of regulatory T cells (Treg) and T helper 17 (Th17) cells in vitro and on the inflammatory environment in the eye. MSCs can produce significant levels of TGF- and IL-6. These cytokines represent the key factors that reciprocally regulate the development of naive T cells into Treg and Th17 cells. Unstimulated MSCs produce TGF- , but not IL-6, and the production of TGF- can be further enhanced by IL-10 or TGF- . In the presence of IL-1, MSCs secrete significant levels of IL-6, in addition to spontaneous production of TGF- . MSC producing TGF- induced preferentially expression of Foxp3 and activation of Treg lymphocytes, whereas MSCs supernatants containing TGF- together with IL-6 supported ROR t expression and development of Th17 cells. We demonstrated that MSCs and their products effectively control the development of Tregs and Th17 cells in a population of...Mezenchymální kmenové bu ky (mesenchymal stem cells - MSC) mají schopnost diferencovat v r zné bun né typy a zárove disponují rozsáhlými imunomodula ními vlastnostmi, jejichž prost ednictvím mohou ovliv ovat adu funkcí r zných bun k imunitního systému. Protože imunoregula ní vlastnosti MSC mohou být ovlivn ny p sobením cytokin , porovnávali jsme ú inek neovlivn ných MSC a MSC stimulovaných interleukinem 1 (interleukin - IL), interferonem- (interferon - IFN), transformujícím r stovým faktorem- (transforming growth factor - TGF) a IL-10 na vývoj regula ních T (regulatory T cells - Treg) a pomocných T17 (helper T cells - Th) lymfocyt in vitro a na rozvoj asného zán tu v oku in vivo. MSC mohou produkovat významná množství TGF- a IL-6. Tyto dva cytokiny p edstavují klí ové faktory, které recipro n regulují vývoj naivních T lymfocyt v Treg nebo Th17 bu ky. Nestimulované MSC produkují TGF- , ale neprodukují IL-6. Produkce TGF- m že být dále zesílena p sobením IL-10 a TGF- na MSC. V p ítomnosti prozán tlivých cytokin naopak MSC produkují významná množství IL-6 a zárove konstitutivn produkují TGF- . MSC produkující TGF- indukovaly p ednostn expresi Foxp3 a aktivaci Treg lymfocyt , zatímco supernatanty z MSC obsahující TGF- i IL-6 podporovaly expresi ROR t a vývoj Th17 lymfocyt . Ukázali jsme, že MSC a jimi...Department of Cell BiologyKatedra buněčné biologieFaculty of SciencePřírodovědecká fakult

The use of immunoregulatory properties of mesenchymal stem cells/ and their therapeutic potential

Mezenchymální kmenové bu ky (mesenchymal stem cells - MSC) mají schopnost diferencovat v r zné bun né typy a zárove disponují rozsáhlými imunomodula ními vlastnostmi, jejichž prost ednictvím mohou ovliv ovat adu funkcí r zných bun k imunitního systému. Protože imunoregula ní vlastnosti MSC mohou být ovlivn ny p sobením cytokin , porovnávali jsme ú inek neovlivn ných MSC a MSC stimulovaných interleukinem 1 (interleukin - IL), interferonem- (interferon - IFN), transformujícím r stovým faktorem- (transforming growth factor - TGF) a IL-10 na vývoj regula ních T (regulatory T cells - Treg) a pomocných T17 (helper T cells - Th) lymfocyt in vitro a na rozvoj asného zán tu v oku in vivo. MSC mohou produkovat významná množství TGF- a IL-6. Tyto dva cytokiny p edstavují klí ové faktory, které recipro n regulují vývoj naivních T lymfocyt v Treg nebo Th17 bu ky. Nestimulované MSC produkují TGF- , ale neprodukují IL-6. Produkce TGF- m že být dále zesílena p sobením IL-10 a TGF- na MSC. V p ítomnosti prozán tlivých cytokin naopak MSC produkují významná množství IL-6 a zárove konstitutivn produkují TGF- . MSC produkující TGF- indukovaly p ednostn expresi Foxp3 a aktivaci Treg lymfocyt , zatímco supernatanty z MSC obsahující TGF- i IL-6 podporovaly expresi ROR t a vývoj Th17 lymfocyt . Ukázali jsme, že MSC a jimi...Mesenchymal stem cells (MSCs) have the potential to differentiate into various cell types, possess potent immunomodulatory properties and can influence various functions of immune cells. Since the immunomodulatory properties of MSCs can be modified by cytokines, we compered the effect of unstimulated MSCs and MSCs pretreated with interleukin (IL)-1, interferon (IFN)- , transforming growth factor (TGF)- and IL-10 on the development of regulatory T cells (Treg) and T helper 17 (Th17) cells in vitro and on the inflammatory environment in the eye. MSCs can produce significant levels of TGF- and IL-6. These cytokines represent the key factors that reciprocally regulate the development of naive T cells into Treg and Th17 cells. Unstimulated MSCs produce TGF- , but not IL-6, and the production of TGF- can be further enhanced by IL-10 or TGF- . In the presence of IL-1, MSCs secrete significant levels of IL-6, in addition to spontaneous production of TGF- . MSC producing TGF- induced preferentially expression of Foxp3 and activation of Treg lymphocytes, whereas MSCs supernatants containing TGF- together with IL-6 supported ROR t expression and development of Th17 cells. We demonstrated that MSCs and their products effectively control the development of Tregs and Th17 cells in a population of...Katedra buněčné biologieDepartment of Cell BiologyFaculty of SciencePřírodovědecká fakult

Inhalation of ZnO nanoparticles: Splice junction expression and alternative splicing in mice

Despite the wide application of nanomaterials, toxicity studies of nanoparticles (NP) are often limited to in vitro cell models, and the biological impact of NP exposure in mammals has not been thoroughly investigated. Zinc oxide (ZnO) NPs are commonly used in various consumer products. To evaluate the effects of the inhalation of ZnO NP in mice, we studied splice junction expression in the lungs as a proxy to gene expression changes analysis. Female ICR mice were treated with 6.46 x 10(4) and 1.93 x 10(6) NP/cm(3) for 3 days and 3 months, respectively. An analysis of differential expression and alternative splicing events in 298 targets (splice junctions) of 68 genes involved in the processes relevant to the biological effects of ZnO NP was conducted using next-generation sequencing. Three days of exposure resulted in the upregulation of IL-6 and downregulation of BID, GSR, NF-kB2, PTGS2, SLC11A2, and TXNRD1 splice junction expression; 3 months of exposure increased the expression of splice junctions in ALDH3A1, APAF1, BID, CASP3, DHCR7, GCLC, GCLM, GSR, GSS, EHHADH, FAS, HMOX-1, IFN, NF-kB1, NQO-1, PTGS1, PTGS2, RAD51, RIPK2, SRXN1, TRAF6, and TXNRD1. Alternative splicing of TRAF6 and TXNRD1 was induced after 3 days of exposure to 1.93 x 10(6) NP/cm(3). In summary, we observed changes of splice junction expression in genes involved in oxidative stress, apoptosis, immune response, inflammation, and DNA repair, as well as the induction of alternative splicing in genes associated with oxidative stress and inflammation. Our data indicate the potential negative biological effects of ZnO NP inhalation.Web of Science168120019

The use of immunoregulatory properties of mesenchymal stem cells/ and their therapeutic potential

Mesenchymal stem cells (MSCs) have the potential to differentiate into various cell types, possess potent immunomodulatory properties and can influence various functions of immune cells. Since the immunomodulatory properties of MSCs can be modified by cytokines, we compered the effect of unstimulated MSCs and MSCs pretreated with interleukin (IL)-1, interferon (IFN)- , transforming growth factor (TGF)- and IL-10 on the development of regulatory T cells (Treg) and T helper 17 (Th17) cells in vitro and on the inflammatory environment in the eye. MSCs can produce significant levels of TGF- and IL-6. These cytokines represent the key factors that reciprocally regulate the development of naive T cells into Treg and Th17 cells. Unstimulated MSCs produce TGF- , but not IL-6, and the production of TGF- can be further enhanced by IL-10 or TGF- . In the presence of IL-1, MSCs secrete significant levels of IL-6, in addition to spontaneous production of TGF- . MSC producing TGF- induced preferentially expression of Foxp3 and activation of Treg lymphocytes, whereas MSCs supernatants containing TGF- together with IL-6 supported ROR t expression and development of Th17 cells. We demonstrated that MSCs and their products effectively control the development of Tregs and Th17 cells in a population of..

The use of immunoregulatory properties of mesenchymal stem cells/ and their therapeutic potential

Mesenchymal stem cells (MSCs) have the potential to differentiate into various cell types, possess potent immunomodulatory properties and can influence various functions of immune cells. Since the immunomodulatory properties of MSCs can be modified by cytokines, we compered the effect of unstimulated MSCs and MSCs pretreated with interleukin (IL)-1, interferon (IFN)- , transforming growth factor (TGF)- and IL-10 on the development of regulatory T cells (Treg) and T helper 17 (Th17) cells in vitro and on the inflammatory environment in the eye. MSCs can produce significant levels of TGF- and IL-6. These cytokines represent the key factors that reciprocally regulate the development of naive T cells into Treg and Th17 cells. Unstimulated MSCs produce TGF- , but not IL-6, and the production of TGF- can be further enhanced by IL-10 or TGF- . In the presence of IL-1, MSCs secrete significant levels of IL-6, in addition to spontaneous production of TGF- . MSC producing TGF- induced preferentially expression of Foxp3 and activation of Treg lymphocytes, whereas MSCs supernatants containing TGF- together with IL-6 supported ROR t expression and development of Th17 cells. We demonstrated that MSCs and their products effectively control the development of Tregs and Th17 cells in a population of..

Strategie a metodická podpora udržení a rozvoje zeleně v urbanizovaném prostoru:Návrh systému pro zpracování a údržbu dat k evidenci zeleně (dendrologických prvků) za účelem zefektivnění udržení zeleně v moderním pojetí informačních technologií s využitím geografických informačních systémů - GIS

Cílem subprojektu je vytvořit metodický postup pro pořizování, údržbu a zpřístupnění informací o zeleni a její evidenci v urbanizovaném prostoru. Byly zhodnoceny a navrženy možnosti využití informačních technologií jak pro výkon veřejné správy, tak i pro zpřístupnění dalších informací o biotické složce (zeleni) veřejnosti