2,794 research outputs found
Interfacial assembly inspired by marine mussels and antifouling effects of polypeptoids : a neutron reflection study
Polypeptoid-coated surfaces and many surface-grafted hydrophilic polymer brushes have been proven efficient in antifouling-the prevention of nonspecific biomolecular adsorption and cell attachment. Protein adsorption, in particular, is known to mediate subsequent cell-surface interactions. However, the detailed antifouling mechanism of polypeptoid and other polymer brush coatings at the molecular level is not well understood. Moreover, most adsorption studies focus only on measuring a single adsorbed mass value, and few techniques are capable of characterizing the hydrated in situ layer structure of either the antifouling coating or adsorbed proteins. In this study, interfacial assembly of polypeptoid brushes with different chain lengths has been investigated in situ using neutron reflection (NR). Consistent with past simulation results, NR revealed a common two-step structure for grafted polypeptoids consisting of a dense inner region that included a mussel adhesive-inspired oligopeptide for grafting polypeptoid chains and a highly hydrated upper region with very low polymer density (molecular brush). Protein adsorption was studied with human serum albumin (HSA) and fibrinogen (FIB), two common serum proteins of different sizes but similar isoelectric points (IEPs). In contrast to controls, we observed higher resistance by grafted polypeptoid against adsorption of the larger FIB, especially for longer chain lengths. Changing the pH to close to the IEPs of the proteins, which generally promotes adsorption, also did not significantly affect the antifouling effect against FIB, which was corroborated by atomic force microscopy imaging. Moreover, NR enabled characterization of the in situ hydrated layer structures of the polypeptoids together with proteins adsorbed under selected conditions. While adsorption on bare SiO2 controls resulted in surface-induced protein denaturation, this was not observed on polypeptoids. Our current results therefore highlight the detailed in situ view that NR may provide for characterizing protein adsorption on polymer brushes as well as the excellent antifouling behavior of polypeptoids
Near tip strain evolution under cyclic loading
The concept of ratchetting strain as a crack driving force in controlling crack growth has previouslybeen explored at Portsmouth using numerical approaches for nickel-based superalloys. In this paper, we reportthe first experimental observations of the near-tip strain evolution as captured by the Digital Image Correlation(DIC) technique on a compact tension specimen of stainless steel 316L. The evolution of the near-tip strainswith loading cycles was studied whilst the crack tip was maintained stationary. The strains were monitored overthe selected distances from the crack tip for a given number of cycles under an incremental loading regime. Theresults show that strain ratchetting does occur with load cycling, and is particularly evident close to the crack tipand under higher loads. A finite element model has been developed to simulate the experiments and thesimulation results are compared with the DIC measurements
Immobilization of Lipases on Alkyl Silane Modified Magnetic Nanoparticles: Effect of Alkyl Chain Length on Enzyme Activity
Background: Biocatalytic processes often require a full recycling of biocatalysts to optimize economic benefits and
minimize waste disposal. Immobilization of biocatalysts onto particulate carriers has been widely explored as an option to
meet these requirements. However, surface properties often affect the amount of biocatalysts immobilized, their bioactivity
and stability, hampering their wide applications. The aim of this work is to explore how immobilization of lipases onto
magnetite nanoparticles affects their biocatalytic performance under carefully controlled surface modification.
Methodology/Principal Findings: Magnetite nanoparticles, prepared through a co-precipitation method, were coated with
alkyl silanes of different alkyl chain lengths to modulate their surface hydrophobicity. Candida rugosa lipase was then
directly immobilized onto the modified nanoparticles through hydrophobic interaction. Enzyme activity was assessed by
catalytic hydrolysis of p-nitrophenyl acetate. The activity of immobilized lipases was found to increase with increasing chain
length of the alkyl silane. Furthermore, the catalytic activities of lipases immobilized on trimethoxyl octadecyl silane (C18)
modified Fe3O4 were a factor of 2 or more than the values reported from other surface immobilized systems. After 7
recycles, the activities of the lipases immobilized on C18 modified nanoparticles retained 65%, indicating significant
enhancement of stability as well through hydrophobic interaction. Lipase immobilized magnetic nanoparticles facilitated
easy separation and recycling with high activity retaining.
Conclusions/Significance: The activity of immobilized lipases increased with increasing alkyl chain length of the alkyl
trimethoxy silanes used in the surface modification of magnetite nanoparticles. Lipase stability was also improved through
hydrophobic interaction. Alkyl silane modified magnetite nanoparticles are thus highly attractive carriers for enzyme
immobilization enabling efficient enzyme recovery and recycling
Multi-layered Ruthenium-modified Bond Coats for Thermal Barrier Coatings
Diffusional approaches for fabrication of multi-layered Ru-modified bond coats for thermal
barrier coatings have been developed via low activity chemical vapor deposition and high activity
pack aluminization. Both processes yield bond coats comprising two distinct B2 layers, based on
NiAl and RuAl, however, the position of these layers relative to the bond coat surface is reversed
when switching processes. The structural evolution of each coating at various stages of the
fabrication process has been and subsequent cyclic oxidation is presented, and the relevant
interdiffusion and phase equilibria issues in are discussed. Evaluation of the oxidation behavior of
these Ru-modified bond coat structures reveals that each B2 interlayer arrangement leads to the
formation of α-Al 2 O 3 TGO at 1100°C, but the durability of the TGO is somewhat different and in
need of further improvement in both cases
Bulk experimental evidence of half-metallic ferromagnetism in doped manganites
We report precise measurements and quantitative data analysis on the
low-temperature resistivity of several ferromagnetic manganite films. We
clearly show that there exists a T^{4.5} term in low-temperature resistivity,
and that this term is in quantitative agreement with the quantum theory of
two-magnon scattering for half metallic ferromagnets. Our present results
provide the first bulk experimental evidence of half-metallic ferromagnetism in
doped manganites.Comment: 4 pages, 4 figure
K^+ -> pi^+pi^0e^+e^-: a novel short-distance probe
We study the decay K^+ -> pi^+ pi^0 e^+ e^-, currently under analysis by the
NA62 Collaboration at CERN. In particular, we provide a detailed analysis of
the Dalitz plot for the long-distance, gamma^*-mediated, contributions
(Bremsstrahlung, direct emission and its interference). We also examine a set
of asymmetries to isolate genuine short-distance effects. While we show that
charge asymmetries are not required to test short distances, they provide the
best environment for its detection. This constitutes by itself a strong
motivation for NA62 to study K^- decays in the future. We therefore provide a
detailed study of different charge asymmetries and the corresponding estimated
signals. Whenever possible, we make contact with the related processes K^+ ->
pi^+ pi^0 gamma and K_L -> pi^+ pi^- e^+ e^- and discuss the advantages of K^+
-> pi^+ pi^0 e^+ e^- over them.Comment: 25 pages, 6 figure
Patterning the neuronal cells via inkjet printing of self-assembled peptides on silk scaffolds
The patterning of neuronal cells and guiding neurite growth are important for neuron tissue engineering and cell-based biosensors. In this paper, inkjet printing has been employed to pattern self-assembled I3QGK peptide nanofibers on silk substrates for guiding the growth of neuron-like PC12 cells. Atomic force microscopy (AFM) confirmed the dynamic self-assembly of I3QGK into nanofiber structures. The printed self-assembled peptide strongly adheres to regenerated silk fibroin (RSF) substrates through charge-charge interactions. It was observed that in the absence of I3QGK, PC12 cells exhibited poor attachment to RSF films, while for RSF surfaces coated or printed with peptide nanofibers, cellular attachment was significantly improved in terms of both cell density and morphology. AFM results revealed that peptide nanofibers can promote the generation of axons and terminal buttons of PC12 cells, indicating that I3QGK nanofibers not only promote cellular attachment but also facilitate differentiation into neuronal phenotypes. Inkjet printing allows complex patterning of peptide nanofibers onto RSF substrates, which enabled us to engineer cell alignment and provide an opportunity to direct axonal development in vitro. The live/dead assay showed that printed I3QGK patterns exhibit no cytotoxicity to PC12 cells demonstrating potential for future nerve tissue engineering applications
Weak and strong electronic correlations in Fe superconductors
In this chapter the strength of electronic correlations in the normal phase
of Fe-superconductors is discussed. It will be shown that the agreement between
a wealth of experiments and DFT+DMFT or similar approaches supports a scenario
in which strongly-correlated and weakly-correlated electrons coexist in the
conduction bands of these materials. I will then reverse-engineer the realistic
calculations and justify this scenario in terms of simpler behaviors easily
interpreted through model results. All pieces come together to show that Hund's
coupling, besides being responsible for the electronic correlations even in
absence of a strong Coulomb repulsion is also the origin of a subtle emergent
behavior: orbital decoupling. Indeed Hund's exchange decouples the charge
excitations in the different Iron orbitals involved in the conduction bands
thus causing an independent tuning of the degree of electronic correlation in
each one of them. The latter becomes sensitive almost only to the offset of the
orbital population from half-filling, where a Mott insulating state is
invariably realized at these interaction strengths. Depending on the difference
in orbital population a different 'Mottness' affects each orbital, and thus
reflects in the conduction bands and in the Fermi surfaces depending on the
orbital content.Comment: Book Chapte
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