110 research outputs found
Recommended from our members
SUMOylation Is Required for Glycine-Induced Increases in AMPA Receptor Surface Expression (ChemLTP) in Hippocampal Neurons
yesMultiple pathways participate in the AMPA receptor trafficking that underlies long-term potentiation (LTP) of synaptic transmission. Here we demonstrate that protein SUMOylation is required for insertion of the GluA1 AMPAR subunit following transient glycine-evoked increase in AMPA receptor surface expression (ChemLTP) in dispersed neuronal cultures. ChemLTP increases co-localisation of SUMO-1 and the SUMO conjugating enzyme Ubc9 and with PSD95 consistent with the recruitment of SUMOylated proteins to dendritic spines. In addition, we show that ChemLTP increases dendritic levels of SUMO-1 and Ubc9 mRNA. Consistent with activity dependent translocation of these mRNAs to sites near synapses, levels of the mRNA binding and dendritic transport protein CPEB are also increased by ChemLTP. Importantly, reducing the extent of substrate protein SUMOylation by overexpressing the deSUMOylating enzyme SENP-1 or inhibiting SUMOylation by expressing dominant negative Ubc9 prevent the ChemLTP-induced increase in both AMPAR surface expression and dendritic SUMO-1 mRNA. Taken together these data demonstrate that SUMOylation of synaptic protein(s) involved in AMPA receptor trafficking is necessary for activity-dependent increases in AMPAR surface expression.Medical Research Council, the European Research Council and the Wellcome Trus
Synchronization and Coarsening (without SOC) in a Forest-Fire Model
We study the long-time dynamics of a forest-fire model with deterministic
tree growth and instantaneous burning of entire forests by stochastic lightning
strikes. Asymptotically the system organizes into a coarsening self-similar
mosaic of synchronized patches within which trees regrow and burn
simultaneously. We show that the average patch length grows linearly with
time as t-->oo. The number density of patches of length L, N(L,t), scales as
^{-2}M(L/), and within a mean-field rate equation description we find
that this scaling function decays as e^{-1/x} for x-->0, and as e^{-x} for
x-->oo. In one dimension, we develop an event-driven cluster algorithm to study
the asymptotic behavior of large systems. Our numerical results are consistent
with mean-field predictions for patch coarsening.Comment: 5 pages, 4 figures, 2-column revtex format. To be submitted to PR
Intrinsic quadrupole moment of the nucleon
We address the question of the intrinsic quadrupole moment Q_0 of the nucleon
in various models. All models give a positive intrinsic quadrupole moment for
the proton. This corresponds to a prolate deformation. We also calculate the
intrinsic quadrupole moment of the Delta(1232). All our models lead to a
negative intrinsic quadrupole moment of the Delta corresponding to an oblate
deformation.Comment: 17 pages, 5 figure
Implementations of a model of physical sorting
We define a computational model of physical devices that
have a parallel atomic operation that transforms their input, an unordered
list, in such a way that their output, the sorted list, can be sequentially
read off in linear time. We show that several commonly-used
scientific laboratory techniques (from biology, chemistry, and physics) are
instances of the model and we provide experimental implementations
Implementations of a model of physical sorting
We define a computational model of physical devices that
have a parallel atomic operation that transforms their input, an unordered
list, in such a way that their output, the sorted list, can be sequentially
read off in linear time. We show that several commonly-used
scientific laboratory techniques (from biology, chemistry, and physics) are
instances of the model and we provide experimental implementations
Surface Magnetization of Aperiodic Ising Quantum Chains
We study the surface magnetization of aperiodic Ising quantum chains. Using
fermion techniques, exact results are obtained in the critical region for
quasiperiodic sequences generated through an irrational number as well as for
the automatic binary Thue-Morse sequence and its generalizations modulo p. The
surface magnetization exponent keeps its Ising value, beta_s=1/2, for all the
sequences studied. The critical amplitude of the surface magnetization depends
on the strength of the modulation and also on the starting point of the chain
along the aperiodic sequence.Comment: 11 pages, 6 eps-figures, Plain TeX, eps
Stripes and holes in a two-dimensional model of spinless fermions and hardcore bosons
We consider a Hubbard-like model of strongly-interacting spinless fermions
and hardcore bosons on a square lattice, such that nearest neighbor occupation
is forbidden. Stripes (lines of holes across the lattice forming antiphase
walls between ordered domains) are a favorable way to dope this system below
half-filling. The problem of a single stripe can be mapped to a spin-1/2 chain,
which allows understanding of its elementary excitations and calculation of the
stripe's effective mass for transverse vibrations. Using Lanczos exact
diagonalization, we investigate the excitation gap and dispersion of a hole on
a stripe, and the interaction of two holes. We also study the interaction of
two, three, and four stripes, finding that they repel, and the interaction
energy decays with stripe separation as if they are hardcore particles moving
in one (transverse) direction. To determine the stability of an array of
stripes against phase separation into particle-rich phase and hole-rich liquid,
we evaluate the liquid's equation of state, finding the stripe-array is not
stable for bosons but is possibly stable for fermions.Comment: 24 pages, 18 figure
SENP3 promotes an Mff-primed Bcl-xL-Drp1 interaction involved in cell death following ischemia
Dysregulation of the mitochondrial fission machinery has been linked to cell death following ischemia. Fission is largely dependent on recruitment of Dynamin-related protein 1 (Drp1) to the receptor Mitochondrial fission factor (Mff) located on the mitochondrial outer membrane (MOM). Drp1 is a target for SUMOylation and its deSUMOylation, mediated by the SUMO protease SENP3, enhances the Drp1-Mff interaction to promote cell death in an oxygen/glucose deprivation (OGD) model of ischemia. Another interacting partner for Drp1 is the Bcl-2 family member Bcl-xL, an important protein in cell death and survival pathways. Here we demonstrate that preventing Drp1 SUMOylation by mutating its SUMO target lysines enhances the Drp1-Bcl-xL interaction in vivo and in vitro. Moreover, SENP3-mediated deSUMOylation of Drp1 promotes the Drp1-Bcl-xL interaction. Our data suggest that Mff primes Drp1 binding to Bcl-xL at the mitochondria and that Mff and Bcl-xL can interact directly, independent of Drp1, through their transmembrane domains. Importantly, SENP3 loss in cells subjected to OGD correlates with reduced Drp1-Bcl-xL interaction, whilst recovery of SENP3 levels in cells subjected to reoxygenation following OGD correlates with increased Drp1-Bcl-xL interaction. Expressing a Bcl-xL mutant with defective Drp1 binding reduces OGD plus reoxygenation-evoked cell death. Taken together, our results indicate that SENP3-mediated deSUMOlyation promotes an Mff-primed Drp1-Bcl-xL interaction that contributes to cell death following ischemia
- …