The prognostic impact of EGFR, ErbB2 and MET gene amplification in human gastric carcinomas as measured by quantitative Real-Time PCR

Purpose: Identification of critical genes which play pivotal roles in controlling tumor growth and survival will establish the basis for developing therapeutic targets. In this study, we focused on frequencies of EGFR, ErbB2 and MET gene amplification in gastric cancer patients to develop personalized medicine to improve the treatment. Method: EGFR, ErbB2 and MET gene amplification, and mRNA expression were analyzed by the quantitative Real-Time PCR in paraffin-embedded samples from 115 patients with gastric cancer. Results: EGFR, ErbB2 and MET genes were amplified in 11.3 % (13/115), 6.1 % (7/115) and 19.1 % (22/115) of cancerous specimens, respectively. The correlation coefficient test clearly indicated that gene amplification in these three genes was positively correlated with mRNA transcription (EGFR: R = 0.631, p = 0.009; ErbB2: R = 0.652, p = 0.023; MET: R = 0.715, p < 0.001). EGFR and MET gene amplification was significantly associated with Ki-67 MI (p = 0.022 and p = 0.015). MET amplification was also significantly associated with age of ≥60 years (p = 0.021) and tumor size of ≥5 cm (p = 0.032). MET amplification, but not EGFR and ErbB2, was a significant prognostic factor in poor survival among patients with gastric cancer. Conclusions: EGFR, ErbB2 and MET genes are frequently amplified in gastric carcinoma. EGFR, ErbB2 and MET gene amplification is positively correlated with mRNA transcription. MET gene amplification correlates with a poor prognosis and poor survival in gastric carcinomas. © 2015, Springer-Verlag Berlin Heidelberg

Use of integrase-minus lentiviral vector for transient expression

Objective: Lentivirus-derived vectors are among the most promising viral vectors for gene therapy which is currently available, but their use in clinical practice is limited due to associated risk of insertional mutagenesis. Gene targeting is an ideal method for gene therapy, but it has low efficiency in comparison to viral vector methods. In this study, we are going to design and construct an integrase-minus lentiviral vector. This vector is suitable for transient expression of gene and gene targeting with viral vector. Materials and Methods: In this experimental study, three missense mutations were induced in the catalytic domain of Integrase gene in the pLP1 plasmid and resulted D64V, D116A and E152G changes in the amino acid sequence through site directed mutagenesis. The pLenti6.2-GW/EmGFP transfer vector, associated with native and mutated packaging mix, was transfected into 293T cell line. In order to titer the lentivirus stock, the viruses were harvested. Finally, the viruses transduced into COS-7 cell line to assess green fluorescent protein (GFP) gene expression by a fluorescence microscopy. Results: Recombinant and wild lentiviruses titer was about 5�8�10 6 transducing units/ ml in COS-7 cell line. The number of GFP-positive cells transduced with native viruses was decreased slightly during two weeks after viral transduction. In contrast, in the case of integrase-minus viruses, a dramatic decrease in the number of GFP positive cells was observed. Conclusion: This study was conducted to overcome the integration of lentiviral genome into a host genome. Nonintegrating lentiviral vectors can be used for transient gene expression and gene targeting if a Target gene cassette is placed in the lentivirus gene structure. This combination method decreases disadvantages of both processes, such as random integration of lentiviruses and low efficiency of gene targeting

How to manage perioperative patient flow during COVID-19 pandemic: a narrative review

The COVID-19 pandemic due to the novel Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV2) has affected many aspects of the health system. One of those groups that are at very high risk are those health care personnel (including physicians, nurses, and managerial personnel) delivering the perioperative care; including preoperative care, intraoperative care and postoperative care. This is the same challenge for the patients who need to be admitted in hospital while they are feared of being affected by SARS-CoV2. This review considers the safety precautions for perioperative protection of healthcare personnel and patients based on the available evidence

Association between hair dye use and cancer in women: a systematic review and meta-analysis of case-control studies

Background: The use of hair dye for cosmetic purposes appears to be increasing worldwide. As 50-80% of women use hair dye throughout their lifetimes, the possible association between hair dye use and cancer is a public health concern. Method: This systematic review was performed by retrieving studies from PubMed, Scopus, WOS, and ProQuest databases. The inclusion criteria were case-control studies evaluating the association between hair dye use and cancer in women. Women with cancer who have used any hair dye were the focus of our study. Results: The present study combined 28 studies, to assess the association between hair dye use and cancer. The pooled odds ratio (OR) of hematopoietic system cancers among those who have generally ever used any type of hair dyes was 1.10 (95% CI:1.01-1.20) in 17 studies. In 11 studies investigating hair dye made before and after 1980 as a risk factor for cancer, the pooled OR for cancer was 1.31(95% CI:1.08-1.59). Likewise, in the 13 studies that evaluated the association of light and dark hair dye with cancer, the risk among those using dark hair dye increased by 9%, compared to non-users (OR=1.09; 95% CI:0.95-1.25). Conclusion: The present study suggests that, although the use of hair dye may increase the risk of cancer among users, a more detailed evaluation is required to assess the type of hair dye use in terms of guidelines and metrics. Keywords: Hair dye; cancer; meta-analysis

Juxta Cortical Tibia Metastatic Deposition in Gastric Cancer: A Case Report

We report a 41 years old man with rapidly growing and tender lump on the anteromedial surface of tibia. The patient had the history of gastrectomy and gastrojejunostomy due to gastric carcinoma. On admission, the Simple X-ray of lower extremity disclosed a slight thinning of the anterior cortex of tibia without cortical destruction. The whole-body bone scan with 99mTC MDP revealed activity of lesion in all 3 phases. The histopathological evaluation showed an infiltration of bone with tumor cells. Review No the literature revealed in previous cases of skeletal metastasis from gastric cancer in the tibia like this

Comparison of Religious Cognitive Behavioral Therapy, Cognitive Behavioral Therapy, and Citalopram on Depression and Anxiety among Women with Breast Cancer: A Study Protocol for a Randomized Controlled Trial

There has been evidence supporting the reduction of depression and anxiety by religious psychotherapy in cancer patients,however, there have been scarce randomized controlled trials. Therefore, there is a need for replication in a well-designedstudy to investigate the efficacy of these interventions among depressed women with breast cancer. A randomizedcontrolled trial is designed to be conducted on 160 women with breast cancer. Participants will be screened for anxietyand depression diagnosed by clinical interview based on the DSM-IV criteria and the Hospital Anxiety Depression Scale.Golriz and Baraheni’s Religious Attitude Questionnaire will be used to assess the religious attitude. Individuals meetingthe prerequisites will be randomly allocated to four groups, each containing 40 participants, including three interventiongroups (RCBT, CBT, and citalopram) and one control group (usual breast cancer treatment). An instrument by theEORTCQLQ-C30 and BR-23 will be used to measure the quality of life. Blood tests will be taken to assess biomarkers withthe (ELISA) method. The results will determine whether RCBT is more effective than other treatments. If so, the outcomesof the study will have implications not only for the management of similar problems in cancer patients but also for themanagement of other chronic diseases

The effect of therapeutic- supportive interventions on body image in women with breast cancer: A Systematic Review

زمینه و اهداف: مبتلایان به سرطان پستان دچار تصویر بدن منفی میشوند که بر ارتباط زناشویی تاثیر بسزایی دارد. مطالعات مرتبط متعددی وجود دارد که می توان با مطالعه ساختاریافته، از نتایج بهتر مطالعات مطلع شد، لذا هدف از مطالعه حاضر، تاثیر مداخلات درمانی - حمایتی بر تصویر بدن مبتلایان به سرطان پستان است. مواد و روشها : مطالعه حاضر یک مطالعه ساختاریافته است. با استفاده از کلید واژههای مرتبط و استراتژی بولی، پایگاههای اطلاعاتی G oogle Schoolar ،Web Of Science ،s cience Direct، C ochrane library، P roQuest، Scopus، PubMed از سال 1990 تا 2016 در مدت تیر تا شهریور 1395 مورد جستجو قرار گرفت. پس از اعمال معیار ورود و خروج، در نهایت 9 مطالعه وارد و با استفاده از مقیاس Jadad مورد بررسی و ارزیابی کیفیت قرار گرفتند. این مطالعه با کد طرح 95.224 و کد اخلاق به شماره IR.MAZUMS.REC.95.S.122 در کمیته دانشجویی ثبت شد یافته ها: از 9 مقاله، 6 مطالعه از نوع نیمه تجربی، 3 مطالعه کارآزمایی بالینی بود. نتایج 5 مطالعه نشان داد، مداخله سبب بهبود تصویر بدن بیماران شد. در مطالعه حاضر نتایج به دو طبقه پیامد اولیه &nbsp;)تاثیر مداخلات بر تصویر بدن ( و پیامد ثانویه )سن بیماران، ابزار سنجش، طول مدت پیگیری و مداخلات) سازماندهی گردید. ارزیابی مطالعات نشان داد روش ها نیاز نقطه قوت مقالات؛ شرح اهداف مطالعه، توضیح نتایج، معیار ورود - خروج و ضعف مطالعات در شرح کورسازی و ریزش نمونه ها بوده است نتیجه گیری: با توجه به اختلاف نظر بین مطالعات، مطالعه بیشتری با کارآمدترین روشها نیاز می باشد &nbsp;Background and Aims: Patients with breast cancer get negative perceptions of body image, which affects their marital relationships. There are a lot of related studies and, thus, it is possible to provide a complete, exhaustive summary of current literature relevant to these articles. Therefore, the aim of this study was to review the effects of therapeutic-supportive interventions on body image in patients with breast cancer using a systematic review design.Materials and Methods: In this systematic review study, Electronic databases including Google Scholar, Web of Sciences, Science Direct, Cochrane library, ProQuest, Scopus, and PubMed [including Medline]were searched for retrieving articles published between 1990 and 2016 during July to September 2016. After applying the inclusion and exclusion criteria, 9 studies remained for assessing their quality which were finally evaluated by using a Jadad scale.This study with the design code 224/95 and IR.MAZUMS.REC.95.S.122. was registered in the Student Committee.Results: Among 9 articles in this systematic review, 6 articles were of semi-experimental type and the remaining 3 articles were clinical trial studies. Results of 5 articles showed that intervention has improved body image in the patients. In this study, the results were organized on two-Category of primary outcome (impact of interventions on body image) and secondary outcome (age, assessment tool, duration of followup and treatment). Assessment of studies revealed that the objectives of studies, explaining of results, inclusion and exclusion criteria were the strength points of these articles. Describing of blinding and attrition were also the weaknesses points of these studies.Conclusion: Considering some disagreements between studies, further studies are needed with mostefficient ways

Perspektywa czasu u osób zdrowych i pacjentów cierpiących na raka lub cukrzycę

Introduction: Time orientation can significantly improve health-related prevention behaviour and influence disease outcome through boosting health-oriented behaviour. This study aims to compare time perspective between diabetic and cancerous patients, and healthy people. Methods: A cross-sectional descriptive study was conducted on 300 patients (105 healthy, 195 patients: 108 type 2 diabetes and 87 breast and digestive system cancer) aged 20 to 70 years (average age of 45.4 years). The samples were separated into three groups based on a convenience sampling method and were matched in terms of gender, age, education and monthly income. Data was collected through the Zimbardo Time Perspective Inventory (ZTPI-56) questionnaire and then evaluated with analysis of covariance (F test) followed by Fisher’s Least Significant Difference (LSD) test. Results: The three groups showed different time perspectives (F = 4.213, p < 0.05) and different ranking in time orientation. Conclusion: Our findings show that the disease and its type can significantly impact the time orientation of patients. Therefore, to prevent potential subsequent outcomes, the patients’ time perspective towards disease should be improved, especially under disease conditions