Industrially produced enriched sterilized milk drinks for children nutrition

Nepravilna ishrana bogata mastima i ugljikohidratima, a siromašna u biološki visokovrijednim sastojcima hrane, prisutna je odavno i još uvijek u našoj zemlji. Ova ispitivanja su izvedena sa ciljem dobivanja zdrave hrane na bazi mlijeka za djecu, obogaćene proteinima, vitaminima i mineralnim tvarima. U industrijskim uvjetima su uspješno proizvedeni sterilizirani mliječni napici: mlijeko, čokoladni i karamel napitak, obogaćeni Na-kazeinatom, vitaminom A i željezom. Fizičko-kemijska, mikrobiološka i organoleptička kvaliteta proizvoda je kontrolirana tokom tromjesečnog skladištenja.The irregular nutrition, rich in fats and carbohydrates, and poor in biologically highly valuable food components, is present from long ago up to now in our country. These investigations were carried out in the aim of obtaining dairy based healthy foods for children, enriched in proteins, vitamins and minerals. Sterilized dairy drinks with the taste of milk, chocolate and caramel, enriched with Na-caseinate, vitamin A and iron were successfully produced under industrial conditions. Physico-chemical, microbiological and organoleptical qualities of the products were controlled during the three months storage

Industrially produced enriched sterilized milk

Nepravilna ishrana bogata mastima i ugljenim hidratima, a siromašna u biološki visokovrijednim sastojcima hrane prisutna je još uvijek u našoj zemlji. Ova ispitivanja su izvedena sa ciljem dobivanja zdrave hrane na bazi mlijeka, obogaćene proteinima, vitaminima i mineralnim tvarima. U industrijskim uvjetima su uspješno proizvedena sterilizirana mlijeka obogaćena Na-kazeinatom, vitaminom A, D i željezom. Fizičko-kemijska, mikrobiološka i organoleptička kvaliteta proizvoda je kontrolirana tokom tromjesečnog skladištenja.The irregular nutrition, rich in fats and carbohydrates, and poor in biologically highly valuable food components, is still present in our country. These investigations were carried out in the aim of obtaining dairy based healthy foods enriched with proteins, vitamins and minerals. Sterilized milks enriched with Na-caseinate, vitamin AD and iron, were successfully produced under industrial conditions. Physico-chemical, microbiological and organoleptical qualities of the products were controlled during three months storage

Growth Effects of Some Platinum(II) Complexes with Sulfur-Containing Carrier Ligands on MCF7 Human Breast Cancer Cell Line upon Simultaneous Administration with Taxol

The platinum (II)complexes, cis-[PtCl2(CH3SCH2CH2SCH3)] (Pt1), cis-[PtCl2(dmso)2] (dmso is dimethylsulfoxide; Pt2) and cis-[PtCl2(NH3)2] (cisplatin), and taxol (T) have been tested at different equimolar concentrations. Cells were exposed to complexes for 2 h and left to recover in fresh medium for 24, 48 or 72 h. Growth inhibition was measured by tetrazolium WST1 assay Analyses of the cell cycle, and apoptosis were performed by flow cytometry, at the same exposure times. The IC50 value of each platinum(II) complex as well as combination index (CI; platinum(II) complex + taxol) for various cytotoxicity levels were determined by median effects analysis

Synthesis, characterization, HSA/DNA interactions and antitumor activity of new [Ru(η6-p-cymene)Cl2(L)] complexes

Three new ruthenium(II) complexes were synthesized from different substituted isothiazole ligands 5-(methylamino)-3-pyrrolidine-1-ylisothiazole-4-carbonitrile (1), 5-(methylamino)-3- (4-methylpiperazine-1-yl)isothiazole-4-carbonitrile (2) and 5-(methylamino)-3-morpholine-4- ylisothiazole-4-carbonitrile (3): [Ru(η6-p-cymene)Cl2(L1)]·H2O (4), [Ru(η6 -pcymene)Cl2(L2)] (5) and [Ru(η6-p-cymene)Cl2(L3)] (6). All complexes were characterized by IR, UV-Vis, NMR spectroscopy, and elemental analysis. The molecular structures of all ligands and complexes 4 and 6 were determined by an X-ray. The results of the interactions of CT-DNA (calf thymus deoxyribonucleic acid) and HSA (human serum albumin) with ruthenium (II) complexes reveal that complex 4 binds well to CT-DNA and HSA. Kinetic and thermodynamic parameters for the reaction between complex and HSA confirmed the associative mode of interaction. The results of Quantum mechanics (QM) modelling and docking experiments toward DNA dodecamer and HSA support the strongest binding of the complex 4 to DNA major groove, as well as its binding to IIa domain of HSA with the lowest ΔG energy, which agrees with the solution studies. The modified GOLD docking results are indicative for Ru(p-cymene)LCl··(HSA··GLU292) binding and GOLD/MOPAC(QM) docking/modelling of DNA/Ligand (Ru(II)-N(7)dG7) covalent binding. The cytotoxic activity of compounds was evaluated by MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl2H-tetrazolium bromide) assay. Neither of the tested compounds shows activity against a healthy MRC-5 cell line while the MCF-7 cell line is the most sensitive to all. Compounds 3, 4 and 5 were about two times more active than cisplatin, while the antiproliferative activity of 6 was almost the same as with cisplatin. Flow cytometry analysis showed the apoptotic death of the cells with a cell cycle arrest in the subG1 phase

Industrially produced enriched sterilized milk

Nepravilna ishrana bogata mastima i ugljenim hidratima, a siromašna u biološki visokovrijednim sastojcima hrane prisutna je još uvijek u našoj zemlji. Ova ispitivanja su izvedena sa ciljem dobivanja zdrave hrane na bazi mlijeka, obogaćene proteinima, vitaminima i mineralnim tvarima. U industrijskim uvjetima su uspješno proizvedena sterilizirana mlijeka obogaćena Na-kazeinatom, vitaminom A, D i željezom. Fizičko-kemijska, mikrobiološka i organoleptička kvaliteta proizvoda je kontrolirana tokom tromjesečnog skladištenja.The irregular nutrition, rich in fats and carbohydrates, and poor in biologically highly valuable food components, is still present in our country. These investigations were carried out in the aim of obtaining dairy based healthy foods enriched with proteins, vitamins and minerals. Sterilized milks enriched with Na-caseinate, vitamin AD and iron, were successfully produced under industrial conditions. Physico-chemical, microbiological and organoleptical qualities of the products were controlled during three months storage

Подобрување на сортните одлики на пиперката Куртовска капија по пат на одбирање и анализа на почетниот материјал

Од вкупните површини под пиперка во Република Македонија (9.000- 10.000) ha сортата Куртовска капија е застапена на 3.000-3.500 ha главно во југоистичниот дел на Републиката. Ова сорта по своите генетски особини има високи вредности. Мегутоа, последните 8-10 години настана дегенерација на сортата при што приносот опадна за 30-35% од нејзиниот просечен потенцијал. Целта на испитувањата е подобрување на сортните особини со примена на одабирање и анализа на почетниот материјал (линии) за одбираwе на чист семенски материјал. Во 1998 година одбрани се вкупно 264 линии од 10 локалитети во Струмичко-радовишкиот реон од чисти посеви на оваа сорта. Со биометриските анализи утврдена е големина на плодовите, така да коефициентот на варирање (CV) кај поедини линии изнесува 5,2-21,5. Следната година (1999) издвоени се само 100 линии со понисок степен на варирање и засеани се 150-200 растенија од секоја линија. Деталните анализи во текот на вегетацијата и на основа биометријските анализи на плодовите како плус варијанти издвоени се 30 за понатамошно размножуваwе. Стастистичката анализа на поедини параметри на плодовите: 5 линии покажаа значајност, 4 доста значајни и 2 високо значајни позитивни разлики. Линиите се стабилни, со низок коефициент на варирање кои ги исполнува својствата на сортата. Избрани се 11 линии кои ќе бидат предмет на понатамошна работа со постојните методи да се постигне размножување на висока категорија на семенски материјал

Structural, biological and computational study of oxamide derivative|СТРУКТУРНА, БИОЛОШКА И РАЧУНСКА ИПИТИВАЊА ДЕРИВАТА ОКСАМИДА

A dicarboxylato-diamide-type compound 2,2'-[(1,2-dioxoethane-1,2--diyl)diimino]dibenzoicacid (H(4)obbz) (1) was synthesized and characterized. The crystal structure of K(2)H(2)obbz center dot 2H(2)O (2) was determined by X-ray diffract-tion analysis. The cytotoxic activities of the compounds were tested against four different cancer cell lines MCF-7, A549, HT-29, HeLa and a human nor-mal cell line MRC-5. The results indicate reasonable dose-dependent cytotox-icity of the ligands that show selectivity against the tested carcinoma and healthy cell lines. Flow cytometric analysis and fluorescence microscopy showed that the most active compound, H(4)obbz, induced apoptosis and G0/G1 cell cycle arrest, indicating blockage of DNA synthesis as a possible mechanism that trig-gers apoptosis. Docking and molecular dynamics simulations gave similar res-ponses regarding interactions (binding) between their ligands and chaperon Grp78. The MMGBSA determined Delta G binding energies were in the range from -104 to -140 kJ mol(-1)

Structural, biological and computational study of oxamide derivative

A dicarboxylato-diamide-type compound 2,2'-[(1,2-dioxoethane-1,2-diyl)diimino]dibenzoic acid (H4obbz) (1) was synthesized and characterized. The crystal structure of K2H2obbz·2H2O (2) was determined by X-ray diffracttion analysis. The cytotoxic activities of the compounds were tested against four different cancer cell lines MCF-7, A549, HT-29, HeLa and a human normal cell line MRC-5. The results indicate reasonable dose-dependent cytotoxicity of the ligands that show selectivity against the tested carcinoma and healthy cell lines. Flow cytometric analysis and fluorescence microscopy showed that the most active compound, H4obbz, induced apoptosis and G0/G1 cell cycle arrest, indicating blockage of DNA synthesis as a possible mechanism that triggers apoptosis. Docking and molecular dynamics simulations gave similar responses regarding interactions (binding) between their ligands and chaperon Grp78. The MMGBSA determined ΔG binding energies were in the range from -104 to -140 kJ mol-1,

Synthesis, structural analysis, solution equilibria and biological activity of rhodium(III) complexes with a quinquedentate polyaminopolycarboxylate

© 2017 The Royal Society of Chemistry. Two rhodium(iii) complexes [Rh(ed3a)(OH2)]·H2O (1) and Na[Rh(ed3a)Cl]·H2O (2) with ethylenediamine-N,N,N′-triacetate (ed3a) have been synthesized and characterized by elemental, spectroscopic and structural analyses. The crystal structure of (1) and (2) and the spectroscopic analysis of the two rhodium(iii)-ed3a complexes are discussed in detail. The protonation constants of H3ed3a and the conditional stability constants of its RhIII complexes have been determined in aqueous solution by pH potentiometry and UV-Vis spectrophotometry. Molecular mechanics (MM) and density functional theory (DFT) have been used to model all possible geometric isomers, determine the global energy minimum and compare the computed with the experimentally observed structures. The cytotoxic activity of the new RhIII complexes was evaluated by an MTT assay against four human cancer lines (MCF-7, A549, HT-29 and HeLa) and a normal human cell line (MRC-5). A549, HT-29 and HeLa cells were sensitive to all compounds tested, while the breast carcinoma cell line MCF-7 was only sensitive to the reference compounds (doxorubicin and cisplatin). Western blot (WB) analysis of the effects of the tested compounds indicates that both complexes increase the expression of caspase 3 and consequently the involvement of this enzyme in apoptotic processes of the treated cells. WB also demonstrates proteolytic cleavage of poly-(ADP-ribose)polymerase (PARP) in HeLa cells after treatment with both tested substances. Flow cytometry confirmed apoptotic cell death and showed the induction of cell cycle termination as a possible promoter of apoptosis

Synthesis, Cytotoxic Activity, and Thermal Studies of Novel N-[(1,3-Diphenylpyrazol-4-yl)methyl] alpha-Amino Acids

New N-[(1,3-diphenylpyrazol-4-yl)methyl]alpha-amino acids (1a-i) have been synthesized and tested in vitro for their antiproliferative activity against human myelogenous leukemia K562, colon adenocarcinoma HT-29, cervix carcinoma HeLa, and normal fetal lung fibroblasts, MRC-5. Compounds derived from both phenylalanine enantiomer precursors appeared to be the most active against myelogenous leukemia K562 cell lines with a high cytotoxic potential