Global mRNA Degradation during Lytic Gammaherpesvirus Infection Contributes to Establishment of Viral Latency

During a lytic gammaherpesvirus infection, host gene expression is severely restricted by the global degradation and altered 3′ end processing of mRNA. This host shutoff phenotype is orchestrated by the viral SOX protein, yet its functional significance to the viral lifecycle has not been elucidated, in part due to the multifunctional nature of SOX. Using an unbiased mutagenesis screen of the murine gammaherpesvirus 68 (MHV68) SOX homolog, we isolated a single amino acid point mutant that is selectively defective in host shutoff activity. Incorporation of this mutation into MHV68 yielded a virus with significantly reduced capacity for mRNA turnover. Unexpectedly, the MHV68 mutant showed little defect during the acute replication phase in the mouse lung. Instead, the virus exhibited attenuation at later stages of in vivo infections suggestive of defects in both trafficking and latency establishment. Specifically, mice intranasally infected with the host shutoff mutant accumulated to lower levels at 10 days post infection in the lymph nodes, failed to develop splenomegaly, and exhibited reduced viral DNA levels and a lower frequency of latently infected splenocytes. Decreased latency establishment was also observed upon infection via the intraperitoneal route. These results highlight for the first time the importance of global mRNA degradation during a gammaherpesvirus infection and link an exclusively lytic phenomenon with downstream latency establishment

Striving for Knowledge and Dignity: How Qur’anic Students in Kano, Nigeria, Learn to Live with Rejection and Educational Disadvantage

What is it like for young people not to conform to increasingly globalised standards of ‘modern childhood’, as epitomised by formal schooling? Drawing on ethnographic and participatory research conducted with Qur’anic students (Almajirai) in Kano, Nigeria, this article explores how young people – excluded from forms of knowledge to which they aspire – struggle to make sense of the constraints upon their lives and futures. It first traces how the Almajiri system evolved from a prestigious avenue to power into a coping strategy for the poor. It then describes the educational policy context of the Almajirai's experiences and explores how, shut out from modern Islamic and secular models of education, they cope with the exclusion and rejection they face in daily life. Throughout the article, the problematic implications of pushing for universal enrolment without addressing the inequalities governing access to education that is meaningful and of acceptable quality are pointed out

Janus’ Voice: Religious Leaders, Framing, and Riots in Kano

This article analyses the role of religious leaders in collective violence in Kano, the major urban centre in northern Nigeria. It compares two episodes of collective action in the city—the violent ‘Plateau riots’ in 2004 and the non-violent ‘cartoon protests’ in 2006—to explore the role of religious leaders in the variation in violence between the two events. The core argument is that the ways in which Islamic and Christian preachers framed the triggering events for these cases facilitated different forms of mobilisation and enemy identification in response. In 2004, the interpretation of violence in Plateau State through the ‘Christians-versus-Muslims’ frame allowed for mobilisation within Kano’s Christian and Muslim communities as well as for the identification of local Christians as enemies. In 2006, in contrast, the infamous Danish cartoons were actively framed as part of the global struggle between faithful Nigerians and nonreligious Westerners, facilitating non-violent mobilisation across Christian-Muslim boundaries. Thus, the divergent discursive strategies employed by religious leaders are likely to have contributed to violent escalation in 2004 and to peaceful mobilisation in 2006. At the same time, however, the article emphasises the interaction of discursive framing with other factors, such as the role of security forces and the inextricable connections between religious and political authorities in Kano. The article is based on mixed-methods data collected in Kano between 2006 and 2012, including perceptions survey data, semi-structured interviews, and newspaper articles.Global Challenges (FGGA