Influence of salinity upon growth rate and tolerance of Barbus sharpeyi

Studies show that there are some freshwater fishes which can survive in brackish and saline waters. Although it is not long that study on the tolerance of fishes against various salinities and effect of salinity on growth rate has been caught up in scientific fields and that only a few fishes have been subjected to such studies, promising results encouraged us to carry out similar experiments on Barbus sharpeyi from cyprinidae Freshwater Barbus sharpeyi transferred to brackish water with different salinity degrees in three stages as follows : 1) Direct transfer from freshwater to waters of 2, 4, 6, ... , 16, 18 ppt salinity 2) Stepwise transfer to waters of 1, 2 3,...., 17, 18 ppt salinity for tolerance studies 3) Stepwise transfer to waters with two degrees difference in salinity, for growth rate studies. In this phase freshwater was taken as control. Statistical analysis Computer analysis of acquired data was carried out using t-student and ANOVA statistic, Analyses were significant at p<0.05

Hydrocarbons quality survey in the water, sediment and bivalve in the north-eastern of the Persian Gulf

Monthly samples of the bivalve Pinctada fucata were collected within one year (1995-96) in the north-eastern (Nakhiloo, Hendurabi and Lavan) of the Persian Gulf. The objective of this study was hydrocarbons quality survey. Thy above regions are natural habitant of pearl oyster (P.fucata). The primary sampling showed that pearl oyster stocks decreased in Lavan and Hendurabi regions, therefore the regions were selected as experimental stations. The oil hydrocarbons were measured in water (surface and depth), sediment and bivalves. The result indicated that in the north of Lavan, due to few currents of water hydrocarbons' concentration was more than north eastern part of Lavan, Hendurabi and Nakhiloo regions and in the, Nakhiloo region, in which hydrocarbons were less than studied stations, the bivalve size were larger

Evaluation of the antioxidant activity of dried (rehydrate) and fresh sea cucumber, Holothuria parava

Sea cucumber is a valuable resource containing several materials that can be used as natural products and pharmaceutical industries. In this study, proximate composition and the amount of total phenols, flavonoids and antioxidant activity of fresh and rehydrated (after drying) sea cucumber, Holothuria parva, were examined. The samples were collected from Bandar Lengeh in the Persian Gulf during low tide and kept frozen. The samples were freeze-dried and the moisture content was calculated. The protein, lipid, ash and carbohydrate contents were then measured. The amounts of antioxidant activity (DPPH), phenol and flavonoid contents were also measured. All results have possibility (p<0.05). In fresh sample, the moisture, protein, lipid, ash and carbohydrate contents were 81.20, 12.00, 0.34, 5.50 and 0.95%, respectively, while they were 92.50, 3.76, 0.36, 1.00 and 2.07% in rehydrated sample. The antioxidant activity in fresh and rehydrated samples were equal to 0.063 and 0.060 mg vitamin C/g dry sample, respectively. The IC50 in fresh sample was 5.26 while in the rehydrated sample was 4.14. Total phenol content of fresh and rehydrated samples were equal to 0.22 and 0.19 mg gallic acid/g dry sample, repectively. The flavonoid contents in fresh and rehydrated samples were equal to 3.86 and 5.02 mg Quercetin/g dry sample, respectively. The amounts of protein and ash in fresh sample were significantly higher than rehydrated sample with significant difference (p<0.05). Moisture in rehydrated sample was significantly higher than fresh sample with significant difference (p<0.05). There were no significant difference between fresh and rehydrated samples in terms of their phenon and flavonoid contents. The DPPH radical scavenging capacity was significantly higher in the fresh sample compared to the rehydrated sample with significant difference(p<0.05). It can be concluded that this species of fresh sea cucumber has more antioxidant activity than rehydrated (after drying) sea cucumber and more beneficial to be used in pharmaceutical and food industries

Study on some hematological factors of Salmo trutta caspius

Regarding to measure some hematological factors of Caspian Salmon (Salmo Trutta caspius), 55 immature and 21 mature individuals were studied. The main results were: Immature Mature - Hemoglobin value (gr/dl) 7.20 9.87 - Hematocrit 34.1 39.2 - Mean No. of red blood cells (per mm3) 934300 1306200 - Mean No. of white blood (per mm3) 8380 6219 - Mean No. of platelets (per mm3) 21180 13159 As the results showed the number of white blood cells and platelets in immature individuals were more than mature specimen. The obtained results of blood indices were as follow: Immature Mature - Mean MCV (FL) 385.4 302.9 - Mean MCH (Pg) 82.0 75.5 - Mean MCHC (%) 21.6 25.6 - Lymphocyte (%) 96.1 69.9 - Neutrophil (%) 3.4 29.4 - Monocyte (%) 0.52 1.14 Notice: Eosinophil and basophil cells weren't observed

Extraction and identification sterols in brown alga, Padina boergesenii in Chabahar Coasts

Padina boergesenii is one of the most abundant brown algae distributed in the north of Persian Gulf and Oman Sea. In this study after sampling and preparation of Padina boergesenii by Chroform-Etanol (3-1) solvent and by Methanol has been extract. Separation and purification of the compounds was carried out using thin layer, general and inverse column chromatography, Cephadex and high-performance liquid chromatography (HPLC). Structural elucidation of the constituents was based on the data obtained from H-NMR, 13C-NMR, HSQC, HMBC, DEPT and Cephadex LH-20. The steroids compounds separated from above alga were identified as 22-dehydrocholesterol (1), cholesterol (2), fucosterol (3), β-sitosterol (4), stigmasterol (5), ostreasterol (6) and two epimer of hyroxyestrol (7), based on their spectral data and from comparison with those previously reported in the literature

Effect of malathion insecticide on liver tissue and enzymes of Rutilus rutilus caspicus of the Caspian Sea

Malathion is an organophosphate insecticide which uses to destroy insects and pests of fruit trees, ornamental plants and agricultural corps. In the present study, effect of Malathion on liver and selected enzymes (SGOT, SGPT and ALP) was studied in Caspian Roach (Rutilus rutilus caspicus). Four treatments with three replications were designed to carry out the survey. Four groups of experimental fish (containing 30 fish in each group) were exposed to different concentrations of Malathion. e. 0, 0.01, 0.05 and 0.1 ppm respectively for 23 days. Blood collection was done in 3rd, 13th and 23th after exposure to Malathion and also 30 days after recovery in clean water and enzymes were measured using standard kits. Also liver tissues were isolated to histological examination. Results showed that tissues of control group (0ppm) were normal and there were no damages, yet there were hepatocytes degeneration, picnotic in nuclear, hepatocytes vacuolization, vascular congestion and sinusoid congestion in liver of other groups. Tissue damages were increased in higher malathion concentration and over time. Results related to enzymes showed that there were no significant differences in SGOT of fish treated with low concentrations of malathion (0.01 and 0.05 ppm) and control group but it was increased in highest concentration (p<0.05). Yet, SGPT increased significantly after passing 23 days in all fish exposed to malathion But ALP changes trend was decreasing

The effect of thyroxine on percentage of hatching (Hypophthalmichthys molitrix)

Studies showed that Thyroxine can play an important role on fish growth, osmoregulation system, and other physiological activities. Since the direct effect of thyroxine on growth and metamorphose of bony fish larvae has not been yet approved, therefore, present study were carried out in Shahid Ansary propagation and cultivation farm in order to find out the way of effect of this hormone on the survirval rate and early growth stage of silver carp (Hypophthalmichthys molitrix) fertiled eggs that were bathed in thyroxin with concentrations of 0.1, 0.5 and 1.0 ppm (each one, replicates) for 6 hours. Then they were transfered into large incubators (zoog) with running water. After hatching all larvae were placed in mentioned thyroxin concentration bath for another 6 hours before being transfered into large incubators. Larvae stayed inzoogs until the yolk-sac was absorbed. Finally, live silver carp larvae were counted in separated plates. Results of present study indicated that the ra tio of hatched eggs in 0.5 ppm of thyroxin concentration was higher than other treatment

Investigation of antibacterial and antifungal activities of the extract marine algae Sargassum glaucescens

In recent decades the use of algae in the food and pharmaceutical industries is of great importance. The antimicrobial activity of brown alga methanol, ethyl acetate, hexane, and chloroform extracts on bacteria gram positive, gram negative, and fungi was evaluated by using nutrient broth macrodilution test. Sargassum glaucescense was collected around the coastal waters of Chabahar (Oman Sea) in Nov and Dec 2015. Six pathogenic organisms including; Enterococcus faecium ATCC 51299, Streptococcus mutans ATCC 35668, Shigella boydii ATCC 25923, Pseudomonas aeruginosa ATCC 27853, Klebsiella pneumoniae ATCC 13883, Salmonella enteritidi PTCC, 1709, Candida albicans ATCC 10231 and Aspergillus fumigatus PTCC 5009 were investigated by the broth dilution method. Methanolic Extract for six strains showed good activity amongst eight strains. Hexane extract, after methanolic extract has good effect on antimicrobial activity against five strains. All bacteria strain in this survey has showed resistance against ethyl acetate and chloroformic extracts. All extract of S. glaucescens has good inhibition growth against two fungal strains. S. glaucescens using four various solution extract against eight different human pathogens showed an important antimicrobial and antifungal activity. However, more investigation has to be done on separation, purification and detection of the active ingredients in order to recognize their antifungal and antibacterial activity

Community composition and diversity of zooplankton in the northwest Persian Gulf

Survey on zooplankton composition was studied during June 2015 to March 2016, by selecting six stations along the coastal waters of Bushehr (the Northwest Persian Gulf). Twenty four zooplankton taxa were identified, and the different zooplankton groups represented twenty-one families. Copepoda was recorded with the most abundance (53.30 %) followed by Malacostraca (32.87 %), which in turn was followed by Sagittoidea (7.44 %) and Appendicularia (6.39%). A major peak of 189.34 N/m3 was observed in February-2016 with 53.25% contribution from Copepoda. Among Copepoda, Labidocera sp. was the major contributor to this peak. Appendicularia was the comparatively less represented group, being chiefly represented by Oikopleura dioica. Labidocera sp., Oithona plumifera which were common in most of the stations. This common distribution were observed for Malacostraca, namely Lucifer hanseni (mysis I), Upogebia sp. (zoea I), Parthenope sp., Ilyoplax frater( zoea VI), for Sagittoidea, namely Sagitta enflata, Sagitta neglecta and for Appendicularia, namely Oikopleura dioica. The mean Shannon's diversity index (H') and evenness were 1.36±0.43 and, 0.68±1.17, respectively. The highest Margalef's index was recorded in station-6 (2.72±1.32) and the lowest in station-2 (1.98±0.89). According to non-significant differences between temporal and spatial zooplankton density and Shannon’s index, from an ecological point of view, it seems the study area is unique and the zooplankton composition is homogenous

Analysis of vitellogenin gene structure in Caspian roach, Rutilus caspicus (Pisces: Cyprinidae) during exposure to Atrazine

Chemical contamination of aquatic environments to EDCs has become a major focus of environmental toxicology research. The exposure of fishes to estrogenic EDCs in aquatic environments is most frequently assessed by analyzing Vitellogenin (Vg) (the egg yolk precursor protein) expression. Therefore, characterization of Vg gene is of high priority for EDCs bio-monitoring. So, we prepared liver tissue samples of Caspian roach, Rutilus caspicus for RNA extraction. Following the cDNA synthesis, specifically - designed primers were employed to amplify the Vg gene and ultimately sequence it. The evolutionary analyses of the sequence were performed using MEGA7 software. The obtained results indicated that the designed primers successfully amplified the partial cDNA sequence. Our results indicated that this sequence most probably belongs to the Vg1 form of the gene. Moreover, it was demonstrated that Caspian roach and Petroleuciscus esfahani share a common ancestor. Noteworthy, the study of Vg gene would be helpful to understand the molecular mechanisms of development and would be used to establish a bio-monitoring tool for detection of exposure to different EDCs