Steam treatment of contaminated groundwater aquifers – development of pathogenic micro-organisms in soil

Steam treatment of contaminated soil and aquifer sediment is a promising method of cleaning soil. The treatment is based on steam injection into a water saturated porous aquifer (Gudbjerg et al. 2004), by which the heat transfers the contaminants into the vapour phase, allowing entrapment in an active carbon filter connected to a large vacuum suction device. The treatment is effective against several important groundwater contaminants, including pentachlorophenole and perchloroethylene, typically found in association with industrial processes or dry cleaning facilities. Furthermore, as an example of removal of non-aqueous phase liquids (NAPLs) large amounts of creosote have been recovered after steam injection in a deep aquifer (Kuhlmann 2002; Tse & Lo 2002). Steam treatment is dependent on the complete heating of the soil volume under treatment. The steam has a strongly adverse impact on trees and other plants with deep root systems within the soil, but no other visible effects have been reported. The aim of the activities undertaken during collaborative projects carried out by the Geological Survey of Denmark and Greenland (GEUS) and the Danish Institute of Agricultural Sciences (DJF) for the Danish Environmental Protection Agency and the local authorities in Copenhagen (Københavns Amt) was to establish to what extent the microbial community was affected by the steam treatment of the soil. A few results from the literature indicate that the microbial activity increases in steam treated soil (Richardson et al. 2002), probably due to microbial degradation of the soil contaminants in combination with microbial utilisation of heatkilled organisms. It is, however, not known whether this increased microbial activity is associated with the development of pathogenic micro-organisms; these are typically able to grow at higher temperatures than the general microbial community in soil

Permafrost meta-omics and climate change

Permanently frozen soil, or permafrost, covers a large portion of the Earth's terrestrial surface and represents a unique environment for cold-adapted microorganisms. As permafrost thaws, previously protected organic matter becomes available for microbial degradation. Microbes that decompose soil carbon produce carbon dioxide and other greenhouse gases, contributing substantially to climate change. Next-generation sequencing and other -omics technologies offer opportunities to discover the mechanisms by which microbial communities regulate the loss of carbon and the emission of greenhouse gases from thawing permafrost regions. Analysis of nucleic acids and proteins taken directly from permafrost-associated soils has provided new insights into microbial communities and their functions in Arctic environments that are increasingly impacted by climate change. In this article we review current information from various molecular -omics studies on permafrost microbial ecology and explore the relevance of these insights to our current understanding of the dynamics of permafrost loss due to climate change

Glacial microbiota are hydrologically connected and temporally variable

Glaciers are melting rapidly. The concurrent export of microbial assemblages alongside glacial meltwater is expected to impact the ecology of adjoining ecosystems. Currently, the source of exported assemblages is poorly understood, yet this information may be critical for understanding how current and future glacial melt seasons may influence downstream environments. We report on the connectivity and temporal variability of microbiota sampled from supraglacial, subglacial and periglacial habitats and water bodies within a glacial catchment. Sampled assemblages showed evidence of being biologically connected through hydrological flowpaths, leading to a meltwater system that accumulates prokaryotic biota as it travels downstream. Temporal changes in the connected assemblages were similarly observed. Snow assemblages changed markedly throughout the sample period, likely reflecting changes in the surrounding environment. Changes in supraglacial meltwater assemblages reflected the transition of the glacial surface from snow‐covered to bare‐ice. Marked snowmelt across the surrounding periglacial environment resulted in the flushing of soil assemblages into the riverine system. In contrast, surface ice within the ablation zone and subglacial meltwaters remained relatively stable throughout the sample period. Our results are indicative that changes in snow and ice melt across glacial environments will influence the abundance and diversity of microbial assemblages transported downstream