3 research outputs found
Roles of DNA polymerase I in leading and lagging-strand replication defined by a high-resolution mutation footprint of ColE1 plasmid replication
DNA polymerase I (pol I) processes RNA primers during lagging-strand synthesis and fills small gaps during DNA repair reactions. However, it is unclear how pol I and pol III work together during replication and repair or how extensive pol I processing of Okazaki fragments is in vivo. Here, we address these questions by analyzing pol I mutations generated through error-prone replication of ColE1 plasmids. The data were obtained by direct sequencing, allowing an accurate determination of the mutation spectrum and distribution. Pol I’s mutational footprint suggests: (i) during leading-strand replication pol I is gradually replaced by pol III over at least 1.3 kb; (ii) pol I processing of Okazaki fragments is limited to ∼20 nt and (iii) the size of Okazaki fragments is short (∼250 nt). While based on ColE1 plasmid replication, our findings are likely relevant to other pol I replicative processes such as chromosomal replication and DNA repair, which differ from ColE1 replication mostly at the recruitment steps. This mutation footprinting approach should help establish the role of other prokaryotic or eukaryotic polymerases in vivo, and provides a tool to investigate how sequence topology, DNA damage, or interactions with protein partners may affect the function of individual DNA polymerases
Thymosin β4 impeded murine stem cell proliferation with an intact cardiovascular differentiation
Loss of the Martian atmosphere to space: Present-day loss rates determined from MAVEN observations and integrated loss through time
International audienceObservations of the Mars upper atmosphere made from the Mars Atmosphere and Volatile Evolution (MAVEN) spacecraft have been used to determine the loss rates of gas from the upper atmosphere to space for a complete Mars year (16 Nov 2014 – 3 Oct 2016). Loss rates for H and O are sufficient to remove ∼2-3 kg/s to space. By itself, this loss would be significant over the history of the planet. In addition, loss rates would have been greater early in history due to the enhanced solar EUV and more-active Sun. Integrated loss, based on current processes whose escape rates in the past are adjusted according to expected solar evolution, would have been as much as 0.8 bar CO2 or 23 m global equivalent layer of H2O; these losses are likely to be lower limits due to the nature of the extrapolation of loss rates to the earliest times. Combined with the lack of surface or subsurface reservoirs for CO2 that could hold remnants of an early, thick atmosphere, these results suggest that loss of gas to space has been the dominant process responsible for changing the climate of Mars from an early, warmer environment to the cold, dry one that we see today