Altered expression of the voltage-gated calcium channel subunit α2δ-1: a comparison between two experimental models of epilepsy and a sensory nerve ligation model of neuropathic pain.

The auxiliary α2δ-1 subunit of voltage-gated calcium channels is up-regulated in dorsal root ganglion neurons following peripheral somatosensory nerve damage, in several animal models of neuropathic pain. The α2δ-1 protein has a mainly presynaptic localization, where it is associated with the calcium channels involved in neurotransmitter release. Relevant to the present study, α2δ-1 has been shown to be the therapeutic target of the gabapentinoid drugs in their alleviation of neuropathic pain. These drugs are also used in the treatment of certain epilepsies. In this study we therefore examined whether the level or distribution of α2δ-1 was altered in the hippocampus following experimental induction of epileptic seizures in rats, using both the kainic acid model of human temporal lobe epilepsy, in which status epilepticus is induced, and the tetanus toxin model in which status epilepticus is not involved. The main finding of this study is that we did not identify somatic overexpression of α2δ-1 in hippocampal neurons in either of the epilepsy models, unlike the upregulation of α2δ-1 that occurs following peripheral nerve damage to both somatosensory and motor neurons. However, we did observe local reorganisation of α2δ-1 immunostaining in the hippocampus only in the kainic acid model, where it was associated with areas of neuronal cell loss, as indicated by absence of NeuN immunostaining, dendritic loss, as identified by areas where microtubule-associated protein-2 immunostaining was missing, and reactive gliosis, determined by regions of strong OX42 staining

An m sin i = 24 Earth Mass Planetary Companion To The Nearby M Dwarf GJ 176

We report the detection of a planetary companion with a minimum mass of m sin i = 0.0771 M_Jup = 24.5 M_Earth to the nearby (d = 9.4 pc) M2.5V star GJ 176. The star was observed as part of our M dwarf planet search at the Hobby-Eberly Telescope (HET). The detection is based on 5 years of high-precision differential radial velocity (RV) measurements using the High-Resolution-Spectrograph (HRS). The orbital period of the planet is 10.24 d. GJ 176 thus joins the small (but increasing) sample of M dwarfs hosting short-periodic planets with minimum masses in the Neptune-mass range. Low mass planets could be relatively common around M dwarfs and the current detections might represent the tip of a rocky planet population.Comment: 13 pages preprint, 3 figures, submitted to Ap

High-Frequency network activity, global increase in Neuronal Activity, and Synchrony Expansion Precede Epileptic Seizures In Vitro

How seizures start is a major question in epilepsy research. Preictal EEG changes occur in both human patients and animal models, but their underlying mechanisms and relationship with seizure initiation remain unknown. Here we demonstrate the existence, in the hippocampal CA1 region, of a preictal state characterized by the progressive and global increase in neuronal activity associated with a widespread buildup of low-amplitude high-frequency activity (HFA) (100 Hz) and reduction in system complexity.HFAis generated by the firing of neurons, mainly pyramidal cells, at much lower frequencies. Individual cycles ofHFAare generated by the near-synchronous (within 5 ms) firing of small numbers of pyramidal cells. The presence of HFA in the low-calcium model implicates nonsynaptic synchronization; the presence of very similar HFA in the high-potassium model shows that it does not depend on an absence of synaptic transmission. Immediately before seizure onset, CA1 is in a state of high sensitivity in which weak depolarizing or synchronizing perturbations can trigger seizures. Transition to seizure is haracterized by a rapid expansion and fusion of the neuronal populations responsible for HFA, associated with a progressive slowing of HFA, leading to a single, massive, hypersynchronous cluster generating the high-amplitude low-frequency activity of the seizure

Bayesian model comparison applied to the Explorer-Nautilus 2001 coincidence data

Bayesian reasoning is applied to the data by the ROG Collaboration, in which gravitational wave (g.w.) signals are searched for in a coincidence experiment between Explorer and Nautilus. The use of Bayesian reasoning allows, under well defined hypotheses, even tiny pieces of evidence in favor of each model to be extracted from the data. The combination of the data of several experiments can therefore be performed in an optimal and efficient way. Some models for Galactic sources are considered and, within each model, the experimental result is summarized with the likelihood rescaled to the insensitivity limit value (``${\cal R}$ function''). The model comparison result is given in in terms of Bayes factors, which quantify how the ratio of beliefs about two alternative models are modified by the experimental observationComment: 16 pages, 4 figures. Presented at the GWDAW2002 conference, held in Kyoto on Dec.,2002. This version includes comments by the referees of CQG, which has accepted the paper for pubblication in the special issue of the conference. In particular, note that in Eq. 12 there was a typeset error. As suggested by one of the referees, a uniform prior in Log(alpha) has also been considere

A Planetary Companion to gamma Cephei A

We report on the detection of a planetary companion in orbit around the primary star of the binary system $\gamma$ Cephei. High precision radial velocity measurements using 4 independent data sets spanning the time interval 1981--2002 reveal long-lived residual radial velocity variations superimposed on the binary orbit that are coherent in phase and amplitude with a period or 2.48 years (906 days) and a semi-amplitude of 27.5 m s$^{-1}$. We performed a careful analysis of our Ca II H & K S-index measurements, spectral line bisectors, and {\it Hipparcos} photometry. We found no significant variations in these quantities with the 906-d period. We also re-analyzed the Ca II $\lambda$8662 {\AA} measurements of Walker et al. (1992) which showed possible periodic variations with the ``planet'' period when first published. This analysis shows that periodic Ca II equivalent width variations were only present during 1986.5 -- 1992 and absent during 1981--1986.5. Furthermore, a refined period for the Ca II $\lambda$8662 {\AA} variations is 2.14 yrs, significantly less than residual radial velocity period. The most likely explanation of the residual radial velocity variations is a planetary mass companion with $M$ sin $i$ = 1.7 $M_{Jupiter}$ and an orbital semi-major axis of $a_2$ $=$ 2.13 AU. This supports the planet hypothesis for the residual radial velocity variations for $\gamma$ Cep first suggested by Walker et al. (1992). With an estimated binary orbital period of 57 years $\gamma$ Cep is the shortest period binary system in which an extrasolar planet has been found. This system may provide insights into the relationship between planetary and binary star formation.Comment: 19 pages, 15 figures, accepted in Ap. J. Includes additional data and improved orbital solutio

A variable star survey of the open cluster M37

A CCD photometric study of the dense galactic open cluster M37 is presented and discussed. The majority of the analysed data are time-series measurements obtained through an R_C filter. The observations were carried out on seven nights between December 1999 and February 2000, and have led to the discovery of 7 new variable stars in the field. Three of them have been unambiguously identified as W UMa-type eclipsing binaries, while two more are monoperiodic pulsating stars, most probably high-amplitude delta Scuti-type variables. The remaining two stars seem to be long-period eclipsing binaries without firm period determination. Johnson B and V frames have been used to construct a new colour-magnitude (CM) diagram of the cluster, and to find the locations of the new variable stars. The pulsating variables are most likely background objects. The CM diagram is fitted with recent isochrones yielding the main parameters of the cluster.Comment: 7 pages, 6 figures, accepted for publication in A&

Deep Photometry of the Globular Cluster M5: Distance Estimates from White Dwarf and Main Sequence Stars

We present deep VI photometry of stars in the globular cluster M5 (NGC 5904) based on images taken with the Hubble Space Telescope. The resulting color-magnitude diagram reaches below V ~ 27 mag, revealing the upper 2-3 magnitudes of the white dwarf cooling sequence, and main sequence stars eight magnitudes and more below the turn-off. We fit the main sequence to subdwarfs of known parallax to obtain a true distance modulus of (m-M)_0 = 14.45 +/- 0.11 mag. A second distance estimate based on fitting the cluster white dwarf sequence to field white dwarfs with known parallax yielded (m-M)_0 = 14.67 +/- 0.18 mag. We couple our distance estimates with extensive photometry of the cluster's RR Lyrae variables to provide a calibration of the RR Lyrae absolute magnitude yielding M_V(RR) = 0.42 +/- 0.10 mag at [Fe/H] = -1.11 dex. We provide another luminosity calibration in the form of reddening-free Wasenheit functions. Comparison of our calibrations with predictions based on recent models combining stellar evolution and pulsation theories shows encouraging agreement. (Abridged)Comment: AASTeX, 29 pages including 5 figures. Complete photometry data and FITS-format images are available at http://physics.bgsu.edu/~layden/ASTRO/PUBL/published.html . Accepted for publication in the Astrophysical Journal, 2005 October 20. Replaced errant wording in last sentence of paragraph 4 of conclusion

Central Masses and Broad-Line Region Sizes of Active Galactic Nuclei. II. A Homogeneous Analysis of a Large Reverberation-Mapping Database

We present improved black hole masses for 35 active galactic nuclei (AGNs) based on a complete and consistent reanalysis of broad emission-line reverberation-mapping data. From objects with multiple line measurements, we find that the highest precision measure of the virial product is obtained by using the cross-correlation function centroid (as opposed to the cross-correlation function peak) for the time delay and the line dispersion (as opposed to full width half maximum) for the line width and by measuring the line width in the variable part of the spectrum. Accurate line-width measurement depends critically on avoiding contaminating features, in particular the narrow components of the emission lines. We find that the precision (or random component of the error) of reverberation-based black hole mass measurements is typically around 30%, comparable to the precision attained in measurement of black hole masses in quiescent galaxies by gas or stellar dynamical methods. Based on results presented in a companion paper by Onken et al., we provide a zero-point calibration for the reverberation-based black hole mass scale by using the relationship between black hole mass and host-galaxy bulge velocity dispersion. The scatter around this relationship implies that the typical systematic uncertainties in reverberation-based black hole masses are smaller than a factor of three. We present a preliminary version of a mass-luminosity relationship that is much better defined than any previous attempt. Scatter about the mass-luminosity relationship for these AGNs appears to be real and could be correlated with either Eddington ratio or object inclination.Comment: 61 pages, including 8 Tables and 16 Figures. Accepted for publication in The Astrophysical Journa