Multiple defect interpretation based on Gaussian processes for MFL technology

Magnetic Flux Leakage (MFL) technology has been used in non-destructive testing for more than three decades. There have been several publications in detecting and sizing defects on metal pipes using machine learning techniques. Most of these literature focus on isolated defects, which is far from the real scenario. This study is towards the generalization of interpretation of the leakage flux in the presence of multiple defects based on simulation models, together with data-driven inference methodologies, such as Gaussian Process (GP) models. A MFL device has been simulated using both COMSOL Multiphysics and ANSYS software followed by prototyping the same device for experimental validations. Multiple defects with different geometrical configurations were introduced on a cast iron pipe sample and both radial and axial components of the leakage field have been measured. It was observed that both axial and radial components differ with different defect configurations. We propose to use GP to solve the inverse model problem by capturing such behaviors, i.e. to recover the profille of a cluster of defects from the measurements of a MFL device. The data was used to learn the non-parametric GP model with squared exponential covariance function and automatic relevance determination to solve this regression problem. Extensive quantitative and qualitative evaluations are presented using simulated and experimental data that validate the success of the proposed non-parametric methodology for interpreting the profiling of clusters of defects with MFL technology. © 2013 SPIE

Constrained sampling of 2.5D probabilistic maps for augmented inference

© 2016 IEEE. This work exploits modeling spatial correlation in 2.5D data using Gaussian Processes (GPs), and produces constrained sampling realizations on these models to improve certainty in the predictions by means of integrating additional sparse information. Data organized in 2.5D such as elevation and thickness maps has been extensively studied in the fields of robotics and geostatistics. These maps are typically represented as a probabilistic 2D grid that stores an estimated value (height or thickness) for each cell. With the increasing popularity and deployment of robotic devices for infrastructure inspection, 2.5D data becomes a common interpretation of the condition of the target being inspected. Modeling the spatial dependencies and making inferences on new grid locations is a common task that has been addressed using GPs, but inference results on locations which are weakly correlated with the training data are generally not sufficiently informative and distinctly uncertain. The predictive capability of the proposed framework, which is applicable to any 2.5D data, is demonstrated with field inspection data from pipelines. Specifically, sparse and complementary measurements from alternative sensing modalities have been incorporated into the model to predict in more detail local thickness conditions where GP training data is limited. The output of this work aims to probabilistically present variations of the target in the case that both accuracy and reasonable diversity are of significant interest

Heterobiaryl synthesis by contractive C-C coupling via P(V) intermediates

Heterobiaryls composed of pyridine and diazine rings are key components of pharmaceuticals and are often central to pharmacological function. We present an alternative approach to metal-catalyzed cross-coupling to make heterobiaryls using contractive phosphorus C–C couplings, also termed phosphorus ligand coupling reactions. The process starts by regioselective phosphorus substitution of the C–H bonds para to nitrogen in two successive heterocycles; ligand coupling is then triggered via acidic alcohol solutions to form the heterobiaryl bond. Mechanistic studies imply that ligand coupling is an asynchronous process involving migration of one heterocycle to the ipso position of the other around a central pentacoordinate P(V) atom. The strategy can be applied to complex drug-like molecules containing multiple reactive sites and polar functional groups, and also enables convergent coupling of drug fragments and late-stage heteroarylation of pharmaceuticals

Voxel-wise comparisons of cellular microstructure and diffusion-MRI in mouse hippocampus using 3D Bridging of Optically-clear histology with Neuroimaging Data (3D-BOND)

A key challenge in medical imaging is determining a precise correspondence between image properties and tissue microstructure. This comparison is hindered by disparate scales and resolutions between medical imaging and histology. We present a new technique, 3D Bridging of Optically-clear histology with Neuroimaging Data (3D-BOND), for registering medical images with 3D histology to overcome these limitations. Ex vivo 120 × 120 × 200 μm resolution diffusion-MRI (dMRI) data was acquired at 7 T from adult C57Bl/6 mouse hippocampus. Tissue was then optically cleared using CLARITY and stained with cellular markers and confocal microscopy used to produce high-resolution images of the 3D-tissue microstructure. For each sample, a dense array of hippocampal landmarks was used to drive registration between upsampled dMRI data and the corresponding confocal images. The cell population in each MRI voxel was determined within hippocampal subregions and compared to MRI-derived metrics. 3D-BOND provided robust voxel-wise, cellular correlates of dMRI data. CA1 pyramidal and dentate gyrus granular layers had significantly different mean diffusivity (p > 0.001), which was related to microstructural features. Overall, mean and radial diffusivity correlated with cell and axon density and fractional anisotropy with astrocyte density, while apparent fibre density correlated negatively with axon density. Astrocytes, axons and blood vessels correlated to tensor orientation

Intermedin attenuates LPS-induced inflammation in the rat testis

First reported as a vasoactive peptide in the cardiovascular system, intermedin (IMD), also known as adrenomedullin 2 (ADM2), is a hormone with multiple potent roles, including its antioxidant action on the pulmonary, central nervous, cardiovascular and renal systems. Though IMD may play certain roles in trophoblast cell invasion, early embryonic development and cumulus cell-oocyte interaction, the role of IMD in the male reproductive system has yet to be investigated. This paper reports our findings on the gene expression of IMD, its receptor components and its protein localization in the testes. In a rat model, bacterial lippolysaccharide (LPS) induced atypical orchitis, and LPS treatment upregulated the expression of IMD and one of its receptor component proteins, i.e. receptor activity modifying protein 2 (RAMP2). IMD decreased both plasma and testicular levels of reactive oxygen species (ROS) production, attenuated the increase in the gene expression of the proinflammatory cytokines tumor necrosis factor alpha (TNFα), interleukin 6 (IL6) and interleukin 1 beta (IL1β), rescued spermatogenesis, and prevented the decrease in plasma testosterone levels caused by LPS. The restorative effect of IMD on steroidogenesis was also observed in hydrogen peroxide-treated rat primary Leydig cells culture. Our results indicate IMD plays an important protective role in spermatogenesis and steroidogenesis, suggesting therapeutic potential for IMD in pathological conditions such as orchitis.published_or_final_versio

Ultrasound hyperthermia induces apoptosis in head and neck squamous cell carcinoma : an in vitro study

Hyperthermia is considered an efficient complement in the treatment of head and neck squamous cell carcinoma (HNSCC). Hyperthermia induces cell apoptosis in a temperature- and time-dependent manner. However, the molecular mechanism of hyperthermia remains unclear. The aim of this study was to investigate the mechanism of apoptosis induced by ultrasound hyperthermia in HNSCC cell lines HN-30 and HN-13. We examined the dynamic changes of early apoptosis and secondary necrosis in HN-30 and HN-13 cells treated by hyperthermia at 42°C for 10 min. We further examined mitochondrial membrane potential in vitro by ultrasound hyperthermia for different heating temperatures (38-44°C, 10 min) and heating times (42°C, 10-50 min). After heating by ultrasound at 42°C for 10 min, the apoptosis index achieved its highest level at 8 h after treatment, decreased rapidly and remained constant at a reduced level at 12 h. The level of secondary necrosis increased with the level of early apoptosis but remained at a higher level until 14 h. The level of secondary necrosis correlated with the level of early apoptosis (HN-13: r=0.7523, P=0.0030; HN-30: r=0.6510, P=0.016). The fractions of cells with low mitochondrial membrane potential (??) in the heating-temperature grads group and heating-time grads group decreased significantly over time. Therefore, HN-30 and HN-13 cells developed apoptosis after ultrasound hyperthermia treatment with decreases in the mitochondrial transmembrane potential level. Ultrasound hyperthermia induces apoptosis in HN-30 and HN-13 cells, possibly via the mitochondrial caspase pathway

An exploration of the collaborative processes of making theatre inspired by science

This research examined the collaborative processes of making theatre inspired by science through the analysis of 16 semi-structured interviews with individual collaborators (eight theatre practitioners and eight scientists). Interviews explored experiences, including their motivations, working processes, challenges, learning and understanding. Roles of scientists in the collaboration ranged from expert advisor to equal creative collaborator. Factors affecting partnerships included curiosity for each other's practice, social interaction and mutual respect. The research suggests that scientists could be motivated to undertake 'Sci-Art' collaborations through personal interest, as well as previously identified motives such as encouragement from their department. The project also identified benefits to researchers from such collaborations, including developing new perspectives on their own practice. © The Author(s) 2011

Proteomic profile of KSR1-regulated signalling in response to genotoxic agents in breast cancer

Kinase suppressor of Ras 1 (KSR1) has been implicated in tumorigenesis in multiple cancers, including skin, pancreatic and lung carcinomas. However, our recent study revealed a role of KSR1 as a tumour suppressor in breast cancer, the expression of which is potentially correlated with chemotherapy response. Here, we aimed to further elucidate the KSR1-regulated signalling in response to genotoxic agents in breast cancer. Stable isotope labelling by amino acids in cell culture (SILAC) coupled to high-resolution mass spectrometry (MS) was implemented to globally characterise cellular protein levels induced by KSR1 in the presence of doxorubicin or etoposide. The acquired proteomic signature was compared and GO-STRING analysis was subsequently performed to illustrate the activated functional signalling networks. Furthermore, the clinical associations of KSR1 with identified targets and their relevance in chemotherapy response were examined in breast cancer patients. We reveal a comprehensive repertoire of thousands of proteins identified in each dataset and compare the unique proteomic profiles as well as functional connections modulated by KSR1 after doxorubicin (Doxo-KSR1) or etoposide (Etop-KSR1) stimulus. From the up-regulated top hits, several proteins, including STAT1, ISG15 and TAP1 are also found to be positively associated with KSR1 expression in patient samples. Moreover, high KSR1 expression, as well as high abundance of these proteins, is correlated with better survival in breast cancer patients who underwent chemotherapy. In aggregate, our data exemplify a broad functional network conferred by KSR1 with genotoxic agents and highlight its implication in predicting chemotherapy response in breast cancer