Validation of a noninvasive aMMP-8 point-of-care diagnostic methodology in COVID-19 patients with periodontal disease

Objectives: The aim of this study was to validate an active matrix metalloproteinase (MMP-8) point-of-care diagnostic tool in COVID-19 patients with periodontal disease. Subjects, Materials, and Methods: Seventy-two COVID-19-positive and 30 COVID-19-negative subjects were enrolled in the study. Demographic data were recorded, periodontal examination carried out, and chairside tests run for evaluating the expression of active MMP-8 (aMMP-8) in the site with maximum periodontal breakdown via gingival crevicular fluid sampling as well as via a mouth rinse-based kit for general disease activity. In COVID-19-positive patients, the kits were run again once the patients turned COVID-19 negative. Results: The overall (n = 102) sensitivity/specificity of the mouthrinse-based kits to detect periodontal disease was 79.41%136.76% and that of site-specific kits was 64.71%/55.88% while adjusting for age, gender, and smoking status increased the sensitivity and specificity (82.35%/76.47% and 73.53%/88.24, respectively). Receiver operating characteristic (ROC) analysis for the adjusted model revealed very good area under the ROC curve 0.746-0.869 (p < .001) and 0.740-0.872 (p < .001) (the aMMP-8 mouth rinse and site-specific kits, respectively). No statistically significant difference was observed in the distribution of results of aMMP-8 mouth rinse test (p = .302) and aMMP-8 site-specific test (p = .189) once the subjects recovered from COVID-19. Conclusions: The findings of the present study support the aMMP-8 point-of-care testing (PoCT) kits as screening tools for periodontitis in COVID-19 patients. The overall screening accuracy can be further increased by utilizing adjunctively risk factors of periodontitis. The reported noninvasive, user-friendly, and objective PoCT diagnostic methodology may provide a way of stratifying risk groups, deciding upon referrals, and in the institution of diligent oral hygiene regimens.Peer reviewe

Karakterizacija solvatomorfa metotreksata pomoću termoanalitičkih i drugih metoda

Identification and characterization of different forms of methotrexate were carried out by crystallization from different solvents. Five different forms of the drug were obtained. Appearance of a desolvation endotherm in the DSC accompanied by mass loss in TGA for forms I, II, IV and V showed these forms to be acetonitrile solvate hydrate (form I), trihydrate (forms II and IV) and dimethylformamide solvate (form V), respectively. However, the desolvation peak was absent in form III (obtained from methanol) indicating the absence of any solvent of crystallization. This form was found to be partially crystalline by its XRPD pattern. Solution calorimetry was further used to differentiate between the forms as they differ in lattice energy, resulting in different enthalpies of solution. The dissolution and solubility profiles were correlated with the enthalpy of solution and subsequently with crystallinity of all the forms; the least endothermic form (form III) had the highest dissolution rate.U radu je provedena identifikacija i karakterizacija pet različitih formi metotreksata dobivenih kristalizacijom iz različitih otapala. Desolvatacijska izoterma u DSC popraćena gubitkom mase u TGA za forme I, II, IV i V ukazuje da su te forme solvati s acetonitrilom: hidrat (forma I), trihidrat (forma II i IV) i solvat s dimetilformamidom (forma V). Međutim, desolvatacijski pik je odsutan u formi III (dobivenoj iz metanola) što ukazuje na odsutnost otapala u kristalnoj formi. Ta forma je parcijalno kristalna i pokazuje odgovarajući XRPD uzorak. Energija kristalne ćelije je za različite forme različita, što ima za posljedicu različite entalpije otapanja te omogućava primjenu kalorimetrije otopine za diferencijaciju formi. Topljivost je korelirana s entalpijom otopine i kristaliničnosti svih formi. Najmanje endotermna forma (forma III) je najbolje topljiva

Discutindo a educação ambiental no cotidiano escolar: desenvolvimento de projetos na escola formação inicial e continuada de professores

A presente pesquisa buscou discutir como a Educação Ambiental (EA) vem sendo trabalhada, no Ensino Fundamental e como os docentes desta escola compreendem e vem inserindo a EA no cotidiano escolar., em uma escola estadual do município de Tangará da Serra/MT, Brasil. Para tanto, realizou-se entrevistas com os professores que fazem parte de um projeto interdisciplinar de EA na escola pesquisada. Verificou-se que o projeto da escola não vem conseguindo alcançar os objetivos propostos por: desconhecimento do mesmo, pelos professores; formação deficiente dos professores, não entendimento da EA como processo de ensino-aprendizagem, falta de recursos didáticos, planejamento inadequado das atividades. A partir dessa constatação, procurou-se debater a impossibilidade de tratar do tema fora do trabalho interdisciplinar, bem como, e principalmente, a importância de um estudo mais aprofundado de EA, vinculando teoria e prática, tanto na formação docente, como em projetos escolares, a fim de fugir do tradicional vínculo “EA e ecologia, lixo e horta”.Facultad de Humanidades y Ciencias de la Educació

Incompatibility Systems in Fruit Crops: Applications and Achievements

The method of pollination is crucial for fruit crop breeding since it affects the genetic makeup, type of gene action, ease of pollination control, and stability of varieties after release. There are a number of processes that encourage cross-pollination, but self-incompatibility (SI) is particularly significant since it is exploited in the production of hybrid seeds. SI is the practice of not allowing fertile (functioning) male and female gametes from the same plant to fuse together. Since ancient times, breeders and growers have successfully used SI as a tool to modify domesticated crops. Self-incompatibility may have an array of causes, including those that are physical, physiological, biochemical, and molecular, but on a broader scale, it appears that all of these components interact to regulate it. According to molecular investigations, at least two genes in the S-locus regulate the SI, one of which functions as a male and one as a female determinant. Self-incompatibility has several different uses, including marker-assisted breeding through SI genotyping, agricultural production and quality improvement, and the creation of hybrids to get over intra- and interspecific reproductive obstacles

Biophysical Characterization and Drug Delivery Potential of Exosomes from Human Wharton's Jelly-Derived Mesenchymal Stem Cells

Cell-derived exosomes (30-200 nm) as biological "nanocarriers" have attracted a great deal of interest for therapeutic applications due to their ability to internalize in in vivo biological systems (i.e., cells). Although they can be harvested from various sources including stem cells, yet an appropriate isolation and characterization protocol to obtain "pure" exosomal population is needed. For potential clinical applications, understanding the functional ability of exosomes and their purity, that is, free from microvesicles, apoptotic bodies, and protein aggregates, is a pre-requisite. To achieve high purity and yield of exosomes from human Wharton's jelly-derived mesenchymal stem cells (hWJ-MSCs) in the size range of 30-200 nm, we have performed and compared three isolation procedures: ultracentrifugation (UC), sucrose cushion (SC), and commercially available reagent (CR). The isolated exosomes were characterized using nanoparticle tracking analysis (NTA), field emission scanning electron microscopy (FESEM), and atomic force microscopy (AFM). Furthermore, to understand the therapeutic potential of the hWJ-MSC-derived exosomes (hWJ-ME) to target pancreatic tumor cells, the internalization efficacy has been evaluated on the MiaPaCa-2 cell lines using confocal microscopy and flow cytometry. The NTA results showed sucrose cushion to be an optimal method for exosome isolation with high purity (86.8%), as compared to UC (40.5%; p = 0.050) and CR (38%; p = 0.050). Optical analysis by FESEM and AFM revealed that SC-isolated exosomes presented a spherical morphology, whereas UC- and CR-isolated exosomes exhibited an uneven morphology. Furthermore, the data from confocal images and flow cytometry showed that hWJ-ME were internalized by MiaPaCa-2, demonstrating the feasibility of exosomes as a "potential nanocarrier". Thus, our study suggests that a combination of NTA (yield), AFM (dimensions), and FESEM (morphology and topography) could provide sensitive biophysical characterization of hWJ-ME. In the future, enriched exosomes could be used as a delivery vehicle to transport target-specific drugs or gene-silencing constructs to tumors

Vertical Farming: An Option in Modern Food Production: A Review

Meeting the food needs will be one of the major problems, as farmland is being lost due to causes including soil contamination, water scarcity, and climate change, among others. In this situation, a workable alternative to manage this perennial problem is provided by vertical farming, an energy-efficient, environmentally friendly agricultural technology that does not use soil. Vertical farming could indeed be an important factor in the production of crops and vegetables in regions with scarce soil and water resources. With modern technology such as hydroponics, aeroponics, and aquaponics, the idea of a vertical farm appears to have a promising future. In times of pandemics like COVID-19, it has emerged as a viable option for growing a wide range of food crops to suit the dietary needs of the growing world population

An Overview of Different Fruit Crop Models in the last 40 Years to Date with Their Main Uses

Horticulture is a versatile field which encompasses a plethora of day to day strategic decisions like varietal selection, optimisation of resources, understanding the mechanisms of the phenology, identification of plant invaders both in the micro and macro level, wise and judicious use of plant protectants, yield prediction &amp; assessment, post harvesting &amp; handling, strategic way of understanding the pulse of consumer’s popular demands and efficient way of marketing. Fruit trees are perennial unlike annual vegetable and cereal crops where there is a high prerequisite for efficient modelling of canopy architecture, photosynthesis, nutrient uptake, pest forecasting etc where the ill-effects of climate change are bringing out huge losses in the existing germplasm, annual turnover of the farmers and emergence of unheard pests and diseases. An invincible foresight or preparedness against such vagaries can be brought out by efficient modelling mechanisms combining the physiology, phenology and vital requirements of fruit trees with the interacting ecosystem of the land where it is present. Extrapolating such models from the local level to a general situations always gives fruitful results and it further aids in strengthening the present protocols. With the advancement of machine learning and deep learning in precision agriculture, problems of farmers and orchardists are being solved at a faster pace with the help of sensors in identification of problems and its alleviation using fast and error-free processing at pre-harvesting, harvesting and post-harvesting stages of fruit crops. In fact it is also one of the major concerns among people regarding the complete replacement of human power in the crucial decision support systems for agriculture and farming