MOESM1 of Interference between surgical magnetic drapes and pacemakers: an observational study comparing commercially available devices and a new magnetically isolated drape

Additional file 1. Patent for the LT10GTM drape from Menodys: US patent 9,232,976. Dated January 12th, 2016. http://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO1&Sect2=HITOFF&d=PALL&p=1&u=%2Fnetahtml%2FPTO%2Fsrchnum.htm&r=1&f=G&l=50&s1=9232976.PN.&OS=PN/9232976&RS=PN/9232976

Temperature-dependence of Glu-AMS binding to GluRS.

<p>Integrated ITC curves of Glu-AMS (90 μM) binding to GluRS (9 μM) at different temperatures; 20°C (circles), 30°C (upside-down triangles), 37°C (squares).</p

Influence of tRNA on GluRS/Glu-AMS binding at 30°C.

<p>n = stoichiometry coefficient (number of moles of Glu-AMS bound per mole of GluRS monomer), ΔH_b = reaction enthalpy, ΔS_b = reaction entropy, ΔG_b = reaction energy (calculated with the formula ΔG_b = -RT Ln <i>K</i>_b, where R = 1.987 cal/mol·K).</p><p>All values and errors in this table were obtained by weighting by inverse variance [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0121043#pone.0121043.ref032" target="_blank">32</a>], except for ΔG_b values and errors, obtained by simple average and standard error calculations.</p><p>Raw data and calculated values for each separated ITC runs are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0121043#pone.0121043.s004" target="_blank">S1 Table</a>.</p><p>Influence of tRNA on GluRS/Glu-AMS binding at 30°C.</p

Temperature-dependance of the GluRS Glu-AMS interaction.

<p>n = stoichiometry coefficient (number of moles of Glu-AMS bound per mole of GluRS monomer), ΔH_b = reaction enthalpy, ΔS_b = reaction entropy, T = temperature, ΔG_b = reaction energy (calculated with the formula ΔG_b = -RT Ln <i>K</i>_b, where R = 1.987 cal/mol·K).</p><p>Injections of Glu-AMS at 90 μM were done in a starting concentration of 9 μM of GluRS.</p><p>Raw data and calculated values for each separated ITC runs are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0121043#pone.0121043.s005" target="_blank">S2 Table</a>.</p><p>Temperature-dependance of the GluRS Glu-AMS interaction.</p

Glu-AMS binding to GluRS with/without tRNA.

<p>(a) Integrated ITC curves of Glu-AMS binding to GluRS with/without tRNA. Binding of Glu-AMS: to GluRS alone (circles), to GluRS with saturating concentration of tRNA^Glu in enriched total tRNA from <i>E</i>. <i>coli</i> (upside-down triangles), to GluRS with 11.23 µM tRNA^Phe from brewer’s yeast (squares). A duplicata of each tested condition is shown. The values shown in (b) were calculated from means of two distinct experiments reported in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0121043#pone.0121043.t001" target="_blank">Table 1</a>, weighting by inverse variance [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0121043#pone.0121043.ref032" target="_blank">32</a>].</p

Assessments of the suitability of RNA and DNA extracted from prioritized kits for typical down-stream applications.

<p>(A) NanoString-based nCounter results showing counts of RNA extracted from four kits plus RNA extracted from fresh PC-3 cells (using the RNeasy kit). Signal counts from each of the 48 genes are shown as heatmaps (in log₂ scale), with red and green representing the highest and lowest number of counts, respectively. (B) Cycle threshold (Cq) values in duplicate from RT-qPCR of three housekeeping genes (<i>PGK1</i>, <i>KRT8</i>, and <i>HPRT1</i>) using RNA extracted via four kits. (C) Cq values from MS-PCR for three genes commonly methylated in prostate cancer (<i>RASSF1A</i>, <i>GSTP1</i>, and <i>ABCB1</i>) as well as <i>Alu</i> repeats (as a control) across the five prioritized DNA kits. Data points for replicates are shown, with lines indicating the median Cq values. See supplementary <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0179732#pone.0179732.s006" target="_blank">S2 Table</a> for more detailed data and statistical analysis.</p

Analysis of PSA recurrence-free survival.

a<p>Log rank test (univariate analysis) or Wald test (multivariate analysis).</p>b<p>Analyzed as a continuous variable.</p>c<p>Stratification based on limited representation of Gleason 6 and 4+4 cases.</p>d<p>Stratifies pathologic stage based on organ confinement.</p

Clinicopathological characteristics of tissue microarray cases.

a<p>Determined from pathology report (not tissue microarray core).</p

Inhibition assays.

<p>Inhibition assays were set up as qPCR reactions using murine genomic DNA; a primer set specific to the mouse-<i>HSD11β1</i> gene (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0179732#pone.0179732.s005" target="_blank">S1 Table</a>); and the PowerUp SYBR Green Master Mix. The reaction mixture was spiked with water (as a control) or extracted RNA or DNA from various kits. Shown are the Cq values for the control vs. reactions spiked with RNA (A) and DNA (B), in duplicate, with error bars representing standard deviations. (C) Fragment distribution of amplified RNA and DNA samples from select kits with corresponding positive controls (fresh PC-3 cells).</p

Incorporation of proliferation index improves prognostic value.

<p>(<b>A</b>) Kaplan-Meier analysis comparing multivariate models based on clinicopathologic data (<i>left</i>) and clinicopathologic data plus the tri-marker proliferation index (<i>right</i>). Cases are grouped by tertile. Log-rank test <i>P</i>-values are indicated. (<b>B</b>) ROC curve analysis comparing multivariate models based on clinicopathologic data (<i>left</i>) and clinicopathologic data plus the tri-marker proliferation index (<i>right</i>). Analysis done at 8 years follow-up (the median follow-up time for the cohort). Areas under the curve (AUC) are indicated.</p