Purification of antibacterial compounds from spathiphyllum cannifolium leaf

Spathiphyllum cannifolium is an ‘araceae’ species which can be found in Malaysia for landscaping. Previous study carried out in this lab found that this plant possesses the highest antibacterial activity among 19 flowering plants screened. In the present study, purification of antibacterial compound of the plant was conducted. For purification of antibacterial compound, open column chromatography was conducted and ethyl acetate, hexane and ethanol are used as the mobile phase. The results showed that the elution of column with 80% ethyl acetate in ethanol provide fractions with the highest antibacterial activity. The fractions were analyzed with TLC plates and revealed a single spot under UV light at 0.85 Rf value. Further purification of the single spot using HPLC showed two isolated compounds at retention time of 5.53 minutes and 8.26 minutes were obtained. The compounds were suspected as flavanoids as they can be detected at wavelength 360 nm and 400 nm

Muslims in South Korea: The way of life / Mahsuri Khalid … [et al.]

The purpose of this study is to understand the life of Muslims in South Korea. It focuses on cultural and societal factors that may influence the quality of life of Muslims in South Korea. This study uses an interview with 25 respondents that represent both local Muslim Koreans and Muslim foreigners who live in South Korea. The data then has been analysed as a descriptive method that shows life as Muslims in Korea is quite challenging as they are still a minority and struggling with the Islamophobia issues. The findings may be useful to any social researches in identifying more variables and factors in shaping the roles that can be played by the Muslims community in South Korea

Media screening of lactic acid fermentation using Lactobacillus rhamnosus

rhamnosus) was successfully carried out in shake flask experiment. Initially eleven variables were screened using Placket Burman Design and the results indicated that, the main variables that affected the process were glucose and peptone. The correlation between those two variables was analyzed using Response Surface Methodology (RSM). The contour plot indicates that there is no significant interaction between these two variables

Influence of operating variables on the in-situ transesterification using CaO/Al2(SO4)3 derived from waste

The purpose of this research project is to investigate the effects of amount of catalyst, the ratio of solid to methanol and different temperature on the biodiesel yield. Solid coconut waste and heterogeneous catalyst derived from the waste, which is calcium oxide (CaO) are used to produce biodiesel and also examine the kinetics of the reactive extraction. In this project, the heterogeneous catalyst is produced by combining solid coconut waste and egg shells and finally treating it with Al2(SO4)3. Then, the mixture of wastes was calcinated under high temperature. Then, solid coconut waste in situ transesterification experiment were conducted at different amount of catalysts (2-6wt%), temperature (50-65°C) and methanol to oil ratio (6:1 – 12:1). The optimum parameters were the solid to methanol ratio, temperature and mixing speed which were at 1:10, 60 °C and 350 rpm respectively with highest yield of 47 wt%. The result proves that the biodiesel conversion increase with methanol, but the reaction did not change and decrease after achieving certain percentage of methanol

Purification and partial characterization of L-asparaginase enzyme produced by newly isolated Bacillus sp

A new bacterial producing L-asparaginase was successfully isolated from Sungai Klah Hot Spring, Perak, Malaysia and identified as Bacillus sp. It was the best L-asparaginase producer as compared to other isolates. Production of L-asparaginase from the microbial strain was carried out under liquid fermentation. The crude enzyme was then centrifuged and precipitated with ammonium sulfate before being further purified by chromatographic method. HiTrap DEAE-Sepharose Fast Flow ion exchange chromatography followed by separation on Superose 12 gel filtration were used to obtain pure enzyme. The purified enzyme showed 10.11 U/mg of specific activity, 50.07% yield with 2.21 fold purification. The purified enzyme was found to be dimer in form, with a molecular weight of 65 kDa as estimated by SDS-PAGE. The maximum activity of the purified L-asparaginase was observed at pH 9 and temperature of 60 oC. Kajian penyelidikan ini telah berjaya menghasilkan enzim L-aspraginase daripada bakteria-bakteria baru yang diambil dari Kolam Air Panas, Sungai Klah, Perak, Malaysia dan ia dikenal pasti sebagai Bacillus sp. Teknik isolasi bakteria ini adalah teknik terbaik dalam menghasilkan enzim L-asparaginase berbanding teknik-teknik lain. Penghasilan enzim ini dibuat menerusi proses fermentasi. Kultur bakteria yang diperolehi diempar dan diikuti presipitasi menggunakan ammonium sulfat sebelum proses penulenan seterusnya dilakukan menggunakan kaedah kromatografi. Kolum penukaran ion jenis HiTrap DEAE-Sepharose Fast Flow diikuti dengan pemisahan oleh gel Superose 12 telah digunakan untuk mendapatkan enzim L-asparaginase yang tulen. Hasil kajian mendapati enzim tulen yang diperolehi mempunyai aktiviti spesifik sebanyak 10.11 U/mg daripada 50.07% enzim yang dihasilkan dan berjaya mencapai 2.21 kali ganda tulen dari enzim tanpa proses penulenan. Enzim tulen yang diperolehi didapati dalam bentuk “dimer“ dengan berat molekular sebanyak 65 kDa yang ditentukan menerusi SDS-PAGE. Enzim ini juga menunjukkan aktiviti yang tinggi pada pH 9 dan suhu 60 oC

PURIFICATION AND PARTIAL CHARACTERIZATION OF L-ASPARAGINASE ENZYME PRODUCED BY NEWLY ISOLATE BACILLUS SP.

A newly bacterial producing L-asparaginase was successful isolated from Sungai Klah Hot Spring, Perak, Malaysia and identified as Bacillus sp. It was the best L-asparaginase producer as compared to other isolates. Production of L-asparaginase from the microbial strain was carried out under liquid fermentation. The crude enzyme was then centrifuged and precipitated with ammonium sulfate before further purified with chromatographic method. The ion exchange chromatography HiTrap DEAE-Sepharose Fast Flow column followed by separation on Superose 12 gel filtration were used to obtain pure enzyme. The purified enzyme showed 10.11 U/mg of specific activity, 50.07% yield with 2.21 fold purification. The purified enzyme was found to be dimer in form, with a molecular weight of 65 kDa as estimated by SDS-PAGE. The maximum activity of the purified L-asparaginase was observed at pH 9 and temperature of 60°C

Panus tigrinus as a potential biomass source for Reactive Blue decolorization: Isotherm and kinetic study

Background: Textile and dye industries pose a serious threat to the environment. Conventional methods used for dye treatment are generally not always effective and environmentally friendly. This drove attention of scores of researchers to investigate alternative methods for the biodegradation of dyes using fungal strains. In this work, white-rot fungus (Panus tigrinus) was used as a biosorbent for the decolorization of Reactive Blue 19. The process parameters that were varied were initial concentration (50\u2013150 mg/L), contact time (30\u201390 min), and pH (2\u20136). In addition, to gain important data for the evaluation of a sorption process, the equilibrium and kinetics of the process were determined. Results: White-rot fungus showed great potential in decolorizing Azo dyes. The strain showed the maximum decolorization of 83.18% at pH 2, a contact time of 90 min, and an initial concentration of 50 mg/L. The Langmuir isotherm described the uptake of the Reactive Blue 19 dye better than the Freundlich isotherm. Analysis of the kinetic data showed that the dye uptake process followed the pseudo second-order rate expression. Conclusion: The biosorption process provided vital information on the process parameters required to obtain the optimum level of dye removal. The isotherm study indicated the homogeneous distribution of active sites on the biomass surface, and the kinetic study suggested that chemisorption is the rate-limiting step that controlled the biosorption process. According to the obtained results, P. tigrinus biomass can be used effectively to decolorize textile dyes and tackle the pollution problems in the environment

The impact of cosmetic advertisements in television: a case study among students in Kolej Melati UiTM Shah Alam / Noraini Jamal

The purpose of this study is to study the impact of cosmetic advertisement in television among students in Kolej Melati. This research only involves female students and it is conducted at Kolej Melati,UiTM Shah Alam. The research applies Uses & Gratification Theory because it fit to the topic and relate to the variables. The researcher also use quantitative research as the methodology to conduct this study by use descriptive analysis which is the transformation ofraw data into a form so that it is easy to understand, interpret, to produce descriptive information. Besides that, the researcher distribute questionnaire to the 70 respondent that from variety of field of study. Statistical Package for the Social Science (SPSS) also helps the researcher to change the raw to significant data for this research

A review on 5S and total productive maintenance and impact of their implementation in industrial organizations

The key purpose of this paper is to show what has been written about 5S and Total Productive Maintenance (TPM), and the benefits that can be achieved by their implementation in the contemporary industrial organizations. The findings of this paper reveal important issues about the impact of the implementation of 5S in TPM projects, thereby toward improved effectiveness of performance of industrial organizations and enhance their financial position and competitively. The literature on the classification of 5S and Total Productive Maintenance (TPM) has so far been very limited. The paper reviews a large number of published materials in this field, along with it presents the overview about the basic concepts and various practices of implementation of the 5S and TPM. It also highlights many aspects which are needed for implementing these two techniques simultaneously in the contemporary manufacturing scenario. Moreover, in this work, the phases of the successful implementation of 5S and TPM have been highlighted for ensuring smooth and effective their implementation in the workplaces, in addition to identify the factors required to correctly implement the 5S and the TPM. Sum up, the paper contains a comprehensive listing of the leading publications and which have come a long way in the area of 5S and TPM, in addition to a set of proposals that have been developed based on the study model after extensive review of literature. Thus, this paper will be largely beneficial to researchers, maintenance professionals and other concerned officials with improving the performance of production process effectiveness in the industrial organizations. © 2015 American Scientific Publishers. All rights reserved