8,646 research outputs found

    Using Insights from Psychology and Language to Improve How People Reason with Description Logics

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    Inspired by insights from theories of human reasoning and language, we propose additions to the Manchester OWL Syntax to improve comprehensibility. These additions cover: functional and inverse functional properties, negated conjunction, the definition of exceptions, and existential and universal restrictions. By means of an empirical study, we demonstrate the effectiveness of a number of these additions, in particular: the use of solely to clarify the uniqueness of the object in a functional property; the replacement of and with intersection in conjunction, which was particularly beneficial in negated conjunction; the use of except as a substitute for and not; and the replacement of some with including and only with noneOrOnly, which helped in certain situations to clarify the nature of these restrictions

    The Anatomy of the Internet Meets the Body of the Law

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    Symposium: Copyright Owners\u27 Rights and Users\u27 Privileges on the Interne

    Software Reverse Engineering in the Real World

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    Symposium: Copyright Protection and Reverse Engineering of Softwar

    Inhibition of gap junction and adherens junction assembly by connexin and A-CAM antibodies

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    We examined the roles of the extracellular domains of a gap junction protein and a cell adhesion molecule in gap junction and adherens junction formation by altering cell interactions with antibody Fab fragments. Using immunoblotting and immunocytochemistry we demonstrated that Novikoff cells contained the gap junction protein, connexin43 (Cx43), and the cell adhesion molecule, A-CAM (N-cadherin). Cells were dissociated in EDTA, allowed to recover, and reaggregated for 60 min in media containing Fab fragments prepared from a number of antibodies. We observed no cell-cell dye transfer 4 min after microinjection in 90% of the cell pairs treated with Fab fragments of antibodies for the first or second extracellular domain of Cx43, the second extracellular domain of connexin32 (Cx32) or A-CAM. Cell-cell dye transfer was detected within 30 s in cell pairs treated with control Fab fragments (pre-immune serum, antibodies to the rat major histocompatibility complex or the amino or carboxyl termii of Cx43). We observed no gap junctions by freeze-fracture EM and no adherens junctions by thin section EM between cells treated with the Fab fragments that blocked cell-cell dye transfer. Gap junctions were found on approximately 50% of the cells in control samples using freeze-fracture EM. We demonstrated with reaggregated Novikoff cells that: (a) functional interactions of the extracellular domains of the connexins were necessary for the formation of gap junction channels; (b) cell interactions mediated by A-CAM were required for gap junction assembly; and (c) Fab fragments of antibodies for A-CAM or connexin extracellular domains blocked adherens junction formation
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