Dried-droplet probe preparation on AnchorChip targets for navigating the acquisition of matrix-assisted laser desorption/ionization time-of-flight spectra by fluorescence of matrix/analyte crystals.

We have developed a dried-droplet probe preparation method for peptide mass fingerprinting by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS), which uses AnchorChip targets and alpha-cyano-4-hydroxycinnamic acid (CHCA) as a matrix. Upon drying of a matrix and analyte mixture on the AnchorChip, salts and low molecular weight contaminants were pooled at the hydrophilic metal anchor, whereas 10-50 microm matrix/peptide crystals firmly adhered at the surface of a hydrophobic polymer and the entire target could be subsequently washed by submerging it in 5% formic acid for 2-3 min. Epifluorescence microscopy suggested that peptides were completely co-localized with CHCA crystals at the AnchorChip surface. Fluorescent images of the probes were of good contrast and were background-free, compared with images taken by a video camera built into the ion source. CHCA/peptide crystals were easy to recognize at the surface and peptide mass maps were acquired from them without further adjustment of the position of the laser beam. These crystals were remarkably stable towards the laser depletion and almost no matrix-related ions were typically observed in the low m/z region of peptide mass maps. The sensitivity of the peptide mass mapping was at the low-femtomole level

Swm1/Apc13 is an evolutionarily conserved subunit of the anaphase-promoting complex stabilizing the association of cdc16 and cdc27.

The anaphase-promoting complex (APC/C) is a large ubiquitin-protein ligase which controls progression through anaphase by triggering the degradation of cell cycle regulators such as securin and B-type cyclins. The APC/C is an unusually complex ligase containing at least 10 different, evolutionarily conserved components. In contrast to APC/C's role in cell cycle regulation little is known about the functions of individual subunits and how they might interact with each other. Here, we have analyzed Swm1/Apc13, a small subunit recently identified in the budding yeast complex. Database searches revealed proteins related to Swm1/Apc13 in various organisms including humans. Both the human and the fission yeast homologues are associated with APC/C subunits, and they complement the phenotype of an SWM1 deletion mutant of budding yeast. Swm1/Apc13 promotes the stable association with the APC/C of the essential subunits Cdc16 and Cdc27. Accordingly, Swm1/Apc13 is required for ubiquitin ligase activity in vitro and for the timely execution of APC/C-dependent cell cycle events in vivo

Phase-Noise Characterization in Stable Optical Frequency Transfer over Free Space and Fiber Link Testbeds

Time and frequency metrology depends on stable oscillators in both radio-frequency and optical domains. With the increased complexity of the highly precise oscillators also came the demand for delivering the oscillators’ harmonic signals between delocalized sites for comparison, aggregation, or other purposes. Besides the traditional optical fiber networks, free-space optical links present an alternative tool for disseminating stable sources’ output. We present a pilot experiment of phase-coherent optical frequency transfer using a free-space optical link testbed. The experiment performed on a 30 m long link demonstrates the phase-noise parameters in a free-space optical channel under atmospheric turbulence conditions, and it studies the impact of active MEMS mirror stabilization of the received optical wave positioning on the resulting transfer’s performance. Our results indicate that a well-configured MEMS mirror beam stabilization significantly enhances fractional frequency stability, achieving the−14th-order level for integration times over 30 s

Multi-purpose infrastructure for dissemination of precise stable optical frequency

Long distance precise frequency and accurate time transfer methods based on optical fiber links have evolved rapidly in recent years, demonstrating excellent performance. They are attractive both for very high-performance applications and as a secure alternative complement to radio- and satellite-based methods. In this paper, we present development of infrastructure for such transmission containing 700+km of transmission lines, with planned cross border optical frequency connectivity. According to our knowledge, this will be the third such line globally. The infrastructure also shares fibers with existing data transmissions, both amplitude and phase modulated, which poses high demands on mutual isolation and insensitivity to cross talks

Alternative spectral windows for photonic services distribution

Optical fibers are becoming commonly used beside data transmissions for dissemination of ultra-precise and stable quantities or alternatively as distributed sensors of for example acoustic and mechanic vibrations, seismic waves, temperature etc. There have been developed methods for these transfers and their stabilization, allowing thus to achieve excellent performances. Such performance is bound with utilization of single physical medium for both ways of propagation. These methods are attractive both for very high-performance applications and as a secure alternative complementary to radio and satellite-based transfer methods. From economical point of view, sharing fibers with regular data traffic is an advantage, especially for longer distances and large infrastructures. Unfortunately, the most often used wavelengths are located almost in the middle of telecommunication band. Due to continuous data traffic growth and utilization of flexible spectral allocation, the collision in wavelength plan will occur more and more often. In this paper we overview alternative wavelengths suitable for these transfers, we also propose suitable methods for all-optical reach extension, by all-optical amplification. Shared line design allowing transfer of ultra-stable quantities in three different spectral bands is proposed and such design is evaluated.</p

Alternative spectral window for precise time fiber based transport

Precise time and also stable frequency transfers are approaching the state to become almost standard applications for optical fiber networks, especially they are becoming supported in multiple research and education networks. In principle, the time and frequency transfers are not possible to be implemented over data transport layer, which hasn't been was designed for essential stability, and thus dedicated wave band is required for such implementation. To achieve even better stability for precise time and stable frequency, the bidirectional transmission in single physical medium (fibers and ideally all components along the route) is preferred. The White Rabbit system combines synchronous Ethernet and Precise Time Protocol (IEEE-1588) and it has been designed in the CERN with the aim to provide time synchronization among large number of sensors, actuators and other devices utilized in experiments. Primary aim was the operation over single fiber (to avoid nonreciprocal changes in directions) using standard telecommunication transceivers designed for single fiber operation with reach limited to about 40 km. In this paper, we experimentally verify and compare stability of White Rabbit system operation over long distances up to 300 km using active optical amplification on wavelengths which should not be utilized within spectrum needed for regular data transmissions.</p