User-steered image segmentation paradigms: Live wire and live lane

In multidimensional image analysis, there are, and will continue to be, situations wherein automatic image segmentation methods fail, calling for considerable user assistance in the process. The main goals of segmentation research for such situations ought to be (i) to provide effective control to the user on the segmentation process while it is being executed, and (ii) to minimize the total user's time required in the process. With these goals in mind, we present in this paper two paradigms, referred to as live wire and live lane, for practical image segmentation in large applications. For both approaches, we think of the pixel vertices and oriented edges as forming a graph, assign a set of features to each oriented edge to characterize its "boundariness," and transform feature values to costs. We provide training facilities and automatic optimal feature and transform selection methods so that these assignments can be made with consistent effectiveness in any application. In live wire, the user first selects an initial point on the boundary. For any subsequent point indicated by the cursor, an optimal path from the initial point to the current point is found and displayed in real time. The user thus has a live wire on hand which is moved by moving the cursor, If the cursor goes close to the boundary, the live wire snaps onto the boundary. At this point, if the live wire describes the boundary appropriately, the user deposits the cursor which now becomes the new starting point and the process continues. A few points (live-wire segments) are usually adequate to segment the whole 2D boundary. in live lane, the user selects only the initial point. Subsequent points are selected automatically as the cursor is moved within a lane surrounding the boundary whose width changes as a function of the speed and acceleration of cursor motion. Live-wire segments are generated and displayed in real time between successive points. The users get the feeling that the curve snaps onto the boundary as and while they roughly mark in the vicinity of the boundary. We describe formal evaluation studies to compare the utility of the new methods with that of manual tracing based on speed and repeatability of tracing and on data taken from a large ongoing application. The studies indicate that the new methods are statistically significantly more repeatable and 1.5-2.5 times faster than manual tracing. (C) 1998 Academic Press.60423326

Artemisinin resistance: current status and scenarios for containment.

Artemisinin combination therapies are the first-line treatments for uncomplicated Plasmodium falciparum malaria in most malaria-endemic countries. Recently, partial artemisinin-resistant P. falciparum malaria has emerged on the Cambodia-Thailand border. Exposure of the parasite population to artemisinin monotherapies in subtherapeutic doses for over 30 years, and the availability of substandard artemisinins, have probably been the main driving force in the selection of the resistant phenotype in the region. A multifaceted containment programme has recently been launched, including early diagnosis and appropriate treatment, decreasing drug pressure, optimising vector control, targeting the mobile population, strengthening management and surveillance systems, and operational research. Mathematical modelling can be a useful tool to evaluate possible strategies for containment

Essential Oils from Aromatic and Medicinal Plants as Effective Weapons Against Mosquito Vectors of Public Health Importance

The fight against mosquito-borne diseases has recently seen the failure of control programmes based on synthetic chemical treatments to combat larvae and adults of mosquito vectors. This has led to several problems linked to residual substances causing a detrimental impact on environment and human health and to the development of resistance in mosquitoes. In this scenario, new eco-friendly and alternative strategies for the management of mosquito-borne diseases come from the use of plant essential oils (EOs). These are complex mixtures of small, volatile and lipophilic compounds, mostly belonging to monoterpenoids, sesquiterpenoids and phenylpropanoids, produced by aromatic plants belonging to several botanical families such as Apiaceae, Asteraceae, Geraniaceae, Lamiaceae, Lauraceae, Myrtaceae, Poaceae, Rutaceae, Verbenaceae and Zingiberaceae. An important ecological role played by EOs is defending plants from several enemies such as bacterial and fungal pathogens, viruses, insects and parasites. EOs represent ideal candidate ingredients to be incorporated in insecticidal formulations since scientific evidences have documented their efficacy against larvae and adults of several mosquitoes (e.g. Anopheles, Aedes and Culex) even at low doses (<50 ppm), the multiple mode of action and wide spectrum of efficacy, the low toxicity on nontarget organisms and environment and the unlikely capacity to induce insect resistance. In this chapter, we gave an overview of the most important EOs obtained from commercially important botanical families with documented efficacy against mosquito vectors. Particular attention has been paid to highlight their strengths and weakness and the future challenges leading to the replacement of conventional insecticides by agrochemical companies