NASYP: Online expert tool on the control of major-accident hazards involving dangerous substances

NASYP is an online Geoportal tool being developed in cooperation with state and regional authorities to improve insufficient practices based on implementation of Directive nr. 2003/105/ES on the control of major-accident hazards involving dangerous substances. The tool is applicable for managing the permits, reporting and regular monitoring issues. Furthermore, it’s applicable for a risk assessment and a rapid management of disasters in the initial phase. There’re simple modeling tools included to simulate early stages of the contamination caused by disasters occurred to be used for decision making and effective use of emergency services. In this manner, there’re low atmospheric and surface water pollutions taken into account. For the study area, Liberec region was chosen covering the area of 3,163km2 and containing 533 potentially dangerous objects categorized accordingly to the Directive nr. 2003/105/ES. The model simulations are responding to daily hydrological and meteorological situation, a capability of automated updates from databases operated by the Czech Hydro Meteorological Institute, and communicate with databases of substances operated by the regional authorities. NASYP is suitable especially for the “N†class of the operators defined in the Directive, where because of smaller amounts of stored dangerous substances the safety measures and regular inspections are limited.Spatial data, geoportal, risk management, modelling, Research and Development/Tech Change/Emerging Technologies, Research Methods/ Statistical Methods, Risk and Uncertainty, GA, IN,

Heterogeneity and Developmental Connections between Cell Types Inhabiting Teeth

Every tissue is composed of multiple cell types that are developmentally, evolutionary and functionally integrated into the unit we call an organ. Teeth, our organs for biting and mastication, are complex and made of many different cell types connected or disconnected in terms of their ontogeny. In general, epithelial and mesenchymal compartments represent the major framework of tooth formation. Thus, they give rise to the two most important matrix–producing populations: ameloblasts generating enamel and odontoblasts producing dentin. However, the real picture is far from this quite simplified view. Diverse pulp cells, the immune system, the vascular system, the innervation and cells organizing the dental follicle all interact, and jointly participate in transforming lifeless matrix into a functional organ that can sense and protect itself. Here we outline the heterogeneity of cell types that inhabit the tooth, and also provide a life history of the major populations. The mouse model system has been indispensable not only for the studies of cell lineages and heterogeneity, but also for the investigation of dental stem cells and tooth patterning during development. Finally, we briefly discuss the evolutionary aspects of cell type diversity and dental tissue integration

METHODS FOR KINEMATIC ANALYSIS OF HUMAN MOVEMENT IN MILITARY APPLICATIONS: A REVIEW OF CURRENT AND PROSPECTIVE METHODS

Expansion of methods employed in the kinematic analysis of human movement for diagnosing of the physical and mental health of subjects can be traced back to the 1990`s when new information technologies and electronic recording systems started their development boom. Evaluation methods of body movement for the diagnostics of physical and mental health expanded significantly in clinical practice. This study presents an overview of these methods with the focus on how applicable the analysis of human movement can be in military practice, where they are currently marginally used. The aim of this study is to offer some recommendations on how particular methods could be utilized in an army context. This article also suggests the most appropriate methods of quantitative evaluation for posture and motion control in the course of standing, gait and other activities carried out in military training and active duty

Candidate genes for obstructive sleep apnea in non-syndromic children with craniofacial dysmorphisms – a narrative review

Pediatric obstructive sleep apnea (POSA) is a complex disease with multifactorial etiopathogenesis. The presence of craniofacial dysmorphisms influencing the patency of the upper airway is considered a risk factor for POSA development. The craniofacial features associated with sleep-related breathing disorders (SRBD) – craniosynostosis, retrognathia and micrognathia, midface and maxillary hypoplasia – have high heritability and, in a less severe form, could be also found in non-syndromic children suffering from POSA. As genetic factors play a role in both POSA and craniofacial dysmorphisms, we hypothesize that some genes associated with specific craniofacial features that are involved in the development of the orofacial area may be also considered candidate genes for POSA. The genetic background of POSA in children is less explored than in adults; so far, only one genome-wide association study for POSA has been conducted; however, children with craniofacial disorders were excluded from that study. In this narrative review, we discuss syndromes that are commonly associated with severe craniofacial dysmorphisms and a high prevalence of sleep-related breathing disorders (SRBD), including POSA. We also summarized information about their genetic background and based on this, proposed 30 candidate genes for POSA affecting craniofacial development that may play a role in children with syndromes, and identified seven of these genes that were previously associated with craniofacial features risky for POSA development in non-syndromic children. The evidence-based approach supports the proposition that variants of these candidate genes could lead to POSA phenotype even in these children, and, thus, should be considered in future research in the general pediatric population

Heterogeneity and Developmental Connections between Cell Types Inhabiting Teeth

Every tissue is composed of multiple cell types that are developmentally, evolutionary and functionally integrated into the unit we call an organ. Teeth, our organs for biting and mastication, are complex and made of many different cell types connected or disconnected in terms of their ontogeny. In general, epithelial and mesenchymal compartments represent the major framework of tooth formation. Thus, they give rise to the two most important matrixproducing populations: ameloblasts generating enamel and odontoblasts producing dentin. However, the real picture is far from this quite simplified view. Diverse pulp cells, the immune system, the vascular system, the innervation and cells organizing the dental follicle all interact, and jointly participate in transforming lifeless matrix into a functional organ that can sense and protect itself. Here we outline the heterogeneity of cell types that inhabit the tooth, and also provide a life history of the major populations. The mouse model system has been indispensable not only for the studies of cell lineages and heterogeneity, but also for the investigation of dental stem cells and tooth patterning during development. Finally, we briefly discuss the evolutionary aspects of cell type diversity and dental tissue integration.(VLID)486225

NASYP: Online expert tool on the control of major-accident hazards involving dangerous substances

NASYP is an online Geoportal tool being developed in cooperation with state and regional authorities to improve insufficient practices based on implementation of Directive nr. 2003/105/ES on the control of major-accident hazards involving dangerous substances. The tool is applicable for managing the permits, reporting and regular monitoring issues. Furthermore, it’s applicable for a risk assessment and a rapid management of disasters in the initial phase. There’re simple modeling tools included to simulate early stages of the contamination caused by disasters occurred to be used for decision making and effective use of emergency services. In this manner, there’re low atmospheric and surface water pollutions taken into account. For the study area, Liberec region was chosen covering the area of 3,163km2 and containing 533 potentially dangerous objects categorized accordingly to the Directive nr. 2003/105/ES. The model simulations are responding to daily hydrological and meteorological situation, a capability of automated updates from databases operated by the Czech Hydro Meteorological Institute, and communicate with databases of substances operated by the regional authorities. NASYP is suitable especially for the “N” class of the operators defined in the Directive, where because of smaller amounts of stored dangerous substances the safety measures and regular inspections are limited

Generation and characterization of DSPP- Cerulean/DMP1-Cherry reporter mice

To gain a better understanding of the progression of progenitor cells in the odontoblast lineage, we have examined and characterized the expression of a series of GFP reporters during odontoblast differentiation. However, previously reported GFP reporters (pOBCol2.3-GFP, pOBCol3.6-GFP, and DMP1-GFP), similar to the endogenous proteins, are also expressed by bone-forming cells, which made it difficult to delineate the two cell types in various in vivo and in vitro studies. To overcome these difficulties we generated DSPP- Cerulean/DMP1-Cherry transgenic mice using a bacterial recombination strategy with the mouse BAC clone RP24-258g7. We have analyzed the temporal and spatial expression of both transgenes in tooth and bone in vivo and in vitro. This transgenic animal enabled us to visualize the interactions between odontoblasts and surrounding tissues including dental pulp, ameloblasts and cementoblasts. Our studies showed that DMP1-Cherry, similar to Dmp1, was expressed in functional and fully differentiated odontoblasts as well as osteoblasts, osteocytes and cementoblasts. Expression of DSPP-Cerulean transgene was limited to functional and fully differentiated odontoblasts and correlated with the expression of Dspp. This transgenic animal can help in the identification and isolation of odontoblasts at later stages of differentiation and help in better understanding of developmental disorders in dentin and odontoblast

Generation and characterization of DSPP‐Cerulean/DMP1‐Cherry reporter mice

To gain a better understanding of the progression of progenitor cells in the odontoblast lineage, we have examined and characterized the expression of a series of GFP reporters during odontoblast differentiation. However, previously reported GFP reporters (pOBCol2.3-GFP, pOBCol3.6-GFP, and DMP1-GFP), similar to the endogenous proteins, are also expressed by bone-forming cells, which made it difficult to delineate the two cell types in various in vivo and in vitro studies. To overcome these difficulties we generated DSPP- Cerulean/DMP1-Cherry transgenic mice using a bacterial recombination strategy with the mouse BAC clone RP24-258g7. We have analyzed the temporal and spatial expression of both transgenes in tooth and bone in vivo and in vitro. This transgenic animal enabled us to visualize the interactions between odontoblasts and surrounding tissues including dental pulp, ameloblasts and cementoblasts. Our studies showed that DMP1-Cherry, similar to Dmp1, was expressed in functional and fully differentiated odontoblasts as well as osteoblasts, osteocytes and cementoblasts. Expression of DSPP-Cerulean transgene was limited to functional and fully differentiated odontoblasts and correlated with the expression of Dspp. This transgenic animal can help in the identification and isolation of odontoblasts at later stages of differentiation and help in better understanding of developmental disorders in dentin and odontoblast