TMAG A930 (9 weeks)

Specimen A930 from Tasmanian Museum and Art Gallery, Tasmania, Australia. Specimen represents a single ~9 week old preserved pouch young. Zipped DiCOM stack with included reconstruction parameter

AMS P762 (12 weeks)

Specimen P762 from Australia Museum, New South Wales, Australia. Specimen represents a single ~12 week old preserved pouch young. Zipped DiCOM stack with included reconstruction parameter

TMAG A934 (non thylacine)

Misidentified pouch young specimen A934 from Tasmanian Museum and Art Gallery, Tasmania, Australia. Specimen is a Dasyurid pouch young, though the exact species unknown. Zipped DiCOM stack with included reconstruction parameter

TMAG A931 (5.25 weeks)

Specimen A931 from Tasmanian Museum and Art Gallery. Specimen represents a single ~5.25 week old individual from a litter of two preserved pouch young. Zipped DiCOM stack with included reconstruction parameter

Table_1_Self-reactivity of CD8 T-cell clones determines their differentiation status rather than their responsiveness in infections.xlsx

Mature T cells are selected for recognizing self-antigens with low to intermediate affinity in the thymus. Recently, the relative differences in self-reactivity among individual T-cell clones were appreciated as important factors regulating their fate and immune response, but the role of self-reactivity in T-cell biology is incompletely understood. We addressed the role of self-reactivity in T-cell diversity by generating an atlas of mouse peripheral CD8+ T cells, which revealed two unconventional populations of antigen-inexperienced T cells. In the next step, we examined the steady-state phenotype of monoclonal T cells with various levels of self-reactivity. Highly self-reactive clones preferentially differentiate into antigen-inexperienced memory-like cells, but do not form a population expressing type I interferon-induced genes, showing that these two subsets have unrelated origins. The functional comparison of naïve monoclonal CD8+ T cells specific to the identical model antigen did not show any correlation between the level of self-reactivity and the magnitude of the immune response.</p

DataSheet_1_Self-reactivity of CD8 T-cell clones determines their differentiation status rather than their responsiveness in infections.pdf

Mature T cells are selected for recognizing self-antigens with low to intermediate affinity in the thymus. Recently, the relative differences in self-reactivity among individual T-cell clones were appreciated as important factors regulating their fate and immune response, but the role of self-reactivity in T-cell biology is incompletely understood. We addressed the role of self-reactivity in T-cell diversity by generating an atlas of mouse peripheral CD8+ T cells, which revealed two unconventional populations of antigen-inexperienced T cells. In the next step, we examined the steady-state phenotype of monoclonal T cells with various levels of self-reactivity. Highly self-reactive clones preferentially differentiate into antigen-inexperienced memory-like cells, but do not form a population expressing type I interferon-induced genes, showing that these two subsets have unrelated origins. The functional comparison of naïve monoclonal CD8+ T cells specific to the identical model antigen did not show any correlation between the level of self-reactivity and the magnitude of the immune response.</p