Haematological response of cyclists after competition

The aim of the study was to analyze the hematologic response of cyclists after a nationwide competition level. Participated in 20 athletes (35 ± 7,90 years old, 1,75 ± 0,06m of height, 18,71 ± 4,12% of body fat, VO2max 57,90 ± 6,80 ml/kg/min, 345 ± 50,90W, 202 ± 6,77bpm of maximum heart rate), which underwent two blood samples, monitoring of body weight and water intake. After the competition was observed decrease in body weight (78,20 ± 9,10 to 76,70 ± 9,20 kg, p = 0,0001) with an average consumption of 620 ± 532,20 mL of water, and a significant increase (p = 0,0001) in the concentration of erythrocytes (5,28 ± 0,44 6 ± 0,41 million/mm3), hemoglobin (15,80 ± 1 to 17,70 ± 0,95 g/%), the platelets (211.950 ± 42.488,2 to 285.050 ± 47.097,2 un/mm3) and hematocrit (47 ± 2,97 to 54 ± 2,79%). Strong correlation (r > 0.7) between hematocrit with erythrocytes level and hemoglobin was observed. It was concluded that there is an increase in the number of hematological components of cyclists after competition with weak correlation with the water intake, body weight and the maximum volume of oxygen levelO objetivo do estudo foi analisar a reposta hematológica de ciclistas após uma competição de nível nacional. Participaram 20 atletas (35 ± 7,90 anos, 1,75 ± 0,06m de estatura, 18,71 ± 4,12% de gordura, VO2máx de 57,90 ± 6,80 ml/kg/min, 345 ± 50,90W, 202 ± 6,77 bpm de frequência cardíaca máxima), que passaram por duas coletas de sangue, monitoramento do peso corporal e da ingesta de água. Após a competição foi verificada queda no peso corporal (78,20 ± 9,10 para 76,70 ± 9,20 kg, p = 0,0001) com consumo médio de 620 ± 532,20 ml de água, e aumento significativo (p = 0,0001) na concentração de eritrócitos (5,28 ± 0,44 para 6 ± 0,41 milhões/mm3), de hemoglobina (15,80 ± 1 para 17,70 ± 0,95 g/%), de plaquetas (211.950 ± 42.488,20 para 285.050 ± 47.097,20 un/mm3) e hematócrito (47 ± 2,97 para 54 ± 2,79%). Foi verificada forte correlação (r > 0,7) entre o hematócrito com o nível de eritrócitos e de hemoglobina. Concluiu-se que existe aumento na quantidade dos componentes hematológicos em ciclistas após competição, com fraca correlação com a ingesta voluntária de água, peso corporal e nível do volume máximo de oxigêni

Genomics and epidemiology for gastric adenocarcinomas (GE4GAC): a Brazilian initiative to study gastric cancer

Abstract Gastric cancer (GC) is the fifth most common type of cancer worldwide with high incidences in Asia, Central, and South American countries. This patchy distribution means that GC studies are neglected by large research centers from developed countries. The need for further understanding of this complex disease, including the local importance of epidemiological factors and the rich ancestral admixture found in Brazil, stimulated the implementation of the GE4GAC project. GE4GAC aims to embrace epidemiological, clinical, molecular and microbiological data from Brazilian controls and patients with malignant and pre-malignant gastric disease. In this letter, we summarize the main goals of the project, including subject and sample accrual and current findings

Updated cardiovascular prevention guideline of the Brazilian Society of Cardiology: 2019

Sem informação113478788

Estudo de Variação Diagnóstica e Terapêutica para Acidente Vascular Cerebral Isquêmico no Serviço de Urgência.

O Acidente Vascular Cerebral (AVC) é reconhecido por suas manifestações clínicas, visto que são peculiares e apresentam-se de forma repentina como uma disfunção neurológica causada pelo bloqueio na passagem de sangue e nutrientes para o cérebro. Os AVCs acometem anualmente 17 milhões de pessoas no mundo, destes 6,5 milhões morrem, além dos que vivem com incapacidade permanente, sendo a maioria de origem isquêmica. É considerado um grande problema de saúde pública e seu diagnóstico e tratamento são determinantes para aumentar as chances de um bom prognóstico. O objetivo deste estudo foi determinar se existe variação na conduta diagnóstica e terapêutica do AVC isquêmico (AVCi) entre os médicos plantonistas do Hospital Regional de Conceição do Araguaia (HRCA) e entre estes e as evidências clínicas mais recentes para diagnóstico e tratamento de AVCi frente a dois casos clínicos. O presente estudo foi autorizado pelo HRCA e aprovado pelo Comitê de ética em Pesquisa Tapajós. As evidências científicas foram oriundas de diretrizes nacionais e internacionais localizadas através da busca nas bases de dados PUBMED, LILACS, EMBASE e COCHRANE LIBRARY. Após a assinatura do Termo de Consentimento Livre e Esclarecido (TCLE) pelos médicos plantonistas a pesquisa se desenvolveu por meio da aplicação de um questionário com dois casos clínicos hipotéticos que foram distribuídos aos profissionais médicos do setor de urgência e emergência. A variação na conduta diagnóstica e terapêutica foi identificada entre os profissionais e houve discordância da conduta destes com as diretrizes. Não indicar condutas recomendadas, e indicar condutas contraindicadas, pode representar risco á saúde e gastos desnecessários. A educação continuada deve ser incentivada para que a prática clínica seja baseada nas evidências científicas mais recentes. Como forma de contribuir para o diagnóstico e tratamento dos AVCi do serviço de urgência e emergência do hospital, as diretrizes guidelines for the Early Management of patientes With Acute Ischemic Stroke, guideline for acute ischemic stroke treatment - Part I, 2012 e guideline da AHA/ASA: Guidelines for acute ischemic stroke treatment – Part II: Stroke Treatment, 2012 foram disponibilizadas

An Overview of the TRP-Oxidative Stress Axis in Metabolic Syndrome: Insights for Novel Therapeutic Approaches

Metabolic syndrome (MS) is a complex pathology characterized by visceral adiposity, insulin resistance, arterial hypertension, and dyslipidaemia. It has become a global epidemic associated with increased consumption of high-calorie, low-fibre food and sedentary habits. Some of its underlying mechanisms have been identified, with hypoadiponectinemia, inflammation and oxidative stress as important factors for MS establishment and progression. Alterations in adipokine levels may favour glucotoxicity and lipotoxicity which, in turn, contribute to inflammation and cellular stress responses within the adipose, pancreatic and liver tissues, in addition to hepatic steatosis. The multiple mechanisms of MS make its clinical management difficult, involving both non-pharmacological and pharmacological interventions. Transient receptor potential (TRP) channels are non-selective calcium channels involved in a plethora of physiological events, including energy balance, inflammation and oxidative stress. Evidence from animal models of disease has contributed to identify their specific contributions to MS and may help to tailor clinical trials for the disease. In this context, the oxidative stress sensors TRPV1, TRPA1 and TRPC5, play major roles in regulating inflammatory responses, thermogenesis and energy expenditure. Here, the interplay between these TRP channels and oxidative stress in MS is discussed in the light of novel therapies to treat this syndrome

Redox Status of Postmenopausal Women with Single or Multiple Cardiometabolic Diseases Has a Similar Response to Mat Pilates Training

Postmenopausal women have a high prevalence of cardiometabolic diseases and that may associate with higher oxidative stress. Exercise can contribute to the treatment of such diseases, but some modalities, such as Mat Pilates, need to be further studied in terms of their physiological responses. Our aim was to investigate the effects of 12 weeks of Mat Pilates on redox status in postmenopausal women with one or multiple comorbidities of cardiometabolic diseases. Forty-four postmenopausal women were divided into two groups: SINGLE, composed of women with one cardiometabolic disease (n = 20) and MULT, with multimorbidity (n = 24). Mat Pilates training was conducted three times a week for 12 weeks, and each session lasted 50 min. Plasma samples were collected before and after training to analyze the following redox markers: superoxide dismutase, catalase, glutathione peroxidase, total antioxidant capacity due to ferric-reducing antioxidant power (FRAP), reduced glutathione (GSH), uric acid, and carbonyl protein. ANCOVA showed interaction effects in FRAP (p = 0.014). Both groups had reduced levels of catalase (p = 0.240) and GSH (p = 0.309), and increased levels of carbonyl protein (p = 0.053) after intervention. In conclusion, the redox status of postmenopausal women shows no changes mediated by Mat Pilates training between SINGLE and MULT, except for greater reductions of FRAP in SINGLE

Cuminaldehyde potentiates the antimicrobial actions of ciprofloxacin against Staphylococcus aureus and Escherichia coli.

Escherichia coli and Staphylococcus aureus are important agents of urinary tract infections that can often evolve to severe infections. The rise of antibiotic-resistant strains has driven the search for novel therapies to replace the use or act as adjuvants of antibiotics. In this context, plant-derived compounds have been widely investigated. Cuminaldehyde is suggested as the major antimicrobial compound of the cumin seed essential oil. However, this effect is not fully understood. Herein, we investigated the in silico and in vitro activities of cuminaldehyde, as well as its ability to potentiate ciprofloxacin effects against S. aureus and E. coli. In silico analyses were performed by using different computational tools. The PASS online and SwissADME programmes were used for the prediction of biological activities and oral bioavailability of cuminaldehyde. For analysis of the possible toxic effects and the theoretical pharmacokinetic parameters of the compound, the Osiris, SwissADME and PROTOX programmes were used. Estimations of cuminaldehyde gastrointestinal absorption, blood brain barrier permeability and skin permeation by using SwissADME; and drug likeness and score by using Osiris, were also evaluated The in vitro antimicrobial effects of cuminaldehyde were determined by using microdilution, biofilm formation and time-kill assays. In silico analysis indicated that cuminaldehyde may act as an antimicrobial and as a membrane permeability enhancer. It was suggested to be highly absorbable by the gastrointestinal tract and likely to cross the blood brain barrier. Also, irritative and harmful effects were predicted for cuminaldehyde if swallowed at its LD50. Good oral bioavailability and drug score were also found for this compound. Cuminaldehyde presented antimicrobial and anti-biofilm effects against S. aureus and E. coli.. When co-incubated with ciprofloxacin, it enhanced the antibiotic antimicrobial and anti-biofilm actions. We suggest that cuminaldehyde may be useful as an adjuvant therapy to ciprofloxacin in S. aureus and E. coli-induced infections

Monitoring of Peripheral Blood Leukocytes and Plasma Samples: A Pilot Study to Examine Treatment Response to Leflunomide in Rheumatoid Arthritis

Rheumatoid arthritis (RA) is a painful inflammatory disease of the joints which affects a considerable proportion of the world population, mostly women. If not adequately treated, RA patients can become permanently disabled. Importantly, not all the patients respond to the available anti-rheumatic therapies, which also present diverse side effects. In this context, monitoring of treatment response is pivotal to avoid unnecessary side effects and costs towards an ineffective therapy. Herein, we performed a pilot study to investigate the potential use of flow cytometry and attenuated total reflection–Fourier transform infrared (ATR-FTIR) spectroscopy as measures to identify responders and non-responders to leflunomide, a disease-modifying drug used in the treatment of RA patients. The evaluation of peripheral blood CD62L+ polymorphonuclear cell numbers and ATR-FTIR vibrational modes in plasma were able to discriminate responders to leflunomide (LFN) three-months after therapy has started. Overall, the results indicate that both flow cytometry and ATR-FTIR can potentially be employed as additional measures to monitor early treatment response to LFN in RA patients