Team dynamics in emergency surgery teams: results from a first international survey

Background: Emergency surgery represents a unique context. Trauma teams are often multidisciplinary and need to operate under extreme stress and time constraints, sometimes with no awareness of the trauma\u2019s causes or the patient\u2019s personal and clinical information. In this perspective, the dynamics of how trauma teams function is fundamental to ensuring the best performance and outcomes. Methods: An online survey was conducted among the World Society of Emergency Surgery members in early 2021. 402 fully filled questionnaires on the topics of knowledge translation dynamics and tools, non-technical skills, and difficulties in teamwork were collected. Data were analyzed using the software R, and reported following the Checklist for Reporting Results of Internet E-Surveys (CHERRIES). Results: Findings highlight how several surgeons are still unsure about the meaning and potential of knowledge translation and its mechanisms. Tools like training, clinical guidelines, and non-technical skills are recognized and used in clinical practice. Others, like patients\u2019 and stakeholders\u2019 engagement, are hardly implemented, despite their increasing importance in the modern healthcare scenario. Several difficulties in working as a team are described, including the lack of time, communication, training, trust, and ego. Discussion: Scientific societies should take the lead in offering training and support about the abovementioned topics. Dedicated educational initiatives, practical cases and experiences, workshops and symposia may allow mitigating the difficulties highlighted by the survey\u2019s participants, boosting the performance of emergency teams. Additional investigation of the survey results and its characteristics may lead to more further specific suggestions and potential solutions

Torque– and power–velocity relationships of trunk muscle during isokinetic conditions in chronic low back pain patients

International audienc

Mössbauer study and molecular orbital calculations on the organo-iron (I, II) electron reservoir sandwiches CpFen+ (η6-C6(CH3)6) (n = 0,1) and related CpFe (cyclohexadienyl) complexes

The paramagnetic Fe(I) electron reservoir sandwich η5-C 5H5Fe(I) η6-C6(Me)6, its oxidized mono-cationic diamagnetic form and related diamagnetic η5-C5H 5Fe (cyclohexadienyl) complexes have been studied by Mössbauer spectroscopy on powders and aligned crystals. Spectra under high magnetic field were recorded for powders. On the basis of X-ray data I.E.H.T. MO calculations were performed and gave results in good agreement with experimental data at room temperature and liquid helium temperature. The relaxation of molecular orbitals associated to the electronic reduction from II to I was found to be negligible, which enables to consider a Fe(I) complex as a superposition of a Fe(II) complex and one electron. This electron is occupying the doubly degenerate e *1 orbital and has a very strong metallic character (83 %).Le sandwich paramagnétique réservoir d'électrons η5-C 5H5Fe(I) η6-C6(CH3) 6 ainsi que sa forme diamagnétique monocationique oxydée et les complexes η 5-C5H5Fe (cyclohexadienyl) correspondants ont été étudiés par spectrométrie Mössbauer sur des poudres et des monocristaux. Les spectres sous champ magnétique intense ont été enregistrés sur des poudres. A partir des données de rayons X, on a fait des calculs d'orbitales moléculaires I.E.H.T. qui ont donné des résultats en bon accord avec les données expérimentales à température ambiante et à 4,2 K. Les calculs d'OM montrent que la relaxation des orbitales moléculaires associée à la réduction électronique de II à I est négligeable ce qui permet de considérer un complexe de fer(I) comme la superposition d'un complexe de fer(II) et d'un électron. Cet électron occupe l'orbitale e*1 doublement dégénérée et a un très fort caractère métallique (83 %)

MÖSSBAUER SPECTROSCOPIC STUDY OF 19 ELECTRON ORGANOMETALLIC PARAMAGNETIC SANDWICHES n5-C5R5Fe(I)n6-C6R'6.

No abstract availabl

Selection of reference genes for transcriptional analysis of edible tubers of potato (Solanum tuberosum L.)

Potato (Solanum tuberosum) yield has increased dramatically over the last 50 years and this has been achieved by a combination of improved agronomy and biotechnology efforts. Gene studies are taking place to improve new qualities and develop new cultivars. Reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) is a bench-marking analytical tool for gene expression analysis, but its accuracy is highly dependent on a reliable normalization strategy of an invariant reference genes. For this reason, the goal of this work was to select and validate reference genes for transcriptional analysis of edible tubers of potato. To do so, RT-qPCR primers were designed for ten genes with relatively stable expression in potato tubers as observed in RNA-Seq experiments. Primers were designed across exon boundaries to avoid genomic DNA contamination. Differences were observed in the ranking of candidate genes identified by geNorm, NormFinder and BestKeeper algorithms. The ranks determined by geNorm and NormFinder were very similar and for all samples the most stable candidates were C2, exocyst complex component sec3 (SEC3) and ATCUL3/ATCUL3A/CUL3/CUL3A (CUL3A). According to BestKeeper, the importin alpha and ubiquitin-associated/ts-n genes were the most stable. Three genes were selected as reference genes for potato edible tubers in RT-qPCR studies. The first one, called C2, was selected in common by NormFinder and geNorm, the second one is SEC3, selected by NormFinder, and the third one is CUL3A, selected by geNorm. Appropriate reference genes identified in this work will help to improve the accuracy of gene expression quantification analyses by taking into account differences that may be observed in RNA quality or reverse transcription efficiency across the samples.</p

Valence State Spin Polarization in 3d-3d and 3d-4d Magnetic Materials by Resonant and Non-Resonant X-Ray Emission

Magnetic circular dichroism in X-ray fluorescence from ferromagnetic systems potentially provides unique information on valence state spin polarization. We discuss the use of direct polychromatic radiation from the Helios n helical undulator (97% circular polarization) to excite the Rh 4d valence states. We also show how polychromatic radiation may be used to obtain dichroic resonant fluorescence under standing-wave conditions in magnetic multilayers

Characterization and Transcriptional Profile of Genes Involved in Glycoalkaloid Biosynthesis in New Varieties of Solanum tuberosum L.

Before commercial release, new potato (Solanum tuberosum) varieties must be evaluated for content of toxic compounds such as glycoalkaloids (GAs), which are potent poisons. GA biosynthesis proceeds via the cholesterol pathway to α-chaconine and α-solanine. The goal of this study was to evaluate the relationship between total glycoalkaloid (TGA) content and the expression of GAME, SGT1, and SGT3 genes in potato tubers. TGA content was measured by HPLC-MS, and reverse transcription quantitative polymerase chain reactions were performed to determine the relative expression of GAME, SGT1, and SGT3 genes. We searched for cis-elements of the transcription start site using the PlantPAN database. There was a relationship between TGA content and the relative expression of GAME, SGT1, and SGT3 genes in potato tubers. Putative promoter regions showed the presence of several cis-elements related to biotic and abiotic stresses and light. These findings provide an important step toward understanding TGA regulation and variation in potato tubers.</p

Selection of reference genes for transcriptional analysis of edible tubers of potato (Solanum tuberosum L.)

<p>Potato (Solanum tuberosum) yield has increased dramatically over the last 50 years and this has been achieved by a combination of improved agronomy and biotechnology efforts. Gene studies are taking place to improve new qualities and develop new cultivars. Reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) is a bench-marking analytical tool for gene expression analysis, but its accuracy is highly dependent on a reliable normalization strategy of an invariant reference genes. For this reason, the goal of this work was to select and validate reference genes for transcriptional analysis of edible tubers of potato. To do so, RT-qPCR primers were designed for ten genes with relatively stable expression in potato tubers as observed in RNA-Seq experiments. Primers were designed across exon boundaries to avoid genomic DNA contamination. Differences were observed in the ranking of candidate genes identified by geNorm, NormFinder and BestKeeper algorithms. The ranks determined by geNorm and NormFinder were very similar and for all samples the most stable candidates were C2, exocyst complex component sec3 (SEC3) and ATCUL3/ATCUL3A/CUL3/CUL3A (CUL3A). According to BestKeeper, the importin alpha and ubiquitin-associated/ts-n genes were the most stable. Three genes were selected as reference genes for potato edible tubers in RT-qPCR studies. The first one, called C2, was selected in common by NormFinder and geNorm, the second one is SEC3, selected by NormFinder, and the third one is CUL3A, selected by geNorm. Appropriate reference genes identified in this work will help to improve the accuracy of gene expression quantification analyses by taking into account differences that may be observed in RNA quality or reverse transcription efficiency across the samples.</p