p63 expression in sarcomatoid/metaplastic carcinomas of the breast

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Multiple immunofluorescence labelling of formalin-fixed paraffin-embedded (FFPE) tissue

<p>Abstract</p> <p>Background</p> <p>Investigating the expression of candidate genes in tissue samples usually involves either immunohistochemical labelling of formalin-fixed paraffin-embedded (FFPE) sections or immunofluorescence labelling of cryosections. Although both of these methods provide essential data, both have important limitations as research tools. Consequently, there is a demand in the research community to be able to perform routine, high quality immunofluorescence labelling of FFPE tissues.</p> <p>Results</p> <p>We present here a robust optimised method for high resolution immunofluorescence labelling of FFPE tissues, which involves the combination of antigen retrieval, indirect immunofluorescence and confocal laser scanning microscopy. We demonstrate the utility of this method with examples of immunofluorescence labelling of human kidney, human breast and a tissue microarray of invasive human breast cancers. Finally, we demonstrate that stained slides can be stored in the short term at 4°C or in the longer term at -20°C prior to images being collected. This approach has the potential to unlock a large in vivo database for immunofluorescence investigations and has the major advantages over immunohistochemistry in that it provides higher resolution imaging of antigen localization and the ability to label multiple antigens simultaneously.</p> <p>Conclusion</p> <p>This method provides a link between the cell biology and pathology communities. For the cell biologist, it will enable them to utilise the vast archive of pathology specimens to advance their <it>in vitro </it>data into <it>in vivo </it>samples, in particular archival material and tissue microarrays. For the pathologist, it will enable them to utilise multiple antibodies on a single section to characterise particular cell populations or to test multiple biomarkers in limited samples and define with greater accuracy cellular heterogeneity in tissue samples.</p

Toxicological Study Employing Repeated Doses of Garcinielliptone FC, a Polyisoprenylated-Benzophenone Isolated from Seed of Platonia Insignis Mart

The major constituent from the hexane extract of the seeds of P. insignis is GFC (garcinielliptone FC). Doses of 25, 50and 75 mg/kg of GFC were aseptically suspended in 0.05% Tween 80 dissolved in 0.9% saline (vehicle) and orally administered for30, 90 and 120 consecutive days to adult Swiss mice. In this work, the repeated oral administration, in animals of both sexes,demonstrates that this compound is not able to induce mortality and/or behavioral changes in adult mice. In addition, body weightgain, feed intake and disposal of excreta were not altered by the administration of this compound with repeated doses. Furthermore,no differences in weight and macroscopic structure of the brain, liver, kidney, lung, heart and spleen between groups of male andfemale adult mice were observed after treatment. During the periods of treatment, GFC produced no significant changes onhaematological and biochemical parameters in male and female mice treated with all doses used. The aim of this study was toinvestigate the toxicological potential of GFC through behavioral, hematological, biochemical and morphological parameters inanimals in order to ensure the safe use of Platonia insignis in folk medicine.Fil: Silva, Ana P.. Federal University of Piauí; BrasilFil: Filho, José Carlos C. L. S.. North Union of Parana; BrasilFil: da Costa Júnior, Joaquim S.. Federal Institute of Piauí; BrasilFil: Peláez, Walter José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Faillace, Martín Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Falcão Ferraz, Alexandre de B.. Lutheran University of Brazil; BrasilFil: David, Jorge M.. Institute Of Chemistry, Federal University Of Bahia; Brasil. Universidade Federal da Bahia; BrasilFil: Freitas, Rivelilson M.. Federal University of Bahia; Brasi

Evaluation Of A Program To Reduce Back Pain In Nursing Personnel.

To evaluate the effectiveness of a program designed to reduce back pain in nursing aides. Female nursing aides from a university hospital who had suffered episodes of back pain for at least six months were included in the study. Participants were randomly divided into a control group and an intervention group. The intervention program involved a set of exercises and an educational component stressing the ergonomic aspect, administered twice a week during working hours for four months. All subjects answered a structured questionnaire and the intensity of pain was assessed before and after the program using a visual analogue scale (VAS). Student's t-test or the Wilcoxon Rank Sum Test for independent samples, and Chi-square test or the Exact Fisher test for categorical analysis, were used. The McNemar test and the Wilcoxon matched pairs test were used to compare the periods before and after the program. There was a statistically significant decrease in the frequency of cervical pain in the last two months and in the last seven days in the intervention group. There was also a reduction in cervical pain intensity in the two periods (2 months, 7 days) and lumbar pain intensity in the last 7 days. The results suggest that a program of regular exercise with an emphasis on ergonomics can reduce musculoskeletal symptoms in nursing personnel.35356-6

Segundo tempo-forças no esporte: o esporte de alto rendimento como legado dos LMMS

O esporte brasileiro vislumbrou, como meta, no decorrer das duas primeiras décadas do século XXI, o cumprimento de uma agenda esportiva que teria como caminho a realização das principais competições mundiais em solo brasileiro. A partir disso, vimos já há algum tempo, (Guirra & Castellani Filho, 2014, 2015, 2016) buscando abordar uma dessas competições, os V Jogos Mundiais Militares, realizado no Brasil, no ano de 2011, na cidade do Rio de Janeiro. Em tais estudos, nos dedicamos a apontar quais seriam as possíveis razões para a escolha de um país sem nenhuma tradição no esporte fardado em sediar uma competição de tamanhas proporções e que a realização do megaevento militar em solo brasileiro, muito mais do que atender a uma política esportiva, representava os interesses dos atores envolvidos em sua realização: o Governo Federal, o COB e as Forças Armadas, tendo, então, como meta os Jogos Olímpicos de Verão, em 2016. Dois dos objetivos vistos como legados mereceram especial atenção: o primeiro foi a incorporação de atletas civis ás Forças Armadas, por meio da criação do Programa Atletas de Alto Rendimento – PAAR -, no ano de 2009, e o segundo o Programa Forças no Esporte. Delineia-se aqui o objetivo deste estudo, qual seja, mostrar que a participação dos militares em importantes programas esportivos do Governo Federal, como o Programa Forças no Esporte – Segundo Tempo -, também serviu de instância de sustentação do esporte olímpico nacional e meio de expansão do esporte de alto rendimento, derrubando a tese de sua utilização como elemento de democratização do esporte.Mesa 3: Educación Física e Investigación.Facultad de Humanidades y Ciencias de la Educació