The association between homocysteine in the follicular fluid with embryo quality and pregnancy rate in assisted reproductive techniques

PURPOSE: To investigate the association between follicular fluid homocysteine levels and embryo quality and pregnancy rates in patients undergoing assisted reproduction. METHODS: Fifty infertile women who were admitted to our clinic were enrolled in the study. Ovulation induction was performed by using GnRH agonist and gonadotropins. For each patient, homocysteine level in the follicular fluid was measured by using nephelometric method after the oocyte pick-up. The association between the homocysteine concentration in the follicular fluid and the oocyte–embryo quality, pregnancy rates and hormone levels were investigated. RESULTS: Mean ± SD Hcy was 9.6 ± 2.02 μmol/L and 14.9 ± 2.93 μmol/L in pregnant and non-pregnant women, respectively (p < 0.0001). There were no statistically significant differences between pregnant and non-pregnant women in mean age, duration of infertility, body mass index, the oocyte–embryo quality parameters, and hormone levels. Homocystein did not have any correlation with M2, late M2, and total number of oocytes, number of fertilized oocytes and transferred embryos, and embryo quality grade. Area under curve (AUC) of hcy for prediction of pregnancy failure was 0.922 (p = 0.0001, 95% Confidence interval 0.85–0.99). A threshold of 11.9 μmol/L of hcy had a sensitivity of 82%, specificity of 100%, positive predictive value of 100% and negative predictive value of 91.6% for prediction of pregnancy failure. The subgroup analysis in male factor infertility group (n = 28), showed that mean homocystein was 9.9 ± 2.44 μmol/L and 14.1 ± 2.72 μmol/L in pregnant and non-pregnant women, respectively (p = 0.002). CONCLUSION: Low follicular fluid homocysteine level is associated with a better chance of clinical pregnancy

Actin acting at the Golgi

The organization, assembly and remodeling of the actin cytoskeleton provide force and tracks for a variety of (endo)membrane-associated events such as membrane trafficking. This review illustrates in different cellular models how actin and many of its numerous binding and regulatory proteins (actin and co-workers) participate in the structural organization of the Golgi apparatus and in traf- ficking-associated processes such as sorting, biogenesis and motion of Golgi-derived transport carriers

Negotiation as a cooperative game

Game theory provides us with a set of important methodologies for the study of group decisions as well as negotiation processes. Cooperative game theory is a subfield of game theory that focuses on interactions in which involved parties have the power to make binding agreements. Many group decision and negotiation processes (such as legal arbitrations) fall into this category, and as such, they have been central in the development of cooperative game theory. Particularly, an area of cooperative game theory, called bargaining theory, focuses on bilateral negotiations as well as negotiation processes where coalition formation is not a central concern. The object of study in bargaining theory is a (bargaining) rule, which provides a solution to each bargaining problem (or in other words, negotiation). Studies on bargaining theory employ the axiomatic method to evaluate bargaining rules. This chapter reviews and summarizes several such studies. After a discussion of the bargaining model, we present the important bargaining rules in the literature (including the Nash bargaining rule), as well as the central axioms that characterize them. Next, we discuss strategic issues related to cooperative bargaining, such as the Nash program, implementation of bargaining rules, and games of manipulating bargaining rules. We conclude with a discussion of the recent literature on ordinal bargaining rules