14 research outputs found
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“Pregnant? Scared? Overwhelmed? We’re Here to Help:” Cultures of Care In Crisis Pregnancy Centers
Crisis Pregnancy Centers are non-profit, faith-based organizations that operate with the express intent to offer alternatives to abortion. These pregnancy centers comprise the largest component of the pro-life movement in the United States, yet little is know about the interpersonal dynamics that occur within centers and specifically, between staff and clients. Using observations from options counseling and ultrasound appointments, as well as data from interviews with staff and clients, this dissertation presents the first ethnographic data and scholarly analysis of client appointments in faith-based, “life-affirming” pregnancy centers. I focus on the construction, performance, and receipt of care at two pregnancy centers. These two centers provide distinct models of care that reveal important frames through which staff negotiate their gendered, religious identities and provide the context through which to understand how clients experience pregnancy centers. I add to a growing body of literature examining CPCs by contributing the first empirical data documenting the exchanges that occur within appointments at pregnancy centers. I also contribute to the literature that explores women’s participation in evangelical Christianity by examining how staff reimagine effective evangelism and ministry in the pregnancy center context.</p
The multiplex bead array approach to identifying serum biomarkers associated with breast cancer
Introduction Breast cancer is the most common type of cancer seen in women in western countries. Thus, diagnostic modalities sensitive to early-stage breast cancer are needed. Antibody-based array platforms of a data-driven type, which are expected to facilitate more rapid and sensitive detection of novel biomarkers, have emerged as a direct, rapid means for profiling cancer-specific signatures using small samples. In line with this concept, our group constructed an antibody bead array panel for 35 analytes that were selected during the discovery step. This study was aimed at testing the performance of this 35-plex array panel in profiling signatures specific for primary non-metastatic breast cancer and validating its diagnostic utility in this independent population. Methods Thirty-five analytes were selected from more than 50 markers through screening steps using a serum bank consisting of 4,500 samples from various types of cancer. An antibody-bead array of 35 markers was constructed using the Luminex (TM) bead array platform. A study population consisting of 98 breast cancer patients and 96 normal subjects was analysed using this panel. Multivariate classification algorithms were used to find discriminating biomarkers and validated with another independent population of 90 breast cancer and 79 healthy controls. Results Serum concentrations of epidermal growth factor, soluble CD40-ligand and proapolipoprotein A1 were increased in breast cancer patients. High-molecular-weight-kininogen, apolipoprotein A1, soluble vascular cell adhesion molecule-1, plasminogen activator inhibitor-1, vitamin-D binding protein and vitronectin were decreased in the cancer group. Multivariate classification algorithms distinguished breast cancer patients from the normal population with high accuracy (91.8% with random forest, 91.5% with support vector machine, 87.6% with linear discriminant analysis). Combinatorial markers also detected breast cancer at an early stage with greater sensitivity. Conclusions The current study demonstrated the usefulness of the antibody-bead array approach in finding signatures specific for primary non-metastatic breast cancer and illustrated the potential for early, high sensitivity detection of breast cancer. 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A strategy to select suitable physicochemical attributes of amino acids for protein fold recognition
Effect of cultural practices on control of maize chlorotic mottle virus and beetle larvae-virus transmission studies
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