The Role of Lead Toxicity on Eruption Rate of Hypofunctional Incisors in Albino Wistar Rats

OBJECTIVES This objective of this study was to evaluate the role of a heavy metal- lead acetate in the eruption rate of hypo functional incisors in albino Wistar rats. METHODOLOGY An experimental study was done in animal house of Post Graduate Medical Institute, Lahore since March 2019 to March 2020. 34 adult albino Wistar rats were randomly divided into two groups (n=17 for each group) i.e., control and lead acetate group. Right mandibular incisors were selected for this study. Selected incisors were marked 1mm above the level of gingival papillae. The incisors were cut above this mark to make it hypo-functional. The readings were measured by digital Vernier caliper. This was considered as day 0. Incisors length was measured at day 0, 3, 6, 12 and 15 and eruption was calculated. The data was analyzed using SPSS version 22. RESULTS Eruption rate was similar throughout the study except last follow up. At the end of this study eruption of incisors in albino Wistar rats in control was 03.30±0.72mm, in lead 02.43±1.19mm. At day 15, the difference between control and lead group was statistically significant (p-value 0.033). CONCLUSION These results reveal that besides other causes of delayed tooth eruption excessive lead intoxication are also acausative factor of delayed tooth eruption

Antioxidant and hypoglycemic potential of phytogenic cerium oxide nanoparticles

The article processing charge was funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) – 491192747 and the Open Access Publication Fund of Humboldt-Universität zu Berlin.Plants provide humans with more than just food and shelter; they are also a major source of medications. The purpose of this research was to investigate the antioxidant and hypoglycemic potential of green synthesized CeONPs using Mentha royleana leaves extract. The morphological and physicochemical features of CeONPs were evaluated by UV–Visible spectrophotometry, Scanning Electron Microscopy, Energy Dispersive X-rays and Fourier-transform infrared spectrometry, Dynamic light scattering, Atomic Force Microscopy, Zeta Potential. The average size range of synthesized CeONPs diameter between 46 and 56 nm, crystalline in shape, with Polydispersity index value of 0.2 and subatomic particles mean diameter was 4.5–9.1 nm. The antioxidant capability of CeONPs was assessed using DPPH, ABTS+, hydrogen peroxide, hydroxyl radical scavenging, and reducing power tests. The hypoglycemic potential of CeONPs was investigated using alpha-amylase, alpha-glucosidase, glucose absorption by yeast cells, and antisucrase. The effective concentrations were 500 and 1000 µg/ml found good in suppressing radical species. To explore the hypoglycemic potential of CeONPs, alpha-amylase, alpha-glucosidase, glucose absorption by yeast cell, and antisucrase assays were performed. Glucose absorb by yeast cells assay was tested for three distinct glucose concentrations: 5 mmol/L, 10 mmol/L, and 25 mmol/L. Green synthesize CeONPs showed a dose-dependent response, higher concentrations of CeONPs imposed a stronger inhibitory impact on the catalytic site of enzymes. This study suggest that CeONPs could possibly binds to the charge carrying species and act as competitive inhibitor which slow down the enzyme substrate reaction and prevents enzymatic degradation. The study’s findings were outstanding, which bodes well for future medicinal applications of CeONPs.Peer Reviewe

The Impact of Service Quality and Service Value on Customer Satisfaction through Customer bonding: Evidence from telecommunication Sector

In today’s competitive environment customer occupies the central place in business and retaining a satisfied customer can pay more as compared to attracting new one. The main objective of this study is to examine the impact of Service Quality and Service Value on Customer Satisfaction through mediating role of Customer bonding. Self-administered questionnaires were used for collection of data. Questionnaires were distributed among customers of telecommunication companies. Total 420 questionnaires were distributed among students, employees and businessmen. 397 questionnaires were received back out of which 3 questionnaires were rejected due to incomplete information. So, the response rate was 94%. For the purpose of analysis, SPSS and AMOS software have been used and mediation has been tested through online Sobel calculator. The result shows that Service Value and Service Quality have positive significant impact on customer satisfaction and mediation analysis shows that Customer bonding partially mediates the association of Service Quality and Service Value with customer satisfaction. This model may also be applied in banking and other service sectors for increasing the customer satisfaction

Procjena različitih razina Bacillus sp. (HCYL03) fitaze u brojlera hranjenih obrocima na bazi kukuruza i soje s niskim udjelom nefitatnog fosfora

The objective of the present study was to evaluate the impact of various levels of phytase derived from Bacillus sp. (HCYL03) in corn-soy diets fed to broilers. Experimental treatments included a positive control (PC) with a calculated non-phytate phosphorus (nPP) level of 4.0g/kg for the 35 days of trial. The negative control (NC) diet included a reduction in nPP to 3.0g/kg during the experiment, and commercially available phytase (@500FTU/kg), as well as new bacterial phytase added to the NC diet in increasing amounts of 500, 800, and 1100FTU/kg. Treatment effects on growth performance, the apparent digestibility of P, tibia mineralization, and Ca and P status in blood plasma were evaluated on day 35. The NC diet decreased feed intake (P<0.05), body weight gain (BWG) (P<0.05), and improved feed conversion ratio (FCR) (P<0.05) compared to the PC. Phytase addition improved all growth parameters. Birds fed the NC diet displayed lower (P<0.05) digestibility of P, reduced (P<0.05) tibial mineralization, and decreased (P<0.05) P and Ca concentrations in blood plasma compared to birds fed the PC diet. Improvements in digestibility of P, tibia mineralization, and mineral contents in blood plasma were observed with phytase addition. High level inclusion of phytase (1100FTU/kg) yielded the greatest improvement in bird performance, nutrient digestibility, and bone mineralization in the NC group and low levels of phytase treatments. It may be concluded that inorganic P incorporated in the normal-nPP diet of chickens could be effectively replaced by a Bacillus sp. (HCYL03) phytase diet without any adverse effect on the performance and nutrient use of broilers.Cilj istraživanja bio je procijeniti utjecaj različitih razina fitaze izdvojene iz Bacillus sp. (HCYL03) i dodane u obroke od kukuruza i soje kojima se hrane brojleri. Istraživanje je uključilo pozitivnu kontrolu (PC) s izračunatom razinom nefitatnog fosfora (nPP) od 4,0 g/kg tijekom 35 dana trajanja istraživanja. Prehrana brojlera u negativnoj kontroli (NC) uključila je smanjenje nPP-a na 3,0 g/kg tijekom trajanja pokusa, komercijalno dostupnu fitazu (@500FTU/kg), kao i novu bakterijsku fitazu dodanu NC prehrani, u količini koja se povećavala na 500, 800 i 1100 FTU/kg. Učinci na rast, probavljivost fosfora, mineralizaciju tibije i razinu kalcija i fosfora u krvnoj plazmi procijenjeni su 35. dan pokusa. U skupini NC smanjeni su unos hrane (P<0,05) i prirast tjelesne mase (BWG) (P<0,05), dok je stopa konverzije hrane povećana (FCR) (P<0,05) u usporedbi sa skupinom PC. Dodatak fitaze pozitivno je utjecao na sve pokazatelje rasta. Brojleri u skupini NC pokazali su manju probavljivost fosfora (P<0,05), smanjenu mineralizaciju tibije (P<0,05) te smanjenu količinu fosfora i kalcija (P<0,05) u krvnoj plazmi u usporedbi s brojlerima iz skupine PC. Utvrđeno je da dodatak fitaze poboljšava probavljivost fosfora, mineralizaciju tibije i sadržaj minerala u krvnoj plazmi. Dodatak veće količine fitaze (1100 FTU/kg) rezultirao je najvećim poboljšanjem u istraženim svojstvima brojlera, probavljivosti hrane i mineralizaciji kosti u skupini NC. Zaključeno je da bi se anorganski fosfor uključen u uobičajenu nPP prehranu pilića mogao učinkovito zamijeniti Bacillus sp. (HCYL03) fitazom, bez štetnih učinaka na prehranu i svojstva brojlera

Repair of Accidentally Perforated Labial Wall of a Central Incisor Having Failed Root Treatment -A Case Report

Abstract This is a successfully managed clinical case report of a young lady whose tooth # 11 was root treated in 2010 which reinfected after three years. The root treatment was repeated but was iatrogenically perforated because of the operating dentist&apos;s error. The tooth was therefore opened for surgical correction. During the procedure, it was found that labial wall of the tooth was missing in the mid-root area. The labial wall was built using glass ionomer cement keeping the endodontic file in the canal to avoid obliteration of canal space by glass ionomer. The rebuilt canal was treated with Calcium Hydroxide dressings at an interval of 7 days. In two weeks as the sinus healed, swelling subsided and the canal became effortlessly dryable using just two absorbent points, the canal was obturated with a custom made gutta percha cone, employing glass ionomer as a canal sealer

Quality assessment and antimicrobial activity of various honey types of Pakistan

Forty samples of different honey types (Acacia, Ziziphus, Brassica and Citrus) were collected from different areas of Pakistan and analyzed for moisture, pH, total acidity, ash, electrical conductivity, hydroxymethylfurfural (HMF), sucrose, total sugars, invert sugar, protein, proline contents as well as macro and micro elements. The variation in composition of honey samples was observed due to different types of flora. Higher pH (6.56 ± 0.05) was observed for Ziziphus honey, acidity (45.0 ± 2.35 mg/kg) for Citrus, moisture (36.8 ± 1.8%) for Brasica and HMF (32.7 ± 0.49 mg/kg) for Acacia. Whereas, higher concentrations of proline (2.1 ± 0.04 mg/kg) and invert sugar (0.38 ± 0.1%) for Citrus honey and protein (16.5 ± 1.5 g/100g) for Acacia honey were observed. Likewise, a significant level (P &lt; 0.05) of ash, electric conductivity, sucrose, total sugar as well as macro and micro elements was also found in these honey types. Different formulations of honey has significantly inhibited growth of pathogenic microorganisms, Staphylococcus aureus, Escherichia coli, Candida albicans and Aspergillus niger when compared to control group, which is an evidence that honey is a therapeutic agent being used since ancient time throughout the world.Key words: Honey types, flora, sugar, nutritional food, therapeutic agent

Doppler of Renal Segmental Artery Resistive Index in Diabetic Patients

Background: Diabetic nephropathy affects 40 percent of patients with type I diabetes mellitus. It carries a poor prognosis when fully developed, where the estimated death rate is approximately 40-100 times that of non-diabetics. Vascular resistivity evaluation at multiple points of renal parenchyma can indicate structural or functional changes within the kidneys and can provide useful diagnostic and prognostic information. Objective: The goal of the research was to use renal artery Doppler to evaluate the mean renal resistivity index in patients with Diabetes Mellitus. Methodology: The research was initiated with the approval of the University of Lahore Ethical Committee. In this study, all the patients were registered after signing the informed consent form. Toshiba Xario was used with a convex probe of frequency of 3-5 MHz. Renal Resistive Index (RRI) was evaluated and sonographic data was kept in the ultrasound unit. The Statistical package for Social Sciencies version 24 was used for analysing the data. Results: Among 97 patients, there were 66 females and 31 males of which 50 patients were with Diabetes and 47 were normal. In the result of our study it was seen that renal resistive index significantly increases in patients with Diabetes Mellitus. Conclusion: In  conclusion,  renal  resistive index (RRI) levels were high in patients with Diabetes Mellitus showing positive correlation of RRI values Keywords: Diabetic nephropathy, Renal resistive index, Chronic kidney disease DOI: 10.7176/JHMN/82-03 Publication date: November 30th 202

Response Surface Methodology for the production of endopolygalacturonase by a novel Bacillus licheniformis

Background: Polygalacturonase is one of the most important commercial pectinase. The production cost and the mesophilic nature of the present polygalacturonase is a big problem in its application in the juice industry. A lot of work is going on for the isolation of thermophilic bacterial strains which can utilize pectin as the only carbon source.Methods: Bacterial strains were isolated from rotten fruits and vegetables and cultured at 50 – 70oC. The strains were than screened for endopolygalacturonase activity and identified on the basis of 16S rRNA sequence. Different growth parameters for the production of endopolygalacturonase by Bacillus licheniformis IEB-8 were optimized using Response Surface Methodology under Center Composite Design using JMP-12 software. Endopolygalacturonase was purified in two steps; ammonium sulfate precipitation and then by size exclusion column chromatography.Results: Only four strains, IEB-8, IEB-11, IEB-12 and IEB-13 showed growth above 60oC. Among these four, only IEB-8 was found to be endopolygalacturonase positive, which was identified as Bacillus licheniformis by 16S rRNA gene sequence. Purification fold of 2.57 and 7.48 in the specific activity were achieved using ammonium sulfate precipitation and gel filtration chromatography respectively. Molecular weight of the purified endopolygalacturonase was found to be 42 kDa. The purified endopolygalacturonase showed an optimum pH of 7 and optimum temperature of 55oC.Conclusion: Bacillus licheniformis IEB-8 is a novel bacteria which can efficiently be utilized in the industry for the production of endopolygalacturonase very cheaply. Furthermore, the high optimum working temperature of endopolygalacturonase, increases its significance for its industrial applications.Keywords: Endopolygalacturonase; Bacillus licheniformis; Thermophilic; Response Surface Methodology; Ammonium sulfate precipitatio