Microbial community analysis of fouled reverse osmosis membranes used in water recycling

Biofouling on RO membranes has major cost implications in water reclamation. In this study membranes and water samples were collected from a RO pilot-plant operated on two sites to study the differences in microbial communities in order to develop a better understanding of the biofouling. For the two sites studied, the examination of the front membrane of the first stage and the tail membrane of the second stage of the RO train using 16S rRNA gene-based molecular technique showed that bacteria were similar on both stages and no significant effect of the membrane location within the RO train on the biofilm development could be discerned. However, the comparison of the identified bacteria from membrane samples between the two sites showed that each site is specific, leading to a different composition of microbial communities. The different nutrient concentrations in the RO feed water due to the different biological pre-treatments are one potential explanation for the observed differences in the microbial communities. Seasonal variations also play a major role in the development of microbial communities as shown by the significant differences observed between the communities measured in the samples in winter and summer on the second site. The results did not show similarity between the species identified on the RO membranes and in the feed water. Hence, the relationship of microbial community between the water generated during the pre-treatment process and RO membranes is not obvious. From this study, results showed that there is an actual need to investigate the development of microbial communities on membrane surface in real conditions in order to suggest tailored solutions for biofouling control and removal

Characterization of humic acid reactivity modifications due to adsorption onto α-Al2_2O3_3

International audienceAdsorption of purified Aldrich humic acid (PAHA) onto a-Al2O3 is studied by batch experiments at different pH, ionic strength and coverage ratios R (mg of PAHA by m2 of mineral surface). After equilibration, samples are centrifuged and the concentration of PAHA in the supernatants is measured. The amount of adsorbed PAHA per m2 of mineral surface is decreasing with increasing pH. At constant pH value, the amount of adsorbed PAHA increases with initial PAHA concentration until a pH-dependent constant value is reached. UV/Visible specific parameters such as specific absorbance SUVA254, ratio of absorbance values E2/E3 and width of the electron-transfer absorbance band ΔET are calculated for supernatant PAHA fractions of adsorption experiments at pH 6.8, to have an insight on the evolution of PAHA characteristics with varying coverage ratio. No modification is observed compared to original compound for Rs20 mgPAHA=ga_Al2O3.. Below this ratio, aromaticity decreases with initial PAHA concentration. Size-exclusion chromatography e organic carbon detection measurements on these supernatants also show a preferential adsorption of more aromatic and higher-sized fractions. Spectrophotometric titrations were done to estimate changes of reactivity of supernatants from adsorption experiments made at pH z6.8 and different PAHA concentrations. Evolutions of UV/Visible spectra with varying pH were treated to obtain titration curves that are interpreted within the NICA-Donnan framework. Protonation parameters of nonsorbed PAHA fractions are compared to those obtained for the PAHA before contact with the oxide. The amount of low proton-affinity type of sites and the value of their median affinity constant decrease after adsorption. From PAHA concentration in the supernatant and mass balance calculations, "titration curves" are experimentally proposed for the adsorbed fractions for the first time. These changes in reactivity to our opinion could explain the difficulty to model the behavior of ternary systems composed of pollutants/HS/mineral since additivity is not respected

Characterization of humic acid reactivity modifications due to adsorption onto α-Al2O3

International audienceAdsorption of purified Aldrich humic acid (PAHA) onto a-Al2O3 is studied by batch experiments at different pH, ionic strength and coverage ratios R (mg of PAHA by m2 of mineral surface). After equilibration, samples are centrifuged and the concentration of PAHA in the supernatants is measured. The amount of adsorbed PAHA per m2 of mineral surface is decreasing with increasing pH. At constant pH value, the amount of adsorbed PAHA increases with initial PAHA concentration until a pH-dependent constant value is reached. UV/Visible specific parameters such as specific absorbance SUVA254, ratio of absorbance values E2/E3 and width of the electron-transfer absorbance band ΔET are calculated for supernatant PAHA fractions of adsorption experiments at pH 6.8, to have an insight on the evolution of PAHA characteristics with varying coverage ratio. No modification is observed compared to original compound for Rs20 mgPAHA=ga_Al2O3.. Below this ratio, aromaticity decreases with initial PAHA concentration. Size-exclusion chromatography e organic carbon detection measurements on these supernatants also show a preferential adsorption of more aromatic and higher-sized fractions. Spectrophotometric titrations were done to estimate changes of reactivity of supernatants from adsorption experiments made at pH z6.8 and different PAHA concentrations. Evolutions of UV/Visible spectra with varying pH were treated to obtain titration curves that are interpreted within the NICA-Donnan framework. Protonation parameters of nonsorbed PAHA fractions are compared to those obtained for the PAHA before contact with the oxide. The amount of low proton-affinity type of sites and the value of their median affinity constant decrease after adsorption. From PAHA concentration in the supernatant and mass balance calculations, "titration curves" are experimentally proposed for the adsorbed fractions for the first time. These changes in reactivity to our opinion could explain the difficulty to model the behavior of ternary systems composed of pollutants/HS/mineral since additivity is not respected

Adsorption of humic acid onto a kaolinitic clay studied by high-resolution argon adsorption volumetry

28 ref. doi: 10.1180/0009855033840107International audienc

Cutaneous phaeohyphomycosis due to Alternaria infectoria36745

Here we report a case of cutaneous alternariosis in a 74-year-old man treated by corticotherapy for myasthenia, and presenting with papular, crusted lesions on the left elbow and the right knee. Histological examination of the biopsy specimens showed fungal hyphae associated with round-shaped cells which were highly suggestive of alternariosis. Mycological culture allowed the isolation of a dematiaceous fungus which was identified as a member of the Alternaria infectoria species-group. This was confirmed by PCR amplification and sequencing of the internal transcribed spacer domain of the gene encoding nuclear ribosomal DNA and of the mitochondrial small subunit ribosomal DNA domain. The fungus was therefore referred to the Scientific Institute of Public Health where it was identified as Alternaria infectoria, on the basis of its very small 1 or 2-celled conidia often arranged in long chains and presenting with very long secondary conidiophores. Corticotherapy was stopped and a local antifungal treatment with ketoconazole was initiated, allowing the stabilisation of the cutaneous lesions within 2 months</p

Effects of charging on the chromophores of dissolved organic matter from the Rio Negro basin

This study demonstrates that the deprotonation of dissolved organic matter (DOM) originating from a small creek characteristic for DOM-rich waters located in the Rio Negro basin can be quantified based on measurements of pH effects on its absorbance spectra. The method was ascertained by the data of Near-Edge X-Ray Absorbance Spectroscopy (NEXAFS), potentiometric titration to quantify the structural and compositional differences between the colloidal and hydrophobic fractions that contribute 91% of black-water creek DOM. Changes in the absorbance spectra of the DOM fractions caused by deprotonation quantified via numeric deconvolution which indicated the presence of six well-resolved Gaussian bands in the differential spectra. The emergence of these bands was determined to be associated with the engagement of carboxylic and phenolic functionalities and changes of inter-chromophore interactions in DOM molecules. Interpretation of the data based on the NICA-Donnan approach showed that behavior of DOM chromophores was consistent with results of potentiometric titrations. Similar trends were observed for changes of the spectral slope of the DOM absorbance spectra in the range of wavelengths 325e375 nm (DSlope325e375). The behavior of DSlope325e375 values was modeled based on the NICA-Donnan approach and correlated with potentiometrically-estimated charge