HPLC and spectrophotometric investigation of the formation of Sn(II)-p-aminohippuric acid complex in perchlorate medium

The complex equilibrium in tin(II)-p-aminohippuric acid (PAH) solutions in perchlorate medium was investigated. HPLC, spectrophotometric and pH-metric methods were used. The experimental results have shown that week mononuclear complex of the general composition PAH/Sn(II)=1/1 was formed.Physical chemistry 2004 : 7th international conference on fundamental and applied aspects of physical chemistry; Belgrade (Serbia); 21-23 September 200

HPLC analysis of technetium(I)-99m labelled C60(OH)22

Fullerenols, water-soluble polihydroxylated fullerenes, are very important kinds of fullerene derivatives because it is suitable for biological study. In order to get convenient substance for studies (in vivo and in vitro) we investigate possibilities of labeling fullerenol. The HPLC results performed by isocratic HPLC, confirmed that hydrophilic organometallic [99mTc(CO)3(H2O)3]+ precursor allows forming of 99mTc(I) complexes with fullerenol.Physical chemistry 2004 : 7th international conference on fundamental and applied aspects of physical chemistry; Belgrade (Serbia); 21-23 September 200

Physico-chemical characterization of 90Y-labeled antimony trisulfide colloid and comparison with 99mTc-labeled one

In radionuclide therapy, the importance of 90Y as a beta-emitting radionuclide is increasing rapidly. The properties of the 90Y-labeled antimony trisulfide colloid (Sb2S3) were compared with the 99mTc-labeled one. Labeling efficiencies reached >96% and >97% for 90Y- and 99mTc-labeled colloids respectively. Both preparations were stable for 72 h in saline and 1% albumin solution. Filtration analysis showed that more than 94% of total 90Y radioactivity is associated with the colloidal particles smaller than 20 nm, while more than 90% of 99mTc radioactivity is associated with the particles retained on the filter with a 20 nm pore size. 90Y-labeled colloids showed high labeling efficiency, stability and potency for clinical use.Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry; Belgrade (Serbia); 24-28 September 200

Tricarbonyltechnetium (I) labelled ligands with nso donor atom set: in vitro and in vivo evaluation

There is an increasing interest for the 99mTc labelling of biomolecules by using bifunctional chelating agents. To find new ligand, which can be linked to the small biomolecules and coordinated with technetium-99m tricarbonyl complexes, is a challenging task. The investigated NSC and NSC5 ligands allow the preparation 99mTc(I) stable complexes in high yield. The 99mTc complexes were characterized by comparing their HPLC profiles with those of the respective Re(I) compounds. Biodistribution and stability studies were carried out, including challenge with histidine. These complexes also proved to be stable in vivo and showed a very good biological behaviour. The radiochemical and biological features of the novel 99mTc complexes, as well as, the nature of the ligands, make them very promising candidates for labelling of tumour specific biomolecules.Physical chemistry 2006 : 8th international conference on fundamental and applied aspects of physical chemistry; Belgrade (Serbia); 26-29 September 200

Investigating an organ-targeting platform based on hydroxyapatite nanoparticles using a novel in situ method of radioactive 125Iodine labeling

In this study, we have investigated the synthesis of nanoparticles of hydroxyapatite (HAp) and hydroxyapatite coated with chitosan (HAp/Ch) and the chitosan-poly-d,l-lactide-co-glycolide polymer blend (HAp/Ch-PLGA) as an organ-targeting system. We have examined and defined the final destination, as well as the dynamics and the pathways of the synthesized particles following intravenous administration in vivo.\ud \ud The XRD, ZP, FT-IR and SEM analyses have confirmed that the hydroxyapatite nanoparticles with d50 = 72 nm are coated with polymers. Radioactive 125-Iodine (125I), a low energy gamma emitter, was used to develop a novel in situ method for the radiolabeling of particles and investigation of their biodistribution. 125I-labeled particles exhibited high stability in saline and serum over the second day, which justified their use in the following in vivo studies.\ud \ud The biodistribution of 125I-labeled particles after intravenous injection in rats differed significantly: HAp particles mostly targeted the liver, HAp/Ch the spleen and the liver, while HAp/Ch-PLGA targeted the lungs. Twenty-four hours post injection, HAp particles were excreted completely, while both 125I-HAp/Ch and 125I-HAp/Ch-PLGA were retained in the body for a prolonged period of time with more than 20% of radioactivity still found in different organs

Factors affecting labelling yield of 111In-DTPA-BSA

Radiolabelling of antibodies depends on a number of factors including the chemical characteristics of the nuclide and the techniques employed for its incorporation into protein. For preliminary research we used model system and investigate the influence of different factors affecting labelling. Obtained results were successfully used for further radiolabelling of antibodies with different trivalent metals.Physical chemistry 2006 : 8th international conference on fundamental and applied aspects of physical chemistry; Belgrade (Serbia); 26-29 September 200

Radiochemical purity and particles number determinations of modified Tc-99m-macroaggregated albumin

A new procedure for the aggregation of human albumin and Tc-99m-labelling of the prepared macroaggregated albumin are presented. Simple methods for quantifying of all the radiochemical impurities existing in Tc-99m-MAA were tested. Thus, 85% methanol was used as the mobile phase in paper and ITL chromatography with Whatman N-o 1 and ITLC-SA strips. A system of two solvents (acetone and 1 M NaCl or 0.9% NaCl) was used for 3 MM paper, ITLC-SA and ITLC-SG strips and silica gel plates as the stationary phase. Low-voltage paper electrophoresis with Whatman 3 MM paper sheets soaked in barbiturate buffer and the gel chromatography column method (Sephadex G-25) were also applied. Filtration through syringe filters, proposed by European and Yugoslav Pharmacopoeia, was performed for comparison. The application of the mentioned tests lead to consistent results for the labelling efficiency ( GT 98.5%) and percent radiochemical impurities of Tc-99m-MAA. Determination of the particles number in a counter chamber and their size distribution under a light microscope with a calibrated ocular scale gave the result of 300000-350000 particles per 1 mg of HA. This confirmed that the human albumin macroaggregates prepared by our new procedure is remarkably improved and convenient for routine diagnostic purposes

99mTc-hexakis-(2-metoxy-isobutyl-isonitrile) ( 99mTc-MIBI) a new myocardial imaging agent: synthesis of MIBI, optimising conditions for radiolabelling with 99mTc at high radiochemical purity and in vivo behavior

99mTc-MIBI is a promising radiopharmaceutical for myocardial perfusion imaging agent, but it has also shown good results in identifying several types of tumors, such as breast, lung and thyroid cancers. It is a lipophilic, cationic technetium (1) complex. In this paper a complete study on the synthesis of 2-metohy-isobutyl-isonitrile (MIBI) as well as a formulation of a lyophilized kit for labeling with 99mTc is presented. Investigation on effective factors as well as finding out the optimum parameters to obtain the highest labelling efficiency and radiochemical purity of 99mTc-MIBI complex were performed. The radiochemical purity of the labelled preparation was high (>95%). Biodistribution study performed in health male Wistar rats showed satisfactory biokinetics results. 99mTc-MIBI was accumulated in sufficient amount into the hearth tissue for myocardial perfusion imaging. MIBI in kit formulation was found to be stable and also safe for administration.Physical chemistry 2006 : 8th international conference on fundamental and applied aspects of physical chemistry; Belgrade (Serbia); 26-29 September 200

99MtC(I)-tricarbonyl labeling of ethylene Duamine-N,N'-DI-3-Propanoate diethyl ester as potential radiopharmaceutical agent

There is an increasing interest for the 99mTc labeling of biomolecules by using bifunctional chelating agents like ethylenediamine-N,N´-di-3-propanoate diethyl ester (deeddp). To find new ligand, which can be linked to the small biomolecules and coordinated with [99mTc(CO)3(H2O)3] + precursor, is a challenging task. Radiolabeling of deeddp with [99mTc(CO)3(H2O)3] + precursor, stability studies and biodistribution of formed complexes were carried out, including challenge with histidine. Radiochemical yield of 99mTc(I)-tricarbonyl-deeddp complexes was higher than 95%. These complexes were stable in vitro and showed a very good biological behavior. The radiochemical and biological features of the novel 99mTc(I)-complexes, as well as, the nature of the ligands, make them very promising candidates for labeling of tumor specific biomolecules.Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry; Belgrade (Serbia); 24-28 September 201

The analysis of 2,3-dicarboxypropane-1,1-diphosphonic acid-coated magnetite nanoparticles under an external magnetic field and their radiolabeling for possible theranostic applications

The advances in nanotechnology are directed towards the development of new theranostic agents based on magnetic nanoparticles that can be used for both cancer detection and treatment. In this study, 2,3-dicarboxypropane-1,1-diphosphonic acid-coated magnetite nanoparticles (Fe 3 O 4 -DPD MNPs) were evaluated for their theranostic application using different methods. The magnetic hyperthermia efficiency of the Fe 3 O 4 -DPD MNPs was investigated in saline solution with ionic strengths between 0.05 and 1.0 mol dm −3 . For a better understanding of hyperthermia, the behavior of Fe 3 O 4 -DPD MNPs under a non-alternating magnetic field was studied, and the transparency of the sample was measured. Furthermore, the radiotracer method using the radionuclides 99m Tc and 90 Y was applied as a reliable and powerful method for evaluating the in vivo behavior of a nanoprobe; a high radiolabeling yield (>93%), in vitro and in vivo stability of the radiolabeled nanoparticles and high heating effect were observed, thus paving the way for the possible theranostic applications of Fe 3 O 4 -DPD MNPs.This is the peer-reviewed version of the following article:Perić, M.; Radović, M.; Mirković, M. D.; Nikolić, A. S.; Iskrenović, P.; Janković, D.; Vranješ-Đurić, S. The Analysis of 2,3-Dicarboxypropane-1,1-Diphosphonic Acid-Coated Magnetite Nanoparticles under an External Magnetic Field and Their Radiolabeling for Possible Theranostic Applications. New Journal of Chemistry 2019, 43 (15), 5932–5939. [https://doi.org/10.1039/c8nj06478d]