Evaluation of anticonvulsant activity of angiotensin receptor antagonists in an animal model

Background: Epilepsy is common chronic disorder in clinical practice and there was some studies which shows brain renin angiotensin system may be involved in upregulation of seizures hence present study was planned to investigate whether angiotensin II AT1 receptor antagonists possess anticonvulsant activity in experimental animals.Methods: The anticonvulsant activity of angiotensin receptor antagonists, losartan (50 mg/kg), telmisartan (30 mg/kg) and candesartan (20 mg/kg), were administered intraperitoneally to the mice and evaluated by using maximum electroshock (MES) and pentylenetetrazol induced seizures (PTZ) seizure methods. The standard was taken as phenytoin for MES and diazepam for PTZ method. Motor impairment of performance was assessed by the inverted screen test and spontaneous motor activity with digital actophotometer.Results: Losartan demonstrated the anticonvulsant efficacy in MES and PTZ models. Telmisartan and candesartan have anticonvulsant activity in MES induced seizures, but did not show protection against pentylenetetrazol induced seizures. Losartan at dose 50 mg/kg prolonged the mean latency to convulsion (p<0.01) and mean number of convulsions also significantly reduced (p<0.05) convulsions in the mice. Telmisartan and candesartan at dose 30 mg/kg and 20 mg/kg respectively showed significant prolongation in mean latency to convulsion (p<0.05). None of the test drugs i.e. angiotensin receptor antagonists showed significant motor impairment.Conclusions: Angiotensin receptor antagonists: losartan, telmisartan and candesartan had showed anticonvulsant activity in PTZ and MES seizure methods. The exact mechanism of action of their anticonvulsant action not precisely known and hence there is more studies need to test it in various other animal anticonvulsant models

Some Contribution of Statistical Techniques in Big Data: A Review

Big Data is a popular topic in research work. Everyone is talking about big data, and it is believed that science, business, industry, government, society etc. will undergo a through change with the impact of big data.Big data is used to refer to very huge data set having large, more complex, hidden pattern, structured and unstructured nature of data with the difficulties to collect, storage, analysing for process or result. So proper advanced techniques to use to gain knowledge about big data. In big data research big challenge is created in storage, process, search, sharing, transfer, analysis and visualizing. To deeply discuss on introduction of big data, issue, management and all used big data techniques. Also in this paper present a review of various advanced statistical techniques to handling the key application of big data have large data set. These advanced techniques handle the structure as well as unstructured big data in different area

Isolation, Screening and Characterization of Exopolysaccharide Producing Pseudomonas Sp. from The Soil Sample

&nbsp; Exopolysaccharides (EPS) are vital metabolites that specific environmental microorganisms produce and expel. The goal of the current investigation is to identify bacteria that produce exopolysaccharides in soil samples from the Buttenath Dam region, At. Chanai, Tq. Ambajogai, Dist. Beed situated in the Marathwada region of Maharashtra, India. Two methods, such as gravimetric analysis of exopolysaccharide dry weight and quantification as, instance, for total carbohydrate content using phenol sulphuric acid technique, were used to screen the EPS-producing capacities of the chosen isolates. Post preliminary screening by selecting thick, ropy-like colony formers on agar medium, the results showed that thirteen (13) distinct colonies were marked to manufacture EPS. Tentative identification of these isolates was done based on morphological and biochemical tests. Three (03) isolates, VJ001, VJ003 and VJ010, were identified through secondary screening as the most effective EPS producers (producing precipitates over 1.46 mg/L of total dry weight and 8). This was in comparison to other bacterial colonies that were also isolated. Out of these screened isolates, VJ003 was the isolate with the highest exopolysaccharide produced and this isolated bacterium was identified using 16S rRNA gene sequence and phylogeny study as Pseudomonas aeruginosa VJ003 with NCBI GeneBank accession no. OR196093. The Pseudomonas aeruginosa VJ003 strain can be used in biotechnological sectors because of the ability it has to create significant levels of exopolysaccharides, according to the results of the current study

Bioelectricity Production and Comparative Evaluation of Electrode Materials in Microbial Fuel Cells Using Indigenous Anode-Reducing Bacterial Community From Wastewater of Rice-Based Industries

Microbial fuel cells (MFCs) are the electrochemical systems that harness the electricity production capacity of certain microbes from the reduction of biodegradable compounds. The present study aimed to develop mediator-less MFC without using expensive proton exchange membrane. In the present study, a triplicate of dual-chamber, mediator-less MFCs was operated with two local rice based industrial wastewater to explore the potential of this wastewater as a fuel option in these electrochemical systems. 30 combinations of 6 electrodes viz. Carbon (14 cm × 1.5 cm), Zn (14.9 cm × 4.9 cm), Cu (14.9 cm × 4.9 cm), Sn (14.1cm × 4.5cm), Fe (14cm × 4cm) and Al (14cm × 4.5 cm) were evaluated for each of the wastewater samples. Zn-C as anode-cathode combination produced a maximum voltage that was 1.084±0.016V and 1.086±0.028 and current of 1.777±0.115mA and 1.503±0.120 for KRM and SSR, respectively. In the present study, thick biofilm has been observed growing in MFC anode. Total 14 bacterial isolates growing in anode were obtained from two of the wastewater. The dual chambered, membrane-less and mediator-less MFCs were employed successfully to improve the economic feasibility of these electrochemical systems to generate bioelectricity and wastewater treatment simultaneously.Keywords: Membrane-less, Microbial Fuel Cells, Biofilm, Wastewater, Electrogenic.Article History: Received June 25th 2016; Received in revised form Dec 15th 2016; Accepted January 5th 2017; Available onlineHow to Cite This Article: Reena, M. and Jadhav, S. K. (2017) Bioelectricity production and Comparative Evaluation of Electrode Materials in Microbial Fuel Cells using Indigenous Anode-reducing Bacterial Community from Wastewater of Rice-based Industries. International Journal of Renewable Energy Develeopment, 6(1), 83-92.http://dx.doi.org/10.14710/ijred.6.1.83-9

Optimization of Process Parameters for CNC Turning using Taguchi Methods for EN24 Alloy Steel with Coated/Uncoated Tool Inserts

Coated and uncoated tool inserts offers certain degrees of control on the desired rate of tool wear and surface roughness to an extent. This work pursues the quest for realizing the optimal values for the significant process parameters that bears an influence on the response parameters. Experiments were conducted on the samples of EN 24 alloy steel material with the help of PVD coated TiAlN insert and uncoated carbide insert. The experimental runs carried out with proper variation in the levels. Levels are selected with the help of manufacturing catalogue and by pilot experimentation and results are recorded for further analysis. For this study, 9 runs designed using L9 orthogonal array of Taguchi Design of Experiment. Surface roughness was measured using a Mitutoyo surface tester at test lab and material removal rate is calculated by mathematical equation. The data was compiled into Minitab 17 software for analysis. The relationship between the machining parameters and the response variables were analyzed using the Taguchi Method. Optimization of process parameters is carried out by Grey Relational Analysis method (GRA). GRA method is a powerful and most versatile tool which can manipulate the input data as per requirement and comes with results that can be used to have best multi-objective in respective concerns

Documentation and ethnobotanical survey of wild edible plants from Kolhapur district

The present study deals with the identification, documentation and ethno-botanical exploration with respect to food value of wild edible plants from Kolhapur district. Total 50 wild edible plants were surveyed. Edible parts of wild plants (fruit, flower, leaves, tubers and inflorescences) are the nature’s gift to mankind; these are not only delicious and refreshing but also the chief source of vitamin, minerals and protein. The wild edible plants are the normal food of cattle grazers and the forest tribes. Although the popularity of these wild forms of fruits, flowers and tubers has declined, it is considered that special attention should be paid to them in order to maintain and improve this important source of food supply

Drug utilization pattern in geriatric out patient in tertiary care hospital

Background: Inappropriate drug prescribing is a global problem affecting the healthcare system. Aim and objective of the study was to study the drug utilization pattern in geriatric patient at rural tertiary care hospital.Methods: This was a cross- sectional observational study involving 600 geriatric outpatient. This study was carried out from Nov 2015 to May 2016. The data were collected using predesigned proforma specially designed for this purpose. Relevant information was obtained by analyzing prescription for World Health Organization (WHO) core drug indicators.Results: Total number 600 prescriptions analyzed at the end of six months were from general medicine department. The mean age of the patients was 63.9 years in which male (61.33%) outnumber the female. Total number 2598 drugs were prescribed to 600 patients for different diseases. The mean number of drugs per prescription were 4.33, drugs were prescribed by generic name 26.42%. drugs were prescribed from WHO essential drug list86.33. The type of formulations used were tablets and capsules in 88.5%, syrups in 5%, injections 3.5 % and inhalers 2%. Drug for Cardiovascular diseases were the most common (29.66) followed gastrointestinal (16.67), vitamins and minerals (14.66). Analgesic and NSAIDS (14.13) also prescribed commonly. Antimicrobial drugs prescribed in (7.46%) and common antibiotic prescribed were Amoxicillin ciprofloxacin and metronidazole.Conclusions: This study also effectively provides very useful baseline data also demonstrates the prescribing patterns of drugs in the geriatric patients

Packed hybrid silica nanoparticles as sorbents with thermo-switchable surface chemistry and pore size for fast extraction of environmental pollutants

Thermoresponsive poly(N-isopropylacrylamide)-grafted silica nanoparticles (SiNPs) have been synthesized and fully characterized by ATR-FTIR, TGA, HRTEM, BET and DLS analysis. Hybrid solid phase extraction (SPE) beds with tuneable pore size and switchable surface chemistry were prepared by packing the polymergrafted nanoparticles inside SPE cartridges. The cartridges were tested by checking the thermoregulated elution of model compounds, namely methylene blue, caffeine and amoxicillin. Extraction of the analytes and regeneration of the interaction sites on the sorbent surface was carried out entirely in water solution by changing the external temperature below and above the lower critical solution temperature (LCST) of the polymer. The results demonstrate that the elution of model compounds depends on the temperature-regulated size of the inter-particle voids and on the change of surface properties of the PNIPAM-grafted nanoparticles from hydrophilic to hydrophobic

Study of early perinatal outcome in lower segment caesarean section in severe foetal distress at tertiary care centre

Background: For asphyxia, the fetus reacts with a series of responses. First there is redistribution of blood flow to vital centres to limit the deleterious effects of oxygen limitation in the brain, heart and adrenal glands. A further compensatory response is that overall fetal oxygen consumption declines to values as low as 50% of the control.Methods: This was a prospective study. Early perinatal outcome of newborns delivered through Caesarean section due to clinical foetal distress in labour was compared with a group of newborns similarly delivered via Caesarean section without a diagnosis of clinical foetal distress. Data collected and analysed using appropriate standard statistical methods i.e. Chi-square (X2) test and ‘Z’ - test.Results: Most common indication for NICU admission in study group was MAS (Meconium Aspiration Syndrome 14%) followed by MAS with perinatal asphyxia (5.33%), MAS with Hypoxic ischaemic encephalopathy (HIE) - stage III (3.33%) paerintal asphyxia (2.66%), severe PNA with HIE - III (0.66%) and TTN (0.66%) meconium gastritis (0.66%) respectively.Conclusions: Clinical foetal distress (study group) was found to be significantly associated with low 1 min and 5 min. Apgar score. There was no significant difference in immediate NICU admission whether D-D (i.e. detection fetal detection to delivery interval) time interval 30 minutes. But rate of mortality was high when D-D (i.e. detection fetal detection to delivery interval) was >30 minutes