Open defecation and childhood stunting in India: an ecological analysis of new data from 112 districts.

Poor sanitation remains a major public health concern linked to several important health outcomes; emerging evidence indicates a link to childhood stunting. In India over half of the population defecates in the open; the prevalence of stunting remains very high. Recently published data on levels of stunting in 112 districts of India provide an opportunity to explore the relationship between levels of open defecation and stunting within this population. We conducted an ecological regression analysis to assess the association between the prevalence of open defecation and stunting after adjustment for potential confounding factors. Data from the 2011 HUNGaMA survey was used for the outcome of interest, stunting; data from the 2011 Indian Census for the same districts was used for the exposure of interest, open defecation. After adjustment for various potential confounding factors--including socio-economic status, maternal education and calorie availability--a 10 percent increase in open defecation was associated with a 0.7 percentage point increase in both stunting and severe stunting. Differences in open defecation can statistically account for 35 to 55 percent of the average difference in stunting between districts identified as low-performing and high-performing in the HUNGaMA data. In addition, using a Monte Carlo simulation, we explored the effect on statistical power of the common practice of dichotomizing continuous height data into binary stunting indicators. Our simulation showed that dichotomization of height sacrifices statistical power, suggesting that our estimate of the association between open defecation and stunting may be a lower bound. Whilst our analysis is ecological and therefore vulnerable to residual confounding, these findings use the most recently collected large-scale data from India to add to a growing body of suggestive evidence for an effect of poor sanitation on human growth. New intervention studies, currently underway, may shed more light on this important issue

The logic of kinetic regulation in the thioredoxin system

<p>Abstract</p> <p>Background</p> <p>The thioredoxin system consisting of NADP(H), thioredoxin reductase and thioredoxin provides reducing equivalents to a large and diverse array of cellular processes. Despite a great deal of information on the kinetics of individual thioredoxin-dependent reactions, the kinetic regulation of this system as an integrated whole is not known. We address this by using kinetic modeling to identify and describe kinetic behavioral motifs found within the system.</p> <p>Results</p> <p>Analysis of a realistic computational model of the <it>Escherichia coli </it>thioredoxin system revealed several modes of kinetic regulation in the system. In keeping with published findings, the model showed that thioredoxin-dependent reactions were adaptable (i.e. changes to the thioredoxin system affected the kinetic profiles of these reactions). Further and in contrast to other systems-level descriptions, analysis of the model showed that apparently unrelated thioredoxin oxidation reactions can affect each other via their combined effects on the thioredoxin redox cycle. However, the scale of these effects depended on the kinetics of the individual thioredoxin oxidation reactions with some reactions more sensitive to changes in the thioredoxin cycle and others, such as the Tpx-dependent reduction of hydrogen peroxide, less sensitive to these changes. The coupling of the thioredoxin and Tpx redox cycles also allowed for ultrasensitive changes in the thioredoxin concentration in response to changes in the thioredoxin reductase concentration. We were able to describe the kinetic mechanisms underlying these behaviors precisely with analytical solutions and core models.</p> <p>Conclusions</p> <p>Using kinetic modeling we have revealed the logic that underlies the functional organization and kinetic behavior of the thioredoxin system. The thioredoxin redox cycle and associated reactions allows for a system that is adaptable, interconnected and able to display differential sensitivities to changes in this redox cycle. This work provides a theoretical, systems-biological basis for an experimental analysis of the thioredoxin system and its associated reactions.</p

Multilocus Phylogenetic Study of the Scheffersomyces Yeast Clade and Characterization of the N-Terminal Region of Xylose Reductase Gene

Many of the known xylose-fermenting (X-F) yeasts are placed in the Scheffersomyces clade, a group of ascomycete yeasts that have been isolated from plant tissues and in association with lignicolous insects. We formally recognize fourteen species in this clade based on a maximum likelihood (ML) phylogenetic analysis using a multilocus dataset. This clade is divided into three subclades, each of which exhibits the biochemical ability to ferment cellobiose or xylose. New combinations are made for seven species of Candida in the clade, and three X-F taxa associated with rotted hardwood are described: Scheffersomyces illinoinensis (type strain NRRL Y-48827T  =  CBS 12624), Scheffersomyces quercinus (type strain NRRL Y-48825T  =  CBS 12625), and Scheffersomyces virginianus (type strain NRRL Y-48822T  =  CBS 12626). The new X-F species are distinctive based on their position in the multilocus phylogenetic analysis and biochemical and morphological characters. The molecular characterization of xylose reductase (XR) indicates that the regions surrounding the conserved domain contain mutations that may enhance the performance of the enzyme in X-F yeasts. The phylogenetic reconstruction using XYL1 or RPB1 was identical to the multilocus analysis, and these loci have potential for rapid identification of cryptic species in this clade

Reaction Rate Expressions for the reaction of CO<SUB>2</SUB> and Mono-ethanolamine in a 2-Propanol Solvent System.

Please help us populate SUNScholar with the post print version of this article. It can be e-mailed to: [email protected]

Absorption Rates of CO<SUB>2</SUB> into Solutions of Mono-ethanolamine/2-Propanol.

Please help us populate SUNScholar with the post print version of this article. It can be e-mailed to: [email protected]

Evaluating Reaction Rate Expressions for CO<SUB>2</SUB> and MEA in 2-Propanol.

Please help us populate SUNScholar with the post print version of this article. It can be e-mailed to: [email protected]

Canola (Brassica napus L.) water use indicators as affected by sustained deficit irrigation and plant density in central Free State, South Africa

In South Africa canola (Brassica napus L.) is cultivated in rotation with wheat under winter rainfall in the Western Cape Province, primarily for seed to make oil. Expansion of the crop to the other 8 provinces is proposed to reduce shortages of locally produced plant oils. At the same time, canola can serve as a rotational crop for wheat in these summer rainfall provinces. In central Free State, information on evapotranspiration and various water use indicators for canola as influenced by sustained deficit irrigation and plant density is lacking. An experiment with a line source sprinkler irrigation system was therefore conducted, comprising of full irrigation as a control with 4 sustained deficit irrigation levels (mean reduction in irrigation depth per event of 67%, 52%, 34% and 19%) and 5 plant densities (25, 50, 75, 100 and 125 plants‧m−2). Mean seasonal maximum evapotranspiration amounted to 429 mm across plant densities. Plant density did not significantly influence seasonal evapotranspiration. Reducing the irrigation depth per event by more than 20% decreased seasonal evapotranspiration by a mean 3.5 mm per percentage increase in irrigation depth. A maximum biomass water productivity of 22 kg‧ha−1‧mm−1 was measured with full irrigation and a plant density of 75 plants‧m−2. Seed water productivity amounted to a high of 11 kg‧ha−1‧mm−1 with full irrigation and a plant density of 25 plants‧m−2. A percentage reduction in irrigation depth and increase in plant density above 25 plants‧m−2 will reduce seed water productivity by 0.071 and 0.033 kg‧ha−1‧mm−1, respectively. Sustained deficit irrigation increased water use efficiency by a mean 0.5% per percentage reduction in irrigation depth per event