DND1 Expression and Function in the Porcine Ovary, Oocyte and Embryo

DND1 (dead end homolog 1), belonging to the RNA binding protein family, can impact miRNA:mRNA functional pathway and in turn may contribute to maintaining normal oocyte growth and quality as well as embryo development following fertilization. To characterize DND1 in pig maturing oocytes and cumulus cells, and early embryos, we examined DND1 mRNA and protein expression using quantitative RT-PCR, Western blot and immunostaining. We found: (1) DND1 protein is expressed during pig follicle development; (2) DND1 is dynamically expressed at both mRNA and protein level in the maturing oocyte and early in vitro fertilized embryos; (3) DND1 mRNA is expressed in cumulus cells surrounding the maturing oocyte; and (4) DND1 protein is localized in the cytoplasm of pig maturing oocytes and early embryos. Our work provides useful data for functional study of DND1 proteins in female gametogenesis and developing embryos, which will benefit animal reproduction health and provides foundational knowledge for improving swine reproductive efficiency

Solid Freeform Fabrication of Composite-Material Objects

Composite solid freeform fabrication (C-SFF) or composite layer manufacturing (CLM) is an automated process in which an advanced composite material (a matrix reinforced with continuous fibers) is formed into a freestanding, possibly complex, three-dimensional object. In CLM, there is no need for molds, dies, or other expensive tooling, and there is usually no need for machining to ensure that the object is formed to the desired net size and shape. CLM is a variant of extrusion-type rapid prototyping, in which a model or prototype of a solid object is built up by controlled extrusion of a polymeric or other material through an orifice that is translated to form patterned layers. The second layer is deposited on top of the first layer, the third layer is deposited on top of the second layer, and so forth, until the stack of layers reaches the desired final thickness and shape. The elements of CLM include (1) preparing a matrix resin in a form in which it will solidify subsequently, (2) mixing the fibers and matrix material to form a continuous pre-impregnated tow (also called "towpreg"), and (3) dispensing the pre-impregnated tow from a nozzle onto a base while moving the nozzle to form the dispensed material into a patterned layer of controlled thickness. When the material deposited into a given layer has solidified, the material for the next layer is deposited and patterned similarly, and so forth, until the desired overall object has been built up as a stack of patterned layers. Preferably, the deposition apparatus is controlled by a computer-aided design (CAD) system. The basic CLM concept can be adapted to the fabrication of parts from a variety of matrix materials. It is conceivable that a CLM apparatus could be placed at a remote location on Earth or in outer space where (1) spare parts are expected to be needed but (2) it would be uneconomical or impractical to store a full inventory of spare parts. A wide variety of towpregs could be prepared and stored on spools until needed. Long-shelf-life towpreg materials suitable for such use could include thermoplastic-coated carbon fibers and metal-coated SiC fibers. When a spare part was needed, the part could be fabricated by CLM under control by a CAD data file; thus, the part could be built automatically, at the scene, within hours or minutes

Application of Particle Swarm Techniques in Sensor Network Configuration

A decentralized version of particle swarm optimization called the distributed particle swarm optimization (DPSO) approach is formulated and applied to the generation of sensor network configurations or topologies so that the deleterious effects of hidden nodes and asymmetric links on the performance of wireless sensor networks are minimized. Three different topology generation schemes, COMPOW, Cone-Based and the DPSO--based schemes are examined using ns-2. Simulations are executed by varying the node density and traffic rates. Results contrasting heterogeneous vs. homogeneous power reveal that an important metric for a sensor network topology may involve consideration of hidden nodes and asymmetric links, and demonstrate the effect of spatial reuse on the potency of topology generators

Design and analysis of a two-dimensional camera array

Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2005.Includes bibliographical references (p. 153-158).I present the design and analysis of a two-dimensional camera array for virtual studio applications. It is possible to substitute conventional cameras and motion control devices with a real-time, light field camera array. I discuss a variety of camera architectures and describe a prototype system based on the "finite-viewpoints" design that allows multiple viewers to navigate virtual cameras in a dynamically changing light field captured in real time. The light field camera consists of 64 commodity video cameras connected to off-the-shelf computers. I employ a distributed rendering algorithm that overcomes the data bandwidth problems inherent in capturing light fields by selectively transmitting only those portions of the video streams that contribute to the desired virtual view. I also quantify the capabilities of a virtual camera rendered from a camera array in terms of the range of motion, range of rotation, and effective resolution. I compare these results to other configurations. From this analysis I provide a method for camera array designers to select and configure cameras to meet desired specifications. I demonstrate the system and the conclusions of the analysis with a number of examples that exploit dynamic light fields.by Jason Chieh-Sheng Yang.Ph.D

National Football League Skilled and Unskilled Positions Vary in Opportunity and Yield in Return to Play After an Anterior Cruciate Ligament Injury.

BACKGROUND: Anterior cruciate ligament (ACL) injuries pose a significant risk to the careers of players in the National Football League (NFL). The relationships between draft round and position on return to play (RTP) among NFL players are not well understood, and the ability to return to preinjury performance levels remains unknown for most positions. PURPOSE: To test for differences in RTP rates and changes in performance after an ACL injury by position and draft round. We hypothesized that skilled positions would return at a lower rate compared to unskilled positions. We further hypothesized that early draft-round status would relate to a greater rate of RTP and that skilled positions and a lower draft round would correlate with decreased performance for players who return to sport. STUDY DESIGN: Case-control study; Level of evidence, 3. METHODS: Utilizing a previously established database of publicly available information regarding ACL tears among NFL players, athletes with ACL tears occurring between the 2010 and 2013 seasons were identified. Generalized linear models and Kaplan-Meier time-to-event models were used to test the study hypotheses. RESULTS: The overall RTP rate was 61.7%, with skilled players and unskilled players returning at rates of 64.1% and 60.4%, respectively (P = .74). Early draft-round players and unskilled late draft-round players had greater rates of RTP compared to skilled late draft-round players and both unskilled and skilled undrafted free agents (UDFAs). Skilled early draft-round players constituted the only cohort that played significantly fewer games after an injury. Unskilled UDFAs constituted the only cohort to show a significant increase in the number of games started and ratio of games started to games played, starting more games in which they played, after an injury. CONCLUSION: Early draft-round and unskilled players were more likely to return compared to their later draft-round and skilled peers. Skilled early draft-round players, who displayed relatively high rates of RTP, constituted the only cohort to show a decline in performance. Unskilled UDFAs, who exhibited relatively low rates of RTP, constituted the only cohort to show an increase in performance. The significant effect of draft round and position type on RTP may be caused by a combination of differences in talent levels and in opportunities given to returning to play

Heat Stress during Pig Oocyte In Vitro Maturation Impacts Embryonic Development and Gene Expression

Gene expression of heat stressed oocytes matured in vitro was evaluated for potential markers which could be used to characterize the effects of heat stress on developing oocytes in the pig. Three heat stress scenarios were administered during in vitro oocyte maturation. Heat stressed oocytes had reduced maturation rates, decreased developmental competency, and altered expression of heat stress and developmental competency markers

Prevention of mammary carcinogenesis by short-term estrogen and progestin treatments

INTRODUCTION: Women who have undergone a full-term pregnancy before the age of 20 have one-half the risk of developing breast cancer compared with women who have never gone through a full-term pregnancy. This protective effect is observed universally among women of all ethnic groups. Parity in rats and mice also protects them against chemically induced mammary carcinogenesis. METHODS: Seven-week-old virgin Lewis rats were given N-methyl-N-nitrosourea. Two weeks later the rats were treated with natural or synthetic estrogens and progestins for 7–21 days by subcutaneous implantation of silastic capsules. RESULTS: In our current experiment, we demonstrate that short-term sustained exposure to natural or synthetic estrogens along with progestins is effective in preventing mammary carcinogenesis in rats. Treatment with 30 mg estriol plus 30 mg progesterone for 3 weeks significantly reduced the incidence of mammary cancer. Short-term exposure to ethynyl estradiol plus megesterol acetate or norethindrone was effective in decreasing the incidence of mammary cancers. Tamoxifen plus progesterone treatment for 3 weeks was able to confer only a transient protection from mammary carcinogenesis, while 2-methoxy estradiol plus progesterone was effective in conferring protection against mammary cancers. CONCLUSIONS: The data obtained in the present study demonstrate that, in nulliparous rats, long-term protection against mammary carcinogenesis can be achieved by short-term treatments with natural or synthetic estrogen and progesterone combinations

Inferring yeast cell cycle regulators and interactions using transcription factor activities

BACKGROUND: Since transcription factors are often regulated at the post-transcriptional level, their activities, rather than expression levels may provide valuable information for investigating functions and their interactions. The recently developed Network Component Analysis (NCA) and its generalized form (gNCA) provide a robust framework for deducing the transcription factor activities (TFAs) from various types of DNA microarray data and transcription factor-gene connectivity. The goal of this work is to demonstrate the utility of TFAs in inferring transcription factor functions and interactions in Saccharomyces cerevisiae cell cycle regulation. RESULTS: Using gNCA, we determined 74 TFAs from both wild type and fkh1 fkh2 deletion mutant microarray data encompassing 1529 ORFs. We hypothesized that transcription factors participating in the cell cycle regulation exhibit cyclic activity profiles. This hypothesis was supported by the TFA profiles of known cell cycle factors and was used as a basis to uncover other potential cell cycle factors. By combining the results from both cluster analysis and periodicity analysis, we recovered nearly 90% of the known cell cycle regulators, and identified 5 putative cell cycle-related transcription factors (Dal81, Hap2, Hir2, Mss11, and Rlm1). In addition, by analyzing expression data from transcription factor knockout strains, we determined 3 verified (Ace2, Ndd1, and Swi5) and 4 putative interaction partners (Cha4, Hap2, Fhl1, and Rts2) of the forkhead transcription factors. Sensitivity of TFAs to connectivity errors was determined to provide confidence level of these predictions. CONCLUSION: By subjecting TFA profiles to analyses based upon physiological signatures we were able to identify cell cycle related transcription factors consistent with current literature, transcription factors with potential cell cycle dependent roles, and interactions between transcription factors

Composition constraints of the TRAPPIST-1 planets from their formation

We study the formation of the TRAPPIST-1 (T1) planets starting shortly after Moon-sized bodies form just exterior to the ice line. Our model includes mass growth from pebble accretion and mergers, fragmentation, type-I migration, and eccentricity and inclination dampening from gas drag. We follow the composition evolution of the planets fed by a dust condensation code that tracks how various dust species condense out of the disc as it cools. We use the final planet compositions to calculate the resulting radii of the planets using a new planet interior structure code and explore various interior structure models. Our model reproduces the broader architecture of the T1 system and constrains the initial water mass fraction of the early embryos and the final relative abundances of the major refractory elements. We find that the inner two planets likely experienced giant impacts and fragments from collisions between planetary embryos often seed the small planets that subsequently grow through pebble accretion. Using our composition constraints we find solutions for a two-layer model, a planet comprised of only a core and mantle, that match observed bulk densities for the two inner planets b and c. This, along with the high number of giant impacts the inner planets experienced, is consistent with recent observations that these planets are likely dessicated. However, two-layer models seem unlikely for most of the remaining outer planets which suggests that these planets have a primordial hydrosphere. Our composition constraints also indicate that no planets are consistent with a core-free interior structure.Comment: 15 pages, 8 figures, 4 tables, and an appendix. Accepted for publication in MNRA

Field-scale mapping of evaporative stress indicators of crop yield: An application over Mead, NE, USA

The Evaporative Stress Index (ESI) quantifies temporal anomalies in a normalized evapotranspiration (ET) metric describing the ratio of actual-to-reference ET (fRET) as derived from satellite remote sensing. At regional scales (3–10 km pixel resolution), the ESI has demonstrated the capacity to capture developing crop stress and impacts on regional yield variability in water-limited agricultural regions. However, its performance in some regions where the vegetation cycle is intensively managed appears to be degraded due to spatial and temporal limitations in the standard ESI products. In this study, we investigated potential improvements to ESI by generating maps of ET, fRET, and fRET anomalies at high spatiotemporal resolution (30-m pixels, daily time steps) using a multi-sensor data fusion method, enabling separation of landcover types with different phenologies and resilience to drought. The study was conducted for the period 2010–2014 covering a region around Mead, Nebraska that includes both rainfed and irrigated crops. Correlations between ESI and measurements of maize yield were investigated at both the field and county level to assess the potential of ESI as a yield forecasting tool. To examine the role of crop phenology in yield-ESI correlations, annual input fRET time series were aligned by both calendar day and by biophysically relevant dates (e.g. days since planting or emergence). At the resolution of the operational U.S. ESI product (4 km), adjusting fRET alignment to a regionally reported emergence date prior to anomaly computation improves r2 correlations with county-level yield estimates from 0.28 to 0.80. At 30-m resolution, where pure maize pixels can be isolated from other crops and landcover types, county-level yield correlations improved from 0.47 to 0.93 when aligning fRET by emergence date rather than calendar date. Peak correlations occurred 68 days after emergence, corresponding to the silking stage for maize when grain development is particularly sensitive to soil moisture deficiencies. The results of this study demonstrate the utility of remotely sensed ET in conveying spatially and temporally explicit water stress information to yield prediction and crop simulation models