Whey protein does not enhance the adaptations to elbow flexor resistance training

Purpose: It is unclear whether protein supplementation augments the gains in muscle strength and size observed after resistance training (RT) because limitations to previous studies include small cohorts, imprecise measures of muscle size and strength, and no control of prior exercise or habitual protein intake. We aimed to determine whether whey protein supplementation affected RT-induced changes in elbow flexor muscle strength and size. Methods: We pair-matched 33 previously untrained, healthy young men for their habitual protein intake and strength response to 3-wk RT without nutritional supplementation (followed by 6 wk of no training) and then randomly assigned them to protein (PRO, n = 17) or placebo (PLA, n = 16) groups. Participants subsequently performed elbow flexor RT 3 d/wk for 12 wk and consumed PRO or PLA immediately before and after each training session. We assessed elbow flexor muscle strength (unilateral 1-repetition maximum and isometric maximum voluntary force) and size (total volume and maximum anatomical cross-sectional area determined with magnetic resonance imaging) before and after the 12-wk RT. Results: PRO and PLA demonstrated similar increases in muscle volume (PRO 17.0% ± 7.1% vs PLA 14.9% ± 4.6%, P = 0.32), anatomical cross-sectional area (PRO 16.2% ± 7.1% vs PLA 15.6% ± 4.4%, P = 0.80), 1-repetition maximum (PRO 41.8% ± 21.2% vs PLA 41.4% ± 19.9%, P = 0.97), and maximum voluntary force (PRO 12.0% ± 9.9% vs PLA 14.5% ± 8.3%, P = 0.43). Conclusions: In the context of this study, protein supplementation did not augment elbow flexor muscle strength and size changes that occurred after 12 wk of RT. © 2012 by the American College of Sports Medicine

A Dictyostelium chalone uses G proteins to regulate proliferation

<p>Abstract</p> <p>Background</p> <p>Several studies have shown that organ size, and the proliferation of tumor metastases, may be regulated by negative feedback loops in which autocrine secreted factors called chalones inhibit proliferation. However, very little is known about chalones, and how cells sense them. We previously identified two secreted proteins, AprA and CfaD, which act as chalones in <it>Dictyostelium</it>. Cells lacking AprA or CfaD proliferate faster than wild-type cells, and adding recombinant AprA or CfaD to cells slows their proliferation.</p> <p>Results</p> <p>We show here that cells lacking the G protein components Galpha8, Galpha9, and Gbeta proliferate faster than wild-type cells despite secreting normal or high levels of AprA and CfaD. Compared with wild-type cells, the proliferation of <it>galpha8^-</it>, <it>galpha9^-</it>and <it>gbeta^-</it>cells are only weakly inhibited by recombinant AprA (rAprA). Like AprA and CfaD, Galpha8 and Gbeta inhibit cell proliferation but not cell growth (the rate of increase in mass and protein per nucleus), whereas Galpha9 inhibits both proliferation and growth. <it>galpha8^-</it>cells show normal cell-surface binding of rAprA, whereas <it>galpha9^-</it>and <it>gbeta^-</it>cells have fewer cell-surface rAprA binding sites, suggesting that Galpha9 and Gbeta regulate the synthesis or processing of the AprA receptor. Like other ligands that activate G proteins, rAprA induces the binding of [³H]GTP to membranes, and GTPgammaS inhibits the binding of rAprA to membranes. Both AprA-induced [³H]GTP binding and the GTPgammaS inhibition of rAprA binding require Galpha8 and Gbeta but not Galpha9. Like <it>aprA^-</it>cells, <it>galpha8^-</it>cells have reduced spore viability.</p> <p>Conclusion</p> <p>This study shows that Galpha8 and Gbeta are part of the signal transduction pathway used by AprA to inhibit proliferation but not growth in <it>Dictyostelium</it>, whereas Galpha9 is part of a differealnt pathway that regulates both proliferation and growth, and that a chalone signal transduction pathway uses G proteins.</p

How do older people with sight loss manage their general health? A qualitative study

Purpose: Older people with sight loss experience a number of barriers to managing their health. The purpose of this qualitative study was to explore how older people with sight loss manage their general health and explore the techniques used and strategies employed for health management. Methods: Semi-structured face-to-face interviews were conducted with 30 participants. Interviews were audio-recorded, transcribed verbatim and analysed using thematic analysis. Results: Health management challenges experienced included: managing multiple health conditions; accessing information; engaging in health behaviours and maintaining wellbeing. Positive strategies included: joining support groups, clubs and societies; using low vision aids; seeking support from family and friends and accessing support through health and social care services. Conclusion: Healthcare professionals need to be more aware of the challenges faced by older people with sight loss. Improved promotion of group support and charity services which are best placed to share information, provide fora to learn about coping techniques and strategies, and give older people social support to prevent isolation is needed. Rehabilitation and support services and equipment can only be beneficial if patients know what is available and how to access them. Over-reliance on self-advocacy in current healthcare systems is not conducive to patient-centred care. Implications for Rehabilitation Sight loss in older people can impact on many factors including health management. This study identifies challenges to health management and highlights strategies used by older people with sight loss to manage their health. Access to support often relies on patients seeking information for themselves. However, self-advocacy is challenging due to information accessibility barriers. Informal groups and charities play an important role in educating patients about their condition and advising on available support to facilitate health management

Dewar Benzenoids Discovered in Carbon Nanobelts

© 2020 American Chemical Society. The synthesis of cyclacene nanobelts remains an elusive goal dating back over 60 years. These molecules represent the last unsynthesized building block of carbon nanotubes and may be useful both as seed molecules for the preparation of structurally well-defined carbon nanotubes and for understanding the behavior and formation of zigzag nanotubes more broadly. Here we report the discovery that isomers containing two Dewar benzenoid rings are the preferred form for several sizes of cyclacene. The predicted lower polyradical character and higher singlet-triplet stability that these isomers possess compared with their pure benzenoid counterparts suggest that they may be more stable synthetic targets than the structures that have previously been identified. Our findings should facilitate the exploration of new routes to cyclacene synthesis through Dewar benzene chemistry

Dispersed Repetitive DNA Has Spread to New Genomes Since Polyploid Formation in Cotton

Polyploid formation has played a major role in the evolution of many plant and animal genomes; however, surprisingly little is known regarding the subsequent evolution of DNA sequences that become newly united in a common nucleus. Of particular interest is the repetitive DNA fraction, which accounts for most nuclear DNA in higher plants and animals and which can be remarkably different, even in closely related taxa. In one recently formed polyploid, cotton (Gossypium barbadense L.; AD genome), 83 non-cross-hybridizing DNA clones contain dispersed repeats that are estimated to comprise about 24% of the nuclear DNA. Among these, 64 (77%) are largely restricted to diploid taxa containing the larger A genome and collectively account for about half of the difference in DNA content between Old World (A) and New World (D) diploid ancestors of cultivated AD tetraploid cotton. In tetraploid cotton, FISH analysis showed that some A-genome dispersed repeats appear to have spread to D-genome chromosomes. Such spread may also account for the finding that one, and only one, D-genome diploid cotton, Gossypium gossypioides, contains moderate levels of (otherwise) A-genome-specific repeats in addition to normal levels of D-genome repeats. The discovery of A-genome repeats in G. gossypioides adds genome-wide support to a suggestion previously based on evidence from only a single genetic locus that this species may be either the closest living descendant of the New World cotton ancestor, or an adulterated relic of polyploid formation. Spread of dispersed repeats in the early stages of polyploid formation may provide a tag to identify diploid progenitors of a polyploid. Although most repetitive clones do not correspond to known DNA sequences, 4 correspond to known transposons, most contain internal subrepeats, and at least 12 (including 2 of the possible transposons) hybridize to mRNAs expressed at readily discernible levels in cotton seedlings, implicating transposition as one possible mechanism of spread. Integration of molecular, phylogenetic, and cytogenetic analysis of dispersed repetitive DNA may shed new light on evolution of other polyploid genomes, as well as providing valuable landmarks for many aspects of genome analysis

Galactic-Scale Outflow and Supersonic Ram-Pressure Stripping in the Virgo Cluster Galaxy NGC 4388

The Hawaii Imaging Fabry-Perot Interferometer (HIFI) on the University of Hawaii 2.2m telescope was used to map the Halpha and [O III] 5007 A emission-line profiles across the entire disk of the edge-on Sb galaxy NGC 4388. We confirm a rich complex of highly ionized gas that extends ~4 kpc above the disk of this galaxy. Low-ionization gas associated with star formation is also present in the disk. Evidence for bar streaming is detected in the disk component and is discussed in a companion paper (Veilleux, Bland-Hawthorn, & Cecil 1999; hereafter VBC). Non-rotational blueshifted velocities of 50 - 250 km/s are measured in the extraplanar gas north-east of the nucleus. The brighter features in this complex tend to have more blueshifted velocities. A redshifted cloud is also detected 2 kpc south-west of the nucleus. The velocity field of the extraplanar gas of NGC 4388 appears to be unaffected by the inferred supersonic (Mach number M ~ 3) motion of this galaxy through the ICM of the Virgo cluster. We argue that this is because the galaxy and the high-|z| gas lie behind a Mach cone with opening angle ~ 80 degrees. The shocked ICM that flows near the galaxy has a velocity of ~ 500 km/s and exerts insufficient ram pressure on the extraplanar gas to perturb its kinematics. We consider several explanations of the velocity field of the extraplanar gas. Velocities, especially blueshifted velocities on the N side of the galaxy, are best explained as a bipolar outflow which is tilted by > 12 degrees from the normal to the disk. The observed offset between the extraplanar gas and the radio structure may be due to buoyancy or refractive bending by density gradients in the halo gas. Velocity substructure in the outflowing gas also suggests an interaction with ambient halo gas.Comment: 29 pages including 5 figures, Latex, requires aaspp4.sty, to appear in ApJ, 520 (July 20, 1999 issue

The Luminosity Function and Color-Magnitude Diagram of the Globular Cluster M12

In this paper we present the V and I luminosity functions and color-magnitude diagrams derived from wide-field (23 arcmin by 23 arcmin) BVI photometry of the intermediate metallicity ([Fe/H]=-1.3) Galactic globular cluster M12. Using observed values (and ranges of values) for the cluster metallicity, reddening, distance modulus, and age we compare these data to recent alpha-enhanced stellar evolution models for low mass metal-poor stars. We describe several methods of making comparisons between theoretical and observed luminosity functions in order to isolate the evolutionary timescale information the luminosity functions contain. We find no significant evidence of excesses of stars on the red giant branch, although the morphology of the subgiant branch in the observed luminosity function does not match theoretical predictions in a satisfactory way. Current uncertainties in Teff-color transformations (and possibly also in other physics inputs to the models) make more detailed conclusions about the subgiant branch morphology impossible. Given the recent constraints on cluster ages from the WMAP experiment (Spergel et al. 2003), we find that good fitting models that do not include He diffusion (both color-magnitude diagrams and luminosity functions) are too old (by approximately 1-2 Gyr) to adequately represent the cluster luminosity function. The inclusion of helium diffusion in the models provides an age reduction (compared to non-diffusive models) that is consistent with the age of the universe being 13.7+/-0.2 Gyr (Bennett et al. 2003).Comment: 63 pages, 29 figures, accepted for ApJ. Quality of images are degraded; please e-mail lead author for high-quality PS/PDF preprin

Remembering Our Past: Teaching the History of Anatomy at Indiana University

Most students pursuing careers in anatomy or related disciplines have a limited understanding of how, over the centuries, the intricate structure of the human body came to be known. To provide students with the relevant historical perspective, we developed a team-taught survey course in the history of anatomical sciences—including gross anatomy, histology, neuroanatomy, and embryology—from antiquity to the present. Taught entirely via Zoom during the Spring semester of 2021, History of Anatomy (2 semester hours credit) met once per week for approximately 2 hours. Enrollment consisted of 5 undergraduate students majoring in Biology (2), Human Biology (2), or Anthropology (1), as well as 3 graduate students pursuing either a master’s degree in Clinical Anatomy (1) or a Ph.D. in Anatomy Education (2). Three of the students had no prior coursework in anatomy. Through assigned readings, lectures, and discussions, the class explored the work of the great anatomists and their discoveries. A particular emphasis was placed on the evolution of anatomy as a discipline and the cultural influences, scientific controversies, and ethical dilemmas facing its practitioners. Syllabus topics included critical appraisals of the role of gender, race, and ethnicity in anatomical discovery. A key feature of the course was the opportunity for students to engage in robust discussions about such controversial issues as: Eurocentric biases in our understanding of human anatomy and the untold story of Muslim contributions to anatomical knowledge well before Vesalius; Competing claims of priority for who “discovered” the pulmonary circulation; The underappreciated role of women in the history of anatomy and medicine; The ethical quandary of teaching anatomy from archival fetal specimens obtained before the era of informed consent; Accusations that famed anatomist William Hunter used the bodies of murdered pregnant women to create his anatomical atlas of the gravid uterus; Complicity of Eduard Pernkopf and other Nazi-era anatomists in the unethical use of executed victims to obtain images for a renowned anatomical atlas. All students were assessed through weekly homework (written responses to study questions), a mid-term writing assignment, and a term paper about an historical topic of the student’s choosing. Graduate students had the additional requirement of a class presentation about their term paper topic. The end-of-course evaluation suggested that the course was well-received by the students (mean Likert score = 4.63 on a 5-point scale; n = 6). Based on this positive reception, we plan to offer History of Anatomy again on a recurring basis. We believe that by knowing our history, both the good and the bad, future practitioners of anatomy and related disciplines will be less likely to perpetuate the biases and ethical transgressions of earlier eras.American Association for Anatomy Spring Meetin

Open Data, Open Source and Open Standards in chemistry: The Blue Obelisk five years on

RIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are.Abstract Background The Blue Obelisk movement was established in 2005 as a response to the lack of Open Data, Open Standards and Open Source (ODOSOS) in chemistry. It aims to make it easier to carry out chemistry research by promoting interoperability between chemistry software, encouraging cooperation between Open Source developers, and developing community resources and Open Standards. Results This contribution looks back on the work carried out by the Blue Obelisk in the past 5 years and surveys progress and remaining challenges in the areas of Open Data, Open Standards, and Open Source in chemistry. Conclusions We show that the Blue Obelisk has been very successful in bringing together researchers and developers with common interests in ODOSOS, leading to development of many useful resources freely available to the chemistry community.Peer Reviewe