Dietary Exposure Among Adults in Selangor, Malaysia, to Heterocyclic Amines and Polycyclic Aromatic Hydrocarbons in Cooked Meat and Fish

Heterocyclic amines (HCAs) and polycyclic aromatic hydrocarbons (PAHs) are possible human carcinogens and potent mutagens which increase the incidence of colon, mammary, prostate, breast and other cancers in rodents. Food containing meat and fish are the most important source of exposure to HCAs and PAHs in the diet and heat-treated foods, especially those which are fried, broiled and grilled. The intake of HCAs and PAHs are influenced by the amount and type of meat and fish ingested, frequency of consumption, cooking methods, cooking temperature and duration of cooking. The aim of the present study was to determine the level of HCAs and PAHs in the most consumed foods in Selangor, Malaysia in order to estimate their exposure to these toxic compounds. The dietary intake of HCAs and PAHs in foods consumed by people in Selangor, Malaysia was determined. Levels of six HCAs, namely: 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), 2-amino-3-4-dimethylimidazo[4,5-f] quinoline (MeIQ), 2-amino-3-8-dimethylimidazo[4,5-f]quinoxaline (MeIQx), 2-amino-3,4,8-trimethylimidazo[4,5-f]quinoxaline (4, 8-DiMeIQx), 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) and 2-amino-3,7,8-trimethylimidazo[4,5f] quinoxaline (7,8-DiMeIQx) and three PAHs, fluoranthene, benzo[b]fluoranthene and benzo[a]pyrene were measured. Forty-two samples of meat and fish were included in the study. High-performance liquid chromatography with photodiode array detector and fluorescence detector was used to analyze HCAs and PAHs, respectively. Dietary food consumption data (g/day), including meat type and cooking method were obtained using food frequency questionnaires, which were completed by 600 randomly selected subjects aged above 18 years. Results of the study showed that the level of total HCAs in food samples studied ranged from 0 to 38.7 ng/g whereas the level of total PAHs was, 0 to 66.28 ng/g. Among the analyzed HCAs and PAHs, PhIP (30.6 ng/g) and fluoranthene (50.96 ng/g) showed the highest level, respectively. The highest level of total HCAs was found in grilled chicken satay (38.7 ng/g) and for PAHs it was in grilled beef satay (66.28 ng/g). The most abundant HCAs such as PhIP and MeIQx, and for PAHs such as fluoranthene were detected in the food products studied. The 4,8-DiMeIQx, 7,8-DiMeIQx (HCAs) for HCAs and benzo[a]pyrene for PAHs were found in 12 and 22% of the meat and fish dishes. The average daily intake level of HCAs was 553.7 ng/capita/day and for PAHs of 297.58 ng/capita/day. The intake of PhIP was the highest, followed by MeIQx and MeIQ, whereas intake of fluoranthene was the highest, followed by benzo[b]fluoranthene and benzo[a]pyrene. The results reveal that grilled and fried meat and fish products were the major contributors to the exposure of HCAs and PAH

Dietary exposure to heterocyclic amines in high-temperature cooked meat and fish in Malaysia

The intake of heterocyclic amines is influenced by the amount and type of meat and fish ingested, frequency of consumption, cooking methods, cooking temperature, and duration of cooking. In this study, the dietary intake of heterocyclic amines in Malaysia and their main sources were investigated. Forty-two samples of meat and fish were analysed by high-performance liquid chromatography with photodiode array detector to determine the concentration of the six predominant heterocyclic amines, namely: 2-amino-3-methylimidazo[4,5-f] quinoline (IQ), 2-amino-3,4-dimethylimidazo[4,5-f] quinoline(MeIQ), 2-amino-3,8-dimethylimidazo[4,5-f] quinoxaline (MeIQx), 2-amino-3,4,8-trimethylimidazo[4,5-f] quinoxaline (4,8-DiMeIQx), 2-amino-3,7,8-trimethylimidazo[4,5-f] quinoxaline (7,8-DiMeIQx), and 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP). Dietary intake data were obtained using a food-frequency questionnaire when interviewing 600 Malaysian respondents. The level of total heterocyclic amines in food samples studies ranged from not detected to 38.7 ng g(-1). The average daily intake level of heterocyclic amine was 553.7 ng per capita day(-1). The intake of PhIP was the highest, followed by MeIQx and MeIQ. The results reveal that fried and grilled chicken were the major dietary source of heterocyclic amines in Malaysia. However, the heterocyclic amine intake by the Malaysian population was lower than those reported from other regions

Recent Advances in Disease Control by Natural Products in Animals and Birds in Bangladesh

Use of herbs for curing diseases is well documented in the history of all civilizations. Because of the increasing reports of the possible hazards in using synthetic products in animals, natural products are rapidly establishing their credibility. At least 60 different varieties of plants, herbs and shrubs grown in Bangladesh have recognized medicinal properties, and are being used by Village Doctors like Hekims and Quacks for treating diseases in man and animals. A number of natural products are commercially available in Bangladesh most of which are used as feed additives, though few have antibacterial and anticoccidial use. Recent studies revealed that ethanol extract of Ata (Annona reticulatd) at 2% concentration as spray showed highest efficacy (100%) followed by aqueous extract of Bishkatali (Polygonum hydropiper) against Boophilus microplus. Ethanol extracts of Neem (Azadirachta indica), bitter gourd (Momordica charantia) and Padmagulancha (Tinospora tomentosa) were highly effective against common stomach worm Haemonchus contortus in both in vitro and in vivo studies. Bitter gourd (Momordica charantia) has been found very effective against chicken coccidiosis. Birds receiving bitter gourd powder with feed had almost a similar weight gain compared with uninfected control chicks, and both these groups of chicks gained significantly higher (P<0.05) weight compared with chicks receiving sulphaclozine sodium. Anticipating the bright prospect, our research is now targeted mostly on the use of different plants against different parasitic infections in animals and birds. This presentation will cover the details of the currently used natural products in Bangladesh and our efforts in revealing the greatness of these natural products

Extraction of fish oil from the skin of Indian mackerel using supercritical fluids.

The total oil was extracted from the ground skin of Indian mackerel (Rastrelliger kanagurta) using various techniques of supercritical fluid extraction (SFE) at 20–35 MPa and 45–75 °C and by the Soxhlet method for comparison. The oil yield increased with pressure and temperature and the highest yields were 24.7, 53.2, 52.8, and 52.3/100 g sample (dry basis) for the continuous, cosolvent, soaking, and pressure swing techniques, respectively, at 35 MPa and 75 °C. The yield from the Soxhlet extraction was 53.6/100 g sample (dry basis). The CO2 consumption was 581.8, 493.6, 484.9 and 290.9 g for the continuous, cosolvent, soaking and pressure swing techniques, respectively, at 35 MPa and 75 °C. The largest recoveries of PUFA, especially the ω-3 family, were achieved from the soaking and pressure swing techniques at 35 MPa and 75 °C. Thus, the pressure swing and soaking techniques are the most effective at extracting the oil from fish skin

Cocoa butter fats and possibilities of substitution in food products concerning cocoa varieties, alternative sources, extraction methods, composition, and characteristics

The current concern for cocoa butter fat as major ingredients of chocolate intake in the World has raised the question of the high price of cocoa butter among all other vegetable fats. Productions of natural cocoa butter fats are decreasing day by day due to the decrease of cocoa cultivation worldwide; moreover, cocoa fruit contains only a little amount of cocoa butter. Therefore, the food industries are keen to find the alternatives to cocoa butter fat and this issue has been contemplated among food manufacturers. This review offers an update of scientific research conducted in relation to the alternative fats of cocoa butter from natural sources. The findings highlights how these cocoa butter alternatives are being produced either by blending, modifying the natural oils or fats from palm oil, palm kernel oil, mango seed kernel fats, kokum butter fat, sal fat, shea butter, and illipé fat

Supercritical Carbon Dioxide Extraction Of Mango Seed Kernel Fat Blended With Palm Oil Mid-Fraction And Palm Stearin To Formulate Cocoa Butter Replacers

Mango (Mangifera indica L.) is an important annual tropical fruit. Mango seed kernel (MSK) which is industrial by-product contains considerable amount of cocoa butter analogy fats. It is remarkable that no reports on the extraction of the mango seed kernel fat (MSKF) using supercritical carbon dioxide (SC-CO2) have been published. The aim of this work is to blend SC-CO2 extracted MSKF with palm oil mid-fraction (POMF) and palm stearin (PS) to formulate new cocoa butter replacers (CBRs). The physico-chemical properties, thermal properties, solid fat content (SFC) and morphology for the blends of MSKF: POMF and MSKF: PS conducted using different chromatographic and thermal techniques. Optimization of the SC-CO2 extraction parameters of MSKF from MSK were conducted using central composite design (CCD) of response surface methodology (RSM). The variables considered in the study are pressure (20-50 MPa), temperature (40-80 °C), and CO2 flow rate (1-4 ml/min). The optimized fat yield was predicted to be 11.29% at 44.2 MPa, 72.2 ºC and CO2 flow rate of 3.4 ml/min which was close to the fat yield (11.7%) of Soxhlet extraction. The blends containing 70 to 85% of MSKF had physico-chemical properties like fatty acid profiles, iodine value (IV), slip melting point (SMP), saponification value (SPV) and acid value (AV) close to that of commercial CB. Results showed that the major TG ranges in all blends were POP 11-38.8%, SOS 22.1-36.9%, and POS 15.4-16.2%, respectively

Open-vocabulary keyword spotting in any language through multilingual contrastive speech-phoneme pretraining

In this paper, we introduce a massively multilingual speech corpora with fine-grained phonemic transcriptions, encompassing more than 115 languages from diverse language families. Based on this multilingual dataset, we propose CLAP-IPA, a multilingual phoneme-speech contrastive embedding model capable of open-vocabulary matching between speech signals and phonemically transcribed keywords or arbitrary phrases. The proposed model has been tested on two fieldwork speech corpora in 97 unseen languages, exhibiting strong generalizability across languages. Comparison with a text-based model shows that using phonemes as modeling units enables much better crosslinguistic generalization than orthographic texts.Comment: Preprint; Work in Progres

IMPACT OF ASA MICRO-CREDIT PROGRAM TOW ARDS SOCIO­ ECONOMIC DEVELOPMENT OF RURAL WOMEN IN MONOHARDI UPAZILA OF NARSINGDI DISTRICT

A Thesis Submitted to the Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka, in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE IN AGRICULTURAL EXTENSION AND INFORMATION SYSTEMThe main focus of this study was to determine and describe the impact of ASA micro credit program on socio-economic development of rural women and to explore the relationship between the selected characteristics and impact of micro credit of ASA. Socio-economic development was measured by computing the different dimensions of change, such as change in income, change in food consumption, change in standard of living and change in decision-making ability of the credit receivers after their involvement in ASA micro credit program. The average annual income, per capita daily calorie intake, standard of living and decision making ability score of the respondents increased at 4.62 thousand taka, 144.87 kilo calories, 0.94 and I .50 respectively after involvement with ASA micro credit program. Before involvement, 67 per cent of the respondents had mud-builz houses with tin roof, after involvement the percentage was 74. Similarly 33 per cent of the respondents used half sanitary toilet before involvement where after involvement this percentage turned into 51 per cent. Before involvement with ASA micro-credit program, 19 per cent of the respondents' families had own tube well but after program this percentage was 38. The education, farm size, annual savings, cosmopoliteness, credit received, duration of involvement with ASA micro credit program and attitude towards ASA micro credit program had significant relationship with the impact of micro-credit. However, age and family size of the respondents did not show any significant relationship with the impact of ASA micro credit. The inadequate supply of credit facilities against their demand was identified as respondents' main problem and high rate of interest was the prime problem in case of repayment of loan. Based on the finding, it was concluded that micro-credit programme of ASA showed great potential for socio­ economic development on its beneficiaries. Therefore, it may be expanded all over Bangladesh for socio-economic development as well as poverty alleviation of the rural poor women

Value Chain Analysis of Brinjal in the Chittagong Hill Tracts of Bangladesh

This study examined the value chain and marketing margin of brinjal in Bandarban, Khagrachari and Chittagong of Bangladesh. It looks at profitability, value addition at different levels and marketing efficiency for the various market. The simple random sampling technique was followed for collecting primary data from the vegetable growers in the study area. In this study, total 60 growers and 50 market intermediaries were selected. A primary survey was carried out in January to June 2016. The benefit-Cost Ratio was used for estimating profitability. Marketing cost and margin were used for calculating the value addition in every stage of the supply chain. For assessing marketing efficiency, price spread, producers share, and Acharya’s methods were employed. The result revealed that brinjal cultivation was profitable since the Benefit-Cost Ratio (BCR) of brinjal cultivation was 1.59. The highest net marketing margin for brinjal was found in chain II. The most efficient marketing chain was found in chain III, which is Farmer → Retailer → Consumer (Local) in the study area. It appears that, based on the findings of the study, there is considerable scope exists for developing the value chain through keeping the marketing efficiency at the chain III level